Restriction map for
pF1KA1209
(Circular) (Six-base) MAPSORT of: pF1KA1209.seq Check: 3610 from: 1 to: 7445
pF1KA1209 7445 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 4483 4483
Size: 7445
Acc65I G'GTAC_C
Cuts at: 4461 4461
Size: 7445
AclI AA'CG_TT
Cuts at: 7285 7285
Size: 7445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 951 1413 1772 5270 5702 6055 6717 298
Size: 653 462 359 3498 432 353 662 1026
Fragments arranged by size:
3498 1026 662 653 462 432 359 353
AfeI AGC'GCT
Cuts at: 3801 3801
Size: 7445
AflIII A'CryG_T
Cuts at: 1352 4797 6597 6878 1352
Size: 3445 1800 281 1919
Fragments arranged by size:
3445 1919 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 3600 3600
Size: 7445
AleI CACnn'nnGTG
Cuts at: 2989 2989
Size: 7445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 161 499 1408 4261 161
Size: 338 909 2853 3345
Fragments arranged by size:
3345 2853 909 338
AlwNI CAG_nnn'CTG
Cuts at: 3706 3903 3906 4750 6188 3706
Size: 197 3 844 1438 4963
Fragments arranged by size:
4963 1438 844 197 3
ApaI G_GGCC'C
Cuts at: 3371 3371
Size: 7445
ApaLI G'TGCA_C
Cuts at: 6283 6283
Size: 7445
ApoI r'AATT_y
Cuts at: 644 961 3443 4044 4408 4449 6798 7054 644
Size: 317 2482 601 364 41 2349 256 1035
Fragments arranged by size:
2482 2349 1035 601 364 317 256 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 7445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7445
AvaI C'yCGr_G
Cuts at: 4465 4465
Size: 7445
BamHI G'GATC_C
Cuts at: 4470 4470
Size: 7445
BanI G'GyrC_C
Cuts at: 191 576 2440 3379 4461 4875 5123 5158 191
Size: 385 1864 939 1082 414 248 35 2478
Fragments arranged by size:
2478 1864 1082 939 414 385 248 35
BanII G_rGCy'C
Cuts at: 293 610 1869 2419 2525 3371 4459 5489 293
Size: 317 1259 550 106 846 1088 1030 2249
Fragments arranged by size:
2249 1259 1088 1030 846 550 317 106
BbeI G_GCGC'C
Cuts at: 4879 5127 4879
Size: 248 7197
BbsI GAAGACnn'nnnn_
Cuts at: 286 1428 1729 1778 2840 3047 286
Size: 1142 301 49 1062 207 4684
Fragments arranged by size:
4684 1142 1062 301 207 49
BciVI GTATCCnnnnn_n'
Cuts at: 5343 6399 7445 5343
Size: 1056 1046 5343
Fragments arranged by size:
5343 1056 1046
BclI T'GATC_A
Cuts at: 1841 3848 4965 1841
Size: 2007 1117 4321
Fragments arranged by size:
4321 2007 1117
BglI GCCn_nnn'nGGC
Cuts at: 404 2895 404
Size: 2491 4954
BglII A'GATC_T
Cuts at: 1992 3999 4960 6997 1992
Size: 2007 961 2037 2440
Fragments arranged by size:
2440 2037 2007 961
BlpI GC'TnA_GC
Cuts at: 1423 2306 2923 4557 1423
Size: 883 617 1634 4311
Fragments arranged by size:
4311 1634 883 617
Bme1580I G_kGCm'C
Cuts at: 194 579 3371 3985 5070 5163 6287 194
Size: 385 2792 614 1085 93 1124 1352
Fragments arranged by size:
2792 1352 1124 1085 614 385 93
BmgBI CAC'GTC
Cuts at: 2934 2934
Size: 7445
BmrI ACTGGGnnnn_n'
Cuts at: 885 2726 3396 3822 4657 4738 5073 7217 885
Size: 1841 670 426 835 81 335 2144 1113
Fragments arranged by size:
2144 1841 1113 835 670 426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2993 5812 2869
Size: 124 2819 4502
Fragments arranged by size:
4502 2819 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1552 2064 2617 2638 3301 3307 466
Size: 1086 512 553 21 663 6 4604
Fragments arranged by size:
4604 1086 663 553 512 21 6
Bpu10I CC'TnA_GC
Cuts at: 1559 2918 1559
Size: 1359 6086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 1027 1282 3455 3618 4621 5500 6214 6512
Size: 773 255 2173 163 1003 879 714 298
Cuts at: 6512 254
Size: 1187
Fragments arranged by size:
2173 1187 1003 879 773 714 298 255
163
BsaI GGTCTCn'nnnn_
Cuts at: 2068 2840 7149 2068
Size: 772 4309 2364
Fragments arranged by size:
4309 2364 772
BsaAI yAC'GTr
Cuts at: 4798 4798
Size: 7445
BsaBI GATnn'nnATC
Cuts at: 6 1958 4959 6996 6
Size: 1952 3001 2037 455
Fragments arranged by size:
3001 2037 1952 455
BsaHI Gr'CG_yC
Cuts at: 4876 5124 5826 7322 4876
Size: 248 702 1496 4999
Fragments arranged by size:
4999 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 4634 5155 5833 6244 6391 6665 1187
Size: 3447 521 678 411 147 274 1967
Fragments arranged by size:
3447 1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2124 2154 2124
Size: 30 7415
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 1977 2221 2512 2971 607
Size: 1370 244 291 459 5081
Fragments arranged by size:
5081 1370 459 291 244
BseYI C'CCAG_C
Cuts at: 1484 1778 1915 2119 2541 3458 4870 5628 6293
Size: 294 137 204 422 917 1412 758 665
Cuts at: 6293 6701 1484
Size: 408 2228
Fragments arranged by size:
2228 1412 917 758 665 422 408 294
204 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2574 2884 3730 1495
Size: 1079 310 846 5210
Fragments arranged by size:
5210 1079 846 310
BsiEI CG_ry'CG
Cuts at: 104 590 4987 5033 6263 104
Size: 486 4397 46 1230 1286
Fragments arranged by size:
4397 1286 1230 486 46
BsiHKAI G_wGCw'C
Cuts at: 293 1181 1869 2371 2584 2914 4459 5237 5427
Size: 888 688 502 213 330 1545 778 190
Cuts at: 5427 6287 293
Size: 860 1451
Fragments arranged by size:
1545 1451 888 860 778 688 502 330
213 190
BsmI GAATG_Cn'
Cuts at: 3540 4248 3540
Size: 708 6737
BsmBI CGTCTCn'nnnn_
Cuts at: 1805 2857 1805
Size: 1052 6393
Bsp1286I G_dGCh'C
Cuts at: 194 293 579 610 1181 1869 2371 2419 2525
Size: 99 286 31 571 688 502 48 106
Cuts at: 2525 2584 2914 3371 3985 4459 5070 5163 5237
Size: 59 330 457 614 474 611 93 74
Cuts at: 5237 5427 5489 6287 194
Size: 190 62 798 1352
Fragments arranged by size:
1352 798 688 614 611 571 502 474
457 330 286 190 106 99 93 74
62 59 48 31
BspHI T'CATG_A
Cuts at: 5877 5877
Size: 7445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4495 4709 5011 5392 4495
Size: 214 302 381 6548
Fragments arranged by size:
6548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1299 1422 1858 5737 5791 7275 7440 50
Size: 1249 123 436 3879 54 1484 165 55
Fragments arranged by size:
3879 1484 1249 436 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 4243 5357 4243
Size: 1114 6331
BsrFI r'CCGG_y
Cuts at: 3080 3297 4281 5443 5624 3080
Size: 217 984 1162 181 4901
Fragments arranged by size:
4901 1162 984 217 181
BsrGI T'GTAC_A
Cuts at: 669 669
Size: 7445
BssSI C'ACGA_G
Cuts at: 603 1174 5716 6424 603
Size: 571 4542 708 1624
Fragments arranged by size:
4542 1624 708 571
BstAPI GCAn_nnn'nTGC
Cuts at: 198 2135 3903 4822 198
Size: 1937 1768 919 2821
Fragments arranged by size:
2821 1937 1768 919
BstBI TT'CG_AA
Cuts at: 5806 5806
Size: 7445
BstXI CCAn_nnnn'nTGG
Cuts at: 444 3523 444
Size: 3079 4366
BstYI r'GATC_y
Cuts at: 1 1771 1992 2529 3999 4470 4937 4960 5295
Size: 1770 221 537 1470 471 467 23 335
Cuts at: 5295 5541 5847 5859 5945 6997 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1770 1470 1052 537 471 467 449 335
306 246 221 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1622 2626 3487 1622
Size: 1004 861 5580
Fragments arranged by size:
5580 1004 861
BtgI C'CryG_G
Cuts at: 107 357 503 917 3516 107
Size: 250 146 414 2599 4036
Fragments arranged by size:
4036 2599 414 250 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5395 5576 5739 5395
Size: 181 163 7101
Fragments arranged by size:
7101 181 163
BtsI GCAGTG_nn'
Cuts at: 559 2121 4688 559
Size: 1562 2567 3316
Fragments arranged by size:
3316 2567 1562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 899 934 4184 4219 899
Size: 35 3250 35 4125
Fragments arranged by size:
4125 3250 35 35
DraI TTT'AAA
Cuts at: 680 3266 4444 680
Size: 2586 1178 3681
Fragments arranged by size:
3681 2586 1178
DrdI GACnn_nn'nnGTC
Cuts at: 310 5151 6495 310
Size: 4841 1344 1260
Fragments arranged by size:
4841 1344 1260
EaeI y'GGCC_r
Cuts at: 599 839 956 2403 3131 3299 5030 5204 5595
Size: 240 117 1447 728 168 1731 174 391
Cuts at: 5595 5622 6967 599
Size: 27 1345 1077
Fragments arranged by size:
1731 1447 1345 1077 728 391 240 174
168 117 27
EagI C'GGCC_G
Cuts at: 5030 5030
Size: 7445
EarI CTCTTCn'nnn_
Cuts at: 127 348 866 1312 1318 1336 2989 3344 3683
Size: 221 518 446 6 18 1653 355 339
Cuts at: 3683 5468 5678 127
Size: 1785 210 1894
Fragments arranged by size:
1894 1785 1653 518 446 355 339 221
210 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 144 5653 6395 6541 7255 144
Size: 5509 742 146 714 334
Fragments arranged by size:
5509 742 714 334 146
EcoICRI GAG'CTC
Cuts at: 291 1867 4457 291
Size: 1576 2590 3279
Fragments arranged by size:
3279 2590 1576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 466 951 1413 1552 1772 2064 2617 2638
Size: 168 485 462 139 220 292 553 21
Cuts at: 2638 3301 3307 5270 5702 6055 6717 298
Size: 663 6 1963 432 353 662 1026
Fragments arranged by size:
1963 1026 663 662 553 485 462 432
353 292 220 168 139 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1084 1625 2147 3698 1084
Size: 541 522 1551 4831
Fragments arranged by size:
4831 1551 541 522
EcoO109I rG'GnC_Cy
Cuts at: 1010 3367 3824 4584 1010
Size: 2357 457 760 3871
Fragments arranged by size:
3871 2357 760 457
EcoRI G'AATT_C
Cuts at: 4449 4449
Size: 7445
EcoRV GAT'ATC
Cuts at: 780 3610 780
Size: 2830 4615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 5464 1594
Size: 3870 3575
FspI TGC'GCA
Cuts at: 5226 5226
Size: 7445
HaeII r_GCGC'y
Cuts at: 2943 3803 4879 5127 6357 7128 2943
Size: 860 1076 248 1230 771 3260
Fragments arranged by size:
3260 1230 1076 860 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1756 1788 1803 1835 1984 2016 2521 2553 2761
Size: 32 15 32 149 32 505 32 208
Cuts at: 2761 2793 3586 3618 5287 5317 5319 5349 1756
Size: 32 793 32 1669 30 2 30 3852
Fragments arranged by size:
3852 1669 793 505 208 149 32 32
32 32 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 2240 4287 4484 2240
Size: 2047 197 5201
Fragments arranged by size:
5201 2047 197
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 7445
KasI G'GCGC_C
Cuts at: 4875 5123 4875
Size: 248 7197
KpnI G_GTAC'C
Cuts at: 4465 4465
Size: 7445
MfeI C'AATT_G
Cuts at: 3649 3649
Size: 7445
MluI A'CGCG_T
Cuts at: 6878 6878
Size: 7445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4133 4139 6204 6388 3766
Size: 367 6 2065 184 4823
Fragments arranged by size:
4823 2065 367 184 6
MscI TGG'CCA
Cuts at: 841 958 2405 3133 5206 841
Size: 117 1447 728 2073 3080
Fragments arranged by size:
3080 2073 1447 728 117
MslI CAynn'nnrTG
Cuts at: 1154 1396 2264 2989 3521 5434 5561 1154
Size: 242 868 725 532 1913 127 3038
Fragments arranged by size:
3038 1913 868 725 532 242 127
MspA1I CmG'CkG
Cuts at: 220 1035 1782 2045 3903 4235 4556 4870 5230
Size: 815 747 263 1858 332 321 314 360
Cuts at: 5230 6014 6259 220
Size: 784 245 1406
Fragments arranged by size:
1858 1406 815 784 747 360 332 321
314 263 245
NaeI GCC'GGC
Cuts at: 3299 5626 3299
Size: 2327 5118
NarI GG'CG_CC
Cuts at: 4876 5124 4876
Size: 248 7197
NcoI C'CATG_G
Cuts at: 107 357 3516 107
Size: 250 3159 4036
Fragments arranged by size:
4036 3159 250
NdeI CA'TA_TG
Cuts at: 2051 2051
Size: 7445
NgoMIV G'CCGG_C
Cuts at: 3297 5624 3297
Size: 2327 5118
NspI r_CATG'y
Cuts at: 2138 4498 4998 6601 2138
Size: 2360 500 1603 2982
Fragments arranged by size:
2982 2360 1603 500
PasI CC'CwG_GG
Cuts at: 735 735
Size: 7445
PciI A'CATG_T
Cuts at: 6597 6597
Size: 7445
PmeI GTTT'AAAC
Cuts at: 4444 4444
Size: 7445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5891 5923 5891
Size: 32 7413
PpuMI rG'GwC_Cy
Cuts at: 1010 3824 1010
Size: 2814 4631
PspOMI G'GGCC_C
Cuts at: 3367 3367
Size: 7445
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 215 247 215
Size: 32 7413
PstI C_TGCA'G
Cuts at: 488 1463 2180 4234 4492 5177 488
Size: 975 717 2054 258 685 2756
Fragments arranged by size:
2756 2054 975 717 685 258
PvuI CG_AT'CG
Cuts at: 104 590 104
Size: 486 6959
PvuII CAG'CTG
Cuts at: 220 1782 2045 3903 4870 5230 220
Size: 1562 263 1858 967 360 2435
Fragments arranged by size:
2435 1858 1562 967 360 263
RsrII CG'GwC_CG
Cuts at: 1185 5640 1185
Size: 4455 2990
SacI G_AGCT'C
Cuts at: 293 1869 4459 293
Size: 1576 2590 3279
Fragments arranged by size:
3279 2590 1576
SalI G'TCGA_C
Cuts at: 4482 4482
Size: 7445
SapI GCTCTTCn'nnn_
Cuts at: 1312 2989 5468 5678 1312
Size: 1677 2479 210 3079
Fragments arranged by size:
3079 2479 1677 210
SbfI CC_TGCA'GG
Cuts at: 4492 4492
Size: 7445
ScaI AGT'ACT
Cuts at: 3639 6962 3639
Size: 3323 4122
SfcI C'TryA_G
Cuts at: 33 484 1121 1459 2176 3549 4230 4488 5173
Size: 451 637 338 717 1373 681 258 685
Cuts at: 5173 6141 6332 33
Size: 968 191 1146
Fragments arranged by size:
1373 1146 968 717 685 681 637 451
338 258 191
SfoI GGC'GCC
Cuts at: 4877 5125 4877
Size: 248 7197
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7445
SmaI CCC'GGG
Cuts at: 4467 4467
Size: 7445
SmlI C'TyrA_G
Cuts at: 269 1042 1261 3470 3597 4600 5515 6229 6491
Size: 773 219 2209 127 1003 915 714 262
Cuts at: 6491 269
Size: 1223
Fragments arranged by size:
2209 1223 1003 915 773 714 262 219
127
SpeI A'CTAG_T
Cuts at: 67 2155 67
Size: 2088 5357
SphI G_CATG'C
Cuts at: 4498 4998 4498
Size: 500 6945
SspI AAT'ATT
Cuts at: 4386 4386
Size: 7445
StyI C'CwwG_G
Cuts at: 107 357 910 3516 3779 4579 4933 107
Size: 250 553 2606 263 800 354 2619
Fragments arranged by size:
2619 2606 800 553 354 263 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 320 558 594 757 1900 5028 5161 5620 5829
Size: 238 36 163 1143 3128 133 459 209
Cuts at: 5829 320
Size: 1936
Fragments arranged by size:
3128 1936 1143 459 238 209 163 133
36
TatI w'GTAC_w
Cuts at: 669 3230 3637 6960 669
Size: 2561 407 3323 1154
Fragments arranged by size:
3323 2561 1154 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 891 923 1730 1762 2124 2156 6962
Size: 32 712 32 807 32 362 32 4806
Cuts at: 6962 6994 147
Size: 32 598
Fragments arranged by size:
4806 807 712 598 362 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 5242 2260
Size: 2982 4463
XbaI T'CTAG_A
Cuts at: 1759 4476 1759
Size: 2717 4728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3514 3520 4940 3514
Size: 6 1420 6019
Fragments arranged by size:
6019 1420 6
XmaI C'CCGG_G
Cuts at: 4465 4465
Size: 7445
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 7445
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BspEI BssHII BstEII
BstZ17I ClaI DraIII FseI FspAI HindIII NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI