Restriction map for
pF1KA1238
(Circular) (Six-base) MAPSORT of: pF1KA1238.seq Check: 137 from: 1 to: 4271
pF1KA1238 4271 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 2096 2528 3275 3543 572
Size: 1524 432 747 268 1300
Fragments arranged by size:
1524 1300 747 432 268
AflIII A'CryG_T
Cuts at: 720 1623 2727 3704 720
Size: 903 1104 977 1287
Fragments arranged by size:
1287 1104 977 903
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AhdI GACnn_n'nnGTC
Cuts at: 149 149
Size: 4271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 916 916
Size: 4271
AlwNI CAG_nnn'CTG
Cuts at: 1576 3143 1576
Size: 1567 2704
ApaLI G'TGCA_C
Cuts at: 3041 3041
Size: 4271
ApoI r'AATT_y
Cuts at: 525 1275 3624 3880 525
Size: 750 2349 256 916
Fragments arranged by size:
2349 916 750 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 620 1291 620
Size: 671 3600
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 751 1287 1701 1949 1984 751
Size: 536 414 248 35 3038
Fragments arranged by size:
3038 536 414 248 35
BanII G_rGCy'C
Cuts at: 1285 2315 1285
Size: 1030 3241
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BbsI GAAGACnn'nnnn_
Cuts at: 170 170
Size: 4271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 511 545 511
Size: 34 4237
BciVI GTATCCnnnnn_n'
Cuts at: 492 2169 2930 4271 492
Size: 1677 761 1341 492
Fragments arranged by size:
1677 1341 761 492
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 1896 1989 3045 1896
Size: 93 1056 3122
Fragments arranged by size:
3122 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1483 1564 1899 4043 1483
Size: 81 335 2144 1711
Fragments arranged by size:
2144 1711 335 81
BmtI G_CTAG'C
Cuts at: 853 1240 853
Size: 387 3884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1210 2638 1210
Size: 1428 2843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 580
Size: 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 1447 2326 2818 3116 410
Size: 1037 879 492 298 1565
Fragments arranged by size:
1565 1037 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3975 3975
Size: 4271
BsaAI yAC'GTr
Cuts at: 721 1624 721
Size: 903 3368
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 1702 1950 2652 4148 1702
Size: 248 702 1496 1825
Fragments arranged by size:
1825 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 1981 2659 2933 3080 3491 1460
Size: 521 678 274 147 411 2240
Fragments arranged by size:
2240 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 728 758 728
Size: 30 4241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 4271
BseYI C'CCAG_C
Cuts at: 1104 1113 1696 2454 3031 3527 1104
Size: 9 583 758 577 496 1848
Fragments arranged by size:
1848 758 583 577 496 9
BsiEI CG_ry'CG
Cuts at: 104 1813 1859 3067 104
Size: 1709 46 1208 1308
Fragments arranged by size:
1709 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 826 1285 2063 2253 3045 826
Size: 459 778 190 792 2052
Fragments arranged by size:
2052 792 778 459 190
BsmI GAATG_Cn'
Cuts at: 788 788
Size: 4271
Bsp1286I G_dGCh'C
Cuts at: 826 1285 1896 1989 2063 2253 2315 3045 826
Size: 459 611 93 74 190 62 730 2052
Fragments arranged by size:
2052 730 611 459 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 908 1321 1535 1837 2218 908
Size: 413 214 302 381 2961
Fragments arranged by size:
2961 413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2183 2183
Size: 4271
BsrFI r'CCGG_y
Cuts at: 1037 2269 2450 2659 1037
Size: 1232 181 209 2649
Fragments arranged by size:
2649 1232 209 181
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 1012 1648 1012
Size: 636 3635
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 350 350
Size: 4271
BstYI r'GATC_y
Cuts at: 1 1296 1763 1786 2121 2367 3379 3465 3477
Size: 1295 467 23 335 246 1012 86 12
Cuts at: 3477 3823 1
Size: 346 449
Fragments arranged by size:
1295 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 480 1067 480
Size: 587 3684
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 945 2221 2402 2565 945
Size: 1276 181 163 2651
Fragments arranged by size:
2651 1276 181 163
BtsI GCAGTG_nn'
Cuts at: 308 1076 1121 1514 308
Size: 768 45 393 3065
Fragments arranged by size:
3065 768 393 45
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 1977 2835 1977
Size: 858 3413
EaeI y'GGCC_r
Cuts at: 477 516 1035 1856 2030 2421 2448 3793 477
Size: 39 519 821 174 391 27 1345 955
Fragments arranged by size:
1345 955 821 519 391 174 39 27
EagI C'GGCC_G
Cuts at: 1856 1856
Size: 4271
EarI CTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2479 2789 2935 4081 2479
Size: 310 146 1146 2669
Fragments arranged by size:
2669 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 580 2096 2528 3275 3543 572
Size: 8 1516 432 747 268 1300
Fragments arranged by size:
1516 1300 747 432 268 8
EcoO109I rG'GnC_Cy
Cuts at: 1108 1212 1410 1108
Size: 104 198 3969
Fragments arranged by size:
3969 198 104
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 673 1705 1953 2975 3954 673
Size: 1032 248 1022 979 990
Fragments arranged by size:
1032 1022 990 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 290 322 390 422 490 522 904
Size: 32 123 32 68 32 68 32 382
Cuts at: 904 936 2113 2143 2145 2175 135
Size: 32 1177 30 2 30 2231
Fragments arranged by size:
2231 1177 382 123 68 68 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1131 1161 1310 1131
Size: 30 149 4092
Fragments arranged by size:
4092 149 30
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2942 3126 2942
Size: 184 4087
MscI TGG'CCA
Cuts at: 479 518 2032 479
Size: 39 1514 2718
Fragments arranged by size:
2718 1514 39
MslI CAynn'nnrTG
Cuts at: 730 2260 2387 730
Size: 1530 127 2614
Fragments arranged by size:
2614 1530 127
MspA1I CmG'CkG
Cuts at: 1233 1382 1696 2056 3069 3314 1233
Size: 149 314 360 1013 245 2190
Fragments arranged by size:
2190 1013 360 314 245 149
NaeI GCC'GGC
Cuts at: 1039 2452 1039
Size: 1413 2858
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 480 480
Size: 4271
NgoMIV G'CCGG_C
Cuts at: 1037 2450 1037
Size: 1413 2858
NheI G'CTAG_C
Cuts at: 849 1236 849
Size: 387 3884
NspI r_CATG'y
Cuts at: 1324 1824 2731 1324
Size: 500 907 2864
Fragments arranged by size:
2864 907 500
PasI CC'CwG_GG
Cuts at: 1207 1207
Size: 4271
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PflMI CCAn_nnn'nTGG
Cuts at: 134 134
Size: 4271
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1131 1163 3410 3442 1131
Size: 32 2247 32 1960
Fragments arranged by size:
2247 1960 32 32
PpuMI rG'GwC_Cy
Cuts at: 1108 1108
Size: 4271
PstI C_TGCA'G
Cuts at: 1318 2003 1318
Size: 685 3586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 1233 1696 2056 1233
Size: 463 360 3448
Fragments arranged by size:
3448 463 360
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SanDI GG'GwC_CC
Cuts at: 1108 1108
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SfcI C'TryA_G
Cuts at: 33 1314 1999 2992 3183 33
Size: 1281 685 993 191 1121
Fragments arranged by size:
1281 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 425 620 1426 2341 2833 3095 425
Size: 195 806 915 492 262 1601
Fragments arranged by size:
1601 915 806 492 262 195
SpeI A'CTAG_T
Cuts at: 67 1090 67
Size: 1023 3248
SphI G_CATG'C
Cuts at: 1324 1824 1324
Size: 500 3771
StyI C'CwwG_G
Cuts at: 349 480 1405 1759 349
Size: 131 925 354 2861
Fragments arranged by size:
2861 925 354 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 1854 1987 2446 2655 342
Size: 1512 133 459 209 1958
Fragments arranged by size:
1958 1512 459 209 133
TatI w'GTAC_w
Cuts at: 3786 3786
Size: 4271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 728 760 3788 3820 728
Size: 32 3028 32 1179
Fragments arranged by size:
3028 1179 32 32
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 390 984 1302 390
Size: 594 318 3359
Fragments arranged by size:
3359 594 318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1766 1766
Size: 4271
XhoI C'TCGA_G
Cuts at: 620 620
Size: 4271
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI Bpu10I BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI