Restriction map for
pF1KA1153
(Circular) (Six-base) MAPSORT of: pF1KA1153.seq Check: 4101 from: 1 to: 4313
pF1KA1153 4313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 327 1351 327
Size: 1024 3289
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1255 2138 2570 3317 3585 920
Size: 335 883 432 747 268 1648
Fragments arranged by size:
1648 883 747 432 335 268
AflIII A'CryG_T
Cuts at: 730 1665 2769 3746 730
Size: 935 1104 977 1297
Fragments arranged by size:
1297 1104 977 935
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1236 1236
Size: 4313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 800 832 800
Size: 32 4281
AlwNI CAG_nnn'CTG
Cuts at: 1618 3185 1618
Size: 1567 2746
ApaI G_GGCC'C
Cuts at: 136 136
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 526 874 1317 3666 3922 526
Size: 348 443 2349 256 917
Fragments arranged by size:
2349 917 443 348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1333 1333
Size: 4313
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 1329 1743 1991 2026 1329
Size: 414 248 35 3616
Fragments arranged by size:
3616 414 248 35
BanII G_rGCy'C
Cuts at: 118 136 1266 1327 2357 118
Size: 18 1130 61 1030 2074
Fragments arranged by size:
2074 1130 1030 61 18
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BbsI GAAGACnn'nnnn_
Cuts at: 892 1022 1061 892
Size: 130 39 4144
Fragments arranged by size:
4144 130 39
BciVI GTATCCnnnnn_n'
Cuts at: 2211 2972 4313 2211
Size: 761 1341 2211
Fragments arranged by size:
2211 1341 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 136 382 1938 2031 3087 136
Size: 246 1556 93 1056 1362
Fragments arranged by size:
1556 1362 1056 246 93
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1003 824
Size: 179 4134
Bpu10I CC'TnA_GC
Cuts at: 117 117
Size: 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 1067 1489 2368 2860 3158 413
Size: 654 422 879 492 298 1568
Fragments arranged by size:
1568 879 654 492 422 298
BsaI GGTCTCn'nnnn_
Cuts at: 149 4017 149
Size: 3868 445
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 1744 1992 2694 4190 1744
Size: 248 702 1496 1867
Fragments arranged by size:
1867 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1502 2023 2701 2975 3122 3533 1502
Size: 521 678 274 147 411 2282
Fragments arranged by size:
2282 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 800 830 800
Size: 30 4283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 821 1164 1238 821
Size: 343 74 3896
Fragments arranged by size:
3896 343 74
BseYI C'CCAG_C
Cuts at: 747 1738 2496 3073 3569 747
Size: 991 758 577 496 1491
Fragments arranged by size:
1491 991 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 755
Size: 4313
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3109 104
Size: 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1327 2105 2295 3087 1327
Size: 778 190 792 2553
Fragments arranged by size:
2553 792 778 190
BsmI GAATG_Cn'
Cuts at: 1284 1284
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 118 136 382 1266 1327 1938 2031 2105 2295
Size: 18 246 884 61 611 93 74 190
Cuts at: 2295 2357 3087 118
Size: 62 730 1344
Fragments arranged by size:
1344 884 730 611 246 190 93 74
62 61 18
BspEI T'CCGG_A
Cuts at: 3533 3533
Size: 4313
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1192 2605 2659 4143 4308 50
Size: 1142 1413 54 1484 165 55
Fragments arranged by size:
1484 1413 1142 165 55 54
BsrDI GCAATG_nn'
Cuts at: 761 2225 761
Size: 1464 2849
BsrFI r'CCGG_y
Cuts at: 2311 2492 2701 2311
Size: 181 209 3923
Fragments arranged by size:
3923 209 181
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 248 2674 248
Size: 2426 1887
BstXI CCAn_nnnn'nTGG
Cuts at: 292 292
Size: 4313
BstYI r'GATC_y
Cuts at: 1 1086 1338 1805 1828 2163 2409 3421 3507
Size: 1085 252 467 23 335 246 1012 86
Cuts at: 3507 3519 3865 1
Size: 12 346 449
Fragments arranged by size:
1085 1012 467 449 346 335 252 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 2444 2607 2263
Size: 181 163 3969
Fragments arranged by size:
3969 181 163
BtsI GCAGTG_nn'
Cuts at: 1556 1556
Size: 4313
DraI TTT'AAA
Cuts at: 210 1312 210
Size: 1102 3211
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 282 1898 2072 2463 2490 3835 282
Size: 1616 174 391 27 1345 760
Fragments arranged by size:
1616 1345 760 391 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 337 344 1154 2336 2546 337
Size: 7 810 1182 210 2104
Fragments arranged by size:
2104 1182 810 210 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 2831 2977 4123 2521
Size: 310 146 1146 2711
Fragments arranged by size:
2711 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 920 1003 1255 2138 2570 3317 3585 824
Size: 96 83 252 883 432 747 268 1552
Fragments arranged by size:
1552 883 747 432 268 252 96 83
EcoNI CCTnn'n_nnAGG
Cuts at: 806 806
Size: 4313
EcoO109I rG'GnC_Cy
Cuts at: 811 1032 1102 1452 811
Size: 221 70 350 3672
Fragments arranged by size:
3672 350 221 70
EcoRI G'AATT_C
Cuts at: 874 1317 874
Size: 443 3870
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 1747 1995 3017 3996 1747
Size: 248 1022 979 2064
Fragments arranged by size:
2064 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 397 429 2155 2185 2187 2217 397
Size: 32 1726 30 2 30 2493
Fragments arranged by size:
2493 1726 32 30 30 2
HincII GTy'rAC
Cuts at: 683 1352 683
Size: 669 3644
HindIII A'AGCT_T
Cuts at: 443 443
Size: 4313
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2984 3168 2984
Size: 184 4129
MscI TGG'CCA
Cuts at: 284 2074 284
Size: 1790 2523
MslI CAynn'nnrTG
Cuts at: 290 2302 2429 290
Size: 2012 127 2174
Fragments arranged by size:
2174 2012 127
MspA1I CmG'CkG
Cuts at: 228 1424 1738 2098 3111 3356 228
Size: 1196 314 360 1013 245 1185
Fragments arranged by size:
1196 1185 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NcoI C'CATG_G
Cuts at: 107 107
Size: 4313
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NspI r_CATG'y
Cuts at: 836 1260 1366 1866 2773 836
Size: 424 106 500 907 2376
Fragments arranged by size:
2376 907 500 424 106
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PfoI T'CCnGG_A
Cuts at: 150 150
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 800 832 3452 3484 800
Size: 32 2620 32 1629
Fragments arranged by size:
2620 1629 32 32
PpuMI rG'GwC_Cy
Cuts at: 811 811
Size: 4313
PspOMI G'GGCC_C
Cuts at: 132 132
Size: 4313
PstI C_TGCA'G
Cuts at: 363 1360 2045 363
Size: 997 685 2631
Fragments arranged by size:
2631 997 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1738 2098 1738
Size: 360 3953
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 344 1154 2336 2546 344
Size: 810 1182 210 2111
Fragments arranged by size:
2111 1182 810 210
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 328 359 1356 2041 3034 3225 33
Size: 295 31 997 685 993 191 1121
Fragments arranged by size:
1121 997 993 685 295 191 31
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 428 1082 1468 2383 2875 3137 428
Size: 654 386 915 492 262 1604
Fragments arranged by size:
1604 915 654 492 386 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StyI C'CwwG_G
Cuts at: 107 1447 1801 107
Size: 1340 354 2619
Fragments arranged by size:
2619 1340 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 739 1064 1208 1896 2029 2488 2697 739
Size: 325 144 688 133 459 209 2355
Fragments arranged by size:
2355 688 459 325 209 144 133
TatI w'GTAC_w
Cuts at: 216 502 3828 216
Size: 286 3326 701
Fragments arranged by size:
3326 701 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
XmnI GAAnn'nnTTC
Cuts at: 479 915 479
Size: 436 3877
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI