Restriction map for
pF1KA1221
(Circular) (Six-base) MAPSORT of: pF1KA1221.seq Check: 1490 from: 1 to: 7094
pF1KA1221 7094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1311 1311
Size: 7094
AccI GT'mk_AC
Cuts at: 598 1076 1112 4132 598
Size: 478 36 3020 3560
Fragments arranged by size:
3560 3020 478 36
Acc65I G'GTAC_C
Cuts at: 1618 4110 1618
Size: 2492 4602
AclI AA'CG_TT
Cuts at: 6934 6934
Size: 7094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 574 686 1552 1877 3530 4070 4919 5351
Size: 221 112 866 325 1653 540 849 432
Cuts at: 5351 6098 6366 353
Size: 747 268 1081
Fragments arranged by size:
1653 1081 866 849 747 540 432 325
268 221 112
AflII C'TTAA_G
Cuts at: 366 366
Size: 7094
AflIII A'CryG_T
Cuts at: 2198 2520 4446 5550 6527 2198
Size: 322 1926 1104 977 2765
Fragments arranged by size:
2765 1926 1104 977 322
AgeI A'CCGG_T
Cuts at: 5482 5482
Size: 7094
AleI CACnn'nnGTG
Cuts at: 2519 2519
Size: 7094
AlwNI CAG_nnn'CTG
Cuts at: 3526 4399 5966 3526
Size: 873 1567 4654
Fragments arranged by size:
4654 1567 873
ApaLI G'TGCA_C
Cuts at: 1586 5864 1586
Size: 4278 2816
ApoI r'AATT_y
Cuts at: 723 836 1359 1547 2135 2415 2769 3549 3660
Size: 113 523 188 588 280 354 780 111
Cuts at: 3660 4098 6447 6703 723
Size: 438 2349 256 1114
Fragments arranged by size:
2349 1114 780 588 523 438 354 280
256 188 113 111
AseI AT'TA_AT
Cuts at: 20 2753 20
Size: 2733 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7094
AvaI C'yCGr_G
Cuts at: 4114 4114
Size: 7094
BamHI G'GATC_C
Cuts at: 885 4119 885
Size: 3234 3860
BanI G'GyrC_C
Cuts at: 1618 4110 4524 4772 4807 1618
Size: 2492 414 248 35 3905
Fragments arranged by size:
3905 2492 414 248 35
BanII G_rGCy'C
Cuts at: 4108 5138 4108
Size: 1030 6064
BbeI G_GCGC'C
Cuts at: 4528 4776 4528
Size: 248 6846
BbsI GAAGACnn'nnnn_
Cuts at: 1169 1533 2585 3947 1169
Size: 364 1052 1362 4316
Fragments arranged by size:
4316 1362 1052 364
BbvCI CC'TCA_GC
Cuts at: 2915 2915
Size: 7094
BciVI GTATCCnnnnn_n'
Cuts at: 4992 5753 7094 4992
Size: 761 1341 4992
Fragments arranged by size:
4992 1341 761
BclI T'GATC_A
Cuts at: 2354 4614 2354
Size: 2260 4834
BfrBI ATG'CAT
Cuts at: 1129 1129
Size: 7094
BglI GCCn_nnn'nGGC
Cuts at: 1739 1739
Size: 7094
BglII A'GATC_T
Cuts at: 3829 4609 6646 3829
Size: 780 2037 4277
Fragments arranged by size:
4277 2037 780
BlpI GC'TnA_GC
Cuts at: 4206 4206
Size: 7094
Bme1580I G_kGCm'C
Cuts at: 1590 4719 4812 5868 1590
Size: 3129 93 1056 2816
Fragments arranged by size:
3129 2816 1056 93
BmgBI CAC'GTC
Cuts at: 2521 4019 2521
Size: 1498 5596
BmrI ACTGGGnnnn_n'
Cuts at: 891 3584 4306 4387 4722 6866 891
Size: 2693 722 81 335 2144 1119
Fragments arranged by size:
2693 2144 1119 722 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5461 5461
Size: 7094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 4016 2834
Size: 1182 5912
Bpu10I CC'TnA_GC
Cuts at: 2915 2915
Size: 7094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3007 4270 5149 5641 5939 3007
Size: 1263 879 492 298 4162
Fragments arranged by size:
4162 1263 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6798 6798
Size: 7094
BsaAI yAC'GTr
Cuts at: 4447 4447
Size: 7094
BsaBI GATnn'nnATC
Cuts at: 6 2591 2753 4608 6645 6
Size: 2585 162 1855 2037 455
Fragments arranged by size:
2585 2037 1855 455 162
BsaHI Gr'CG_yC
Cuts at: 2486 4525 4773 5475 6971 2486
Size: 2039 248 702 1496 2609
Fragments arranged by size:
2609 2039 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4283 4804 5482 5756 5903 6314 4283
Size: 521 678 274 147 411 5063
Fragments arranged by size:
5063 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 437
Size: 30 7064
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 465 2210 3665 465
Size: 1745 1455 3894
Fragments arranged by size:
3894 1745 1455
BseYI C'CCAG_C
Cuts at: 3012 4519 5277 5854 6350 3012
Size: 1507 758 577 496 3756
Fragments arranged by size:
3756 1507 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 4636 4682 5890 104
Size: 4532 46 1208 1308
Fragments arranged by size:
4532 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 408 1590 4108 4886 5076 5868 408
Size: 1182 2518 778 190 792 1634
Fragments arranged by size:
2518 1634 1182 792 778 190
BsmI GAATG_Cn'
Cuts at: 1735 1932 1735
Size: 197 6897
Bsp1286I G_dGCh'C
Cuts at: 408 1590 4108 4719 4812 4886 5076 5138 5868
Size: 1182 2518 611 93 74 190 62 730
Cuts at: 5868 408
Size: 1634
Fragments arranged by size:
2518 1634 1182 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 6314 6314
Size: 7094
BspHI T'CATG_A
Cuts at: 1820 6270 1820
Size: 4450 2644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1311 3887 4144 4358 4660 5041 1311
Size: 2576 257 214 302 381 3364
Fragments arranged by size:
3364 2576 381 302 257 214
BsrBI CCG'CTC
Cuts at: 50 269 5386 5440 6924 7089 50
Size: 219 5117 54 1484 165 55
Fragments arranged by size:
5117 1484 219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1359 1742 2177 5006 1359
Size: 383 435 2829 3447
Fragments arranged by size:
3447 2829 435 383
BsrFI r'CCGG_y
Cuts at: 5092 5273 5482 5092
Size: 181 209 6704
Fragments arranged by size:
6704 209 181
BsrGI T'GTAC_A
Cuts at: 1342 1342
Size: 7094
BssSI C'ACGA_G
Cuts at: 5365 5723 5365
Size: 358 6736
BstAPI GCAn_nnn'nTGC
Cuts at: 4471 4471
Size: 7094
BstBI TT'CG_AA
Cuts at: 5455 5455
Size: 7094
BstXI CCAn_nnnn'nTGG
Cuts at: 183 2848 183
Size: 2665 4429
BstYI r'GATC_y
Cuts at: 1 885 3683 3829 4119 4586 4609 4944 5190
Size: 884 2798 146 290 467 23 335 246
Cuts at: 5190 6202 6288 6300 6646 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2798 1012 884 467 449 346 335 290
246 146 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 843 843
Size: 7094
BtgI C'CryG_G
Cuts at: 3988 3988
Size: 7094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5044 5225 5388 5044
Size: 181 163 6750
Fragments arranged by size:
6750 181 163
BtsI GCAGTG_nn'
Cuts at: 516 1715 1763 4337 516
Size: 1199 48 2574 3273
Fragments arranged by size:
3273 2574 1199 48
DraI TTT'AAA
Cuts at: 1910 2078 4093 1910
Size: 168 2015 4911
Fragments arranged by size:
4911 2015 168
DrdI GACnn_nn'nnGTC
Cuts at: 2979 4800 5658 2979
Size: 1821 858 4415
Fragments arranged by size:
4415 1821 858
EaeI y'GGCC_r
Cuts at: 2146 4073 4679 4853 5244 5271 6616 2146
Size: 1927 606 174 391 27 1345 2624
Fragments arranged by size:
2624 1927 1345 606 391 174 27
EagI C'GGCC_G
Cuts at: 4679 4679
Size: 7094
EarI CTCTTCn'nnn_
Cuts at: 1091 1217 1277 1316 1685 5117 5327 1091
Size: 126 60 39 369 3432 210 2858
Fragments arranged by size:
3432 2858 369 210 126 60 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5302 5612 5758 6904 5302
Size: 310 146 1146 5492
Fragments arranged by size:
5492 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4106 4106
Size: 7094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 574 686 1552 1877 2834 3530 4016 4070
Size: 221 112 866 325 957 696 486 54
Cuts at: 4070 4919 5351 6098 6366 353
Size: 849 432 747 268 1081
Fragments arranged by size:
1081 957 866 849 747 696 486 432
325 268 221 112 54
EcoO109I rG'GnC_Cy
Cuts at: 3922 4233 3922
Size: 311 6783
EcoRI G'AATT_C
Cuts at: 723 3660 4098 723
Size: 2937 438 3719
Fragments arranged by size:
3719 2937 438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5113 5113
Size: 7094
FspI TGC'GCA
Cuts at: 4875 4875
Size: 7094
HaeII r_GCGC'y
Cuts at: 4528 4776 5798 6777 4528
Size: 248 1022 979 4845
Fragments arranged by size:
4845 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1156 1188 1282 1314 2895 2927 2980 3012 3445
Size: 32 94 32 1581 32 53 32 433
Cuts at: 3445 3477 3498 3530 3675 3707 4936 4966 4968
Size: 32 21 32 145 32 1229 30 2
Cuts at: 4968 4998 1156
Size: 30 3252
Fragments arranged by size:
3252 1581 1229 433 145 94 53 32
32 32 32 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 323 619 2051 2253 4133 323
Size: 296 1432 202 1880 3284
Fragments arranged by size:
3284 1880 1432 296 202
HindIII A'AGCT_T
Cuts at: 2502 2502
Size: 7094
KasI G'GCGC_C
Cuts at: 4524 4772 4524
Size: 248 6846
KpnI G_GTAC'C
Cuts at: 1622 4114 1622
Size: 2492 4602
MfeI C'AATT_G
Cuts at: 1623 1623
Size: 7094
MluI A'CGCG_T
Cuts at: 6527 6527
Size: 7094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1212 2550 5765 5949 1212
Size: 1338 3215 184 2357
Fragments arranged by size:
3215 2357 1338 184
MscI TGG'CCA
Cuts at: 4855 4855
Size: 7094
MslI CAynn'nnrTG
Cuts at: 401 1406 1433 1708 1941 2228 2336 2519 5083
Size: 1005 27 275 233 287 108 183 2564
Cuts at: 5083 5210 401
Size: 127 2285
Fragments arranged by size:
2564 2285 1005 287 275 233 183 127
108 27
MspA1I CmG'CkG
Cuts at: 583 594 4205 4519 4879 5892 6137 583
Size: 11 3611 314 360 1013 245 1540
Fragments arranged by size:
3611 1540 1013 360 314 245 11
NaeI GCC'GGC
Cuts at: 5275 5275
Size: 7094
NarI GG'CG_CC
Cuts at: 4525 4773 4525
Size: 248 6846
NdeI CA'TA_TG
Cuts at: 1391 1449 1505 1867 1391
Size: 58 56 362 6618
Fragments arranged by size:
6618 362 58 56
NgoMIV G'CCGG_C
Cuts at: 5273 5273
Size: 7094
NsiI A_TGCA'T
Cuts at: 1131 1131
Size: 7094
NspI r_CATG'y
Cuts at: 400 658 1651 2202 2767 4147 4647 5554 400
Size: 258 993 551 565 1380 500 907 1940
Fragments arranged by size:
1940 1380 993 907 565 551 500 258
PciI A'CATG_T
Cuts at: 2198 5550 2198
Size: 3352 3742
PflMI CCAn_nnn'nTGG
Cuts at: 3994 3994
Size: 7094
PmeI GTTT'AAAC
Cuts at: 4093 4093
Size: 7094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6233 6265 6233
Size: 32 7062
PpuMI rG'GwC_Cy
Cuts at: 3922 3922
Size: 7094
PshAI GACnn'nnGTC
Cuts at: 2620 3927 2620
Size: 1307 5787
PsiI TTA'TAA
Cuts at: 1202 1202
Size: 7094
PstI C_TGCA'G
Cuts at: 2934 4141 4826 2934
Size: 1207 685 5202
Fragments arranged by size:
5202 1207 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7094
PvuII CAG'CTG
Cuts at: 583 594 4519 4879 583
Size: 11 3925 360 2798
Fragments arranged by size:
3925 2798 360 11
RsrII CG'GwC_CG
Cuts at: 5289 5289
Size: 7094
SacI G_AGCT'C
Cuts at: 4108 4108
Size: 7094
SalI G'TCGA_C
Cuts at: 4131 4131
Size: 7094
SapI GCTCTTCn'nnn_
Cuts at: 1091 1316 5117 5327 1091
Size: 225 3801 210 2858
Fragments arranged by size:
3801 2858 225 210
SbfI CC_TGCA'GG
Cuts at: 4141 4141
Size: 7094
ScaI AGT'ACT
Cuts at: 1927 6611 1927
Size: 4684 2410
SfcI C'TryA_G
Cuts at: 33 1832 2009 2346 2742 2930 3191 4137 4822
Size: 1799 177 337 396 188 261 946 685
Cuts at: 4822 5815 6006 33
Size: 993 191 1121
Fragments arranged by size:
1799 1121 993 946 685 396 337 261
191 188 177
SfoI GGC'GCC
Cuts at: 4526 4774 4526
Size: 248 6846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7094
SmaI CCC'GGG
Cuts at: 4116 4116
Size: 7094
SmlI C'TyrA_G
Cuts at: 366 2986 4249 5164 5656 5918 366
Size: 2620 1263 915 492 262 1542
Fragments arranged by size:
2620 1542 1263 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7094
SphI G_CATG'C
Cuts at: 4147 4647 4147
Size: 500 6594
StuI AGG'CCT
Cuts at: 2496 2496
Size: 7094
StyI C'CwwG_G
Cuts at: 1441 2303 4228 4582 1441
Size: 862 1925 354 3953
Fragments arranged by size:
3953 1925 862 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 685 769 4677 4810 5269 5478 685
Size: 84 3908 133 459 209 2301
Fragments arranged by size:
3908 2301 459 209 133 84
TatI w'GTAC_w
Cuts at: 1342 1925 6609 1342
Size: 583 4684 1827
Fragments arranged by size:
4684 1827 583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 521 553 6611 6643 521
Size: 32 6058 32 972
Fragments arranged by size:
6058 972 32 32
Tth111I GACn'n_nGTC
Cuts at: 4891 4891
Size: 7094
XbaI T'CTAG_A
Cuts at: 148 966 1083 4125 148
Size: 818 117 3042 3117
Fragments arranged by size:
3117 3042 818 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4589 4589
Size: 7094
XmaI C'CCGG_G
Cuts at: 4114 4114
Size: 7094
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BcgI BmtI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NcoI NheI NotI NruI PacI PasI PfoI
PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI