Restriction map for
pF1KA1193
(Circular) (Six-base) MAPSORT of: pF1KA1193.seq Check: 1599 from: 1 to: 4748
pF1KA1193 4748 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 1786 1786
Size: 4748
Acc65I G'GTAC_C
Cuts at: 1764 1764
Size: 4748
AclI AA'CG_TT
Cuts at: 4588 4588
Size: 4748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 3005 3752 4020 2573
Size: 432 747 268 3301
Fragments arranged by size:
3301 747 432 268
AfeI AGC'GCT
Cuts at: 1153 1153
Size: 4748
AflIII A'CryG_T
Cuts at: 2100 3204 4181 2100
Size: 1104 977 2667
Fragments arranged by size:
2667 1104 977
AgeI A'CCGG_T
Cuts at: 3136 3136
Size: 4748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 376 548 376
Size: 172 4576
AlwNI CAG_nnn'CTG
Cuts at: 918 1233 1407 2053 3620 918
Size: 315 174 646 1567 2046
Fragments arranged by size:
2046 1567 646 315 174
ApaLI G'TGCA_C
Cuts at: 3518 3518
Size: 4748
ApoI r'AATT_y
Cuts at: 1752 4101 4357 1752
Size: 2349 256 2143
Fragments arranged by size:
2349 2143 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
AvaI C'yCGr_G
Cuts at: 160 297 1696 1768 160
Size: 137 1399 72 3140
Fragments arranged by size:
3140 1399 137 72
BamHI G'GATC_C
Cuts at: 1581 1773 1581
Size: 192 4556
BanI G'GyrC_C
Cuts at: 302 1764 2178 2426 2461 302
Size: 1462 414 248 35 2589
Fragments arranged by size:
2589 1462 414 248 35
BanII G_rGCy'C
Cuts at: 223 1004 1349 1715 1762 2792 223
Size: 781 345 366 47 1030 2179
Fragments arranged by size:
2179 1030 781 366 345 47
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4500
BbsI GAAGACnn'nnnn_
Cuts at: 626 782 626
Size: 156 4592
BbvCI CC'TCA_GC
Cuts at: 727 875 1573 727
Size: 148 698 3902
Fragments arranged by size:
3902 698 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 124 158 477 511 124
Size: 34 319 34 4361
Fragments arranged by size:
4361 319 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2646 3407 4748 2646
Size: 761 1341 2646
Fragments arranged by size:
2646 1341 761
BclI T'GATC_A
Cuts at: 2268 2268
Size: 4748
BglII A'GATC_T
Cuts at: 761 2263 4300 761
Size: 1502 2037 1209
Fragments arranged by size:
2037 1502 1209
BlpI GC'TnA_GC
Cuts at: 1860 1860
Size: 4748
Bme1580I G_kGCm'C
Cuts at: 178 307 1690 2373 2466 3522 178
Size: 129 1383 683 93 1056 1404
Fragments arranged by size:
1404 1383 1056 683 129 93
BmgBI CAC'GTC
Cuts at: 441 1656 441
Size: 1215 3533
BmrI ACTGGGnnnn_n'
Cuts at: 1301 1960 2041 2376 4520 1301
Size: 659 81 335 2144 1529
Fragments arranged by size:
2144 1529 659 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 138 312 617 651 681 1018 3115 138
Size: 174 305 34 30 337 2097 1771
Fragments arranged by size:
2097 1771 337 305 174 34 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 349 1542 245
Size: 104 1193 3451
Fragments arranged by size:
3451 1193 104
Bpu10I CC'TnA_GC
Cuts at: 727 875 1573 727
Size: 148 698 3902
Fragments arranged by size:
3902 698 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 2803 3295 3593 1924
Size: 879 492 298 3079
Fragments arranged by size:
3079 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 156 221 1342 4452 156
Size: 65 1121 3110 452
Fragments arranged by size:
3110 1121 452 65
BsaAI yAC'GTr
Cuts at: 1685 2101 1685
Size: 416 4332
BsaBI GATnn'nnATC
Cuts at: 6 2262 4299 6
Size: 2256 2037 455
Fragments arranged by size:
2256 2037 455
BsaHI Gr'CG_yC
Cuts at: 400 1502 2179 2427 3129 4625 400
Size: 1102 677 248 702 1496 523
Fragments arranged by size:
1496 1102 702 677 523 248
BsaWI w'CCGG_w
Cuts at: 590 1207 1491 1584 1937 2458 3136 3410 3557
Size: 617 284 93 353 521 678 274 147
Cuts at: 3557 3968 590
Size: 411 1370
Fragments arranged by size:
1370 678 617 521 411 353 284 274
147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 123 153 328 358 556 586 817 847 881
Size: 30 175 30 198 30 231 30 34
Cuts at: 881 911 1702 1732 123
Size: 30 791 30 3139
Fragments arranged by size:
3139 791 231 198 175 34 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 111 304 337 636 673 838 967 1036 1603
Size: 193 33 299 37 165 129 69 567
Cuts at: 1603 111
Size: 3256
Fragments arranged by size:
3256 567 299 193 165 129 69 37
33
BseYI C'CCAG_C
Cuts at: 126 796 1169 1182 1446 1461 1602 2173 2931
Size: 670 373 13 264 15 141 571 758
Cuts at: 2931 3508 4004 126
Size: 577 496 870
Fragments arranged by size:
870 758 670 577 571 496 373 264
141 15 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1047 721
Size: 326 4422
BsiEI CG_ry'CG
Cuts at: 104 1263 1331 2290 2336 3544 104
Size: 1159 68 959 46 1208 1308
Fragments arranged by size:
1308 1208 1159 959 68 46
BsiHKAI G_wGCw'C
Cuts at: 167 1043 1762 2540 2730 3522 167
Size: 876 719 778 190 792 1393
Fragments arranged by size:
1393 876 792 778 719 190
BsmBI CGTCTCn'nnnn_
Cuts at: 514 514
Size: 4748
Bsp1286I G_dGCh'C
Cuts at: 167 178 223 307 1004 1043 1349 1690 1715
Size: 11 45 84 697 39 306 341 25
Cuts at: 1715 1762 2373 2466 2540 2730 2792 3522 167
Size: 47 611 93 74 190 62 730 1393
Fragments arranged by size:
1393 730 697 611 341 306 190 93
84 74 62 47 45 39 25 11
BspEI T'CCGG_A
Cuts at: 1207 1491 1584 3968 1207
Size: 284 93 2384 1987
Fragments arranged by size:
2384 1987 284 93
BspHI T'CATG_A
Cuts at: 3924 3924
Size: 4748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 743 1747 1798 2012 2314 2695 743
Size: 1004 51 214 302 381 2796
Fragments arranged by size:
2796 1004 381 302 214 51
BsrBI CCG'CTC
Cuts at: 50 3040 3094 4578 4743 50
Size: 2990 54 1484 165 55
Fragments arranged by size:
2990 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2660 2660
Size: 4748
BsrFI r'CCGG_y
Cuts at: 746 1330 2746 2927 3136 746
Size: 584 1416 181 209 2358
Fragments arranged by size:
2358 1416 584 209 181
BsrGI T'GTAC_A
Cuts at: 833 833
Size: 4748
BssSI C'ACGA_G
Cuts at: 565 859 3019 3377 565
Size: 294 2160 358 1936
Fragments arranged by size:
2160 1936 358 294
BstAPI GCAn_nnn'nTGC
Cuts at: 2125 2125
Size: 4748
BstBI TT'CG_AA
Cuts at: 3109 3109
Size: 4748
BstEII G'GTnAC_C
Cuts at: 1623 1623
Size: 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 1566 1566
Size: 4748
BstYI r'GATC_y
Cuts at: 1 251 598 761 1305 1581 1773 2240 2263
Size: 250 347 163 544 276 192 467 23
Cuts at: 2263 2598 2844 3856 3942 3954 4300 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 544 467 449 347 346 335 276
250 246 192 163 86 23 12
BtgI C'CryG_G
Cuts at: 107 215 1214 1520 107
Size: 108 999 306 3335
Fragments arranged by size:
3335 999 306 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2698 2879 3042 2698
Size: 181 163 4404
Fragments arranged by size:
4404 181 163
BtsI GCAGTG_nn'
Cuts at: 212 1484 1991 212
Size: 1272 507 2969
Fragments arranged by size:
2969 1272 507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 188 223 188
Size: 35 4713
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4748
DrdI GACnn_nn'nnGTC
Cuts at: 799 2454 3312 799
Size: 1655 858 2235
Fragments arranged by size:
2235 1655 858
EaeI y'GGCC_r
Cuts at: 866 1260 1328 1454 1517 1670 2333 2507 2898
Size: 394 68 126 63 153 663 174 391
Cuts at: 2898 2925 4270 866
Size: 27 1345 1344
Fragments arranged by size:
1345 1344 663 394 391 174 153 126
68 63 27
EagI C'GGCC_G
Cuts at: 1260 1328 2333 1260
Size: 68 1005 3675
Fragments arranged by size:
3675 1005 68
EarI CTCTTCn'nnn_
Cuts at: 506 512 586 642 674 1013 2771 2981 506
Size: 6 74 56 32 339 1758 210 2273
Fragments arranged by size:
2273 1758 339 210 74 56 32 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2956 3266 3412 4558 127
Size: 2829 310 146 1146 317
Fragments arranged by size:
2829 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1760 1760
Size: 4748
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 349 1542 2573 3005 3752 4020 245
Size: 104 1193 1031 432 747 268 973
Fragments arranged by size:
1193 1031 973 747 432 268 104
EcoNI CCTnn'n_nnAGG
Cuts at: 1078 1537 1078
Size: 459 4289
EcoO109I rG'GnC_Cy
Cuts at: 706 792 957 1230 1887 706
Size: 86 165 273 657 3567
Fragments arranged by size:
3567 657 273 165 86
EcoRI G'AATT_C
Cuts at: 1752 1752
Size: 4748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4748
FspI TGC'GCA
Cuts at: 1096 1300 2529 1096
Size: 204 1229 3315
Fragments arranged by size:
3315 1229 204
FspAI rTGC'GCAy
Cuts at: 1096 1300 1096
Size: 204 4544
HaeII r_GCGC'y
Cuts at: 919 1155 2182 2430 3452 4431 919
Size: 236 1027 248 1022 979 1236
Fragments arranged by size:
1236 1027 1022 979 248 236
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 241 273 328 360 511 543 1450
Size: 32 86 32 55 32 151 32 907
Cuts at: 1450 1482 1702 1734 2590 2620 2622 2652 123
Size: 32 220 32 856 30 2 30 2219
Fragments arranged by size:
2219 907 856 220 151 86 55 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1787 1787
Size: 4748
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4500
KpnI G_GTAC'C
Cuts at: 1768 1768
Size: 4748
MluI A'CGCG_T
Cuts at: 4181 4181
Size: 4748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 599 674 1127 3419 3603 599
Size: 75 453 2292 184 1744
Fragments arranged by size:
2292 1744 453 184 75
MscI TGG'CCA
Cuts at: 2509 2509
Size: 4748
MslI CAynn'nnrTG
Cuts at: 2737 2864 2737
Size: 127 4621
MspA1I CmG'CkG
Cuts at: 783 1407 1423 1480 1577 1859 2173 2533 3546
Size: 624 16 57 97 282 314 360 1013
Cuts at: 3546 3791 783
Size: 245 1740
Fragments arranged by size:
1740 1013 624 360 314 282 245 97
57 16
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 4748
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4500
NcoI C'CATG_G
Cuts at: 107 215 107
Size: 108 4640
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 4748
NspI r_CATG'y
Cuts at: 363 1801 2301 3208 363
Size: 1438 500 907 1903
Fragments arranged by size:
1903 1438 907 500
PasI CC'CwG_GG
Cuts at: 178 178
Size: 4748
PciI A'CATG_T
Cuts at: 3204 3204
Size: 4748
PflMI CCAn_nnn'nTGG
Cuts at: 1233 1407 1233
Size: 174 4574
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4748
PmlI CAC'GTG
Cuts at: 1685 1685
Size: 4748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4716
PpuMI rG'GwC_Cy
Cuts at: 706 792 1230 706
Size: 86 438 4224
Fragments arranged by size:
4224 438 86
PshAI GACnn'nnGTC
Cuts at: 1371 1371
Size: 4748
PspXI vC'TCGA_Gb
Cuts at: 160 297 160
Size: 137 4611
PstI C_TGCA'G
Cuts at: 1795 2480 1795
Size: 685 4063
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4748
PvuII CAG'CTG
Cuts at: 783 1407 1480 2173 2533 783
Size: 624 73 693 360 2998
Fragments arranged by size:
2998 693 624 360 73
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4748
SacI G_AGCT'C
Cuts at: 1762 1762
Size: 4748
SalI G'TCGA_C
Cuts at: 1785 1785
Size: 4748
SanDI GG'GwC_CC
Cuts at: 792 792
Size: 4748
SapI GCTCTTCn'nnn_
Cuts at: 642 2771 2981 642
Size: 2129 210 2409
Fragments arranged by size:
2409 2129 210
SbfI CC_TGCA'GG
Cuts at: 1795 1795
Size: 4748
ScaI AGT'ACT
Cuts at: 1126 4265 1126
Size: 3139 1609
SfcI C'TryA_G
Cuts at: 33 267 1791 2476 3469 3660 33
Size: 234 1524 685 993 191 1121
Fragments arranged by size:
1524 1121 993 685 234 191
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4500
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
SmaI CCC'GGG
Cuts at: 1698 1770 1698
Size: 72 4676
SmlI C'TyrA_G
Cuts at: 160 297 1903 2818 3310 3572 160
Size: 137 1606 915 492 262 1336
Fragments arranged by size:
1606 1336 915 492 262 137
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4748
SphI G_CATG'C
Cuts at: 1801 2301 1801
Size: 500 4248
StuI AGG'CCT
Cuts at: 118 295 571 118
Size: 177 276 4295
Fragments arranged by size:
4295 276 177
StyI C'CwwG_G
Cuts at: 107 215 317 1510 1538 1882 2236 107
Size: 108 102 1193 28 344 354 2619
Fragments arranged by size:
2619 1193 354 344 108 102 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 2331 2464 2923 3132 1611
Size: 720 133 459 209 3227
Fragments arranged by size:
3227 720 459 209 133
TatI w'GTAC_w
Cuts at: 833 1124 4263 833
Size: 291 3139 1318
Fragments arranged by size:
3139 1318 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 556 588 881 913 4265 4297 556
Size: 32 293 32 3352 32 1007
Fragments arranged by size:
3352 1007 293 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2545 2545
Size: 4748
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 1234 1512 2243 324
Size: 910 278 731 2829
Fragments arranged by size:
2829 910 731 278
XhoI C'TCGA_G
Cuts at: 160 297 160
Size: 137 4611
XmaI C'CCGG_G
Cuts at: 1696 1768 1696
Size: 72 4676
XmnI GAAnn'nnTTC
Cuts at: 337 1663 337
Size: 1326 3422
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmtI BsiWI BsmI BssHII
BstZ17I Bsu36I ClaI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PsiI PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI