Restriction map for
pF1KA1291
(Circular) (Six-base) MAPSORT of: pF1KA1291.seq Check: 7587 from: 1 to: 6725
pF1KA1291 6725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2282 3763 2282
Size: 1481 5244
Acc65I G'GTAC_C
Cuts at: 3428 3741 3428
Size: 313 6412
AclI AA'CG_TT
Cuts at: 6565 6565
Size: 6725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 397 660 880 1084 1227 4550 4982 5729
Size: 102 263 220 204 143 3323 432 747
Cuts at: 5729 5997 295
Size: 268 1023
Fragments arranged by size:
3323 1023 747 432 268 263 220 204
143 102
AfeI AGC'GCT
Cuts at: 190 3188 190
Size: 2998 3727
AflIII A'CryG_T
Cuts at: 324 4077 5181 6158 324
Size: 3753 1104 977 891
Fragments arranged by size:
3753 1104 977 891
AgeI A'CCGG_T
Cuts at: 5113 5113
Size: 6725
AhdI GACnn_n'nnGTC
Cuts at: 819 819
Size: 6725
AleI CACnn'nnGTG
Cuts at: 3065 3331 3065
Size: 266 6459
AlwNI CAG_nnn'CTG
Cuts at: 318 1129 2144 2415 4030 5597 318
Size: 811 1015 271 1615 1567 1446
Fragments arranged by size:
1615 1567 1446 1015 811 271
ApaLI G'TGCA_C
Cuts at: 2261 5495 2261
Size: 3234 3491
ApoI r'AATT_y
Cuts at: 1545 1682 3729 6078 6334 1545
Size: 137 2047 2349 256 1936
Fragments arranged by size:
2349 2047 1936 256 137
AseI AT'TA_AT
Cuts at: 20 3193 20
Size: 3173 3552
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6725
AvaI C'yCGr_G
Cuts at: 1424 1889 3005 3745 1424
Size: 465 1116 740 4404
Fragments arranged by size:
4404 1116 740 465
AvrII C'CTAG_G
Cuts at: 2658 2658
Size: 6725
BamHI G'GATC_C
Cuts at: 1467 1740 2241 3750 1467
Size: 273 501 1509 4442
Fragments arranged by size:
4442 1509 501 273
BanI G'GyrC_C
Cuts at: 2127 3428 3741 4155 4403 4438 2127
Size: 1301 313 414 248 35 4414
Fragments arranged by size:
4414 1301 414 313 248 35
BanII G_rGCy'C
Cuts at: 1239 3739 4769 1239
Size: 2500 1030 3195
Fragments arranged by size:
3195 2500 1030
BbeI G_GCGC'C
Cuts at: 4159 4407 4159
Size: 248 6477
BbsI GAAGACnn'nnnn_
Cuts at: 736 910 959 1584 1818 2583 736
Size: 174 49 625 234 765 4878
Fragments arranged by size:
4878 765 625 234 174 49
BbvCI CC'TCA_GC
Cuts at: 3123 3123
Size: 6725
BciVI GTATCCnnnnn_n'
Cuts at: 2853 4623 5384 6725 2853
Size: 1770 761 1341 2853
Fragments arranged by size:
2853 1770 1341 761
BclI T'GATC_A
Cuts at: 476 4245 476
Size: 3769 2956
BfrBI ATG'CAT
Cuts at: 996 996
Size: 6725
BglI GCCn_nnn'nGGC
Cuts at: 2491 2491
Size: 6725
BglII A'GATC_T
Cuts at: 4240 6277 4240
Size: 2037 4688
BlpI GC'TnA_GC
Cuts at: 2730 3837 2730
Size: 1107 5618
Bme1580I G_kGCm'C
Cuts at: 2049 2265 4350 4443 5499 2049
Size: 216 2085 93 1056 3275
Fragments arranged by size:
3275 2085 1056 216 93
BmrI ACTGGGnnnn_n'
Cuts at: 825 3937 4018 4353 6497 825
Size: 3112 81 335 2144 1053
Fragments arranged by size:
3112 2144 1053 335 81
BmtI G_CTAG'C
Cuts at: 2485 2485
Size: 6725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 5092 2906
Size: 2186 4539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1589 2257 2998 3454 3685 880
Size: 709 668 741 456 231 3920
Fragments arranged by size:
3920 741 709 668 456 231
Bpu10I CC'TnA_GC
Cuts at: 3123 3123
Size: 6725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 282 2138 2951 3901 4780 5272 5570 193
Size: 89 1856 813 950 879 492 298 1348
Fragments arranged by size:
1856 1348 950 879 813 492 298 89
BsaI GGTCTCn'nnnn_
Cuts at: 6429 6429
Size: 6725
BsaAI yAC'GTr
Cuts at: 3317 4078 3317
Size: 761 5964
BsaBI GATnn'nnATC
Cuts at: 6 571 4239 6276 6
Size: 565 3668 2037 455
Fragments arranged by size:
3668 2037 565 455
BsaHI Gr'CG_yC
Cuts at: 4156 4404 5106 6602 4156
Size: 248 702 1496 4279
Fragments arranged by size:
4279 1496 702 248
BsaWI w'CCGG_w
Cuts at: 956 3914 4435 5113 5387 5534 5945 956
Size: 2958 521 678 274 147 411 1736
Fragments arranged by size:
2958 1736 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 355 385 853 883 1025 1055 2230
Size: 30 162 30 468 30 142 30 1175
Cuts at: 2230 2260 163
Size: 30 4628
Fragments arranged by size:
4628 1175 468 162 142 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 445 1046 1410 1923 1996 2131 2995 3262
Size: 210 601 364 513 73 135 864 267
Cuts at: 3262 235
Size: 3698
Fragments arranged by size:
3698 864 601 513 364 267 210 135
73
BseYI C'CCAG_C
Cuts at: 185 1092 1116 1492 1955 2344 2717 3075 4150
Size: 907 24 376 463 389 373 358 1075
Cuts at: 4150 4908 5485 5981 185
Size: 758 577 496 929
Fragments arranged by size:
1075 929 907 758 577 496 463 389
376 373 358 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1142 1630 1763 2385 1142
Size: 488 133 622 5482
Fragments arranged by size:
5482 622 488 133
BsiEI CG_ry'CG
Cuts at: 104 4267 4313 5521 104
Size: 4163 46 1208 1308
Fragments arranged by size:
4163 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1431 2265 2810 2828 3072 3739 4517 4707 5499
Size: 834 545 18 244 667 778 190 792
Cuts at: 5499 1431
Size: 2657
Fragments arranged by size:
2657 834 792 778 667 545 244 190
18
BsmI GAATG_Cn'
Cuts at: 2288 2618 2288
Size: 330 6395
BsmBI CGTCTCn'nnnn_
Cuts at: 3080 3661 3080
Size: 581 6144
Bsp1286I G_dGCh'C
Cuts at: 1239 1431 2049 2265 2810 2828 3072 3739 4350
Size: 192 618 216 545 18 244 667 611
Cuts at: 4350 4443 4517 4707 4769 5499 1239
Size: 93 74 190 62 730 2465
Fragments arranged by size:
2465 730 667 618 611 545 244 216
192 190 93 74 62 18
BspEI T'CCGG_A
Cuts at: 5945 5945
Size: 6725
BspHI T'CATG_A
Cuts at: 164 5901 164
Size: 5737 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3775 3989 4291 4672 3775
Size: 214 302 381 5828
Fragments arranged by size:
5828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5017 5071 6555 6720 50
Size: 4967 54 1484 165 55
Fragments arranged by size:
4967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 923 1111 3566 4637 923
Size: 188 2455 1071 3011
Fragments arranged by size:
3011 2455 1071 188
BsrFI r'CCGG_y
Cuts at: 194 4723 4904 5113 194
Size: 4529 181 209 1806
Fragments arranged by size:
4529 1806 209 181
BsrGI T'GTAC_A
Cuts at: 3181 3181
Size: 6725
BssSI C'ACGA_G
Cuts at: 4996 5354 4996
Size: 358 6367
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 2169 4102 1021
Size: 1148 1933 3644
Fragments arranged by size:
3644 1933 1148
BstBI TT'CG_AA
Cuts at: 1662 5086 1662
Size: 3424 3301
BstEII G'GTnAC_C
Cuts at: 3114 3114
Size: 6725
BstXI CCAn_nnnn'nTGG
Cuts at: 114 500 1492 2514 3432 114
Size: 386 992 1022 918 3407
Fragments arranged by size:
3407 1022 992 918 386
BstYI r'GATC_y
Cuts at: 1 314 671 1467 1740 2241 3750 4217 4240
Size: 313 357 796 273 501 1509 467 23
Cuts at: 4240 4575 4821 5833 5919 5931 6277 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1509 1012 796 501 467 449 357 346
335 313 273 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2283 2283
Size: 6725
BtgI C'CryG_G
Cuts at: 107 107
Size: 6725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 1406 4675 4856 5019 127
Size: 1279 3269 181 163 1833
Fragments arranged by size:
3269 1833 1279 181 163
BtsI GCAGTG_nn'
Cuts at: 836 1618 1901 3295 3415 3968 836
Size: 782 283 1394 120 553 3593
Fragments arranged by size:
3593 1394 782 553 283 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3693 3728 3693
Size: 35 6690
DraI TTT'AAA
Cuts at: 228 3509 3724 228
Size: 3281 215 3229
Fragments arranged by size:
3281 3229 215
DraIII CAC_nnn'GTG
Cuts at: 2169 2169
Size: 6725
DrdI GACnn_nn'nnGTC
Cuts at: 2587 4431 5289 2587
Size: 1844 858 4023
Fragments arranged by size:
4023 1844 858
EaeI y'GGCC_r
Cuts at: 490 2135 3104 3701 4310 4484 4875 4902 6247
Size: 1645 969 597 609 174 391 27 1345
Cuts at: 6247 490
Size: 968
Fragments arranged by size:
1645 1345 969 968 609 597 391 174
27
EagI C'GGCC_G
Cuts at: 4310 4310
Size: 6725
EarI CTCTTCn'nnn_
Cuts at: 422 1060 1218 1246 2212 2327 2815 4748 4958
Size: 638 158 28 966 115 488 1933 210
Cuts at: 4958 422
Size: 2189
Fragments arranged by size:
2189 1933 966 638 488 210 158 115
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 999 4933 5243 5389 6535 999
Size: 3934 310 146 1146 1189
Fragments arranged by size:
3934 1189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3737 3737
Size: 6725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 397 660 880 1084 1227 1589 2257 2998
Size: 102 263 220 204 143 362 668 741
Cuts at: 2998 3454 3685 4550 4982 5729 5997 295
Size: 456 231 865 432 747 268 1023
Fragments arranged by size:
1023 865 747 741 668 456 432 362
268 263 231 220 204 143 102
EcoNI CCTnn'n_nnAGG
Cuts at: 1819 2332 2662 3240 1819
Size: 513 330 578 5304
Fragments arranged by size:
5304 578 513 330
EcoO109I rG'GnC_Cy
Cuts at: 1878 2671 2706 3864 1878
Size: 793 35 1158 4739
Fragments arranged by size:
4739 1158 793 35
EcoRI G'AATT_C
Cuts at: 1545 3729 1545
Size: 2184 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1350 4744 1350
Size: 3394 3331
FspI TGC'GCA
Cuts at: 4506 4506
Size: 6725
HaeII r_GCGC'y
Cuts at: 192 3190 3543 4159 4407 5429 6408 192
Size: 2998 353 616 248 1022 979 509
Fragments arranged by size:
2998 1022 979 616 509 353 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 439 471 805 837 1059 1091 3217
Size: 32 244 32 334 32 222 32 2126
Cuts at: 3217 3249 4567 4597 4599 4629 163
Size: 32 1318 30 2 30 2259
Fragments arranged by size:
2259 2126 1318 334 244 222 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2742 3002 3764 2742
Size: 260 762 5703
Fragments arranged by size:
5703 762 260
HindIII A'AGCT_T
Cuts at: 3502 3502
Size: 6725
HpaI GTT'AAC
Cuts at: 3002 3002
Size: 6725
KasI G'GCGC_C
Cuts at: 4155 4403 4155
Size: 248 6477
KpnI G_GTAC'C
Cuts at: 3432 3745 3432
Size: 313 6412
MluI A'CGCG_T
Cuts at: 6158 6158
Size: 6725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 1542 5396 5580 929
Size: 613 3854 184 2074
Fragments arranged by size:
3854 2074 613 184
MscI TGG'CCA
Cuts at: 492 2137 3106 3703 4486 492
Size: 1645 969 597 783 2731
Fragments arranged by size:
2731 1645 969 783 597
MslI CAynn'nnrTG
Cuts at: 112 1654 1936 3065 3173 3331 4714 4841 112
Size: 1542 282 1129 108 158 1383 127 1996
Fragments arranged by size:
1996 1542 1383 1129 282 158 127 108
MspA1I CmG'CkG
Cuts at: 144 910 1104 3836 4150 4510 5523 5768 144
Size: 766 194 2732 314 360 1013 245 1101
Fragments arranged by size:
2732 1101 1013 766 360 314 245 194
NaeI GCC'GGC
Cuts at: 4906 4906
Size: 6725
NarI GG'CG_CC
Cuts at: 4156 4404 4156
Size: 248 6477
NcoI C'CATG_G
Cuts at: 107 107
Size: 6725
NdeI CA'TA_TG
Cuts at: 2879 2879
Size: 6725
NgoMIV G'CCGG_C
Cuts at: 4904 4904
Size: 6725
NheI G'CTAG_C
Cuts at: 2481 2481
Size: 6725
NsiI A_TGCA'T
Cuts at: 998 998
Size: 6725
NspI r_CATG'y
Cuts at: 354 513 1460 1918 1983 2163 3778 4278 5185
Size: 159 947 458 65 180 1615 500 907
Cuts at: 5185 354
Size: 1894
Fragments arranged by size:
1894 1615 947 907 500 458 180 159
65
PciI A'CATG_T
Cuts at: 5181 5181
Size: 6725
PflMI CCAn_nnn'nTGG
Cuts at: 663 2144 3082 663
Size: 1481 938 4306
Fragments arranged by size:
4306 1481 938
PfoI T'CCnGG_A
Cuts at: 317 857 317
Size: 540 6185
PmeI GTTT'AAAC
Cuts at: 3724 3724
Size: 6725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5864 5896 5864
Size: 32 6693
PpuMI rG'GwC_Cy
Cuts at: 2706 2706
Size: 6725
PsiI TTA'TAA
Cuts at: 1988 2446 1988
Size: 458 6267
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2442 2474 2442
Size: 32 6693
PstI C_TGCA'G
Cuts at: 1560 2949 3524 3772 4457 1560
Size: 1389 575 248 685 3828
Fragments arranged by size:
3828 1389 685 575 248
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6725
PvuII CAG'CTG
Cuts at: 144 1104 4150 4510 144
Size: 960 3046 360 2359
Fragments arranged by size:
3046 2359 960 360
RsrII CG'GwC_CG
Cuts at: 4920 4920
Size: 6725
SacI G_AGCT'C
Cuts at: 3739 3739
Size: 6725
SalI G'TCGA_C
Cuts at: 3762 3762
Size: 6725
SapI GCTCTTCn'nnn_
Cuts at: 1218 2212 2815 4748 4958 1218
Size: 994 603 1933 210 2985
Fragments arranged by size:
2985 1933 994 603 210
SbfI CC_TGCA'GG
Cuts at: 3772 3772
Size: 6725
ScaI AGT'ACT
Cuts at: 6242 6242
Size: 6725
SfcI C'TryA_G
Cuts at: 33 1556 1800 2945 3520 3768 4453 5446 5637
Size: 1523 244 1145 575 248 685 993 191
Cuts at: 5637 33
Size: 1121
Fragments arranged by size:
1523 1145 1121 993 685 575 248 244
191
SfoI GGC'GCC
Cuts at: 4157 4405 4157
Size: 248 6477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6725
SmaI CCC'GGG
Cuts at: 1891 3747 1891
Size: 1856 4869
SmlI C'TyrA_G
Cuts at: 208 261 2153 2966 3880 4795 5287 5549 208
Size: 53 1892 813 914 915 492 262 1384
Fragments arranged by size:
1892 1384 915 914 813 492 262 53
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6725
SphI G_CATG'C
Cuts at: 1918 3778 4278 1918
Size: 1860 500 4365
Fragments arranged by size:
4365 1860 500
SspI AAT'ATT
Cuts at: 1369 2754 1369
Size: 1385 5340
StuI AGG'CCT
Cuts at: 2234 2234
Size: 6725
StyI C'CwwG_G
Cuts at: 107 1736 2507 2658 3590 3859 4213 107
Size: 1629 771 151 932 269 354 2619
Fragments arranged by size:
2619 1629 932 771 354 269 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 807 1108 1666 2284 4308 4441 4900 5109
Size: 608 301 558 618 2024 133 459 209
Cuts at: 5109 199
Size: 1815
Fragments arranged by size:
2024 1815 618 608 558 459 301 209
133
TatI w'GTAC_w
Cuts at: 3181 6240 3181
Size: 3059 3666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 853 885 1176 1208 2230 2262 2342 2374 6242
Size: 32 291 32 1022 32 80 32 3868
Cuts at: 6242 6274 853
Size: 32 1304
Fragments arranged by size:
3868 1304 1022 291 80 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4522 4522
Size: 6725
XbaI T'CTAG_A
Cuts at: 3756 3756
Size: 6725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1317 4220 1317
Size: 2903 3822
XmaI C'CCGG_G
Cuts at: 1889 3745 1889
Size: 1856 4869
XmnI GAAnn'nnTTC
Cuts at: 1172 1682 2107 1172
Size: 510 425 5790
Fragments arranged by size:
5790 510 425
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI BaeI
BcgI BmgBI BsiWI BssHII Bsu36I ClaI EcoRV FseI
FspAI MfeI NotI NruI PacI PasI PmlI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI