Restriction map for
pF1KA1170
(Circular) (Six-base) MAPSORT of: pF1KA1170.seq Check: 1642 from: 1 to: 5387
pF1KA1170 5387 bp
With 182 enzymes: SgfI *
February 20, 2009 16:03 ..
AccI GT'mk_AC
Cuts at: 2425 2425
Size: 5387
Acc65I G'GTAC_C
Cuts at: 1596 2403 1596
Size: 807 4580
AclI AA'CG_TT
Cuts at: 5227 5227
Size: 5387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1040 1957 3212 3644 3997 4659 502
Size: 538 917 1255 432 353 662 1230
Fragments arranged by size:
1255 1230 917 662 538 432 353
AflII C'TTAA_G
Cuts at: 1669 1669
Size: 5387
AflIII A'CryG_T
Cuts at: 2739 4539 4820 2739
Size: 1800 281 3306
Fragments arranged by size:
3306 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2077 2109 2077
Size: 32 5355
AlwNI CAG_nnn'CTG
Cuts at: 2692 4130 2692
Size: 1438 3949
ApaI G_GGCC'C
Cuts at: 142 1371 2199 142
Size: 1229 828 3330
Fragments arranged by size:
3330 1229 828
ApaLI G'TGCA_C
Cuts at: 4225 4225
Size: 5387
ApoI r'AATT_y
Cuts at: 334 2391 4740 4996 334
Size: 2057 2349 256 725
Fragments arranged by size:
2349 2057 725 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5387
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
AvaI C'yCGr_G
Cuts at: 491 788 2407 491
Size: 297 1619 3471
Fragments arranged by size:
3471 1619 297
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1448 1481 1448
Size: 33 5354
BamHI G'GATC_C
Cuts at: 2412 2412
Size: 5387
BanI G'GyrC_C
Cuts at: 189 1101 1299 1320 1596 2178 2270 2403 2817
Size: 912 198 21 276 582 92 133 414
Cuts at: 2817 3065 3100 189
Size: 248 35 2476
Fragments arranged by size:
2476 912 582 414 276 248 198 133
92 35 21
BanII G_rGCy'C
Cuts at: 142 241 704 1214 1371 2199 2401 3431 142
Size: 99 463 510 157 828 202 1030 2098
Fragments arranged by size:
2098 1030 828 510 463 202 157 99
BbeI G_GCGC'C
Cuts at: 193 2274 2821 3069 193
Size: 2081 547 248 2511
Fragments arranged by size:
2511 2081 547 248
BbsI GAAGACnn'nnnn_
Cuts at: 1028 1504 2360 1028
Size: 476 856 4055
Fragments arranged by size:
4055 856 476
BciVI GTATCCnnnnn_n'
Cuts at: 1039 3285 4341 5387 1039
Size: 2246 1056 1046 1039
Fragments arranged by size:
2246 1056 1046 1039
BclI T'GATC_A
Cuts at: 2907 2907
Size: 5387
BfrBI ATG'CAT
Cuts at: 825 825
Size: 5387
BglI GCCn_nnn'nGGC
Cuts at: 424 1133 424
Size: 709 4678
BglII A'GATC_T
Cuts at: 2902 4939 2902
Size: 2037 3350
BlpI GC'TnA_GC
Cuts at: 1334 2499 1334
Size: 1165 4222
Bme1580I G_kGCm'C
Cuts at: 142 663 1371 1573 2199 3012 3105 4229 142
Size: 521 708 202 626 813 93 1124 1300
Fragments arranged by size:
1300 1124 813 708 626 521 202 93
BmrI ACTGGGnnnn_n'
Cuts at: 2355 2599 2680 3015 5159 2355
Size: 244 81 335 2144 2583
Fragments arranged by size:
2583 2144 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3754 3754
Size: 5387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 421 634 772 1582 1753 2078 2100 280
Size: 141 213 138 810 171 325 22 3567
Fragments arranged by size:
3567 810 325 213 171 141 138 22
Bpu10I CC'TnA_GC
Cuts at: 703 703
Size: 5387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 2185 2563 3442 4156 4454 362
Size: 1823 378 879 714 298 1295
Fragments arranged by size:
1823 1295 879 714 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 787 5091 787
Size: 4304 1083
BsaAI yAC'GTr
Cuts at: 2740 2740
Size: 5387
BsaBI GATnn'nnATC
Cuts at: 6 1756 2901 4938 6
Size: 1750 1145 2037 455
Fragments arranged by size:
2037 1750 1145 455
BsaHI Gr'CG_yC
Cuts at: 190 1606 2271 2818 3066 3768 5264 190
Size: 1416 665 547 248 702 1496 313
Fragments arranged by size:
1496 1416 702 665 547 313 248
BsaWI w'CCGG_w
Cuts at: 2576 3097 3775 4186 4333 4607 2576
Size: 521 678 411 147 274 3356
Fragments arranged by size:
3356 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 253
Size: 30 5357
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 847 881 1141 1222 1237 1246 1570 1762 1840
Size: 34 260 81 15 9 324 192 78
Cuts at: 1840 847
Size: 4394
Fragments arranged by size:
4394 324 260 192 81 78 34 15
9
BseYI C'CCAG_C
Cuts at: 1782 2812 3570 4235 4643 1782
Size: 1030 758 665 408 2526
Fragments arranged by size:
2526 1030 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1558 403
Size: 1155 4232
BsiEI CG_ry'CG
Cuts at: 104 2929 2975 4205 104
Size: 2825 46 1230 1286
Fragments arranged by size:
2825 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 704 1400 2401 3179 3369 4229 704
Size: 696 1001 778 190 860 1862
Fragments arranged by size:
1862 1001 860 778 696 190
BsmI GAATG_Cn'
Cuts at: 1637 1637
Size: 5387
BsmBI CGTCTCn'nnnn_
Cuts at: 1597 1597
Size: 5387
Bsp1286I G_dGCh'C
Cuts at: 142 241 663 704 1214 1371 1400 1573 2199
Size: 99 422 41 510 157 29 173 626
Cuts at: 2199 2401 3012 3105 3179 3369 3431 4229 142
Size: 202 611 93 74 190 62 798 1300
Fragments arranged by size:
1300 798 626 611 510 422 202 190
173 157 99 93 74 62 41 29
BspHI T'CATG_A
Cuts at: 3819 3819
Size: 5387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1286 2437 2651 2953 3334 1286
Size: 1151 214 302 381 3339
Fragments arranged by size:
3339 1151 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1338 3679 3733 5217 5382 50
Size: 1288 2341 54 1484 165 55
Fragments arranged by size:
2341 1484 1288 165 55 54
BsrDI GCAATG_nn'
Cuts at: 162 3299 162
Size: 3137 2250
BsrFI r'CCGG_y
Cuts at: 449 3385 3566 449
Size: 2936 181 2270
Fragments arranged by size:
2936 2270 181
BsrGI T'GTAC_A
Cuts at: 1637 1637
Size: 5387
BssSI C'ACGA_G
Cuts at: 3658 4366 3658
Size: 708 4679
BstAPI GCAn_nnn'nTGC
Cuts at: 2764 2764
Size: 5387
BstBI TT'CG_AA
Cuts at: 3748 3748
Size: 5387
BstEII G'GTnAC_C
Cuts at: 864 864
Size: 5387
BstXI CCAn_nnnn'nTGG
Cuts at: 612 2160 2265 612
Size: 1548 105 3734
Fragments arranged by size:
3734 1548 105
BstYI r'GATC_y
Cuts at: 1 1191 2412 2879 2902 3237 3483 3789 3801
Size: 1190 1221 467 23 335 246 306 12
Cuts at: 3801 3887 4939 1
Size: 86 1052 449
Fragments arranged by size:
1221 1190 1052 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1745 2110 1745
Size: 365 5022
BtgI C'CryG_G
Cuts at: 107 860 1131 107
Size: 753 271 4363
Fragments arranged by size:
4363 753 271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1512 3337 3518 3681 1512
Size: 1825 181 163 3218
Fragments arranged by size:
3218 1825 181 163
BtsI GCAGTG_nn'
Cuts at: 663 885 1970 2630 663
Size: 222 1085 660 3420
Fragments arranged by size:
3420 1085 660 222
ClaI AT'CG_AT
Cuts at: 1790 1790
Size: 5387
DraI TTT'AAA
Cuts at: 2172 2386 2172
Size: 214 5173
DrdI GACnn_nn'nnGTC
Cuts at: 3093 4437 3093
Size: 1344 4043
EaeI y'GGCC_r
Cuts at: 2972 3146 3537 3564 4909 2972
Size: 174 391 27 1345 3450
Fragments arranged by size:
3450 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2972 2972
Size: 5387
EarI CTCTTCn'nnn_
Cuts at: 859 1036 1201 1223 1547 1583 3410 3620 859
Size: 177 165 22 324 36 1827 210 2626
Fragments arranged by size:
2626 1827 324 210 177 165 36 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3595 4337 4483 5197 3595
Size: 742 146 714 3785
Fragments arranged by size:
3785 742 714 146
EcoICRI GAG'CTC
Cuts at: 702 2399 702
Size: 1697 3690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 421 502 634 772 1040 1582 1753 1957
Size: 141 81 132 138 268 542 171 204
Cuts at: 1957 2078 2100 3212 3644 3997 4659 280
Size: 121 22 1112 432 353 662 1008
Fragments arranged by size:
1112 1008 662 542 432 353 268 204
171 141 138 132 121 81 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1444 1624 1744 1444
Size: 180 120 5087
Fragments arranged by size:
5087 180 120
EcoO109I rG'GnC_Cy
Cuts at: 114 138 596 912 1290 1356 1368 2196 2526
Size: 24 458 316 378 66 12 828 330
Cuts at: 2526 114
Size: 2975
Fragments arranged by size:
2975 828 458 378 330 316 66 24
12
EcoRI G'AATT_C
Cuts at: 334 2391 334
Size: 2057 3330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5387
FspI TGC'GCA
Cuts at: 3168 3168
Size: 5387
HaeII r_GCGC'y
Cuts at: 193 2274 2821 3069 4299 5070 193
Size: 2081 547 248 1230 771 510
Fragments arranged by size:
2081 1230 771 547 510 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3229 3259 3261 3291 3229
Size: 30 2 30 5325
Fragments arranged by size:
5325 30 30 2
HincII GTy'rAC
Cuts at: 2426 2426
Size: 5387
HindIII A'AGCT_T
Cuts at: 813 813
Size: 5387
KasI G'GCGC_C
Cuts at: 189 2270 2817 3065 189
Size: 2081 547 248 2511
Fragments arranged by size:
2511 2081 547 248
KpnI G_GTAC'C
Cuts at: 1600 2407 1600
Size: 807 4580
MluI A'CGCG_T
Cuts at: 4820 4820
Size: 5387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 386 1446 4146 4330 386
Size: 1060 2700 184 1443
Fragments arranged by size:
2700 1443 1060 184
MscI TGG'CCA
Cuts at: 3148 3148
Size: 5387
MslI CAynn'nnrTG
Cuts at: 1490 1792 3376 3503 1490
Size: 302 1584 127 3374
Fragments arranged by size:
3374 1584 302 127
MspA1I CmG'CkG
Cuts at: 222 676 1098 2498 2812 3172 3956 4201 222
Size: 454 422 1400 314 360 784 245 1408
Fragments arranged by size:
1408 1400 784 454 422 360 314 245
NaeI GCC'GGC
Cuts at: 3568 3568
Size: 5387
NarI GG'CG_CC
Cuts at: 190 2271 2818 3066 190
Size: 2081 547 248 2511
Fragments arranged by size:
2511 2081 547 248
NcoI C'CATG_G
Cuts at: 107 860 107
Size: 753 4634
NgoMIV G'CCGG_C
Cuts at: 3566 3566
Size: 5387
NsiI A_TGCA'T
Cuts at: 827 827
Size: 5387
NspI r_CATG'y
Cuts at: 292 1050 2440 2940 4543 292
Size: 758 1390 500 1603 1136
Fragments arranged by size:
1603 1390 1136 758 500
PasI CC'CwG_GG
Cuts at: 1364 1440 1364
Size: 76 5311
PciI A'CATG_T
Cuts at: 4539 4539
Size: 5387
PfoI T'CCnGG_A
Cuts at: 611 1994 2018 2146 611
Size: 1383 24 128 3852
Fragments arranged by size:
3852 1383 128 24
PmeI GTTT'AAAC
Cuts at: 2386 2386
Size: 5387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 5355
PpuMI rG'GwC_Cy
Cuts at: 114 596 912 1290 114
Size: 482 316 378 4211
Fragments arranged by size:
4211 482 378 316
PspOMI G'GGCC_C
Cuts at: 138 1367 2195 138
Size: 1229 828 3330
Fragments arranged by size:
3330 1229 828
PstI C_TGCA'G
Cuts at: 880 971 1689 2434 3119 880
Size: 91 718 745 685 3148
Fragments arranged by size:
3148 745 718 685 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5387
PvuII CAG'CTG
Cuts at: 676 1098 2812 3172 676
Size: 422 1714 360 2891
Fragments arranged by size:
2891 1714 422 360
RsrII CG'GwC_CG
Cuts at: 3582 3582
Size: 5387
SacI G_AGCT'C
Cuts at: 704 2401 704
Size: 1697 3690
SalI G'TCGA_C
Cuts at: 2424 2424
Size: 5387
SapI GCTCTTCn'nnn_
Cuts at: 1036 3410 3620 1036
Size: 2374 210 2803
Fragments arranged by size:
2803 2374 210
SbfI CC_TGCA'GG
Cuts at: 2434 2434
Size: 5387
ScaI AGT'ACT
Cuts at: 4904 4904
Size: 5387
SfcI C'TryA_G
Cuts at: 33 651 876 967 1685 2430 3115 4083 4274
Size: 618 225 91 718 745 685 968 191
Cuts at: 4274 33
Size: 1146
Fragments arranged by size:
1146 968 745 718 685 618 225 191
91
SfoI GGC'GCC
Cuts at: 191 2272 2819 3067 191
Size: 2081 547 248 2511
Fragments arranged by size:
2511 2081 547 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
SmaI CCC'GGG
Cuts at: 493 790 2409 493
Size: 297 1619 3471
Fragments arranged by size:
3471 1619 297
SmlI C'TyrA_G
Cuts at: 377 1669 2200 2542 3457 4171 4433 377
Size: 1292 531 342 915 714 262 1331
Fragments arranged by size:
1331 1292 915 714 531 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5387
SphI G_CATG'C
Cuts at: 292 1050 2440 2940 292
Size: 758 1390 500 2739
Fragments arranged by size:
2739 1390 758 500
SrfI GCCC'GGGC
Cuts at: 493 493
Size: 5387
SspI AAT'ATT
Cuts at: 744 744
Size: 5387
StuI AGG'CCT
Cuts at: 427 649 427
Size: 222 5165
StyI C'CwwG_G
Cuts at: 107 194 513 860 1259 1352 2521 2875 107
Size: 87 319 347 399 93 1169 354 2619
Fragments arranged by size:
2619 1169 399 354 347 319 93 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 764 2970 3103 3562 3771 764
Size: 2206 133 459 209 2380
Fragments arranged by size:
2380 2206 459 209 133
TatI w'GTAC_w
Cuts at: 981 1637 4902 981
Size: 656 3265 1466
Fragments arranged by size:
3265 1466 656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4904 4936 4904
Size: 32 5355
Tth111I GACn'n_nGTC
Cuts at: 3184 3184
Size: 5387
XbaI T'CTAG_A
Cuts at: 2418 2418
Size: 5387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 520 2882 520
Size: 2362 3025
XmaI C'CCGG_G
Cuts at: 491 788 2407 491
Size: 297 1619 3471
Fragments arranged by size:
3471 1619 297
XmnI GAAnn'nnTTC
Cuts at: 743 1423 1942 743
Size: 680 519 4188
Fragments arranged by size:
4188 680 519
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AscI
AvrII BbvCI BcgI BmgBI BmtI BsiWI BspEI BssHII
BstZ17I CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SwaI XhoI ZraI