Restriction map for
pF1KA1147
(Circular) (Six-base) MAPSORT of: pF1KA1147.seq Check: 5599 from: 1 to: 4478
pF1KA1147 4478 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1154 1154
Size: 4478
AatII G_ACGT'C
Cuts at: 1106 1106
Size: 4478
AccI GT'mk_AC
Cuts at: 743 1106 1516 743
Size: 363 410 3705
Fragments arranged by size:
3705 410 363
Acc65I G'GTAC_C
Cuts at: 1494 1494
Size: 4478
AclI AA'CG_TT
Cuts at: 953 4318 953
Size: 3365 1113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2303 2735 3088 3750 1180
Size: 1123 432 353 662 1908
Fragments arranged by size:
1908 1123 662 432 353
AflIII A'CryG_T
Cuts at: 770 1830 3630 3911 770
Size: 1060 1800 281 1337
Fragments arranged by size:
1800 1337 1060 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 4478
AlwNI CAG_nnn'CTG
Cuts at: 1155 1218 1783 3221 1155
Size: 63 565 1438 2412
Fragments arranged by size:
2412 1438 565 63
ApaI G_GGCC'C
Cuts at: 157 494 157
Size: 337 4141
ApaLI G'TGCA_C
Cuts at: 3316 3316
Size: 4478
ApoI r'AATT_y
Cuts at: 859 1482 3831 4087 859
Size: 623 2349 256 1250
Fragments arranged by size:
2349 1250 623 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
AvaI C'yCGr_G
Cuts at: 262 298 372 1367 1498 262
Size: 36 74 995 131 3242
Fragments arranged by size:
3242 995 131 74 36
BamHI G'GATC_C
Cuts at: 1503 1503
Size: 4478
BanI G'GyrC_C
Cuts at: 129 223 302 635 976 1494 1908 2156 2191
Size: 94 79 333 341 518 414 248 35
Cuts at: 2191 129
Size: 2416
Fragments arranged by size:
2416 518 414 341 333 248 94 79
35
BanII G_rGCy'C
Cuts at: 157 263 494 1492 2522 157
Size: 106 231 998 1030 2113
Fragments arranged by size:
2113 1030 998 231 106
BbeI G_GCGC'C
Cuts at: 133 227 306 1912 2160 133
Size: 94 79 1606 248 2451
Fragments arranged by size:
2451 1606 248 94 79
BbsI GAAGACnn'nnnn_
Cuts at: 1135 1250 1135
Size: 115 4363
BciVI GTATCCnnnnn_n'
Cuts at: 2376 3432 4478 2376
Size: 1056 1046 2376
Fragments arranged by size:
2376 1056 1046
BclI T'GATC_A
Cuts at: 1998 1998
Size: 4478
BglI GCCn_nnn'nGGC
Cuts at: 212 232 252 212
Size: 20 20 4438
Fragments arranged by size:
4438 20 20
BglII A'GATC_T
Cuts at: 1993 4030 1993
Size: 2037 2441
BlpI GC'TnA_GC
Cuts at: 1590 1590
Size: 4478
Bme1580I G_kGCm'C
Cuts at: 157 494 979 2103 2196 3320 157
Size: 337 485 1124 93 1124 1315
Fragments arranged by size:
1315 1124 1124 485 337 93
BmgBI CAC'GTC
Cuts at: 321 321
Size: 4478
BmrI ACTGGGnnnn_n'
Cuts at: 1690 1771 2106 4250 1690
Size: 81 335 2144 1918
Fragments arranged by size:
2144 1918 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 4478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1054 1441 328
Size: 726 387 3365
Fragments arranged by size:
3365 726 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1654 2533 3247 3545 386
Size: 1268 879 714 298 1319
Fragments arranged by size:
1319 1268 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1388 4182 1388
Size: 2794 1684
BsaAI yAC'GTr
Cuts at: 1008 1110 1831 1008
Size: 102 721 3655
Fragments arranged by size:
3655 721 102
BsaBI GATnn'nnATC
Cuts at: 6 787 847 1992 4029 6
Size: 781 60 1145 2037 455
Fragments arranged by size:
2037 1145 781 455 60
BsaHI Gr'CG_yC
Cuts at: 130 224 303 1103 1909 2157 2859 4355 130
Size: 94 79 800 806 248 702 1496 253
Fragments arranged by size:
1496 806 800 702 253 248 94 79
BsaWI w'CCGG_w
Cuts at: 1301 1397 1667 2188 2866 3277 3424 3698 1301
Size: 96 270 521 678 411 147 274 2081
Fragments arranged by size:
2081 678 521 411 274 270 147 96
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 340 1096 280
Size: 60 756 3662
Fragments arranged by size:
3662 756 60
BseYI C'CCAG_C
Cuts at: 143 200 312 798 1903 2661 3326 3734 143
Size: 57 112 486 1105 758 665 408 887
Fragments arranged by size:
1105 887 758 665 486 408 112 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1044 1044
Size: 4478
BsiEI CG_ry'CG
Cuts at: 104 232 236 275 2020 2066 3296 104
Size: 128 4 39 1745 46 1230 1286
Fragments arranged by size:
1745 1286 1230 128 46 39 4
BsiHKAI G_wGCw'C
Cuts at: 704 1492 2270 2460 3320 704
Size: 788 778 190 860 1862
Fragments arranged by size:
1862 860 788 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 116 168 116
Size: 52 4426
Bsp1286I G_dGCh'C
Cuts at: 157 263 494 704 979 1492 2103 2196 2270
Size: 106 231 210 275 513 611 93 74
Cuts at: 2270 2460 2522 3320 157
Size: 190 62 798 1315
Fragments arranged by size:
1315 798 611 513 275 231 210 190
106 93 74 62
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1154 1424 1528 1742 2044 2425 1154
Size: 270 104 214 302 381 3207
Fragments arranged by size:
3207 381 302 270 214 104
BsrBI CCG'CTC
Cuts at: 50 371 2770 2824 4308 4473 50
Size: 321 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 321 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2390 2390
Size: 4478
BsrFI r'CCGG_y
Cuts at: 231 270 1193 2476 2657 231
Size: 39 923 1283 181 2052
Fragments arranged by size:
2052 1283 923 181 39
BssHII G'CGCG_C
Cuts at: 551 551
Size: 4478
BssSI C'ACGA_G
Cuts at: 2749 3457 2749
Size: 708 3770
BstAPI GCAn_nnn'nTGC
Cuts at: 1155 1363 1855 1155
Size: 208 492 3778
Fragments arranged by size:
3778 492 208
BstBI TT'CG_AA
Cuts at: 873 2839 873
Size: 1966 2512
BstEII G'GTnAC_C
Cuts at: 354 354
Size: 4478
BstYI r'GATC_y
Cuts at: 1 842 1503 1970 1993 2328 2574 2880 2892
Size: 841 661 467 23 335 246 306 12
Cuts at: 2892 2978 4030 1
Size: 86 1052 449
Fragments arranged by size:
1052 841 661 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 236 527 107
Size: 129 291 4058
Fragments arranged by size:
4058 291 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2428 2609 2772 2428
Size: 181 163 4134
Fragments arranged by size:
4134 181 163
BtsI GCAGTG_nn'
Cuts at: 592 958 1721 592
Size: 366 763 3349
Fragments arranged by size:
3349 763 366
DraI TTT'AAA
Cuts at: 1477 1477
Size: 4478
DrdI GACnn_nn'nnGTC
Cuts at: 2184 3528 2184
Size: 1344 3134
EaeI y'GGCC_r
Cuts at: 229 233 272 341 440 947 2063 2237 2628
Size: 4 39 69 99 507 1116 174 391
Cuts at: 2628 2655 4000 229
Size: 27 1345 707
Fragments arranged by size:
1345 1116 707 507 391 174 99 69
39 27 4
EagI C'GGCC_G
Cuts at: 229 233 272 2063 229
Size: 4 39 1791 2644
Fragments arranged by size:
2644 1791 39 4
EarI CTCTTCn'nnn_
Cuts at: 1256 1276 2501 2711 1256
Size: 20 1225 210 3023
Fragments arranged by size:
3023 1225 210 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2686 3428 3574 4288 2686
Size: 742 146 714 2876
Fragments arranged by size:
2876 742 714 146
EcoICRI GAG'CTC
Cuts at: 1490 1490
Size: 4478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1054 1180 1441 2303 2735 3088 3750 328
Size: 726 126 261 862 432 353 662 1056
Fragments arranged by size:
1056 862 726 662 432 353 261 126
EcoNI CCTnn'n_nnAGG
Cuts at: 172 1420 172
Size: 1248 3230
EcoO109I rG'GnC_Cy
Cuts at: 153 154 177 449 490 491 1266 1617 153
Size: 1 23 272 41 1 775 351 3014
Fragments arranged by size:
3014 775 351 272 41 23 1 1
EcoRI G'AATT_C
Cuts at: 1482 1482
Size: 4478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 510 2497 510
Size: 1987 2491
FseI GG_CCGG'CC
Cuts at: 235 235
Size: 4478
FspI TGC'GCA
Cuts at: 2259 2259
Size: 4478
HaeII r_GCGC'y
Cuts at: 133 227 306 1912 2160 3390 4161 133
Size: 94 79 1606 248 1230 771 450
Fragments arranged by size:
1606 1230 771 450 248 94 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 418 450 827 859 879 911 2320
Size: 32 31 32 377 32 20 32 1409
Cuts at: 2320 2350 2352 2382 355
Size: 30 2 30 2451
Fragments arranged by size:
2451 1409 377 32 32 32 32 31
30 30 20 2
HincII GTy'rAC
Cuts at: 1517 1517
Size: 4478
KasI G'GCGC_C
Cuts at: 129 223 302 1908 2156 129
Size: 94 79 1606 248 2451
Fragments arranged by size:
2451 1606 248 94 79
KpnI G_GTAC'C
Cuts at: 1498 1498
Size: 4478
MluI A'CGCG_T
Cuts at: 3911 3911
Size: 4478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 1301 3237 3421 623
Size: 678 1936 184 1680
Fragments arranged by size:
1936 1680 678 184
MscI TGG'CCA
Cuts at: 442 949 2239 442
Size: 507 1290 2681
Fragments arranged by size:
2681 1290 507
MslI CAynn'nnrTG
Cuts at: 526 2467 2594 526
Size: 1941 127 2410
Fragments arranged by size:
2410 1941 127
MspA1I CmG'CkG
Cuts at: 135 238 802 1155 1589 1903 2263 3047 3292
Size: 103 564 353 434 314 360 784 245
Cuts at: 3292 135
Size: 1321
Fragments arranged by size:
1321 784 564 434 360 353 314 245
103
NaeI GCC'GGC
Cuts at: 233 272 1195 2659 233
Size: 39 923 1464 2052
Fragments arranged by size:
2052 1464 923 39
NarI GG'CG_CC
Cuts at: 130 224 303 1909 2157 130
Size: 94 79 1606 248 2451
Fragments arranged by size:
2451 1606 248 94 79
NcoI C'CATG_G
Cuts at: 107 107
Size: 4478
NgoMIV G'CCGG_C
Cuts at: 231 270 1193 2657 231
Size: 39 923 1464 2052
Fragments arranged by size:
2052 1464 923 39
NspI r_CATG'y
Cuts at: 525 612 774 813 1366 1531 2031 3634 525
Size: 87 162 39 553 165 500 1603 1369
Fragments arranged by size:
1603 1369 553 500 165 162 87 39
PciI A'CATG_T
Cuts at: 770 3630 770
Size: 2860 1618
PflMI CCAn_nnn'nTGG
Cuts at: 339 339
Size: 4478
PfoI T'CCnGG_A
Cuts at: 1409 1409
Size: 4478
PmeI GTTT'AAAC
Cuts at: 1477 1477
Size: 4478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 830 862 2924 2956 830
Size: 32 2062 32 2352
Fragments arranged by size:
2352 2062 32 32
PpuMI rG'GwC_Cy
Cuts at: 449 1266 449
Size: 817 3661
PsiI TTA'TAA
Cuts at: 823 823
Size: 4478
PspOMI G'GGCC_C
Cuts at: 153 490 153
Size: 337 4141
PstI C_TGCA'G
Cuts at: 296 1151 1525 2210 296
Size: 855 374 685 2564
Fragments arranged by size:
2564 855 685 374
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4478
PvuII CAG'CTG
Cuts at: 802 1903 2263 802
Size: 1101 360 3017
Fragments arranged by size:
3017 1101 360
RsrII CG'GwC_CG
Cuts at: 2673 2673
Size: 4478
SacI G_AGCT'C
Cuts at: 1492 1492
Size: 4478
SacII CC_GC'GG
Cuts at: 239 239
Size: 4478
SalI G'TCGA_C
Cuts at: 1515 1515
Size: 4478
SanDI GG'GwC_CC
Cuts at: 449 449
Size: 4478
SapI GCTCTTCn'nnn_
Cuts at: 2501 2711 2501
Size: 210 4268
SbfI CC_TGCA'GG
Cuts at: 1525 1525
Size: 4478
ScaI AGT'ACT
Cuts at: 3995 3995
Size: 4478
SexAI A'CCwGG_T
Cuts at: 332 626 332
Size: 294 4184
SfcI C'TryA_G
Cuts at: 33 292 921 1147 1521 2206 3174 3365 33
Size: 259 629 226 374 685 968 191 1146
Fragments arranged by size:
1146 968 685 629 374 259 226 191
SfoI GGC'GCC
Cuts at: 131 225 304 1910 2158 131
Size: 94 79 1606 248 2451
Fragments arranged by size:
2451 1606 248 94 79
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
SmaI CCC'GGG
Cuts at: 300 1369 1500 300
Size: 1069 131 3278
Fragments arranged by size:
3278 1069 131
SmlI C'TyrA_G
Cuts at: 401 1633 2548 3262 3524 401
Size: 1232 915 714 262 1355
Fragments arranged by size:
1355 1232 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4478
SphI G_CATG'C
Cuts at: 1366 1531 2031 1366
Size: 165 500 3813
Fragments arranged by size:
3813 500 165
SrfI GCCC'GGGC
Cuts at: 300 300
Size: 4478
StuI AGG'CCT
Cuts at: 1426 1426
Size: 4478
StyI C'CwwG_G
Cuts at: 107 1389 1612 1966 107
Size: 1282 223 354 2619
Fragments arranged by size:
2619 1282 354 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 812 2061 2194 2653 2862 812
Size: 1249 133 459 209 2428
Fragments arranged by size:
2428 1249 459 209 133
TatI w'GTAC_w
Cuts at: 929 1223 3993 929
Size: 294 2770 1414
Fragments arranged by size:
2770 1414 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3995 4027 3995
Size: 32 4446
Tth111I GACn'n_nGTC
Cuts at: 2275 2275
Size: 4478
XbaI T'CTAG_A
Cuts at: 1509 1509
Size: 4478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 525 1973 525
Size: 1448 3030
XmaI C'CCGG_G
Cuts at: 298 1367 1498 298
Size: 1069 131 3278
Fragments arranged by size:
3278 1069 131
XmnI GAAnn'nnTTC
Cuts at: 1075 1075
Size: 4478
ZraI GAC'GTC
Cuts at: 1104 1104
Size: 4478
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BspEI BsrGI BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PspXI
PsrI SfiI SgrAI SnaBI SspI SwaI XhoI