Restriction map for
pF1KA1305
(Circular) (Six-base) MAPSORT of: pF1KA1305.seq Check: 8465 from: 1 to: 8807
pF1KA1305 8807 bp
With 182 enzymes: SgfI *
December 13, 2010 18:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2025 2085 2103 2157 2390 2025
Size: 60 18 54 233 8442
Fragments arranged by size:
8442 233 60 54 18
AatII G_ACGT'C
Cuts at: 5471 5471
Size: 8807
AccI GT'mk_AC
Cuts at: 1404 4936 5845 1404
Size: 3532 909 4366
Fragments arranged by size:
4366 3532 909
Acc65I G'GTAC_C
Cuts at: 2417 5823 2417
Size: 3406 5401
AclI AA'CG_TT
Cuts at: 8647 8647
Size: 8807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 2269 2650 2959 3001 3550 4236 5080 5083
Size: 1935 381 309 42 549 686 844 3
Cuts at: 5083 5334 6632 7064 7811 8079 334
Size: 251 1298 432 747 268 1062
Fragments arranged by size:
1935 1298 1062 844 747 686 549 432
381 309 268 251 42 3
AfeI AGC'GCT
Cuts at: 4198 4198
Size: 8807
AflIII A'CryG_T
Cuts at: 4040 6159 7263 8240 4040
Size: 2119 1104 977 4607
Fragments arranged by size:
4607 2119 1104 977
AgeI A'CCGG_T
Cuts at: 7195 7195
Size: 8807
AleI CACnn'nnGTG
Cuts at: 3787 5080 3787
Size: 1293 7514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3359 5126 3359
Size: 1767 7040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5218 5250 5218
Size: 32 8775
AlwNI CAG_nnn'CTG
Cuts at: 192 669 1711 1729 1855 3939 3999 4608 4706
Size: 477 1042 18 126 2084 60 609 98
Cuts at: 4706 4862 5094 6112 7679 192
Size: 156 232 1018 1567 1320
Fragments arranged by size:
2084 1567 1320 1042 1018 609 477 232
156 126 98 60 18
ApaI G_GGCC'C
Cuts at: 281 390 541 603 871 1303 2361 3053 3261
Size: 109 151 62 268 432 1058 692 208
Cuts at: 3261 3457 3712 3753 3915 4773 4978 5156 5283
Size: 196 255 41 162 858 205 178 127
Cuts at: 5283 281
Size: 3805
Fragments arranged by size:
3805 1058 858 692 432 268 255 208
205 196 178 162 151 127 109 62
41
ApaLI G'TGCA_C
Cuts at: 1843 7577 1843
Size: 5734 3073
ApoI r'AATT_y
Cuts at: 876 5269 5811 8160 8416 876
Size: 4393 542 2349 256 1267
Fragments arranged by size:
4393 2349 1267 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8807
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8807
AvaI C'yCGr_G
Cuts at: 173 271 281 560 587 1161 4289 5827 173
Size: 98 10 279 27 574 3128 1538 3153
Fragments arranged by size:
3153 3128 1538 574 279 98 27 10
BamHI G'GATC_C
Cuts at: 611 5832 611
Size: 5221 3586
BanI G'GyrC_C
Cuts at: 438 2163 2293 2417 2889 3227 4450 5770 5823
Size: 1725 130 124 472 338 1223 1320 53
Cuts at: 5823 6237 6485 6520 438
Size: 414 248 35 2725
Fragments arranged by size:
2725 1725 1320 1223 472 414 338 248
130 124 53 35
BanII G_rGCy'C
Cuts at: 281 390 541 603 871 1303 1504 1719 2139
Size: 109 151 62 268 432 201 215 420
Cuts at: 2139 2339 2361 3053 3261 3457 3540 3712 3753
Size: 200 22 692 208 196 83 172 41
Cuts at: 3753 3915 4271 4290 4317 4411 4773 4978 5156
Size: 162 356 19 27 94 362 205 178
Cuts at: 5156 5205 5254 5283 5420 5459 5507 5821 6851
Size: 49 49 29 137 39 48 314 1030
Cuts at: 6851 281
Size: 2237
Fragments arranged by size:
2237 1030 692 432 420 362 356 314
268 215 208 205 201 200 196 178
172 162 151 137 109 94 83 62
49 49 48 41 39 29 27 22
19
BbeI G_GCGC'C
Cuts at: 6241 6489 6241
Size: 248 8559
BbsI GAAGACnn'nnnn_
Cuts at: 713 2998 5463 713
Size: 2285 2465 4057
Fragments arranged by size:
4057 2465 2285
BbvCI CC'TCA_GC
Cuts at: 578 2305 3149 3232 578
Size: 1727 844 83 6153
Fragments arranged by size:
6153 1727 844 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2356 2390 4564 4598 90
Size: 34 2232 34 2174 34 4299
Fragments arranged by size:
4299 2232 2174 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6705 7466 8807 6705
Size: 761 1341 6705
Fragments arranged by size:
6705 1341 761
BclI T'GATC_A
Cuts at: 917 6327 917
Size: 5410 3397
BglI GCCn_nnn'nGGC
Cuts at: 452 598 734 1185 1662 2915 3352 3489 3523
Size: 146 136 451 477 1253 437 137 34
Cuts at: 3523 3622 4171 5209 5353 452
Size: 99 549 1038 144 3906
Fragments arranged by size:
3906 1253 1038 549 477 451 437 146
144 137 136 99 34
BglII A'GATC_T
Cuts at: 6322 8359 6322
Size: 2037 6770
BlpI GC'TnA_GC
Cuts at: 676 724 3009 5674 5919 676
Size: 48 2285 2665 245 3564
Fragments arranged by size:
3564 2665 2285 245 48
Bme1580I G_kGCm'C
Cuts at: 281 390 541 603 871 1141 1303 1748 1766
Size: 109 151 62 268 270 162 445 18
Cuts at: 1766 1847 2168 2361 3053 3136 3202 3261 3457
Size: 81 321 193 692 83 66 59 196
Cuts at: 3457 3712 3753 3915 4455 4773 4978 5156 5283
Size: 255 41 162 540 318 205 178 127
Cuts at: 5283 6432 6525 7581 281
Size: 1149 93 1056 1507
Fragments arranged by size:
1507 1149 1056 692 540 445 321 318
270 268 255 205 196 193 178 162
162 151 127 109 93 83 81 66
62 59 41 18
BmgBI CAC'GTC
Cuts at: 5172 5172
Size: 8807
BmrI ACTGGGnnnn_n'
Cuts at: 917 2277 3708 4213 5151 5274 6019 6100 6435
Size: 1360 1431 505 938 123 745 81 335
Cuts at: 6435 8579 917
Size: 2144 1145
Fragments arranged by size:
2144 1431 1360 1145 938 745 505 335
123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7174 7174
Size: 8807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 529 841 1100 1233 1369 1601 1631 1733
Size: 91 312 259 133 136 232 30 102
Cuts at: 1733 1767 2115 3076 3613 3916 4213 4302 5158
Size: 34 348 961 537 303 297 89 856
Cuts at: 5158 5215 5521 5532 438
Size: 57 306 11 3713
Fragments arranged by size:
3713 961 856 537 348 312 306 303
297 259 232 136 133 102 91 89
57 34 30 11
Bpu10I CC'TnA_GC
Cuts at: 578 2305 3149 3232 5253 578
Size: 1727 844 83 2021 4132
Fragments arranged by size:
4132 2021 1727 844 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 1667 1775 1937 2027 2668 2734 3457 3739
Size: 681 108 162 90 641 66 723 282
Cuts at: 3739 5269 5443 5983 6862 7354 7652 986
Size: 1530 174 540 879 492 298 2141
Fragments arranged by size:
2141 1530 879 723 681 641 540 492
298 282 174 162 108 90 66
BsaI GGTCTCn'nnnn_
Cuts at: 1284 4096 4783 8511 1284
Size: 2812 687 3728 1580
Fragments arranged by size:
3728 2812 1580 687
BsaAI yAC'GTr
Cuts at: 3612 6160 3612
Size: 2548 6259
BsaBI GATnn'nnATC
Cuts at: 6 2809 6321 8358 6
Size: 2803 3512 2037 455
Fragments arranged by size:
3512 2803 2037 455
BsaHI Gr'CG_yC
Cuts at: 3178 5468 6238 6486 7188 8684 3178
Size: 2290 770 248 702 1496 3301
Fragments arranged by size:
3301 2290 1496 770 702 248
BsaWI w'CCGG_w
Cuts at: 759 2639 4727 5996 6517 7195 7469 7616 8027
Size: 1880 2088 1269 521 678 274 147 411
Cuts at: 8027 759
Size: 1539
Fragments arranged by size:
2088 1880 1539 1269 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 736 766 2527 2557 2985 3015 3187 3217 3671
Size: 30 1761 30 428 30 172 30 454
Cuts at: 3671 3701 4381 4411 5192 5222 5407 5437 5566
Size: 30 680 30 781 30 185 30 129
Cuts at: 5566 5596 736
Size: 30 3947
Fragments arranged by size:
3947 1761 781 680 454 428 185 172
129 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 526 1093 1364 1439 2785 3112 3127 3166 3229
Size: 567 271 75 1346 327 15 39 63
Cuts at: 3229 3460 3526 3676 3712 3919 4293 4608 5008
Size: 231 66 150 36 207 374 315 400
Cuts at: 5008 5349 526
Size: 341 3984
Fragments arranged by size:
3984 1346 567 400 374 341 327 315
271 231 207 150 75 66 63 39
36 15
BseYI C'CCAG_C
Cuts at: 533 654 782 801 1395 1419 2708 3004 3356
Size: 121 128 19 594 24 1289 296 352
Cuts at: 3356 3370 3575 3942 4597 4812 5721 5737 6232
Size: 14 205 367 655 215 909 16 495
Cuts at: 6232 6990 7567 8063 533
Size: 758 577 496 1277
Fragments arranged by size:
1289 1277 909 758 655 594 577 496
495 367 352 296 215 205 128 121
24 19 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 199 220 345 352 1406 2590 3445 5323
Size: 24 21 125 7 1054 1184 855 1878
Cuts at: 5323 175
Size: 3659
Fragments arranged by size:
3659 1878 1184 1054 855 125 24 21
7
BsiEI CG_ry'CG
Cuts at: 104 4940 6349 6395 7603 104
Size: 4836 1409 46 1208 1308
Fragments arranged by size:
4836 1409 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 424 934 1002 1504 1719 1847 2139 3644 4271
Size: 510 68 502 215 128 292 1505 627
Cuts at: 4271 5254 5507 5821 6599 6789 7581 424
Size: 983 253 314 778 190 792 1650
Fragments arranged by size:
1650 1505 983 792 778 627 510 502
314 292 253 215 190 128 68
BsmI GAATG_Cn'
Cuts at: 1230 5050 1230
Size: 3820 4987
Bsp1286I G_dGCh'C
Cuts at: 281 390 424 541 603 871 934 1002 1141
Size: 109 34 117 62 268 63 68 139
Cuts at: 1141 1303 1504 1719 1748 1766 1847 2139 2168
Size: 162 201 215 29 18 81 292 29
Cuts at: 2168 2339 2361 3053 3136 3202 3261 3457 3540
Size: 171 22 692 83 66 59 196 83
Cuts at: 3540 3644 3712 3753 3915 4271 4290 4317 4411
Size: 104 68 41 162 356 19 27 94
Cuts at: 4411 4455 4773 4978 5156 5205 5254 5283 5420
Size: 44 318 205 178 49 49 29 137
Cuts at: 5420 5459 5507 5821 6432 6525 6599 6789 6851
Size: 39 48 314 611 93 74 190 62
Cuts at: 6851 7581 281
Size: 730 1507
Fragments arranged by size:
1507 730 692 611 356 318 314 292
268 215 205 201 196 190 178 171
162 162 139 137 117 109 104 94
93 83 83 81 74 68 68 66
63 62 62 59 49 49 48 44
41 39 34 29 29 29 27 22
19 18
BspEI T'CCGG_A
Cuts at: 759 4727 8027 759
Size: 3968 3300 1539
Fragments arranged by size:
3968 3300 1539
BspHI T'CATG_A
Cuts at: 491 7983 491
Size: 7492 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1068 2011 2025 2085 2103 2157 2390 3327 4964
Size: 943 14 60 18 54 233 937 1637
Cuts at: 4964 5790 5857 6071 6373 6754 1068
Size: 826 67 214 302 381 3121
Fragments arranged by size:
3121 1637 943 937 826 381 302 233
214 67 60 54 18 14
BsrBI CCG'CTC
Cuts at: 50 1645 7099 7153 8637 8802 50
Size: 1595 5454 54 1484 165 55
Fragments arranged by size:
5454 1595 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4580 6719 4580
Size: 2139 6668
BsrFI r'CCGG_y
Cuts at: 5669 6805 6986 7195 5669
Size: 1136 181 209 7281
Fragments arranged by size:
7281 1136 209 181
BssSI C'ACGA_G
Cuts at: 2422 7078 7436 2422
Size: 4656 358 3793
Fragments arranged by size:
4656 3793 358
BstAPI GCAn_nnn'nTGC
Cuts at: 192 2529 4862 6184 192
Size: 2337 2333 1322 2815
Fragments arranged by size:
2815 2337 2333 1322
BstBI TT'CG_AA
Cuts at: 7168 7168
Size: 8807
BstEII G'GTnAC_C
Cuts at: 582 619 3597 4980 5593 582
Size: 37 2978 1383 613 3796
Fragments arranged by size:
3796 2978 1383 613 37
BstXI CCAn_nnnn'nTGG
Cuts at: 338 2290 4462 4542 5043 5646 338
Size: 1952 2172 80 501 603 3499
Fragments arranged by size:
3499 2172 1952 603 501 80
BstYI r'GATC_y
Cuts at: 1 566 611 1530 3218 4247 4734 5832 6299
Size: 565 45 919 1688 1029 487 1098 467
Cuts at: 6299 6322 6657 6903 7915 8001 8013 8359 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1688 1098 1029 1012 919 565 487 467
449 346 335 246 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 3833 4930 3833
Size: 1097 7710
BtgI C'CryG_G
Cuts at: 615 806 815 1676 1748 2168 2516 2561 3968
Size: 191 9 861 72 420 348 45 1407
Cuts at: 3968 4178 4520 4921 615
Size: 210 342 401 4501
Fragments arranged by size:
4501 1407 861 420 401 348 342 210
191 72 45 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6757 6938 7101 6757
Size: 181 163 8463
Fragments arranged by size:
8463 181 163
BtsI GCAGTG_nn'
Cuts at: 1766 1847 2514 2573 3499 3821 4236 4474 5640
Size: 81 667 59 926 322 415 238 1166
Cuts at: 5640 6050 1766
Size: 410 4523
Fragments arranged by size:
4523 1166 926 667 415 410 322 238
81 59
ClaI AT'CG_AT
Cuts at: 2498 2498
Size: 8807
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4647 4682 4647
Size: 35 8772
DraI TTT'AAA
Cuts at: 5806 5806
Size: 8807
DraIII CAC_nnn'GTG
Cuts at: 5039 5039
Size: 8807
DrdI GACnn_nn'nnGTC
Cuts at: 1701 6513 7371 1701
Size: 4812 858 3137
Fragments arranged by size:
4812 3137 858
EaeI y'GGCC_r
Cuts at: 1186 1907 2513 3623 4203 4439 4571 6392 6566
Size: 721 606 1110 580 236 132 1821 174
Cuts at: 6566 6957 6984 8329 1186
Size: 391 27 1345 1664
Fragments arranged by size:
1821 1664 1345 1110 721 606 580 391
236 174 132 27
EagI C'GGCC_G
Cuts at: 6392 6392
Size: 8807
EarI CTCTTCn'nnn_
Cuts at: 403 1111 1628 2887 3083 3174 4781 6830 7040
Size: 708 517 1259 196 91 1607 2049 210
Cuts at: 7040 403
Size: 2170
Fragments arranged by size:
2170 2049 1607 1259 708 517 210 196
91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3577 7015 7325 7471 8617 3577
Size: 3438 310 146 1146 3767
Fragments arranged by size:
3767 3438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1502 1717 2137 4269 5252 5505 5819 1502
Size: 215 420 2132 983 253 314 4490
Fragments arranged by size:
4490 2132 983 420 314 253 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 438 529 841 1100 1233 1369 1601 1631
Size: 104 91 312 259 133 136 232 30
Cuts at: 1631 1733 1767 2115 2269 2650 2959 3001 3076
Size: 102 34 348 154 381 309 42 75
Cuts at: 3076 3550 3613 3916 4213 4236 4302 5080 5083
Size: 474 63 303 297 23 66 778 3
Cuts at: 5083 5158 5215 5334 5521 5532 6632 7064 7811
Size: 75 57 119 187 11 1100 432 747
Cuts at: 7811 8079 334
Size: 268 1062
Fragments arranged by size:
1100 1062 778 747 474 432 381 348
312 309 303 297 268 259 232 187
154 136 133 119 104 102 91 75
75 66 63 57 42 34 30 23
11 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1212 1258 3211 3634 3931 4657 1212
Size: 46 1953 423 297 726 5362
Fragments arranged by size:
5362 1953 726 423 297 46
EcoO109I rG'GnC_Cy
Cuts at: 277 278 318 386 537 538 599 600 636
Size: 1 40 68 151 1 61 1 36
Cuts at: 636 851 867 911 1300 1639 2023 2278 2357
Size: 215 16 44 389 339 384 255 79
Cuts at: 2357 2358 2427 3050 3257 3258 3454 3708 3709
Size: 1 69 623 207 1 196 254 1
Cuts at: 3709 3750 3911 3912 3936 4769 5152 5153 5226
Size: 41 161 1 24 833 383 1 73
Cuts at: 5226 5258 5279 5280 5354 5385 5601 5650 5946
Size: 32 21 1 74 31 216 49 296
Cuts at: 5946 277
Size: 3138
Fragments arranged by size:
3138 833 623 389 384 383 339 296
255 254 216 215 207 196 161 151
79 74 73 69 68 61 49 44
41 40 36 32 31 24 21 16
1 1 1 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 5269 5811 5269
Size: 542 8265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6826 6826
Size: 8807
FspI TGC'GCA
Cuts at: 6588 6588
Size: 8807
HaeII r_GCGC'y
Cuts at: 4200 6241 6489 7511 8490 4200
Size: 2041 248 1022 979 4517
Fragments arranged by size:
4517 2041 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 736 768 2480 2512 2985 3017 3028 3060 3151
Size: 32 1712 32 473 32 11 32 91
Cuts at: 3151 3183 3187 3219 3869 3901 6649 6679 6681
Size: 32 4 32 650 32 2748 30 2
Cuts at: 6681 6711 736
Size: 30 2832
Fragments arranged by size:
2832 2748 1712 650 473 91 32 32
32 32 32 32 32 30 30 11
4 2
HincII GTy'rAC
Cuts at: 4937 5846 4937
Size: 909 7898
HindIII A'AGCT_T
Cuts at: 3863 3863
Size: 8807
KasI G'GCGC_C
Cuts at: 6237 6485 6237
Size: 248 8559
KpnI G_GTAC'C
Cuts at: 2421 5827 2421
Size: 3406 5401
MluI A'CGCG_T
Cuts at: 8240 8240
Size: 8807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 998 1134 1906 2936 7478 7662 998
Size: 136 772 1030 4542 184 2143
Fragments arranged by size:
4542 2143 1030 772 184 136
MscI TGG'CCA
Cuts at: 1909 2515 4205 4441 6568 1909
Size: 606 1690 236 2127 4148
Fragments arranged by size:
4148 2127 1690 606 236
MslI CAynn'nnrTG
Cuts at: 2566 2815 3422 3787 4039 5006 5080 6796 6923
Size: 249 607 365 252 967 74 1716 127
Cuts at: 6923 2566
Size: 4450
Fragments arranged by size:
4450 1716 967 607 365 252 249 127
74
MspA1I CmG'CkG
Cuts at: 183 189 648 658 957 1399 2628 3579 3770
Size: 6 459 10 299 442 1229 951 191
Cuts at: 3770 3970 4554 5484 5778 5918 6232 6592 7605
Size: 200 584 930 294 140 314 360 1013
Cuts at: 7605 7850 183
Size: 245 1140
Fragments arranged by size:
1229 1140 1013 951 930 584 459 442
360 314 299 294 245 200 191 140
10 6
NaeI GCC'GGC
Cuts at: 6988 6988
Size: 8807
NarI GG'CG_CC
Cuts at: 6238 6486 6238
Size: 248 8559
NcoI C'CATG_G
Cuts at: 806 1748 2516 2561 4520 4921 806
Size: 942 768 45 1959 401 4692
Fragments arranged by size:
4692 1959 942 768 401 45
NgoMIV G'CCGG_C
Cuts at: 6986 6986
Size: 8807
NspI r_CATG'y
Cuts at: 631 4044 4843 5011 5107 5860 6360 7267 631
Size: 3413 799 168 96 753 500 907 2171
Fragments arranged by size:
3413 2171 907 799 753 500 168 96
PasI CC'CwG_GG
Cuts at: 1128 1495 2904 3311 4455 4700 5148 5196 1128
Size: 367 1409 407 1144 245 448 48 4739
Fragments arranged by size:
4739 1409 1144 448 407 367 245 48
PciI A'CATG_T
Cuts at: 4040 7263 4040
Size: 3223 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1174 3866 4521 4706 1174
Size: 2692 655 185 5275
Fragments arranged by size:
5275 2692 655 185
PfoI T'CCnGG_A
Cuts at: 401 662 828 3794 3814 401
Size: 261 166 2966 20 5394
Fragments arranged by size:
5394 2966 261 166 20
PmeI GTTT'AAAC
Cuts at: 5806 5806
Size: 8807
PmlI CAC'GTG
Cuts at: 3612 3612
Size: 8807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7946 7978 7946
Size: 32 8775
PpuMI rG'GwC_Cy
Cuts at: 636 851 911 1639 2023 2278 5226 5385 5601
Size: 215 60 728 384 255 2948 159 216
Cuts at: 5601 5650 636
Size: 49 3793
Fragments arranged by size:
3793 2948 728 384 255 216 215 159
60 49
PspOMI G'GGCC_C
Cuts at: 277 386 537 599 867 1299 2357 3049 3257
Size: 109 151 62 268 432 1058 692 208
Cuts at: 3257 3453 3708 3749 3911 4769 4974 5152 5279
Size: 196 255 41 162 858 205 178 127
Cuts at: 5279 277
Size: 3805
Fragments arranged by size:
3805 1058 858 692 432 268 255 208
205 196 178 162 151 127 109 62
41
PspXI vC'TCGA_Gb
Cuts at: 271 271
Size: 8807
PstI C_TGCA'G
Cuts at: 647 1442 1758 1806 1860 2022 2058 2082 2100
Size: 795 316 48 54 162 36 24 18
Cuts at: 2100 2154 2534 4595 4858 4961 5854 6539 647
Size: 54 380 2061 263 103 893 685 2915
Fragments arranged by size:
2915 2061 893 795 685 380 316 263
162 103 54 54 48 36 24 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8807
PvuII CAG'CTG
Cuts at: 648 957 1399 2628 3579 4554 5484 6232 6592
Size: 309 442 1229 951 975 930 748 360
Cuts at: 6592 648
Size: 2863
Fragments arranged by size:
2863 1229 975 951 930 748 442 360
309
RsrII CG'GwC_CG
Cuts at: 7002 7002
Size: 8807
SacI G_AGCT'C
Cuts at: 1504 1719 2139 4271 5254 5507 5821 1504
Size: 215 420 2132 983 253 314 4490
Fragments arranged by size:
4490 2132 983 420 314 253 215
SacII CC_GC'GG
Cuts at: 3971 3971
Size: 8807
SalI G'TCGA_C
Cuts at: 4935 5844 4935
Size: 909 7898
SanDI GG'GwC_CC
Cuts at: 851 911 1639 2278 5650 851
Size: 60 728 639 3372 4008
Fragments arranged by size:
4008 3372 728 639 60
SapI GCTCTTCn'nnn_
Cuts at: 6830 7040 6830
Size: 210 8597
SbfI CC_TGCA'GG
Cuts at: 2022 5854 2022
Size: 3832 4975
ScaI AGT'ACT
Cuts at: 8324 8324
Size: 8807
SexAI A'CCwGG_T
Cuts at: 434 5035 5597 434
Size: 4601 562 3644
Fragments arranged by size:
4601 3644 562
SfcI C'TryA_G
Cuts at: 33 265 643 1438 1573 1754 1802 1856 2000
Size: 232 378 795 135 181 48 54 144
Cuts at: 2000 2018 2054 2060 2078 2096 2150 2530 4502
Size: 18 36 6 18 18 54 380 1972
Cuts at: 4502 4591 4854 4957 5479 5688 5850 6535 7528
Size: 89 263 103 522 209 162 685 993
Cuts at: 7528 7719 33
Size: 191 1121
Fragments arranged by size:
1972 1121 993 795 685 522 380 378
263 232 209 191 181 162 144 135
103 89 54 54 48 36 18 18
18 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 598 1185 2915 3523 3622 5353 598
Size: 587 1730 608 99 1731 4052
Fragments arranged by size:
4052 1731 1730 608 587 99
SfoI GGC'GCC
Cuts at: 6239 6487 6239
Size: 248 8559
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8807
SmaI CCC'GGG
Cuts at: 562 589 1163 5829 562
Size: 27 574 4666 3540
Fragments arranged by size:
4666 3540 574 27
SmlI C'TyrA_G
Cuts at: 271 1001 1682 1790 1952 2042 2683 2713 3472
Size: 730 681 108 162 90 641 30 759
Cuts at: 3472 3754 5284 5458 5962 6877 7369 7631 271
Size: 282 1530 174 504 915 492 262 1447
Fragments arranged by size:
1530 1447 915 759 730 681 641 504
492 282 262 174 162 108 90 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8807
SphI G_CATG'C
Cuts at: 631 5860 6360 631
Size: 5229 500 3078
Fragments arranged by size:
5229 3078 500
StuI AGG'CCT
Cuts at: 395 1153 3517 5755 395
Size: 758 2364 2238 3447
Fragments arranged by size:
3447 2364 2238 758
StyI C'CwwG_G
Cuts at: 302 381 390 806 1450 1748 2186 2297 2362
Size: 79 9 416 644 298 438 111 65
Cuts at: 2362 2516 2561 2924 4520 4763 4921 5941 6295
Size: 154 45 363 1596 243 158 1020 354
Cuts at: 6295 302
Size: 2814
Fragments arranged by size:
2814 1596 1020 644 438 416 363 354
298 243 158 154 111 79 65 45
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1120 2049 4343 5031 5581 5634 6390 6523 6982
Size: 929 2294 688 550 53 756 133 459
Cuts at: 6982 7191 1120
Size: 209 2736
Fragments arranged by size:
2736 2294 929 756 688 550 459 209
133 53
TatI w'GTAC_w
Cuts at: 5010 8322 5010
Size: 3312 5495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 603 635 824 856 2527 2559 4060 4092 5192
Size: 32 189 32 1671 32 1501 32 1100
Cuts at: 5192 5224 5407 5439 5566 5577 5598 5609 8324
Size: 32 183 32 127 11 21 11 2715
Cuts at: 8324 8356 603
Size: 32 1054
Fragments arranged by size:
2715 1671 1501 1100 1054 189 183 127
32 32 32 32 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 480 5599 6604 480
Size: 5119 1005 2683
Fragments arranged by size:
5119 2683 1005
XbaI T'CTAG_A
Cuts at: 5838 5838
Size: 8807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 813 1576 2263 2295 2716 2835 5036 6302 813
Size: 763 687 32 421 119 2201 1266 3318
Fragments arranged by size:
3318 2201 1266 763 687 421 119 32
XhoI C'TCGA_G
Cuts at: 271 271
Size: 8807
XmaI C'CCGG_G
Cuts at: 560 587 1161 5827 560
Size: 27 574 4666 3540
Fragments arranged by size:
4666 3540 574 27
XmnI GAAnn'nnTTC
Cuts at: 229 2881 229
Size: 2652 6155
ZraI GAC'GTC
Cuts at: 5469 5469
Size: 8807
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BfrBI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PsrI SgrAI SnaBI SrfI SspI SwaI