Restriction map for
pF1KA1180
(Circular) (Six-base) MAPSORT of: pF1KA1180.seq Check: 1703 from: 1 to: 4169
pF1KA1180 4169 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 356 1185 356
Size: 829 3340
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 901 956 1994 2426 3173 3441 600
Size: 301 55 1038 432 747 268 1328
Fragments arranged by size:
1328 1038 747 432 301 268 55
AflIII A'CryG_T
Cuts at: 716 1521 2625 3602 716
Size: 805 1104 977 1283
Fragments arranged by size:
1283 1104 977 805
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 401 401
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 895 1474 3041 895
Size: 579 1567 2023
Fragments arranged by size:
2023 1567 579
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 1173 3522 3778 1173
Size: 2349 256 1564
Fragments arranged by size:
2349 1564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 1189 1189
Size: 4169
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 356 448 762 1185 1599 1847 1882 356
Size: 92 314 423 414 248 35 2643
Fragments arranged by size:
2643 423 414 314 248 92 35
BanII G_rGCy'C
Cuts at: 181 1183 2213 181
Size: 1002 1030 2137
Fragments arranged by size:
2137 1030 1002
BbeI G_GCGC'C
Cuts at: 452 1603 1851 452
Size: 1151 248 2770
Fragments arranged by size:
2770 1151 248
BbvCI CC'TCA_GC
Cuts at: 824 1017 824
Size: 193 3976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4135
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 1281 1281
Size: 4169
Bme1580I G_kGCm'C
Cuts at: 767 1016 1794 1887 2943 767
Size: 249 778 93 1056 1993
Fragments arranged by size:
1993 1056 778 249 93
BmrI ACTGGGnnnn_n'
Cuts at: 561 1381 1462 1797 3941 561
Size: 820 81 335 2144 789
Fragments arranged by size:
2144 820 789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 864 2536 864
Size: 1672 2497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 917
Size: 4169
Bpu10I CC'TnA_GC
Cuts at: 824 1017 824
Size: 193 3976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2224 2716 3014 1345
Size: 879 492 298 2500
Fragments arranged by size:
2500 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 728 3873 728
Size: 3145 1024
BsaAI yAC'GTr
Cuts at: 309 1522 309
Size: 1213 2956
BsaBI GATnn'nnATC
Cuts at: 6 436 1683 3720 6
Size: 430 1247 2037 455
Fragments arranged by size:
2037 1247 455 430
BsaHI Gr'CG_yC
Cuts at: 337 449 1600 1848 2550 4046 337
Size: 112 1151 248 702 1496 460
Fragments arranged by size:
1496 1151 702 460 248 112
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 446 476 995 1025 446
Size: 30 519 30 3590
Fragments arranged by size:
3590 519 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 640 1019 640
Size: 379 3790
BseYI C'CCAG_C
Cuts at: 119 198 397 1128 1594 2352 2929 3425 119
Size: 79 199 731 466 758 577 496 863
Fragments arranged by size:
863 758 731 577 496 466 199 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 673 1086 427
Size: 246 413 3510
Fragments arranged by size:
3510 413 246
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 605 1183 1961 2151 2943 605
Size: 578 778 190 792 1831
Fragments arranged by size:
1831 792 778 578 190
Bsp1286I G_dGCh'C
Cuts at: 181 605 767 1016 1183 1794 1887 1961 2151
Size: 424 162 249 167 611 93 74 190
Cuts at: 2151 2213 2943 181
Size: 62 730 1407
Fragments arranged by size:
1407 730 611 424 249 190 167 162
93 74 62
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 707 1219 1433 1735 2116 707
Size: 512 214 302 381 2760
Fragments arranged by size:
2760 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 971 2081 971
Size: 1110 3059
BsrFI r'CCGG_y
Cuts at: 662 749 2167 2348 2557 662
Size: 87 1418 181 209 2274
Fragments arranged by size:
2274 1418 209 181 87
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 4169
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 1077 1077
Size: 4169
BstYI r'GATC_y
Cuts at: 1 1194 1661 1684 2019 2265 3277 3363 3375
Size: 1193 467 23 335 246 1012 86 12
Cuts at: 3375 3721 1
Size: 346 449
Fragments arranged by size:
1193 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 350 350
Size: 4169
BtgI C'CryG_G
Cuts at: 371 1148 371
Size: 777 3392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 1016 1049 1412 1016
Size: 33 363 3773
Fragments arranged by size:
3773 363 33
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DraIII CAC_nnn'GTG
Cuts at: 297 297
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 467 1754 1928 2319 2346 3691 467
Size: 1287 174 391 27 1345 945
Fragments arranged by size:
1345 1287 945 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 619 712 2192 2402 619
Size: 93 1480 210 2386
Fragments arranged by size:
2386 1480 210 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 901 917 956 1994 2426 3173 3441 600
Size: 301 16 39 1038 432 747 268 1328
Fragments arranged by size:
1328 1038 747 432 301 268 39 16
EcoNI CCTnn'n_nnAGG
Cuts at: 499 499
Size: 4169
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 452 1603 1851 2873 3852 452
Size: 1151 248 1022 979 769
Fragments arranged by size:
1151 1022 979 769 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2011 2041 2043 2073 2011
Size: 30 2 30 4107
Fragments arranged by size:
4107 30 30 2
HincII GTy'rAC
Cuts at: 1140 1208 1140
Size: 68 4101
KasI G'GCGC_C
Cuts at: 448 1599 1847 448
Size: 1151 248 2770
Fragments arranged by size:
2770 1151 248
KpnI G_GTAC'C
Cuts at: 360 1189 360
Size: 829 3340
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 469 1930 469
Size: 1461 2708
MslI CAynn'nnrTG
Cuts at: 311 442 1153 2158 2285 311
Size: 131 711 1005 127 2195
Fragments arranged by size:
2195 1005 711 131 127
MspA1I CmG'CkG
Cuts at: 381 785 823 1280 1594 1954 2967 3212 381
Size: 404 38 457 314 360 1013 245 1338
Fragments arranged by size:
1338 1013 457 404 360 314 245 38
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 449 1600 1848 449
Size: 1151 248 2770
Fragments arranged by size:
2770 1151 248
NcoI C'CATG_G
Cuts at: 371 1148 371
Size: 777 3392
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 166 276 720 1222 1722 2629 166
Size: 110 444 502 500 907 1706
Fragments arranged by size:
1706 907 502 500 444 110
PciI A'CATG_T
Cuts at: 716 2625 716
Size: 1909 2260
PflMI CCAn_nnn'nTGG
Cuts at: 222 1149 222
Size: 927 3242
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PmlI CAC'GTG
Cuts at: 309 309
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PstI C_TGCA'G
Cuts at: 704 1216 1901 704
Size: 512 685 2972
Fragments arranged by size:
2972 685 512
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 381 1594 1954 381
Size: 1213 360 2596
Fragments arranged by size:
2596 1213 360
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 712 2192 2402 712
Size: 1480 210 2479
Fragments arranged by size:
2479 1480 210
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SexAI A'CCwGG_T
Cuts at: 494 494
Size: 4169
SfcI C'TryA_G
Cuts at: 33 700 1212 1897 2890 3081 33
Size: 667 512 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 512 191
SfoI GGC'GCC
Cuts at: 450 1601 1849 450
Size: 1151 248 2770
Fragments arranged by size:
2770 1151 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 1324 2239 2731 2993 1324
Size: 915 492 262 2500
Fragments arranged by size:
2500 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StuI AGG'CCT
Cuts at: 1099 1099
Size: 4169
StyI C'CwwG_G
Cuts at: 185 371 1148 1303 1657 185
Size: 186 777 155 354 2697
Fragments arranged by size:
2697 777 354 186 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1004 1120 1752 1885 2344 2553 1004
Size: 116 632 133 459 209 2620
Fragments arranged by size:
2620 632 459 209 133 116
TatI w'GTAC_w
Cuts at: 719 3684 719
Size: 2965 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 314 346 446 470 478 502 995 1027 3686
Size: 32 100 24 8 24 493 32 2659
Cuts at: 3686 3718 314
Size: 32 765
Fragments arranged by size:
2659 765 493 100 32 32 32 24
24 8
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 378 1664 294
Size: 84 1286 2799
Fragments arranged by size:
2799 1286 84
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI