Restriction map for
pF1KA1301
(Circular) (Six-base) MAPSORT of: pF1KA1301.seq Check: 4850 from: 1 to: 7829
pF1KA1301 7829 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 140 2631 140
Size: 2491 5338
AatII G_ACGT'C
Cuts at: 1344 1344
Size: 7829
AccI GT'mk_AC
Cuts at: 805 3177 4007 4867 805
Size: 2372 830 860 3767
Fragments arranged by size:
3767 2372 860 830
Acc65I G'GTAC_C
Cuts at: 1049 2510 3637 4845 1049
Size: 1461 1127 1208 4033
Fragments arranged by size:
4033 1461 1208 1127
AclI AA'CG_TT
Cuts at: 7669 7669
Size: 7829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 2471 3151 3488 5654 6086 6833 7101 617
Size: 1854 680 337 2166 432 747 268 1345
Fragments arranged by size:
2166 1854 1345 747 680 432 337 268
AflII C'TTAA_G
Cuts at: 733 733
Size: 7829
AflIII A'CryG_T
Cuts at: 2948 2950 3041 4723 5181 6285 7262 2948
Size: 2 91 1682 458 1104 977 3515
Fragments arranged by size:
3515 1682 1104 977 458 91 2
AgeI A'CCGG_T
Cuts at: 6217 6217
Size: 7829
AleI CACnn'nnGTG
Cuts at: 1043 1043
Size: 7829
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 7797
AlwNI CAG_nnn'CTG
Cuts at: 969 1108 1225 2039 2353 2472 2646 3587 4176
Size: 139 117 814 314 119 174 941 589
Cuts at: 4176 5134 6701 969
Size: 958 1567 2097
Fragments arranged by size:
2097 1567 958 941 814 589 314 174
139 119 117
ApaI G_GGCC'C
Cuts at: 494 494
Size: 7829
ApaLI G'TGCA_C
Cuts at: 2211 6599 2211
Size: 4388 3441
ApoI r'AATT_y
Cuts at: 405 904 3395 4121 4833 7182 7438 405
Size: 499 2491 726 712 2349 256 796
Fragments arranged by size:
2491 2349 796 726 712 499 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7829
AvaI C'yCGr_G
Cuts at: 122 3656 4849 122
Size: 3534 1193 3102
Fragments arranged by size:
3534 3102 1193
BamHI G'GATC_C
Cuts at: 4043 4854 4043
Size: 811 7018
BanI G'GyrC_C
Cuts at: 839 1049 1432 2241 2401 2510 3637 4282 4845
Size: 210 383 809 160 109 1127 645 563
Cuts at: 4845 5259 5507 5542 839
Size: 414 248 35 3126
Fragments arranged by size:
3126 1127 809 645 563 414 383 248
210 160 109 35
BanII G_rGCy'C
Cuts at: 181 214 494 555 1726 1921 2171 2365 3663
Size: 33 280 61 1171 195 250 194 1298
Cuts at: 3663 4843 5873 181
Size: 1180 1030 2137
Fragments arranged by size:
2137 1298 1180 1171 1030 280 250 195
194 61 33
BbeI G_GCGC'C
Cuts at: 5263 5511 5263
Size: 248 7581
BbsI GAAGACnn'nnnn_
Cuts at: 1070 1307 2153 1070
Size: 237 846 6746
Fragments arranged by size:
6746 846 237
BciVI GTATCCnnnnn_n'
Cuts at: 2713 3924 4510 5727 6488 7829 2713
Size: 1211 586 1217 761 1341 2713
Fragments arranged by size:
2713 1341 1217 1211 761 586
BclI T'GATC_A
Cuts at: 993 1571 2966 3093 5349 993
Size: 578 1395 127 2256 3473
Fragments arranged by size:
3473 2256 1395 578 127
BfrBI ATG'CAT
Cuts at: 1867 1867
Size: 7829
BglII A'GATC_T
Cuts at: 678 2653 5344 7381 678
Size: 1975 2691 2037 1126
Fragments arranged by size:
2691 2037 1975 1126
BlpI GC'TnA_GC
Cuts at: 1003 3523 4941 1003
Size: 2520 1418 3891
Fragments arranged by size:
3891 2520 1418
Bme1580I G_kGCm'C
Cuts at: 494 783 2215 2246 5454 5547 6603 494
Size: 289 1432 31 3208 93 1056 1720
Fragments arranged by size:
3208 1720 1432 1056 289 93 31
BmgBI CAC'GTC
Cuts at: 2609 2609
Size: 7829
BmrI ACTGGGnnnn_n'
Cuts at: 365 1775 1949 2240 2553 4288 5041 5122 5457
Size: 1410 174 291 313 1735 753 81 335
Cuts at: 5457 7601 365
Size: 2144 593
Fragments arranged by size:
2144 1735 1410 753 593 335 313 291
174 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 297 1677 6196 297
Size: 1380 4519 1930
Fragments arranged by size:
4519 1930 1380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 267 395 994 1039 1085 1377 1651 2356
Size: 92 128 599 45 46 292 274 705
Cuts at: 2356 2462 2817 3461 3647 3801 175
Size: 106 355 644 186 154 4203
Fragments arranged by size:
4203 705 644 599 355 292 274 186
154 128 106 92 46 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1802 2116 2435 4840 5005 5884 6376 6674 1802
Size: 314 319 2405 165 879 492 298 2957
Fragments arranged by size:
2957 2405 879 492 319 314 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 1891 7533 1891
Size: 5642 2187
BsaAI yAC'GTr
Cuts at: 1041 2583 2592 2951 3601 5182 1041
Size: 1542 9 359 650 1581 3688
Fragments arranged by size:
3688 1581 1542 650 359 9
BsaBI GATnn'nnATC
Cuts at: 6 517 5343 7380 6
Size: 511 4826 2037 455
Fragments arranged by size:
4826 2037 511 455
BsaHI Gr'CG_yC
Cuts at: 147 1341 5260 5508 6210 7706 147
Size: 1194 3919 248 702 1496 270
Fragments arranged by size:
3919 1496 1194 702 270 248
BsaWI w'CCGG_w
Cuts at: 507 2978 5018 5539 6217 6491 6638 7049 507
Size: 2471 2040 521 678 274 147 411 1287
Fragments arranged by size:
2471 2040 1287 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 787 817 1060 1090 1314 1344 2815 2845 3112
Size: 30 243 30 224 30 1471 30 267
Cuts at: 3112 3142 787
Size: 30 5474
Fragments arranged by size:
5474 1471 267 243 224 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 1464 1570 1573 2079 2155 2314 2443 3611
Size: 1091 106 3 506 76 159 129 1168
Cuts at: 3611 4526 373
Size: 915 3676
Fragments arranged by size:
3676 1168 1091 915 506 159 129 106
76 3
BseYI C'CCAG_C
Cuts at: 315 591 1029 1740 2121 2304 2386 2631 3580
Size: 276 438 711 381 183 82 245 949
Cuts at: 3580 3641 5254 6012 6589 7085 315
Size: 61 1613 758 577 496 1059
Fragments arranged by size:
1613 1059 949 758 711 577 496 438
381 276 245 183 82 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2813 2180
Size: 633 7196
BsiEI CG_ry'CG
Cuts at: 104 2620 5371 5417 6625 104
Size: 2516 2751 46 1208 1308
Fragments arranged by size:
2751 2516 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 214 1210 1726 2089 2215 4843 5621 5811
Size: 83 996 516 363 126 2628 778 190
Cuts at: 5811 6603 131
Size: 792 1357
Fragments arranged by size:
2628 1357 996 792 778 516 363 190
126 83
BsmI GAATG_Cn'
Cuts at: 645 1257 2029 2910 3109 4622 645
Size: 612 772 881 199 1513 3852
Fragments arranged by size:
3852 1513 881 772 612 199
BsmBI CGTCTCn'nnnn_
Cuts at: 792 792
Size: 7829
Bsp1286I G_dGCh'C
Cuts at: 131 181 214 494 555 783 1210 1726 1921
Size: 50 33 280 61 228 427 516 195
Cuts at: 1921 2089 2171 2215 2246 2365 3663 4843 5454
Size: 168 82 44 31 119 1298 1180 611
Cuts at: 5454 5547 5621 5811 5873 6603 131
Size: 93 74 190 62 730 1357
Fragments arranged by size:
1357 1298 1180 730 611 516 427 280
228 195 190 168 119 93 82 74
62 61 50 44 33 31
BspEI T'CCGG_A
Cuts at: 2978 7049 2978
Size: 4071 3758
BspHI T'CATG_A
Cuts at: 1086 7005 1086
Size: 5919 1910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 140 650 1420 1740 2631 3836 4879 5093 5395
Size: 510 770 320 891 1205 1043 214 302
Cuts at: 5395 5776 140
Size: 381 2193
Fragments arranged by size:
2193 1205 1043 891 770 510 381 320
302 214
BsrBI CCG'CTC
Cuts at: 50 281 6121 6175 7659 7824 50
Size: 231 5840 54 1484 165 55
Fragments arranged by size:
5840 1484 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2049 5741 2049
Size: 3692 4137
BsrFI r'CCGG_y
Cuts at: 2507 2687 5827 6008 6217 2507
Size: 180 3140 181 209 4119
Fragments arranged by size:
4119 3140 209 181 180
BsrGI T'GTAC_A
Cuts at: 4362 4362
Size: 7829
BssSI C'ACGA_G
Cuts at: 1516 6100 6458 1516
Size: 4584 358 2887
Fragments arranged by size:
4584 2887 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2353 2646 5206 2353
Size: 293 2560 4976
Fragments arranged by size:
4976 2560 293
BstBI TT'CG_AA
Cuts at: 3686 6190 3686
Size: 2504 5325
BstEII G'GTnAC_C
Cuts at: 1537 1537
Size: 7829
BstXI CCAn_nnnn'nTGG
Cuts at: 1045 2543 1045
Size: 1498 6331
BstYI r'GATC_y
Cuts at: 1 678 2573 2653 2836 3449 4043 4740 4854
Size: 677 1895 80 183 613 594 697 114
Cuts at: 4854 5321 5344 5679 5925 6937 7023 7035 7381
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7381 1
Size: 449
Fragments arranged by size:
1895 1012 697 677 613 594 467 449
346 335 246 183 114 86 80 23
12
Bsu36I CC'TnA_GG
Cuts at: 2003 2732 2003
Size: 729 7100
BtgI C'CryG_G
Cuts at: 107 537 786 1427 2565 3070 107
Size: 430 249 641 1138 505 4866
Fragments arranged by size:
4866 1138 641 505 430 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3515 4440 5779 5960 6123 3515
Size: 925 1339 181 163 5221
Fragments arranged by size:
5221 1339 925 181 163
BtsI GCAGTG_nn'
Cuts at: 1803 2184 2213 2388 2470 4186 4565 4700 5072
Size: 381 29 175 82 1716 379 135 372
Cuts at: 5072 1803
Size: 4560
Fragments arranged by size:
4560 1716 381 379 372 175 135 82
29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3129 3164 3129
Size: 35 7794
DraI TTT'AAA
Cuts at: 528 1062 4828 528
Size: 534 3766 3529
Fragments arranged by size:
3766 3529 534
DraIII CAC_nnn'GTG
Cuts at: 2084 2609 2084
Size: 525 7304
DrdI GACnn_nn'nnGTC
Cuts at: 5535 6393 5535
Size: 858 6971
EaeI y'GGCC_r
Cuts at: 1386 2279 2460 5414 5588 5979 6006 7351 1386
Size: 893 181 2954 174 391 27 1345 1864
Fragments arranged by size:
2954 1864 1345 893 391 181 174 27
EagI C'GGCC_G
Cuts at: 5414 5414
Size: 7829
EarI CTCTTCn'nnn_
Cuts at: 754 1878 3533 3765 4118 4154 4712 5852 6062
Size: 1124 1655 232 353 36 558 1140 210
Cuts at: 6062 754
Size: 2521
Fragments arranged by size:
2521 1655 1140 1124 558 353 232 210
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6037 6347 6493 7639 6037
Size: 310 146 1146 6227
Fragments arranged by size:
6227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 212 1724 4841 212
Size: 1512 3117 3200
Fragments arranged by size:
3200 3117 1512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 267 395 617 994 1039 1085 1377 1651
Size: 92 128 222 377 45 46 292 274
Cuts at: 1651 2356 2462 2471 2817 3151 3461 3488 3647
Size: 705 106 9 346 334 310 27 159
Cuts at: 3647 3801 5654 6086 6833 7101 175
Size: 154 1853 432 747 268 903
Fragments arranged by size:
1853 903 747 705 432 377 346 334
310 292 274 268 222 159 154 128
106 92 46 45 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1334 2189 1334
Size: 855 6974
EcoO109I rG'GnC_Cy
Cuts at: 491 1322 4968 491
Size: 831 3646 3352
Fragments arranged by size:
3646 3352 831
EcoRI G'AATT_C
Cuts at: 405 4121 4833 405
Size: 3716 712 3401
Fragments arranged by size:
3716 3401 712
EcoRV GAT'ATC
Cuts at: 2695 4200 2695
Size: 1505 6324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3129 5848 3129
Size: 2719 5110
FspI TGC'GCA
Cuts at: 5610 5610
Size: 7829
HaeII r_GCGC'y
Cuts at: 2438 5263 5511 6533 7512 2438
Size: 2825 248 1022 979 2755
Fragments arranged by size:
2825 2755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 971 1003 1060 1092 1314 1346 1556
Size: 32 152 32 57 32 222 32 210
Cuts at: 1556 1588 1888 1890 1903 1920 1922 1935 2828
Size: 32 300 2 13 17 2 13 893
Cuts at: 2828 2860 3112 3144 3532 3564 4035 4067 5671
Size: 32 252 32 388 32 471 32 1604
Cuts at: 5671 5701 5703 5733 787
Size: 30 2 30 2883
Fragments arranged by size:
2883 1604 893 471 388 300 252 222
210 152 57 32 32 32 32 32
32 32 32 32 30 30 17 13
13 2 2 2
HincII GTy'rAC
Cuts at: 3379 3412 4787 4868 3379
Size: 33 1375 81 6340
Fragments arranged by size:
6340 1375 81 33
KasI G'GCGC_C
Cuts at: 5259 5507 5259
Size: 248 7581
KpnI G_GTAC'C
Cuts at: 1053 2514 3641 4849 1053
Size: 1461 1127 1208 4033
Fragments arranged by size:
4033 1461 1208 1127
MluI A'CGCG_T
Cuts at: 7262 7262
Size: 7829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2826 4058 6500 6684 2826
Size: 1232 2442 184 3971
Fragments arranged by size:
3971 2442 1232 184
MscI TGG'CCA
Cuts at: 1388 5590 1388
Size: 4202 3627
MslI CAynn'nnrTG
Cuts at: 398 580 1043 1091 5818 5945 398
Size: 182 463 48 4727 127 2282
Fragments arranged by size:
4727 2282 463 182 127 48
MspA1I CmG'CkG
Cuts at: 240 595 1033 2064 2322 2350 2682 3072 4468
Size: 355 438 1031 258 28 332 390 1396
Cuts at: 4468 4940 5254 5614 6627 6872 240
Size: 472 314 360 1013 245 1197
Fragments arranged by size:
1396 1197 1031 1013 472 438 390 360
355 332 314 258 245 28
NaeI GCC'GGC
Cuts at: 6010 6010
Size: 7829
NarI GG'CG_CC
Cuts at: 5260 5508 5260
Size: 248 7581
NcoI C'CATG_G
Cuts at: 107 1427 107
Size: 1320 6509
NdeI CA'TA_TG
Cuts at: 3552 3552
Size: 7829
NgoMIV G'CCGG_C
Cuts at: 6008 6008
Size: 7829
NruI TCG'CGA
Cuts at: 3049 3049
Size: 7829
NsiI A_TGCA'T
Cuts at: 1869 1869
Size: 7829
NspI r_CATG'y
Cuts at: 303 1179 2937 3045 3265 3568 4727 4882 5382
Size: 876 1758 108 220 303 1159 155 500
Cuts at: 5382 6289 303
Size: 907 1843
Fragments arranged by size:
1843 1758 1159 907 876 500 303 220
155 108
PasI CC'CwG_GG
Cuts at: 3745 3745
Size: 7829
PciI A'CATG_T
Cuts at: 3041 4723 6285 3041
Size: 1682 1562 4585
Fragments arranged by size:
4585 1682 1562
PflMI CCAn_nnn'nTGG
Cuts at: 377 422 2520 2609 3333 3940 377
Size: 45 2098 89 724 607 4266
Fragments arranged by size:
4266 2098 724 607 89 45
PfoI T'CCnGG_A
Cuts at: 2839 3477 2839
Size: 638 7191
PmeI GTTT'AAAC
Cuts at: 4828 4828
Size: 7829
PmlI CAC'GTG
Cuts at: 1041 2951 3601 1041
Size: 1910 650 5269
Fragments arranged by size:
5269 1910 650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6968 7000 6968
Size: 32 7797
PpuMI rG'GwC_Cy
Cuts at: 1322 1322
Size: 7829
PsiI TTA'TAA
Cuts at: 4106 4106
Size: 7829
PspOMI G'GGCC_C
Cuts at: 490 490
Size: 7829
PstI C_TGCA'G
Cuts at: 239 1737 2349 2651 3833 4181 4560 4876 5561
Size: 1498 612 302 1182 348 379 316 685
Cuts at: 5561 239
Size: 2507
Fragments arranged by size:
2507 1498 1182 685 612 379 348 316
302
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7829
PvuII CAG'CTG
Cuts at: 595 1033 2064 2350 2682 4468 5254 5614 595
Size: 438 1031 286 332 1786 786 360 2810
Fragments arranged by size:
2810 1786 1031 786 438 360 332 286
RsrII CG'GwC_CG
Cuts at: 6024 6024
Size: 7829
SacI G_AGCT'C
Cuts at: 214 1726 4843 214
Size: 1512 3117 3200
Fragments arranged by size:
3200 3117 1512
SacII CC_GC'GG
Cuts at: 3073 3073
Size: 7829
SalI G'TCGA_C
Cuts at: 4866 4866
Size: 7829
SapI GCTCTTCn'nnn_
Cuts at: 754 1878 4118 4712 5852 6062 754
Size: 1124 2240 594 1140 210 2521
Fragments arranged by size:
2521 2240 1140 1124 594 210
SbfI CC_TGCA'GG
Cuts at: 1737 4876 1737
Size: 3139 4690
ScaI AGT'ACT
Cuts at: 4809 7346 4809
Size: 2537 5292
SexAI A'CCwGG_T
Cuts at: 260 260
Size: 7829
SfcI C'TryA_G
Cuts at: 33 235 1733 2345 2647 3400 3829 4177 4556
Size: 202 1498 612 302 753 429 348 379
Cuts at: 4556 4872 5557 6550 6741 33
Size: 316 685 993 191 1121
Fragments arranged by size:
1498 1121 993 753 685 612 429 379
348 316 302 202 191
SfoI GGC'GCC
Cuts at: 5261 5509 5261
Size: 248 7581
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7829
SmaI CCC'GGG
Cuts at: 124 3658 4851 124
Size: 3534 1193 3102
Fragments arranged by size:
3534 3102 1193
SmlI C'TyrA_G
Cuts at: 733 1817 2095 2450 4819 4984 5899 6391 6653
Size: 1084 278 355 2369 165 915 492 262
Cuts at: 6653 733
Size: 1909
Fragments arranged by size:
2369 1909 1084 915 492 355 278 262
165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7829
SphI G_CATG'C
Cuts at: 4882 5382 4882
Size: 500 7329
SrfI GCCC'GGGC
Cuts at: 3658 3658
Size: 7829
SspI AAT'ATT
Cuts at: 532 853 3955 532
Size: 321 3102 4406
Fragments arranged by size:
4406 3102 321
StuI AGG'CCT
Cuts at: 1568 2001 2730 1568
Size: 433 729 6667
Fragments arranged by size:
6667 729 433
StyI C'CwwG_G
Cuts at: 107 1014 1427 1550 2397 4047 4963 5317 107
Size: 907 413 123 847 1650 916 354 2619
Fragments arranged by size:
2619 1650 916 907 847 413 354 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1420 1915 2634 3004 5412 5545 6004 6213 1420
Size: 495 719 370 2408 133 459 209 3036
Fragments arranged by size:
3036 2408 719 495 459 370 209 133
TatI w'GTAC_w
Cuts at: 308 3295 3978 4319 4362 4807 7344 308
Size: 2987 683 341 43 445 2537 793
Fragments arranged by size:
2987 2537 793 683 445 341 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 813 845 1962 1994 2068 2100 2194 2226 2815
Size: 32 1117 32 74 32 94 32 589
Cuts at: 2815 2847 4692 4724 7346 7378 813
Size: 32 1845 32 2622 32 1264
Fragments arranged by size:
2622 1845 1264 1117 589 94 74 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1902 5626 1902
Size: 3724 4105
XbaI T'CTAG_A
Cuts at: 3892 4860 3892
Size: 968 6861
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 419 544 2670 3189 5324 419
Size: 125 2126 519 2135 2924
Fragments arranged by size:
2924 2135 2126 519 125
XmaI C'CCGG_G
Cuts at: 122 3656 4849 122
Size: 3534 1193 3102
Fragments arranged by size:
3534 3102 1193
XmnI GAAnn'nnTTC
Cuts at: 1703 1703
Size: 7829
ZraI GAC'GTC
Cuts at: 1342 1342
Size: 7829
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AscI AvrII BaeI BbvCI BcgI
BglI BmtI Bpu10I BsiWI BssHII BstZ17I ClaI FseI
FspAI HindIII HpaI MfeI NheI NotI PacI PshAI
PspXI PsrI SanDI SfiI SgrAI SnaBI SwaI XhoI