Restriction map for
pF1KA1256
(Circular) (Six-base) MAPSORT of: pF1KA1256.seq Check: 8755 from: 1 to: 8123
pF1KA1256 8123 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3405 3405
Size: 8123
AccI GT'mk_AC
Cuts at: 720 4589 5161 720
Size: 3869 572 3682
Fragments arranged by size:
3869 3682 572
Acc65I G'GTAC_C
Cuts at: 1770 5139 1770
Size: 3369 4754
AclI AA'CG_TT
Cuts at: 7963 7963
Size: 8123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 197 391 524 910 1618 1723 3194 3694
Size: 9 194 133 386 708 105 1471 500
Cuts at: 3694 3785 3850 4706 5948 6380 6733 7395 188
Size: 91 65 856 1242 432 353 662 916
Fragments arranged by size:
1471 1242 916 856 708 662 500 432
386 353 194 133 105 91 65 9
AflIII A'CryG_T
Cuts at: 149 5475 7275 7556 149
Size: 5326 1800 281 716
Fragments arranged by size:
5326 1800 716 281
AlwNI CAG_nnn'CTG
Cuts at: 738 2596 3938 5428 6866 738
Size: 1858 1342 1490 1438 1995
Fragments arranged by size:
1995 1858 1490 1438 1342
ApaI G_GGCC'C
Cuts at: 4322 4322
Size: 8123
ApaLI G'TGCA_C
Cuts at: 6961 6961
Size: 8123
ApoI r'AATT_y
Cuts at: 859 1499 2207 2436 3107 4006 4875 4982 4997
Size: 640 708 229 671 899 869 107 15
Cuts at: 4997 5127 7476 7732 859
Size: 130 2349 256 1250
Fragments arranged by size:
2349 1250 899 869 708 671 640 256
229 130 107 15
AseI AT'TA_AT
Cuts at: 20 442 1127 3268 3488 4613 20
Size: 422 685 2141 220 1125 3530
Fragments arranged by size:
3530 2141 1125 685 422 220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8123
AvaI C'yCGr_G
Cuts at: 4171 5143 4171
Size: 972 7151
BamHI G'GATC_C
Cuts at: 4247 5148 4247
Size: 901 7222
BanI G'GyrC_C
Cuts at: 1770 4733 5139 5553 5801 5836 1770
Size: 2963 406 414 248 35 4057
Fragments arranged by size:
4057 2963 414 406 248 35
BanII G_rGCy'C
Cuts at: 3464 3806 4322 4825 5137 6167 3464
Size: 342 516 503 312 1030 5420
Fragments arranged by size:
5420 1030 516 503 342 312
BbeI G_GCGC'C
Cuts at: 4737 5557 5805 4737
Size: 820 248 7055
Fragments arranged by size:
7055 820 248
BbsI GAAGACnn'nnnn_
Cuts at: 521 3859 3985 3995 4554 521
Size: 3338 126 10 559 4090
Fragments arranged by size:
4090 3338 559 126 10
BbvCI CC'TCA_GC
Cuts at: 491 830 2231 2270 491
Size: 339 1401 39 6344
Fragments arranged by size:
6344 1401 339 39
BciVI GTATCCnnnnn_n'
Cuts at: 2250 6021 7077 8123 2250
Size: 3771 1056 1046 2250
Fragments arranged by size:
3771 2250 1056 1046
BclI T'GATC_A
Cuts at: 234 5643 234
Size: 5409 2714
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 8123
BglII A'GATC_T
Cuts at: 1619 5638 7675 1619
Size: 4019 2037 2067
Fragments arranged by size:
4019 2067 2037
BlpI GC'TnA_GC
Cuts at: 3905 5235 3905
Size: 1330 6793
Bme1580I G_kGCm'C
Cuts at: 4322 5748 5841 6965 4322
Size: 1426 93 1124 5480
Fragments arranged by size:
5480 1426 1124 93
BmgBI CAC'GTC
Cuts at: 4917 4917
Size: 8123
BmrI ACTGGGnnnn_n'
Cuts at: 815 5335 5416 5751 7895 815
Size: 4520 81 335 2144 1043
Fragments arranged by size:
4520 2144 1043 335 81
BmtI G_CTAG'C
Cuts at: 3164 3164
Size: 8123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4209 6490 4209
Size: 2281 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 2167 2387 3038 3191 4041 4141 4261 488
Size: 1679 220 651 153 850 100 120 4350
Fragments arranged by size:
4350 1679 850 651 220 153 120 100
Bpu10I CC'TnA_GC
Cuts at: 491 830 1079 2231 2270 3200 491
Size: 339 249 1152 39 930 5414
Fragments arranged by size:
5414 1152 930 339 249 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 4181 5299 6178 6892 7190 1606
Size: 2575 1118 879 714 298 2539
Fragments arranged by size:
2575 2539 1118 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3553 4937 7827 3553
Size: 1384 2890 3849
Fragments arranged by size:
3849 2890 1384
BsaAI yAC'GTr
Cuts at: 244 4260 5476 244
Size: 4016 1216 2891
Fragments arranged by size:
4016 2891 1216
BsaBI GATnn'nnATC
Cuts at: 6 2688 5637 7674 6
Size: 2682 2949 2037 455
Fragments arranged by size:
2949 2682 2037 455
BsaHI Gr'CG_yC
Cuts at: 1251 4651 4734 5554 5802 6504 8000 1251
Size: 3400 83 820 248 702 1496 1374
Fragments arranged by size:
3400 1496 1374 820 702 248 83
BsaWI w'CCGG_w
Cuts at: 5312 5833 6511 6922 7069 7343 5312
Size: 521 678 411 147 274 6092
Fragments arranged by size:
6092 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1059 1089 4138 4168 1059
Size: 30 3049 30 5014
Fragments arranged by size:
5014 3049 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1118 1180 2248 2287 4198 1118
Size: 62 1068 39 1911 5043
Fragments arranged by size:
5043 1911 1068 62 39
BseYI C'CCAG_C
Cuts at: 5548 6306 6971 7379 5548
Size: 758 665 408 6292
Fragments arranged by size:
6292 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 3898 4282 4360 4654 494
Size: 3404 384 78 294 3963
Fragments arranged by size:
3963 3404 384 294 78
BsiEI CG_ry'CG
Cuts at: 104 5665 5711 6941 104
Size: 5561 46 1230 1286
Fragments arranged by size:
5561 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3464 3806 4195 5137 5915 6105 6965 3464
Size: 342 389 942 778 190 860 4622
Fragments arranged by size:
4622 942 860 778 389 342 190
BsmI GAATG_Cn'
Cuts at: 4052 4052
Size: 8123
Bsp1286I G_dGCh'C
Cuts at: 3464 3806 4195 4322 4825 5137 5748 5841 5915
Size: 342 389 127 503 312 611 93 74
Cuts at: 5915 6105 6167 6965 3464
Size: 190 62 798 4622
Fragments arranged by size:
4622 798 611 503 389 342 312 190
127 93 74 62
BspHI T'CATG_A
Cuts at: 3669 6555 3669
Size: 2886 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3405 5096 5173 5387 5689 6070 3405
Size: 1691 77 214 302 381 5458
Fragments arranged by size:
5458 1691 381 302 214 77
BsrBI CCG'CTC
Cuts at: 50 3693 4176 4709 6415 6469 7953 8118 50
Size: 3643 483 533 1706 54 1484 165 55
Fragments arranged by size:
3643 1706 1484 533 483 165 55 54
BsrDI GCAATG_nn'
Cuts at: 496 664 1028 6035 496
Size: 168 364 5007 2584
Fragments arranged by size:
5007 2584 364 168
BsrFI r'CCGG_y
Cuts at: 270 3873 6121 6302 270
Size: 3603 2248 181 2091
Fragments arranged by size:
3603 2248 2091 181
BssSI C'ACGA_G
Cuts at: 6394 7102 6394
Size: 708 7415
BstAPI GCAn_nnn'nTGC
Cuts at: 3220 5500 3220
Size: 2280 5843
BstBI TT'CG_AA
Cuts at: 5001 6484 5001
Size: 1483 6640
BstEII G'GTnAC_C
Cuts at: 3054 3054
Size: 8123
BstXI CCAn_nnnn'nTGG
Cuts at: 152 3067 3955 4314 152
Size: 2915 888 359 3961
Fragments arranged by size:
3961 2915 888 359
BstYI r'GATC_y
Cuts at: 1 751 1619 2683 3588 4247 4902 5148 5615
Size: 750 868 1064 905 659 655 246 467
Cuts at: 5615 5638 5973 6219 6525 6537 6623 7675 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1064 1052 905 868 750 659 655 467
449 335 306 246 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 721 721
Size: 8123
BtgI C'CryG_G
Cuts at: 4033 4033
Size: 8123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4565 6073 6254 6417 4565
Size: 1508 181 163 6271
Fragments arranged by size:
6271 1508 181 163
BtsI GCAGTG_nn'
Cuts at: 1699 4099 4270 5366 1699
Size: 2400 171 1096 4456
Fragments arranged by size:
4456 2400 1096 171
DraI TTT'AAA
Cuts at: 4013 5122 4013
Size: 1109 7014
DrdI GACnn_nn'nnGTC
Cuts at: 5829 7173 5829
Size: 1344 6779
EaeI y'GGCC_r
Cuts at: 1040 3899 5708 5882 6273 6300 7645 1040
Size: 2859 1809 174 391 27 1345 1518
Fragments arranged by size:
2859 1809 1518 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5708 5708
Size: 8123
EarI CTCTTCn'nnn_
Cuts at: 1001 1157 1307 2210 2847 3683 4393 6146 6356
Size: 156 150 903 637 836 710 1753 210
Cuts at: 6356 1001
Size: 2768
Fragments arranged by size:
2768 1753 903 836 710 637 210 156
150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6331 7073 7219 7933 6331
Size: 742 146 714 6521
Fragments arranged by size:
6521 742 714 146
EcoICRI GAG'CTC
Cuts at: 3462 3804 5135 3462
Size: 342 1331 6450
Fragments arranged by size:
6450 1331 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 197 391 488 524 910 1618 1723 2167
Size: 9 194 97 36 386 708 105 444
Cuts at: 2167 2387 3038 3191 3194 3694 3785 3850 4041
Size: 220 651 153 3 500 91 65 191
Cuts at: 4041 4141 4261 4706 5948 6380 6733 7395 188
Size: 100 120 445 1242 432 353 662 916
Fragments arranged by size:
1242 916 708 662 651 500 445 444
432 386 353 220 194 191 153 120
105 100 97 91 65 36 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 215 313 1832 215
Size: 98 1519 6506
Fragments arranged by size:
6506 1519 98
EcoO109I rG'GnC_Cy
Cuts at: 809 2739 4318 4319 5023 5055 5262 809
Size: 1930 1579 1 704 32 207 3670
Fragments arranged by size:
3670 1930 1579 704 207 32 1
EcoRI G'AATT_C
Cuts at: 1499 5127 1499
Size: 3628 4495
EcoRV GAT'ATC
Cuts at: 892 892
Size: 8123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2532 6142 2164
Size: 368 3610 4145
Fragments arranged by size:
4145 3610 368
FspI TGC'GCA
Cuts at: 5904 5904
Size: 8123
HaeII r_GCGC'y
Cuts at: 4737 5557 5805 7035 7806 4737
Size: 820 248 1230 771 5054
Fragments arranged by size:
5054 1230 820 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 558 590 736 768 876 908 1059 1091 2586
Size: 32 146 32 108 32 151 32 1495
Cuts at: 2586 2618 4138 4170 5965 5995 5997 6027 558
Size: 32 1520 32 1795 30 2 30 2654
Fragments arranged by size:
2654 1795 1520 1495 151 146 108 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3598 3792 5162 3598
Size: 194 1370 6559
Fragments arranged by size:
6559 1370 194
HindIII A'AGCT_T
Cuts at: 373 1756 2921 4694 373
Size: 1383 1165 1773 3802
Fragments arranged by size:
3802 1773 1383 1165
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 8123
KasI G'GCGC_C
Cuts at: 4733 5553 5801 4733
Size: 820 248 7055
Fragments arranged by size:
7055 820 248
KpnI G_GTAC'C
Cuts at: 1774 5143 1774
Size: 3369 4754
MfeI C'AATT_G
Cuts at: 3641 3641
Size: 8123
MluI A'CGCG_T
Cuts at: 7556 7556
Size: 8123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1571 1668 2952 3839 6882 7066 1571
Size: 97 1284 887 3043 184 2628
Fragments arranged by size:
3043 2628 1284 887 184 97
MscI TGG'CCA
Cuts at: 1042 5884 1042
Size: 4842 3281
MslI CAynn'nnrTG
Cuts at: 130 352 6112 6239 130
Size: 222 5760 127 2014
Fragments arranged by size:
5760 2014 222 127
MspA1I CmG'CkG
Cuts at: 508 951 2935 3904 3944 4035 5234 5548 5908
Size: 443 1984 969 40 91 1199 314 360
Cuts at: 5908 6692 6937 508
Size: 784 245 1694
Fragments arranged by size:
1984 1694 1199 969 784 443 360 314
245 91 40
NaeI GCC'GGC
Cuts at: 272 6304 272
Size: 6032 2091
NarI GG'CG_CC
Cuts at: 4734 5554 5802 4734
Size: 820 248 7055
Fragments arranged by size:
7055 820 248
NdeI CA'TA_TG
Cuts at: 3216 3216
Size: 8123
NgoMIV G'CCGG_C
Cuts at: 270 6302 270
Size: 6032 2091
NheI G'CTAG_C
Cuts at: 3160 3160
Size: 8123
NspI r_CATG'y
Cuts at: 153 474 1812 3921 4750 5176 5676 7279 153
Size: 321 1338 2109 829 426 500 1603 997
Fragments arranged by size:
2109 1603 1338 997 829 500 426 321
PciI A'CATG_T
Cuts at: 149 7275 149
Size: 7126 997
PfoI T'CCnGG_A
Cuts at: 2183 2749 4850 2183
Size: 566 2101 5456
Fragments arranged by size:
5456 2101 566
PmeI GTTT'AAAC
Cuts at: 5122 5122
Size: 8123
PmlI CAC'GTG
Cuts at: 4260 4260
Size: 8123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6569 6601 6569
Size: 32 8091
PpuMI rG'GwC_Cy
Cuts at: 809 5055 809
Size: 4246 3877
PsiI TTA'TAA
Cuts at: 2021 2021
Size: 8123
PspOMI G'GGCC_C
Cuts at: 4318 4318
Size: 8123
PspXI vC'TCGA_Gb
Cuts at: 4171 4171
Size: 8123
PstI C_TGCA'G
Cuts at: 3943 5093 5170 5855 3943
Size: 1150 77 685 6211
Fragments arranged by size:
6211 1150 685 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8123
PvuII CAG'CTG
Cuts at: 508 951 2935 3944 5548 5908 508
Size: 443 1984 1009 1604 360 2723
Fragments arranged by size:
2723 1984 1604 1009 443 360
RsrII CG'GwC_CG
Cuts at: 4233 6318 4233
Size: 2085 6038
SacI G_AGCT'C
Cuts at: 3464 3806 5137 3464
Size: 342 1331 6450
Fragments arranged by size:
6450 1331 342
SacII CC_GC'GG
Cuts at: 4036 4036
Size: 8123
SalI G'TCGA_C
Cuts at: 5160 5160
Size: 8123
SapI GCTCTTCn'nnn_
Cuts at: 3683 6146 6356 3683
Size: 2463 210 5450
Fragments arranged by size:
5450 2463 210
SbfI CC_TGCA'GG
Cuts at: 5170 5170
Size: 8123
ScaI AGT'ACT
Cuts at: 7640 7640
Size: 8123
SexAI A'CCwGG_T
Cuts at: 2406 2406
Size: 8123
SfcI C'TryA_G
Cuts at: 33 2528 3939 4760 5089 5166 5851 6819 7010
Size: 2495 1411 821 329 77 685 968 191
Cuts at: 7010 33
Size: 1146
Fragments arranged by size:
2495 1411 1146 968 821 685 329 191
77
SfoI GGC'GCC
Cuts at: 4735 5555 5803 4735
Size: 820 248 7055
Fragments arranged by size:
7055 820 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8123
SmaI CCC'GGG
Cuts at: 5145 5145
Size: 8123
SmlI C'TyrA_G
Cuts at: 1585 4171 4196 5278 6193 6907 7169 1585
Size: 2586 25 1082 915 714 262 2539
Fragments arranged by size:
2586 2539 1082 915 714 262 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8123
SphI G_CATG'C
Cuts at: 474 5176 5676 474
Size: 4702 500 2921
Fragments arranged by size:
4702 2921 500
SspI AAT'ATT
Cuts at: 3050 3266 3050
Size: 216 7907
StuI AGG'CCT
Cuts at: 2515 4274 2515
Size: 1759 6364
StyI C'CwwG_G
Cuts at: 3473 5257 5611 3473
Size: 1784 354 5985
Fragments arranged by size:
5985 1784 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 813 3281 3700 4250 4620 5706 5839 6298 6507
Size: 2468 419 550 370 1086 133 459 209
Cuts at: 6507 813
Size: 2429
Fragments arranged by size:
2468 2429 1086 550 459 419 370 209
133
TatI w'GTAC_w
Cuts at: 4661 7638 4661
Size: 2977 5146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1678 1710 4848 4880 7640 7672 1678
Size: 32 3138 32 2760 32 2129
Fragments arranged by size:
3138 2760 2129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1478 5920 1478
Size: 4442 3681
XbaI T'CTAG_A
Cuts at: 5154 5154
Size: 8123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3599 5618 3599
Size: 2019 6104
XhoI C'TCGA_G
Cuts at: 4171 4171
Size: 8123
XmaI C'CCGG_G
Cuts at: 5143 5143
Size: 8123
XmnI GAAnn'nnTTC
Cuts at: 2207 4456 2207
Size: 2249 5874
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BsiWI BsmBI BspEI
BsrGI BssHII Bsu36I ClaI CspCI DraIII FseI FspAI
NcoI NotI NruI NsiI PacI PasI PflMI PshAI
PsrI SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI