Restriction map for
pF1KA1310
(Circular) (Six-base) MAPSORT of: pF1KA1310.seq Check: 5951 from: 1 to: 5453
pF1KA1310 5453 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2113 2113
Size: 5453
AatII G_ACGT'C
Cuts at: 335 335
Size: 5453
AccI GT'mk_AC
Cuts at: 1641 2491 1641
Size: 850 4603
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5453
AclI AA'CG_TT
Cuts at: 5293 5293
Size: 5453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 781 874 1367 1816 2005 2144 2231 2237
Size: 653 93 493 449 189 139 87 6
Cuts at: 2237 2358 3278 3710 4063 4725 128
Size: 121 920 432 353 662 856
Fragments arranged by size:
920 856 662 653 493 449 432 353
189 139 121 93 87 6
AflIII A'CryG_T
Cuts at: 1803 2805 4605 4886 1803
Size: 1002 1800 281 2370
Fragments arranged by size:
2370 1800 1002 281
AhdI GACnn_n'nnGTC
Cuts at: 716 716
Size: 5453
AleI CACnn'nnGTG
Cuts at: 119 703 119
Size: 584 4869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 1242 184
Size: 1058 4395
AlwNI CAG_nnn'CTG
Cuts at: 651 690 759 832 1794 1822 2119 2758 4196
Size: 39 69 73 962 28 297 639 1438
Cuts at: 4196 651
Size: 1908
Fragments arranged by size:
1908 1438 962 639 297 73 69 39
28
ApaLI G'TGCA_C
Cuts at: 4291 4291
Size: 5453
ApoI r'AATT_y
Cuts at: 1326 2457 4806 5062 1326
Size: 1131 2349 256 1717
Fragments arranged by size:
2349 1717 1131 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 1081 1556 2001 2473 1081
Size: 475 445 472 4061
Fragments arranged by size:
4061 475 472 445
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 300 333 300
Size: 33 5420
BamHI G'GATC_C
Cuts at: 2478 2478
Size: 5453
BanI G'GyrC_C
Cuts at: 160 1906 2469 2883 3131 3166 160
Size: 1746 563 414 248 35 2447
Fragments arranged by size:
2447 1746 563 414 248 35
BanII G_rGCy'C
Cuts at: 1549 2180 2467 3497 1549
Size: 631 287 1030 3505
Fragments arranged by size:
3505 1030 631 287
BbeI G_GCGC'C
Cuts at: 2887 3135 2887
Size: 248 5205
BbsI GAAGACnn'nnnn_
Cuts at: 1303 1303
Size: 5453
BbvCI CC'TCA_GC
Cuts at: 833 1532 2368 833
Size: 699 836 3918
Fragments arranged by size:
3918 836 699
BciVI GTATCCnnnnn_n'
Cuts at: 663 3351 4407 5453 663
Size: 2688 1056 1046 663
Fragments arranged by size:
2688 1056 1046 663
BclI T'GATC_A
Cuts at: 2973 2973
Size: 5453
BglI GCCn_nnn'nGGC
Cuts at: 571 2443 571
Size: 1872 3581
BglII A'GATC_T
Cuts at: 2968 5005 2968
Size: 2037 3416
BlpI GC'TnA_GC
Cuts at: 2565 2565
Size: 5453
Bme1580I G_kGCm'C
Cuts at: 163 249 270 3078 3171 4295 163
Size: 86 21 2808 93 1124 1321
Fragments arranged by size:
2808 1321 1124 93 86 21
BmrI ACTGGGnnnn_n'
Cuts at: 483 827 2158 2233 2267 2665 2746 3081 5225
Size: 344 1331 75 34 398 81 335 2144
Cuts at: 5225 483
Size: 711
Fragments arranged by size:
2144 1331 711 398 344 335 81 75
34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1713 1910 3820 1713
Size: 197 1910 3346
Fragments arranged by size:
3346 1910 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 936 1138 1286 1535 1683 1707 2096 2099 2241
Size: 202 148 249 148 24 389 3 142
Cuts at: 2241 936
Size: 4148
Fragments arranged by size:
4148 389 249 202 148 148 142 24
3
Bpu10I CC'TnA_GC
Cuts at: 833 1532 1628 2368 833
Size: 699 96 740 3918
Fragments arranged by size:
3918 740 699 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 1829 2629 3508 4222 4520 735
Size: 1094 800 879 714 298 1668
Fragments arranged by size:
1668 1094 879 800 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 703 2259 5157 703
Size: 1556 2898 999
Fragments arranged by size:
2898 1556 999
BsaAI yAC'GTr
Cuts at: 2806 2806
Size: 5453
BsaBI GATnn'nnATC
Cuts at: 6 931 1418 2967 5004 6
Size: 925 487 1549 2037 455
Fragments arranged by size:
2037 1549 925 487 455
BsaHI Gr'CG_yC
Cuts at: 332 2884 3132 3834 5330 332
Size: 2552 248 702 1496 455
Fragments arranged by size:
2552 1496 702 455 248
BsaWI w'CCGG_w
Cuts at: 788 2642 3163 3841 4252 4399 4673 788
Size: 1854 521 678 411 147 274 1568
Fragments arranged by size:
1854 1568 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1166 1196 1166
Size: 30 5423
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 493 930 1902 2347 449
Size: 44 437 972 445 3555
Fragments arranged by size:
3555 972 445 437 44
BseYI C'CCAG_C
Cuts at: 665 1653 2878 3636 4301 4709 665
Size: 988 1225 758 665 408 1409
Fragments arranged by size:
1409 1225 988 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1828 2252 676
Size: 1152 424 3877
Fragments arranged by size:
3877 1152 424
BsiEI CG_ry'CG
Cuts at: 104 2038 2995 3041 4271 104
Size: 1934 957 46 1230 1286
Fragments arranged by size:
1934 1286 1230 957 46
BsiHKAI G_wGCw'C
Cuts at: 1523 2094 2112 2180 2467 3245 3435 4295 1523
Size: 571 18 68 287 778 190 860 2681
Fragments arranged by size:
2681 860 778 571 287 190 68 18
BsmBI CGTCTCn'nnnn_
Cuts at: 343 343
Size: 5453
Bsp1286I G_dGCh'C
Cuts at: 163 249 270 1523 1549 2094 2112 2180 2467
Size: 86 21 1253 26 545 18 68 287
Cuts at: 2467 3078 3171 3245 3435 3497 4295 163
Size: 611 93 74 190 62 798 1321
Fragments arranged by size:
1321 1253 798 611 545 287 190 93
86 74 68 62 26 21 18
BspHI T'CATG_A
Cuts at: 3885 3885
Size: 5453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1653 2113 2503 2717 3019 3400 1653
Size: 460 390 214 302 381 3706
Fragments arranged by size:
3706 460 390 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1632 3745 3799 5283 5448 50
Size: 1582 2113 54 1484 165 55
Fragments arranged by size:
2113 1582 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3365 3365
Size: 5453
BsrFI r'CCGG_y
Cuts at: 557 3451 3632 557
Size: 2894 181 2378
Fragments arranged by size:
2894 2378 181
BssSI C'ACGA_G
Cuts at: 1526 3724 4432 1526
Size: 2198 708 2547
Fragments arranged by size:
2547 2198 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1855 2275 2289 2830 1855
Size: 420 14 541 4478
Fragments arranged by size:
4478 541 420 14
BstBI TT'CG_AA
Cuts at: 3814 3814
Size: 5453
BstXI CCAn_nnnn'nTGG
Cuts at: 121 473 705 2148 121
Size: 352 232 1443 3426
Fragments arranged by size:
3426 1443 352 232
BstYI r'GATC_y
Cuts at: 1 533 770 1692 1968 2478 2945 2968 3303
Size: 532 237 922 276 510 467 23 335
Cuts at: 3303 3549 3855 3867 3953 5005 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 922 532 510 467 449 335 306
276 246 237 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1680 1680
Size: 5453
BtgI C'CryG_G
Cuts at: 107 1352 107
Size: 1245 4208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 918 1607 3403 3584 3747 918
Size: 689 1796 181 163 2624
Fragments arranged by size:
2624 1796 689 181 163
BtsI GCAGTG_nn'
Cuts at: 1062 1106 1214 2264 2287 2696 1062
Size: 44 108 1050 23 409 3819
Fragments arranged by size:
3819 1050 409 108 44 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 326 361 1038 1073 326
Size: 35 677 35 4706
Fragments arranged by size:
4706 677 35 35
DraI TTT'AAA
Cuts at: 2452 2452
Size: 5453
DraIII CAC_nnn'GTG
Cuts at: 2275 2275
Size: 5453
DrdI GACnn_nn'nnGTC
Cuts at: 3159 4503 3159
Size: 1344 4109
EaeI y'GGCC_r
Cuts at: 572 2306 3038 3212 3603 3630 4975 572
Size: 1734 732 174 391 27 1345 1050
Fragments arranged by size:
1734 1345 1050 732 391 174 27
EagI C'GGCC_G
Cuts at: 3038 3038
Size: 5453
EarI CTCTTCn'nnn_
Cuts at: 178 850 1792 1901 3476 3686 178
Size: 672 942 109 1575 210 1945
Fragments arranged by size:
1945 1575 942 672 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 4403 4549 5263 3661
Size: 742 146 714 3851
Fragments arranged by size:
3851 742 714 146
EcoICRI GAG'CTC
Cuts at: 2178 2465 2178
Size: 287 5166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 781 874 936 1138 1286 1367 1535 1683
Size: 653 93 62 202 148 81 168 148
Cuts at: 1683 1707 1816 2005 2096 2099 2144 2231 2237
Size: 24 109 189 91 3 45 87 6
Cuts at: 2237 2241 2358 3278 3710 4063 4725 128
Size: 4 117 920 432 353 662 856
Fragments arranged by size:
920 856 662 653 432 353 202 189
168 148 148 117 109 93 91 87
81 62 45 24 6 4 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2099
Size: 5453
EcoO109I rG'GnC_Cy
Cuts at: 860 867 2083 2592 860
Size: 7 1216 509 3721
Fragments arranged by size:
3721 1216 509 7
EcoRI G'AATT_C
Cuts at: 1326 2457 1326
Size: 1131 4322
EcoRV GAT'ATC
Cuts at: 2388 2388
Size: 5453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1987 3472 1987
Size: 1485 3968
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5453
HaeII r_GCGC'y
Cuts at: 2887 3135 4365 5136 2887
Size: 248 1230 771 3204
Fragments arranged by size:
3204 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 702 734 826 858 2027 2059 3295
Size: 32 538 32 92 32 1169 32 1236
Cuts at: 3295 3325 3327 3357 132
Size: 30 2 30 2228
Fragments arranged by size:
2228 1236 1169 538 92 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 489 1460 1642 2492 489
Size: 971 182 850 3450
Fragments arranged by size:
3450 971 850 182
KasI G'GCGC_C
Cuts at: 2883 3131 2883
Size: 248 5205
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5453
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 830 4212 4396 830
Size: 3382 184 1887
Fragments arranged by size:
3382 1887 184
MscI TGG'CCA
Cuts at: 574 2308 3214 574
Size: 1734 906 2813
Fragments arranged by size:
2813 1734 906
MslI CAynn'nnrTG
Cuts at: 119 227 254 280 703 2140 3442 3569 119
Size: 108 27 26 423 1437 1302 127 2003
Fragments arranged by size:
2003 1437 1302 423 127 108 27 26
MspA1I CmG'CkG
Cuts at: 924 1002 1657 1791 1812 1983 2077 2564 2878
Size: 78 655 134 21 171 94 487 314
Cuts at: 2878 3238 4022 4267 924
Size: 360 784 245 2110
Fragments arranged by size:
2110 784 655 487 360 314 245 171
134 94 78 21
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5453
NarI GG'CG_CC
Cuts at: 2884 3132 2884
Size: 248 5205
NcoI C'CATG_G
Cuts at: 107 1352 107
Size: 1245 4208
NdeI CA'TA_TG
Cuts at: 1088 1088
Size: 5453
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5453
NspI r_CATG'y
Cuts at: 285 427 487 1807 2506 3006 4609 285
Size: 142 60 1320 699 500 1603 1129
Fragments arranged by size:
1603 1320 1129 699 500 142 60
PasI CC'CwG_GG
Cuts at: 863 2332 863
Size: 1469 3984
PciI A'CATG_T
Cuts at: 1803 4605 1803
Size: 2802 2651
PflMI CCAn_nnn'nTGG
Cuts at: 239 239
Size: 5453
PfoI T'CCnGG_A
Cuts at: 1364 1364
Size: 5453
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 5421
PpuMI rG'GwC_Cy
Cuts at: 867 867
Size: 5453
PshAI GACnn'nnGTC
Cuts at: 1303 1303
Size: 5453
PspXI vC'TCGA_Gb
Cuts at: 2001 2001
Size: 5453
PstI C_TGCA'G
Cuts at: 551 828 1221 2124 2294 2500 3185 551
Size: 277 393 903 170 206 685 2819
Fragments arranged by size:
2819 903 685 393 277 206 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 1002 1657 1791 1983 2077 2878 3238 1002
Size: 655 134 192 94 801 360 3217
Fragments arranged by size:
3217 801 655 360 192 134 94
RsrII CG'GwC_CG
Cuts at: 3648 3648
Size: 5453
SacI G_AGCT'C
Cuts at: 2180 2467 2180
Size: 287 5166
SalI G'TCGA_C
Cuts at: 1640 2490 1640
Size: 850 4603
SanDI GG'GwC_CC
Cuts at: 867 867
Size: 5453
SapI GCTCTTCn'nnn_
Cuts at: 178 1901 3476 3686 178
Size: 1723 1575 210 1945
Fragments arranged by size:
1945 1723 1575 210
SbfI CC_TGCA'GG
Cuts at: 2500 2500
Size: 5453
ScaI AGT'ACT
Cuts at: 4970 4970
Size: 5453
SexAI A'CCwGG_T
Cuts at: 206 206
Size: 5453
SfcI C'TryA_G
Cuts at: 33 547 824 1028 1072 1217 2120 2290 2496
Size: 514 277 204 44 145 903 170 206
Cuts at: 2496 3181 4149 4340 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 903 685 514 277 206 204
191 170 145 44
SfoI GGC'GCC
Cuts at: 2885 3133 2885
Size: 248 5205
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SmaI CCC'GGG
Cuts at: 2475 2475
Size: 5453
SmlI C'TyrA_G
Cuts at: 714 1081 1844 2001 2608 3523 4237 4499 714
Size: 367 763 157 607 915 714 262 1668
Fragments arranged by size:
1668 915 763 714 607 367 262 157
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5453
SphI G_CATG'C
Cuts at: 285 2506 3006 285
Size: 2221 500 2732
Fragments arranged by size:
2732 2221 500
StuI AGG'CCT
Cuts at: 838 2366 838
Size: 1528 3925
StyI C'CwwG_G
Cuts at: 107 238 1230 1352 2064 2100 2171 2361 2587
Size: 131 992 122 712 36 71 190 226
Cuts at: 2587 2941 107
Size: 354 2619
Fragments arranged by size:
2619 992 712 354 226 190 131 122
71 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 266 868 1051 2052 2286 3036 3169 3628 3837
Size: 602 183 1001 234 750 133 459 209
Cuts at: 3837 266
Size: 1882
Fragments arranged by size:
1882 1001 750 602 459 234 209 183
133
TatI w'GTAC_w
Cuts at: 4968 4968
Size: 5453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1166 1198 1710 1742 4970 5002 1166
Size: 32 512 32 3228 32 1617
Fragments arranged by size:
3228 1617 512 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3250 3250
Size: 5453
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 470 2948 470
Size: 2478 2975
XhoI C'TCGA_G
Cuts at: 1081 2001 1081
Size: 920 4533
XmaI C'CCGG_G
Cuts at: 2473 2473
Size: 5453
ZraI GAC'GTC
Cuts at: 333 333
Size: 5453
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AloI ApaI AscI AvrII BcgI
BfrBI BmgBI BmtI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstZ17I ClaI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PmlI PsiI PspOMI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI