Restriction map for
pF1KA1144
(Circular) (Six-base) MAPSORT of: pF1KA1144.seq Check: 5012 from: 1 to: 4544
pF1KA1144 4544 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 302 302
Size: 4544
AccI GT'mk_AC
Cuts at: 1582 1582
Size: 4544
Acc65I G'GTAC_C
Cuts at: 1560 1560
Size: 4544
AclI AA'CG_TT
Cuts at: 4384 4384
Size: 4544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 657 2369 2801 3548 3816 393
Size: 264 1712 432 747 268 1121
Fragments arranged by size:
1712 1121 747 432 268 264
AflIII A'CryG_T
Cuts at: 131 1536 1896 3000 3977 131
Size: 1405 360 1104 977 698
Fragments arranged by size:
1405 1104 977 698 360
AgeI A'CCGG_T
Cuts at: 2932 2932
Size: 4544
AhdI GACnn_n'nnGTC
Cuts at: 903 903
Size: 4544
AleI CACnn'nnGTG
Cuts at: 931 931
Size: 4544
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 769 801 769
Size: 32 4512
AlwNI CAG_nnn'CTG
Cuts at: 123 996 1849 3416 123
Size: 873 853 1567 1251
Fragments arranged by size:
1567 1251 873 853
ApaLI G'TGCA_C
Cuts at: 3314 3314
Size: 4544
ApoI r'AATT_y
Cuts at: 1548 3897 4153 1548
Size: 2349 256 1939
Fragments arranged by size:
2349 1939 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4544
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4544
AvaI C'yCGr_G
Cuts at: 223 496 1564 223
Size: 273 1068 3203
Fragments arranged by size:
3203 1068 273
AvrII C'CTAG_G
Cuts at: 781 781
Size: 4544
BamHI G'GATC_C
Cuts at: 1569 1569
Size: 4544
BanI G'GyrC_C
Cuts at: 1361 1560 1974 2222 2257 1361
Size: 199 414 248 35 3648
Fragments arranged by size:
3648 414 248 199 35
BanII G_rGCy'C
Cuts at: 281 338 497 1504 1558 2588 281
Size: 57 159 1007 54 1030 2237
Fragments arranged by size:
2237 1030 1007 159 57 54
BbeI G_GCGC'C
Cuts at: 1365 1978 2226 1365
Size: 613 248 3683
Fragments arranged by size:
3683 613 248
BbsI GAAGACnn'nnnn_
Cuts at: 393 536 663 393
Size: 143 127 4274
Fragments arranged by size:
4274 143 127
BciVI GTATCCnnnnn_n'
Cuts at: 2442 3203 4544 2442
Size: 761 1341 2442
Fragments arranged by size:
2442 1341 761
BclI T'GATC_A
Cuts at: 2064 2064
Size: 4544
BglII A'GATC_T
Cuts at: 2059 4096 2059
Size: 2037 2507
BlpI GC'TnA_GC
Cuts at: 389 661 1656 389
Size: 272 995 3277
Fragments arranged by size:
3277 995 272
Bme1580I G_kGCm'C
Cuts at: 2169 2262 3318 2169
Size: 93 1056 3395
Fragments arranged by size:
3395 1056 93
BmgBI CAC'GTC
Cuts at: 132 381 542 132
Size: 249 161 4134
Fragments arranged by size:
4134 249 161
BmrI ACTGGGnnnn_n'
Cuts at: 438 1756 1837 2172 4316 438
Size: 1318 81 335 2144 666
Fragments arranged by size:
2144 1318 666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 4544
Bpu10I CC'TnA_GC
Cuts at: 332 332
Size: 4544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1396 1720 2599 3091 3389 850
Size: 546 324 879 492 298 2005
Fragments arranged by size:
2005 879 546 492 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 4248 4248
Size: 4544
BsaAI yAC'GTr
Cuts at: 348 1537 1897 348
Size: 1189 360 2995
Fragments arranged by size:
2995 1189 360
BsaBI GATnn'nnATC
Cuts at: 6 166 1009 1129 2058 4095 6
Size: 160 843 120 929 2037 455
Fragments arranged by size:
2037 929 843 455 160 120
BsaHI Gr'CG_yC
Cuts at: 299 578 1362 1975 2223 2925 4421 299
Size: 279 784 613 248 702 1496 422
Fragments arranged by size:
1496 784 702 613 422 279 248
BsaWI w'CCGG_w
Cuts at: 1733 2254 2932 3206 3353 3764 1733
Size: 521 678 274 147 411 2513
Fragments arranged by size:
2513 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 409 439 565 595 409
Size: 30 126 30 4358
Fragments arranged by size:
4358 126 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 1180 204
Size: 976 3568
BseYI C'CCAG_C
Cuts at: 1969 2727 3304 3800 1969
Size: 758 577 496 2713
Fragments arranged by size:
2713 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 324 2086 2132 3340 104
Size: 220 1762 46 1208 1308
Fragments arranged by size:
1762 1308 1208 220 46
BsiHKAI G_wGCw'C
Cuts at: 281 338 538 803 958 1504 1558 2336 2526
Size: 57 200 265 155 546 54 778 190
Cuts at: 2526 3318 281
Size: 792 1507
Fragments arranged by size:
1507 792 778 546 265 200 190 155
57 54
BsmI GAATG_Cn'
Cuts at: 885 885
Size: 4544
BsmBI CGTCTCn'nnnn_
Cuts at: 220 220
Size: 4544
Bsp1286I G_dGCh'C
Cuts at: 281 338 497 538 803 958 1504 1558 2169
Size: 57 159 41 265 155 546 54 611
Cuts at: 2169 2262 2336 2526 2588 3318 281
Size: 93 74 190 62 730 1507
Fragments arranged by size:
1507 730 611 546 265 190 159 155
93 74 62 57 54 41
BspEI T'CCGG_A
Cuts at: 3764 3764
Size: 4544
BspHI T'CATG_A
Cuts at: 3720 3720
Size: 4544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1594 1808 2110 2491 1594
Size: 214 302 381 3647
Fragments arranged by size:
3647 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1055 2836 2890 4374 4539 50
Size: 1005 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 1005 165 55 54
BsrDI GCAATG_nn'
Cuts at: 809 2456 809
Size: 1647 2897
BsrFI r'CCGG_y
Cuts at: 112 367 1358 2542 2723 2932 112
Size: 255 991 1184 181 209 1724
Fragments arranged by size:
1724 1184 991 255 209 181
BssSI C'ACGA_G
Cuts at: 1306 2815 3173 1306
Size: 1509 358 2677
Fragments arranged by size:
2677 1509 358
BstAPI GCAn_nnn'nTGC
Cuts at: 210 1921 210
Size: 1711 2833
BstBI TT'CG_AA
Cuts at: 788 2905 788
Size: 2117 2427
BstXI CCAn_nnnn'nTGG
Cuts at: 693 693
Size: 4544
BstYI r'GATC_y
Cuts at: 1 161 557 1010 1569 2036 2059 2394 2640
Size: 160 396 453 559 467 23 335 246
Cuts at: 2640 3652 3738 3750 4096 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 559 467 453 449 396 346 335
246 160 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 997 997
Size: 4544
BtgI C'CryG_G
Cuts at: 477 1118 1139 1259 477
Size: 641 21 120 3762
Fragments arranged by size:
3762 641 120 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 299 2494 2675 2838 299
Size: 2195 181 163 2005
Fragments arranged by size:
2195 2005 181 163
BtsI GCAGTG_nn'
Cuts at: 1787 1787
Size: 4544
DraI TTT'AAA
Cuts at: 1523 1543 1523
Size: 20 4524
DraIII CAC_nnn'GTG
Cuts at: 719 1510 719
Size: 791 3753
DrdI GACnn_nn'nnGTC
Cuts at: 2250 3108 2250
Size: 858 3686
EaeI y'GGCC_r
Cuts at: 114 480 836 1031 1181 2129 2303 2694 2721
Size: 366 356 195 150 948 174 391 27
Cuts at: 2721 4066 114
Size: 1345 592
Fragments arranged by size:
1345 948 592 391 366 356 195 174
150 27
EagI C'GGCC_G
Cuts at: 2129 2129
Size: 4544
EarI CTCTTCn'nnn_
Cuts at: 343 2567 2777 343
Size: 2224 210 2110
Fragments arranged by size:
2224 2110 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2752 3062 3208 4354 2752
Size: 310 146 1146 2942
Fragments arranged by size:
2942 1146 310 146
EcoICRI GAG'CTC
Cuts at: 279 336 1502 1556 279
Size: 57 1166 54 3267
Fragments arranged by size:
3267 1166 57 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 393 657 2369 2801 3548 3816 295
Size: 98 264 1712 432 747 268 1023
Fragments arranged by size:
1712 1023 747 432 268 264 98
EcoO109I rG'GnC_Cy
Cuts at: 777 993 1176 1422 1683 777
Size: 216 183 246 261 3638
Fragments arranged by size:
3638 261 246 216 183
EcoRI G'AATT_C
Cuts at: 1548 1548
Size: 4544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4544
FspI TGC'GCA
Cuts at: 1390 2325 1390
Size: 935 3609
HaeII r_GCGC'y
Cuts at: 637 1365 1978 2226 3248 4227 637
Size: 728 613 248 1022 979 954
Fragments arranged by size:
1022 979 954 728 613 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 889 921 2386 2416 2418 2448 889
Size: 32 1465 30 2 30 2985
Fragments arranged by size:
2985 1465 32 30 30 2
HincII GTy'rAC
Cuts at: 1583 1583
Size: 4544
HindIII A'AGCT_T
Cuts at: 373 373
Size: 4544
KasI G'GCGC_C
Cuts at: 1361 1974 2222 1361
Size: 613 248 3683
Fragments arranged by size:
3683 613 248
KpnI G_GTAC'C
Cuts at: 1564 1564
Size: 4544
MluI A'CGCG_T
Cuts at: 3977 3977
Size: 4544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 116 3215 3399 116
Size: 3099 184 1261
Fragments arranged by size:
3099 1261 184
MscI TGG'CCA
Cuts at: 838 1033 1183 2305 838
Size: 195 150 1122 3077
Fragments arranged by size:
3077 1122 195 150
MslI CAynn'nnrTG
Cuts at: 172 691 715 931 1306 2533 2660 172
Size: 519 24 216 375 1227 127 2056
Fragments arranged by size:
2056 1227 519 375 216 127 24
MspA1I CmG'CkG
Cuts at: 306 624 1394 1655 1969 2329 3342 3587 306
Size: 318 770 261 314 360 1013 245 1263
Fragments arranged by size:
1263 1013 770 360 318 314 261 245
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 4544
NarI GG'CG_CC
Cuts at: 1362 1975 2223 1362
Size: 613 248 3683
Fragments arranged by size:
3683 613 248
NcoI C'CATG_G
Cuts at: 477 477
Size: 4544
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 4544
NspI r_CATG'y
Cuts at: 1597 2097 3004 1597
Size: 500 907 3137
Fragments arranged by size:
3137 907 500
PasI CC'CwG_GG
Cuts at: 1059 1252 1059
Size: 193 4351
PciI A'CATG_T
Cuts at: 3000 3000
Size: 4544
PmeI GTTT'AAAC
Cuts at: 1543 1543
Size: 4544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4512
PpuMI rG'GwC_Cy
Cuts at: 777 993 1422 777
Size: 216 429 3899
Fragments arranged by size:
3899 429 216
PstI C_TGCA'G
Cuts at: 466 1591 2276 466
Size: 1125 685 2734
Fragments arranged by size:
2734 1125 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4544
PvuII CAG'CTG
Cuts at: 624 1394 1969 2329 624
Size: 770 575 360 2839
Fragments arranged by size:
2839 770 575 360
RsrII CG'GwC_CG
Cuts at: 2739 2739
Size: 4544
SacI G_AGCT'C
Cuts at: 281 338 1504 1558 281
Size: 57 1166 54 3267
Fragments arranged by size:
3267 1166 57 54
SalI G'TCGA_C
Cuts at: 1581 1581
Size: 4544
SapI GCTCTTCn'nnn_
Cuts at: 343 2567 2777 343
Size: 2224 210 2110
Fragments arranged by size:
2224 2110 210
SbfI CC_TGCA'GG
Cuts at: 1591 1591
Size: 4544
ScaI AGT'ACT
Cuts at: 427 4061 427
Size: 3634 910
SexAI A'CCwGG_T
Cuts at: 941 941
Size: 4544
SfcI C'TryA_G
Cuts at: 33 462 468 1587 2272 3265 3456 33
Size: 429 6 1119 685 993 191 1121
Fragments arranged by size:
1121 1119 993 685 429 191 6
SfoI GGC'GCC
Cuts at: 1363 1976 2224 1363
Size: 613 248 3683
Fragments arranged by size:
3683 613 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4544
SmaI CCC'GGG
Cuts at: 1566 1566
Size: 4544
SmlI C'TyrA_G
Cuts at: 865 1375 1699 2614 3106 3368 865
Size: 510 324 915 492 262 2041
Fragments arranged by size:
2041 915 510 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4544
SphI G_CATG'C
Cuts at: 1597 2097 1597
Size: 500 4044
StyI C'CwwG_G
Cuts at: 477 781 1678 2032 477
Size: 304 897 354 2989
Fragments arranged by size:
2989 897 354 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 2127 2260 2719 2928 1419
Size: 708 133 459 209 3035
Fragments arranged by size:
3035 708 459 209 133
TatI w'GTAC_w
Cuts at: 425 4059 425
Size: 3634 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4061 4093 4061
Size: 32 4512
Tth111I GACn'n_nGTC
Cuts at: 298 2341 298
Size: 2043 2501
XbaI T'CTAG_A
Cuts at: 1575 1575
Size: 4544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 766 846 1137 2039 766
Size: 80 291 902 3271
Fragments arranged by size:
3271 902 291 80
XmaI C'CCGG_G
Cuts at: 1564 1564
Size: 4544
ZraI GAC'GTC
Cuts at: 300 300
Size: 4544
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI ApaI AscI BaeI BbvCI
BcgI BfrBI BglI BmtI BsgI BsiWI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI