Restriction map for
pF1KA1145
(Circular) (Six-base) MAPSORT of: pF1KA1145.seq Check: 3907 from: 1 to: 4451
pF1KA1145 4451 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 514 967 1096 2276 2708 3061 3723 247
Size: 267 453 129 1180 432 353 662 975
Fragments arranged by size:
1180 975 662 453 432 353 267 129
AfeI AGC'GCT
Cuts at: 741 1129 741
Size: 388 4063
AflII C'TTAA_G
Cuts at: 117 117
Size: 4451
AflIII A'CryG_T
Cuts at: 1803 3603 3884 1803
Size: 1800 281 2370
Fragments arranged by size:
2370 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 990 1086 1756 3194 990
Size: 96 670 1438 2247
Fragments arranged by size:
2247 1438 670 96
ApaLI G'TGCA_C
Cuts at: 3289 3289
Size: 4451
ApoI r'AATT_y
Cuts at: 236 687 1455 3804 4060 236
Size: 451 768 2349 256 627
Fragments arranged by size:
2349 768 627 451 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 1471 1471
Size: 4451
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 1357 1467 1881 2129 2164 1357
Size: 110 414 248 35 3644
Fragments arranged by size:
3644 414 248 110 35
BanII G_rGCy'C
Cuts at: 469 1190 1465 2495 469
Size: 721 275 1030 2425
Fragments arranged by size:
2425 1030 721 275
BbeI G_GCGC'C
Cuts at: 1885 2133 1885
Size: 248 4203
BbvCI CC'TCA_GC
Cuts at: 929 929
Size: 4451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 406 440 655 689 406
Size: 34 215 34 4168
Fragments arranged by size:
4168 215 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 903 2349 3405 4451 903
Size: 1446 1056 1046 903
Fragments arranged by size:
1446 1056 1046 903
BclI T'GATC_A
Cuts at: 1971 1971
Size: 4451
BglI GCCn_nnn'nGGC
Cuts at: 1117 1117
Size: 4451
BglII A'GATC_T
Cuts at: 1966 4003 1966
Size: 2037 2414
BlpI GC'TnA_GC
Cuts at: 920 1563 920
Size: 643 3808
Bme1580I G_kGCm'C
Cuts at: 2076 2169 3293 2076
Size: 93 1124 3234
Fragments arranged by size:
3234 1124 93
BmgBI CAC'GTC
Cuts at: 797 797
Size: 4451
BmrI ACTGGGnnnn_n'
Cuts at: 1410 1663 1744 2079 4223 1410
Size: 253 81 335 2144 1638
Fragments arranged by size:
2144 1638 335 253 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1042 1204 907
Size: 135 162 4154
Fragments arranged by size:
4154 162 135
Bpu10I CC'TnA_GC
Cuts at: 929 929
Size: 4451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 2506 3220 3518 1627
Size: 879 714 298 2560
Fragments arranged by size:
2560 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 591 4155 591
Size: 3564 887
BsaAI yAC'GTr
Cuts at: 528 1804 528
Size: 1276 3175
BsaBI GATnn'nnATC
Cuts at: 6 503 1965 4002 6
Size: 497 1462 2037 455
Fragments arranged by size:
2037 1462 497 455
BsaHI Gr'CG_yC
Cuts at: 1882 2130 2832 4328 1882
Size: 248 702 1496 2005
Fragments arranged by size:
2005 1496 702 248
BsaWI w'CCGG_w
Cuts at: 644 1640 2161 2839 3250 3397 3671 644
Size: 996 521 678 411 147 274 1424
Fragments arranged by size:
1424 996 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 712 1114 712
Size: 402 4049
BseYI C'CCAG_C
Cuts at: 395 1876 2634 3299 3707 395
Size: 1481 758 665 408 1139
Fragments arranged by size:
1481 1139 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 973
Size: 4451
BsiEI CG_ry'CG
Cuts at: 104 809 1993 2039 3269 104
Size: 705 1184 46 1230 1286
Fragments arranged by size:
1286 1230 1184 705 46
BsiHKAI G_wGCw'C
Cuts at: 469 1190 1465 2243 2433 3293 469
Size: 721 275 778 190 860 1627
Fragments arranged by size:
1627 860 778 721 275 190
BsmI GAATG_Cn'
Cuts at: 1236 1236
Size: 4451
BsmBI CGTCTCn'nnnn_
Cuts at: 1326 1326
Size: 4451
Bsp1286I G_dGCh'C
Cuts at: 469 1190 1465 2076 2169 2243 2433 2495 3293
Size: 721 275 611 93 74 190 62 798
Cuts at: 3293 469
Size: 1627
Fragments arranged by size:
1627 798 721 611 275 190 93 74
62
BspEI T'CCGG_A
Cuts at: 644 644
Size: 4451
BspHI T'CATG_A
Cuts at: 1277 2883 1277
Size: 1606 2845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1058 1501 1715 2017 2398 1058
Size: 443 214 302 381 3111
Fragments arranged by size:
3111 443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2743 2797 4281 4446 50
Size: 2693 54 1484 165 55
Fragments arranged by size:
2693 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 549 671 2363 549
Size: 122 1692 2637
Fragments arranged by size:
2637 1692 122
BsrFI r'CCGG_y
Cuts at: 2449 2630 2449
Size: 181 4270
BssSI C'ACGA_G
Cuts at: 2722 3430 2722
Size: 708 3743
BstAPI GCAn_nnn'nTGC
Cuts at: 1212 1828 1212
Size: 616 3835
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstXI CCAn_nnnn'nTGG
Cuts at: 832 855 1354 832
Size: 23 499 3929
Fragments arranged by size:
3929 499 23
BstYI r'GATC_y
Cuts at: 1 1476 1943 1966 2301 2547 2853 2865 2951
Size: 1475 467 23 335 246 306 12 86
Cuts at: 2951 4003 1
Size: 1052 449
Fragments arranged by size:
1475 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 260 296 375 490 1293 2401 2582 2745 260
Size: 36 79 115 803 1108 181 163 1966
Fragments arranged by size:
1966 1108 803 181 163 115 79 36
BtsI GCAGTG_nn'
Cuts at: 212 777 1694 212
Size: 565 917 2969
Fragments arranged by size:
2969 917 565
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3501 2157
Size: 1344 3107
EaeI y'GGCC_r
Cuts at: 806 1088 2036 2210 2601 2628 3973 806
Size: 282 948 174 391 27 1345 1284
Fragments arranged by size:
1345 1284 948 391 282 174 27
EagI C'GGCC_G
Cuts at: 806 2036 806
Size: 1230 3221
EarI CTCTTCn'nnn_
Cuts at: 1330 1382 2474 2684 1330
Size: 52 1092 210 3097
Fragments arranged by size:
3097 1092 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2659 3401 3547 4261 2659
Size: 742 146 714 2849
Fragments arranged by size:
2849 742 714 146
EcoICRI GAG'CTC
Cuts at: 467 1188 1463 467
Size: 721 275 3455
Fragments arranged by size:
3455 721 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 514 907 967 1042 1096 1204 2276 2708
Size: 267 393 60 75 54 108 1072 432
Cuts at: 2708 3061 3723 247
Size: 353 662 975
Fragments arranged by size:
1072 975 662 432 393 353 267 108
75 60 54
EcoNI CCTnn'n_nnAGG
Cuts at: 994 1078 994
Size: 84 4367
EcoO109I rG'GnC_Cy
Cuts at: 725 1590 725
Size: 865 3586
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 2470 372
Size: 2098 2353
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 665 743 1131 1885 2133 3363 4134 665
Size: 78 388 754 248 1230 771 982
Fragments arranged by size:
1230 982 771 754 388 248 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 1323 1355 2293 2323 2325 2355 259
Size: 32 1032 32 938 30 2 30 2355
Fragments arranged by size:
2355 1032 938 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1490 1490
Size: 4451
KasI G'GCGC_C
Cuts at: 1881 2129 1881
Size: 248 4203
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3210 3394 3210
Size: 184 4267
MscI TGG'CCA
Cuts at: 1090 2212 1090
Size: 1122 3329
MslI CAynn'nnrTG
Cuts at: 1058 1261 1291 2440 2567 1058
Size: 203 30 1149 127 2942
Fragments arranged by size:
2942 1149 203 127 30
MspA1I CmG'CkG
Cuts at: 399 924 1032 1562 1876 2236 3020 3265 399
Size: 525 108 530 314 360 784 245 1585
Fragments arranged by size:
1585 784 530 525 360 314 245 108
NaeI GCC'GGC
Cuts at: 2632 2632
Size: 4451
NarI GG'CG_CC
Cuts at: 1882 2130 1882
Size: 248 4203
NgoMIV G'CCGG_C
Cuts at: 2630 2630
Size: 4451
NruI TCG'CGA
Cuts at: 1312 1312
Size: 4451
NspI r_CATG'y
Cuts at: 579 1504 2004 3607 579
Size: 925 500 1603 1423
Fragments arranged by size:
1603 1423 925 500
PciI A'CATG_T
Cuts at: 3603 3603
Size: 4451
PflMI CCAn_nnn'nTGG
Cuts at: 555 555
Size: 4451
PfoI T'CCnGG_A
Cuts at: 176 188 884 1142 176
Size: 12 696 258 3485
Fragments arranged by size:
3485 696 258 12
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PmlI CAC'GTG
Cuts at: 528 528
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 4419
PstI C_TGCA'G
Cuts at: 219 404 1217 1498 2183 219
Size: 185 813 281 685 2487
Fragments arranged by size:
2487 813 685 281 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 399 924 1032 1876 2236 399
Size: 525 108 844 360 2614
Fragments arranged by size:
2614 844 525 360 108
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 469 1190 1465 469
Size: 721 275 3455
Fragments arranged by size:
3455 721 275
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 2474 2684 2474
Size: 210 4241
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SfcI C'TryA_G
Cuts at: 33 215 400 1213 1494 2179 3147 3338 33
Size: 182 185 813 281 685 968 191 1146
Fragments arranged by size:
1146 968 813 685 281 191 185 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 1117 1117
Size: 4451
SfoI GGC'GCC
Cuts at: 1883 2131 1883
Size: 248 4203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SmaI CCC'GGG
Cuts at: 1473 1473
Size: 4451
SmlI C'TyrA_G
Cuts at: 117 1606 2521 3235 3497 117
Size: 1489 915 714 262 1071
Fragments arranged by size:
1489 1071 915 714 262
SpeI A'CTAG_T
Cuts at: 67 309 67
Size: 242 4209
SphI G_CATG'C
Cuts at: 579 1504 2004 579
Size: 925 500 3026
Fragments arranged by size:
3026 925 500
StuI AGG'CCT
Cuts at: 1120 1120
Size: 4451
StyI C'CwwG_G
Cuts at: 1151 1585 1939 1151
Size: 434 354 3663
Fragments arranged by size:
3663 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1341 2034 2167 2626 2835 1341
Size: 693 133 459 209 2957
Fragments arranged by size:
2957 693 459 209 133
TatI w'GTAC_w
Cuts at: 3966 3966
Size: 4451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4419
Tth111I GACn'n_nGTC
Cuts at: 2248 2248
Size: 4451
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1946 1946
Size: 4451
XmaI C'CCGG_G
Cuts at: 1471 1471
Size: 4451
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BmtI BsaXI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PasI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI