Restriction map for
pF1KA1271
(Circular) (Six-base) MAPSORT of: pF1KA1271.seq Check: 7827 from: 1 to: 4733
pF1KA1271 4733 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 250 480 721 984 1572 250
Size: 230 241 263 588 3411
Fragments arranged by size:
3411 588 263 241 230
AccI GT'mk_AC
Cuts at: 380 1391 1771 380
Size: 1011 380 3342
Fragments arranged by size:
3342 1011 380
Acc65I G'GTAC_C
Cuts at: 1749 1749
Size: 4733
AclI AA'CG_TT
Cuts at: 4573 4573
Size: 4733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 277 2558 2990 3343 4005 140
Size: 137 2281 432 353 662 868
Fragments arranged by size:
2281 868 662 432 353 137
AflIII A'CryG_T
Cuts at: 2085 3885 4166 2085
Size: 1800 281 2652
Fragments arranged by size:
2652 1800 281
AleI CACnn'nnGTG
Cuts at: 1219 1219
Size: 4733
AlwNI CAG_nnn'CTG
Cuts at: 795 904 1060 1542 2038 3476 795
Size: 109 156 482 496 1438 2052
Fragments arranged by size:
2052 1438 496 482 156 109
ApaI G_GGCC'C
Cuts at: 464 1460 464
Size: 996 3737
ApaLI G'TGCA_C
Cuts at: 3571 3571
Size: 4733
ApoI r'AATT_y
Cuts at: 573 1737 4086 4342 573
Size: 1164 2349 256 964
Fragments arranged by size:
2349 1164 964 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4733
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
AvaI C'yCGr_G
Cuts at: 1404 1753 1404
Size: 349 4384
BamHI G'GATC_C
Cuts at: 1508 1758 1508
Size: 250 4483
BanI G'GyrC_C
Cuts at: 552 859 972 1182 1294 1498 1623 1749 2163
Size: 307 113 210 112 204 125 126 414
Cuts at: 2163 2411 2446 552
Size: 248 35 2839
Fragments arranged by size:
2839 414 307 248 210 204 126 125
113 112 35
BanII G_rGCy'C
Cuts at: 464 1460 1653 1747 2777 464
Size: 996 193 94 1030 2420
Fragments arranged by size:
2420 1030 996 193 94
BbeI G_GCGC'C
Cuts at: 556 1298 2167 2415 556
Size: 742 869 248 2874
Fragments arranged by size:
2874 869 742 248
BbsI GAAGACnn'nnnn_
Cuts at: 128 128
Size: 4733
BbvCI CC'TCA_GC
Cuts at: 667 667
Size: 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1405 1439 90
Size: 34 1281 34 3384
Fragments arranged by size:
3384 1281 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2631 3687 4733 2631
Size: 1056 1046 2631
Fragments arranged by size:
2631 1056 1046
BclI T'GATC_A
Cuts at: 2253 2253
Size: 4733
BglII A'GATC_T
Cuts at: 2248 4285 2248
Size: 2037 2696
BlpI GC'TnA_GC
Cuts at: 598 1311 1845 598
Size: 713 534 3486
Fragments arranged by size:
3486 713 534
Bme1580I G_kGCm'C
Cuts at: 464 1076 1460 1501 2358 2451 3575 464
Size: 612 384 41 857 93 1124 1622
Fragments arranged by size:
1622 1124 857 612 384 93 41
BmrI ACTGGGnnnn_n'
Cuts at: 721 1945 2026 2361 4505 721
Size: 1224 81 335 2144 949
Fragments arranged by size:
2144 1224 949 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 3100 666
Size: 2434 2299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 661 666 726 942 1322 1564 1645 448
Size: 213 5 60 216 380 242 81 3536
Fragments arranged by size:
3536 380 242 216 213 81 60 5
Bpu10I CC'TnA_GC
Cuts at: 667 667
Size: 4733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1909 2788 3502 3800 1909
Size: 879 714 298 2842
Fragments arranged by size:
2842 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 212 538 1261 4437 212
Size: 326 723 3176 508
Fragments arranged by size:
3176 723 508 326
BsaAI yAC'GTr
Cuts at: 1515 2086 1515
Size: 571 4162
BsaBI GATnn'nnATC
Cuts at: 6 2247 4284 6
Size: 2241 2037 455
Fragments arranged by size:
2241 2037 455
BsaHI Gr'CG_yC
Cuts at: 553 1295 1719 2164 2412 3114 4610 553
Size: 742 424 445 248 702 1496 676
Fragments arranged by size:
1496 742 702 676 445 424 248
BsaWI w'CCGG_w
Cuts at: 1601 1922 2443 3121 3532 3679 3953 1601
Size: 321 521 678 411 147 274 2381
Fragments arranged by size:
2381 678 521 411 321 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 1468 1498 421
Size: 30 1017 30 3656
Fragments arranged by size:
3656 1017 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 639 861 864 1279 1489 639
Size: 222 3 415 210 3883
Fragments arranged by size:
3883 415 222 210 3
BseYI C'CCAG_C
Cuts at: 308 1053 1272 1528 2158 2916 3581 3989 308
Size: 745 219 256 630 758 665 408 1052
Fragments arranged by size:
1052 758 745 665 630 408 256 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 944 1130 1708 228
Size: 716 186 578 3253
Fragments arranged by size:
3253 716 578 186
BsiEI CG_ry'CG
Cuts at: 104 1602 2275 2321 3551 104
Size: 1498 673 46 1230 1286
Fragments arranged by size:
1498 1286 1230 673 46
BsiHKAI G_wGCw'C
Cuts at: 1214 1747 2525 2715 3575 1214
Size: 533 778 190 860 2372
Fragments arranged by size:
2372 860 778 533 190
Bsp1286I G_dGCh'C
Cuts at: 464 1076 1214 1460 1501 1653 1747 2358 2451
Size: 612 138 246 41 152 94 611 93
Cuts at: 2451 2525 2715 2777 3575 464
Size: 74 190 62 798 1622
Fragments arranged by size:
1622 798 612 611 246 190 152 138
94 93 74 62 41
BspHI T'CATG_A
Cuts at: 3165 3165
Size: 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 250 480 721 984 1572 1783 1997 2299
Size: 44 230 241 263 588 211 214 302
Cuts at: 2299 2680 206
Size: 381 2259
Fragments arranged by size:
2259 588 381 302 263 241 230 214
211 44
BsrBI CCG'CTC
Cuts at: 50 3025 3079 4563 4728 50
Size: 2975 54 1484 165 55
Fragments arranged by size:
2975 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 174 323 2645 174
Size: 149 2322 2262
Fragments arranged by size:
2322 2262 149
BsrFI r'CCGG_y
Cuts at: 1291 1712 2731 2912 1291
Size: 421 1019 181 3112
Fragments arranged by size:
3112 1019 421 181
BssSI C'ACGA_G
Cuts at: 3004 3712 3004
Size: 708 4025
BstAPI GCAn_nnn'nTGC
Cuts at: 164 588 904 2110 164
Size: 424 316 1206 2787
Fragments arranged by size:
2787 1206 424 316
BstBI TT'CG_AA
Cuts at: 3094 3094
Size: 4733
BstYI r'GATC_y
Cuts at: 1 1419 1508 1758 2225 2248 2583 2829 3135
Size: 1418 89 250 467 23 335 246 306
Cuts at: 3135 3147 3233 4285 1
Size: 12 86 1052 449
Fragments arranged by size:
1418 1052 467 449 335 306 250 246
89 86 23 12
BtgI C'CryG_G
Cuts at: 759 1464 759
Size: 705 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2683 2864 3027 2683
Size: 181 163 4389
Fragments arranged by size:
4389 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1976 1118
Size: 858 3875
DraI TTT'AAA
Cuts at: 1732 1732
Size: 4733
DrdI GACnn_nn'nnGTC
Cuts at: 2439 3783 2439
Size: 1344 3389
EaeI y'GGCC_r
Cuts at: 2318 2492 2883 2910 4255 2318
Size: 174 391 27 1345 2796
Fragments arranged by size:
2796 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2318 2318
Size: 4733
EarI CTCTTCn'nnn_
Cuts at: 286 2756 2966 286
Size: 2470 210 2053
Fragments arranged by size:
2470 2053 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2941 3683 3829 4543 2941
Size: 742 146 714 3131
Fragments arranged by size:
3131 742 714 146
EcoICRI GAG'CTC
Cuts at: 1745 1745
Size: 4733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 277 448 661 666 726 942 1322 1564
Size: 137 171 213 5 60 216 380 242
Cuts at: 1564 1645 2558 2990 3343 4005 140
Size: 81 913 432 353 662 868
Fragments arranged by size:
913 868 662 432 380 353 242 216
213 171 137 81 60 5
EcoO109I rG'GnC_Cy
Cuts at: 460 461 1344 1456 1457 1637 1872 460
Size: 1 883 112 1 180 235 3321
Fragments arranged by size:
3321 883 235 180 112 1 1
EcoRI G'AATT_C
Cuts at: 573 1737 573
Size: 1164 3569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4733
FspI TGC'GCA
Cuts at: 2514 2514
Size: 4733
HaeII r_GCGC'y
Cuts at: 556 1298 2167 2415 3645 4416 556
Size: 742 869 248 1230 771 873
Fragments arranged by size:
1230 873 869 771 742 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 1315 1347 1474 1506 2575 2605 2607
Size: 32 862 32 127 32 1069 30 2
Cuts at: 2607 2637 421
Size: 30 2517
Fragments arranged by size:
2517 1069 862 127 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1772 1772
Size: 4733
KasI G'GCGC_C
Cuts at: 552 1294 2163 2411 552
Size: 742 869 248 2874
Fragments arranged by size:
2874 869 742 248
KpnI G_GTAC'C
Cuts at: 1753 1753
Size: 4733
MluI A'CGCG_T
Cuts at: 4166 4166
Size: 4733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1306 1334 3492 3676 1306
Size: 28 2158 184 2363
Fragments arranged by size:
2363 2158 184 28
MscI TGG'CCA
Cuts at: 2494 2494
Size: 4733
MslI CAynn'nnrTG
Cuts at: 1012 1219 2722 2849 1012
Size: 207 1503 127 2896
Fragments arranged by size:
2896 1503 207 127
MspA1I CmG'CkG
Cuts at: 297 503 686 1844 2158 2518 3302 3547 297
Size: 206 183 1158 314 360 784 245 1483
Fragments arranged by size:
1483 1158 784 360 314 245 206 183
NaeI GCC'GGC
Cuts at: 1293 2914 1293
Size: 1621 3112
NarI GG'CG_CC
Cuts at: 553 1295 2164 2412 553
Size: 742 869 248 2874
Fragments arranged by size:
2874 869 742 248
NcoI C'CATG_G
Cuts at: 1464 1464
Size: 4733
NgoMIV G'CCGG_C
Cuts at: 1291 2912 1291
Size: 1621 3112
NspI r_CATG'y
Cuts at: 1786 2286 3889 1786
Size: 500 1603 2630
Fragments arranged by size:
2630 1603 500
PasI CC'CwG_GG
Cuts at: 812 1555 812
Size: 743 3990
PciI A'CATG_T
Cuts at: 3885 3885
Size: 4733
PflMI CCAn_nnn'nTGG
Cuts at: 631 760 795 1542 631
Size: 129 35 747 3822
Fragments arranged by size:
3822 747 129 35
PfoI T'CCnGG_A
Cuts at: 539 1541 1610 539
Size: 1002 69 3662
Fragments arranged by size:
3662 1002 69
PmeI GTTT'AAAC
Cuts at: 1732 1732
Size: 4733
PmlI CAC'GTG
Cuts at: 1515 1515
Size: 4733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4701
PspOMI G'GGCC_C
Cuts at: 460 1456 460
Size: 996 3737
PstI C_TGCA'G
Cuts at: 508 593 900 909 1780 2465 508
Size: 85 307 9 871 685 2776
Fragments arranged by size:
2776 871 685 307 85 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4733
PvuII CAG'CTG
Cuts at: 503 2158 2518 503
Size: 1655 360 2718
Fragments arranged by size:
2718 1655 360
RsrII CG'GwC_CG
Cuts at: 408 2928 408
Size: 2520 2213
SacI G_AGCT'C
Cuts at: 1747 1747
Size: 4733
SalI G'TCGA_C
Cuts at: 1770 1770
Size: 4733
SapI GCTCTTCn'nnn_
Cuts at: 2756 2966 2756
Size: 210 4523
SbfI CC_TGCA'GG
Cuts at: 1780 1780
Size: 4733
ScaI AGT'ACT
Cuts at: 319 4250 319
Size: 3931 802
SfcI C'TryA_G
Cuts at: 33 504 589 896 905 1776 2461 3429 3620
Size: 471 85 307 9 871 685 968 191
Cuts at: 3620 33
Size: 1146
Fragments arranged by size:
1146 968 871 685 471 307 191 85
9
SfoI GGC'GCC
Cuts at: 554 1296 2165 2413 554
Size: 742 869 248 2874
Fragments arranged by size:
2874 869 742 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
SgrAI Cr'CCGG_yG
Cuts at: 1291 1291
Size: 4733
SmaI CCC'GGG
Cuts at: 1755 1755
Size: 4733
SmlI C'TyrA_G
Cuts at: 1888 2803 3517 3779 1888
Size: 915 714 262 2842
Fragments arranged by size:
2842 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4733
SphI G_CATG'C
Cuts at: 1786 2286 1786
Size: 500 4233
StyI C'CwwG_G
Cuts at: 616 1217 1347 1445 1464 1867 2221 616
Size: 601 130 98 19 403 354 3128
Fragments arranged by size:
3128 601 403 354 130 98 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 300 2316 2449 2908 3117 300
Size: 2016 133 459 209 1916
Fragments arranged by size:
2016 1916 459 209 133
TatI w'GTAC_w
Cuts at: 317 4248 317
Size: 3931 802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4250 4282 4250
Size: 32 4701
Tth111I GACn'n_nGTC
Cuts at: 2530 2530
Size: 4733
XbaI T'CTAG_A
Cuts at: 1764 1764
Size: 4733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1443 2228 1443
Size: 785 3948
XmaI C'CCGG_G
Cuts at: 1753 1753
Size: 4733
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI