Restriction map for
pF1KA1141
(Circular) (Six-base) MAPSORT of: pF1KA1141.seq Check: 3618 from: 1 to: 5726
pF1KA1141 5726 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2764 2764
Size: 5726
Acc65I G'GTAC_C
Cuts at: 2742 2742
Size: 5726
AclI AA'CG_TT
Cuts at: 2236 2643 5566 2236
Size: 407 2923 2396
Fragments arranged by size:
2923 2396 407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 1326 1717 1999 2055 3551 3983 4730 4998
Size: 582 391 282 56 1496 432 747 268
Cuts at: 4998 744
Size: 1472
Fragments arranged by size:
1496 1472 747 582 432 391 282 268
56
AflIII A'CryG_T
Cuts at: 194 3078 4182 5159 194
Size: 2884 1104 977 761
Fragments arranged by size:
2884 1104 977 761
AgeI A'CCGG_T
Cuts at: 4114 4114
Size: 5726
AleI CACnn'nnGTG
Cuts at: 1709 2282 1709
Size: 573 5153
AlwNI CAG_nnn'CTG
Cuts at: 2604 3031 4598 2604
Size: 427 1567 3732
Fragments arranged by size:
3732 1567 427
ApaLI G'TGCA_C
Cuts at: 2081 4496 2081
Size: 2415 3311
ApoI r'AATT_y
Cuts at: 118 895 1460 2273 2357 2525 2730 5079 5335
Size: 777 565 813 84 168 205 2349 256
Cuts at: 5335 118
Size: 509
Fragments arranged by size:
2349 813 777 565 509 256 205 168
84
AseI AT'TA_AT
Cuts at: 20 20
Size: 5726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5726
AvaI C'yCGr_G
Cuts at: 175 2670 2746 175
Size: 2495 76 3155
Fragments arranged by size:
3155 2495 76
BamHI G'GATC_C
Cuts at: 2751 2751
Size: 5726
BanI G'GyrC_C
Cuts at: 1118 2663 2742 3156 3404 3439 1118
Size: 1545 79 414 248 35 3405
Fragments arranged by size:
3405 1545 414 248 79 35
BanII G_rGCy'C
Cuts at: 2251 2419 2740 3770 2251
Size: 168 321 1030 4207
Fragments arranged by size:
4207 1030 321 168
BbeI G_GCGC'C
Cuts at: 3160 3408 3160
Size: 248 5478
BbsI GAAGACnn'nnnn_
Cuts at: 493 2145 493
Size: 1652 4074
BbvCI CC'TCA_GC
Cuts at: 113 2677 113
Size: 2564 3162
BciVI GTATCCnnnnn_n'
Cuts at: 881 3624 4385 5726 881
Size: 2743 761 1341 881
Fragments arranged by size:
2743 1341 881 761
BclI T'GATC_A
Cuts at: 3246 3246
Size: 5726
BglII A'GATC_T
Cuts at: 288 1754 3241 5278 288
Size: 1466 1487 2037 736
Fragments arranged by size:
2037 1487 1466 736
BlpI GC'TnA_GC
Cuts at: 2336 2838 2336
Size: 502 5224
Bme1580I G_kGCm'C
Cuts at: 2085 2589 3351 3444 4500 2085
Size: 504 762 93 1056 3311
Fragments arranged by size:
3311 1056 762 504 93
BmrI ACTGGGnnnn_n'
Cuts at: 174 1004 2459 2938 3019 3354 5498 174
Size: 830 1455 479 81 335 2144 402
Fragments arranged by size:
2144 1455 830 479 402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 696 4093 696
Size: 3397 2329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 989 1322 2135 2387 313
Size: 676 333 813 252 3652
Fragments arranged by size:
3652 813 676 333 252
Bpu10I CC'TnA_GC
Cuts at: 113 2677 113
Size: 2564 3162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 1200 2902 3781 4273 4571 115
Size: 1085 1702 879 492 298 1270
Fragments arranged by size:
1702 1270 1085 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 337 5430 337
Size: 5093 633
BsaAI yAC'GTr
Cuts at: 3079 3079
Size: 5726
BsaBI GATnn'nnATC
Cuts at: 6 1604 3240 5277 6
Size: 1598 1636 2037 455
Fragments arranged by size:
2037 1636 1598 455
BsaHI Gr'CG_yC
Cuts at: 3157 3405 4107 5603 3157
Size: 248 702 1496 3280
Fragments arranged by size:
3280 1496 702 248
BsaWI w'CCGG_w
Cuts at: 848 2435 2915 3436 4114 4388 4535 4946 848
Size: 1587 480 521 678 274 147 411 1628
Fragments arranged by size:
1628 1587 678 521 480 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 250 280 759 789 250
Size: 30 479 30 5187
Fragments arranged by size:
5187 479 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 347 1321 347
Size: 974 4752
BseYI C'CCAG_C
Cuts at: 782 3151 3909 4486 4982 782
Size: 2369 758 577 496 1526
Fragments arranged by size:
2369 1526 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3268 3314 4522 104
Size: 3164 46 1208 1308
Fragments arranged by size:
3164 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2039 2085 2251 2419 2740 3518 3708 4500 2039
Size: 46 166 168 321 778 190 792 3265
Fragments arranged by size:
3265 792 778 321 190 168 166 46
BsmI GAATG_Cn'
Cuts at: 550 858 1084 1501 1585 2062 2230 2398 550
Size: 308 226 417 84 477 168 168 3878
Fragments arranged by size:
3878 477 417 308 226 168 168 84
Bsp1286I G_dGCh'C
Cuts at: 2039 2085 2251 2419 2589 2740 3351 3444 3518
Size: 46 166 168 170 151 611 93 74
Cuts at: 3518 3708 3770 4500 2039
Size: 190 62 730 3265
Fragments arranged by size:
3265 730 611 190 170 168 166 151
93 74 62 46
BspEI T'CCGG_A
Cuts at: 4946 4946
Size: 5726
BspHI T'CATG_A
Cuts at: 555 1014 4902 555
Size: 459 3888 1379
Fragments arranged by size:
3888 1379 459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2776 2990 3292 3673 2776
Size: 214 302 381 4829
Fragments arranged by size:
4829 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4018 4072 5556 5721 50
Size: 3968 54 1484 165 55
Fragments arranged by size:
3968 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3638 3638
Size: 5726
BsrFI r'CCGG_y
Cuts at: 1430 1514 3724 3905 4114 1430
Size: 84 2210 181 209 3042
Fragments arranged by size:
3042 2210 209 181 84
BsrGI T'GTAC_A
Cuts at: 2694 2694
Size: 5726
BssSI C'ACGA_G
Cuts at: 1705 1846 3997 4355 1705
Size: 141 2151 358 3076
Fragments arranged by size:
3076 2151 358 141
BstAPI GCAn_nnn'nTGC
Cuts at: 3103 3103
Size: 5726
BstBI TT'CG_AA
Cuts at: 377 4087 377
Size: 3710 2016
BstXI CCAn_nnnn'nTGG
Cuts at: 277 277
Size: 5726
BstYI r'GATC_y
Cuts at: 1 288 1100 1127 1364 1754 2751 3218 3241
Size: 287 812 27 237 390 997 467 23
Cuts at: 3241 3576 3822 4834 4920 4932 5278 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 997 812 467 449 390 346 335
287 246 237 86 27 23 12
BtgI C'CryG_G
Cuts at: 107 539 596 611 107
Size: 432 57 15 5222
Fragments arranged by size:
5222 432 57 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3676 3857 4020 3676
Size: 181 163 5382
Fragments arranged by size:
5382 181 163
BtsI GCAGTG_nn'
Cuts at: 288 1413 2001 2969 288
Size: 1125 588 968 3045
Fragments arranged by size:
3045 1125 968 588
DraI TTT'AAA
Cuts at: 2046 2130 2725 2046
Size: 84 595 5047
Fragments arranged by size:
5047 595 84
DrdI GACnn_nn'nnGTC
Cuts at: 3432 4290 3432
Size: 858 4868
EaeI y'GGCC_r
Cuts at: 1908 3311 3485 3876 3903 5248 1908
Size: 1403 174 391 27 1345 2386
Fragments arranged by size:
2386 1403 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3311 3311
Size: 5726
EarI CTCTTCn'nnn_
Cuts at: 238 1298 3749 3959 238
Size: 1060 2451 210 2005
Fragments arranged by size:
2451 2005 1060 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 3934 4244 4390 5536 2335
Size: 1599 310 146 1146 2525
Fragments arranged by size:
2525 1599 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2249 2417 2738 2249
Size: 168 321 5237
Fragments arranged by size:
5237 321 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 744 989 1322 1326 1717 1999 2055 2135
Size: 431 245 333 4 391 282 56 80
Cuts at: 2135 2387 3551 3983 4730 4998 313
Size: 252 1164 432 747 268 1041
Fragments arranged by size:
1164 1041 747 432 431 391 333 282
268 252 245 80 56 4
EcoNI CCTnn'n_nnAGG
Cuts at: 302 302
Size: 5726
EcoO109I rG'GnC_Cy
Cuts at: 228 2865 228
Size: 2637 3089
EcoRI G'AATT_C
Cuts at: 1460 2273 2357 2525 2730 1460
Size: 813 84 168 205 4456
Fragments arranged by size:
4456 813 205 168 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 3745 441
Size: 3304 2422
FspI TGC'GCA
Cuts at: 3507 3507
Size: 5726
HaeII r_GCGC'y
Cuts at: 3160 3408 4430 5409 3160
Size: 248 1022 979 3477
Fragments arranged by size:
3477 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 397 429 759 791 1045 1077 1119 1151 1521
Size: 32 330 32 254 32 42 32 370
Cuts at: 1521 1553 1746 1778 2616 2648 3568 3598 3600
Size: 32 193 32 838 32 920 30 2
Cuts at: 3600 3630 397
Size: 30 2493
Fragments arranged by size:
2493 920 838 370 330 254 193 42
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2641 2765 2641
Size: 124 5602
HindIII A'AGCT_T
Cuts at: 756 2570 756
Size: 1814 3912
KasI G'GCGC_C
Cuts at: 3156 3404 3156
Size: 248 5478
KpnI G_GTAC'C
Cuts at: 2746 2746
Size: 5726
MfeI C'AATT_G
Cuts at: 219 219
Size: 5726
MluI A'CGCG_T
Cuts at: 5159 5159
Size: 5726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 713 1221 4397 4581 194
Size: 519 508 3176 184 1339
Fragments arranged by size:
3176 1339 519 508 184
MscI TGG'CCA
Cuts at: 1910 3487 1910
Size: 1577 4149
MslI CAynn'nnrTG
Cuts at: 275 735 945 1445 1653 1709 2282 2690 3715
Size: 460 210 500 208 56 573 408 1025
Cuts at: 3715 3842 275
Size: 127 2159
Fragments arranged by size:
2159 1025 573 500 460 408 210 208
127 56
MspA1I CmG'CkG
Cuts at: 2837 3151 3511 4524 4769 2837
Size: 314 360 1013 245 3794
Fragments arranged by size:
3794 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1516 3907 1516
Size: 2391 3335
NarI GG'CG_CC
Cuts at: 3157 3405 3157
Size: 248 5478
NcoI C'CATG_G
Cuts at: 107 611 107
Size: 504 5222
NdeI CA'TA_TG
Cuts at: 1650 1650
Size: 5726
NgoMIV G'CCGG_C
Cuts at: 1514 3905 1514
Size: 2391 3335
NspI r_CATG'y
Cuts at: 2779 3279 4186 2779
Size: 500 907 4319
Fragments arranged by size:
4319 907 500
PciI A'CATG_T
Cuts at: 4182 4182
Size: 5726
PmeI GTTT'AAAC
Cuts at: 2725 2725
Size: 5726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4865 4897 4865
Size: 32 5694
PpuMI rG'GwC_Cy
Cuts at: 228 228
Size: 5726
PsiI TTA'TAA
Cuts at: 1316 1316
Size: 5726
PspXI vC'TCGA_Gb
Cuts at: 2670 2670
Size: 5726
PstI C_TGCA'G
Cuts at: 1828 2773 3458 1828
Size: 945 685 4096
Fragments arranged by size:
4096 945 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5726
PvuII CAG'CTG
Cuts at: 3151 3511 3151
Size: 360 5366
RsrII CG'GwC_CG
Cuts at: 3921 3921
Size: 5726
SacI G_AGCT'C
Cuts at: 2251 2419 2740 2251
Size: 168 321 5237
Fragments arranged by size:
5237 321 168
SalI G'TCGA_C
Cuts at: 2763 2763
Size: 5726
SapI GCTCTTCn'nnn_
Cuts at: 1298 3749 3959 1298
Size: 2451 210 3065
Fragments arranged by size:
3065 2451 210
SbfI CC_TGCA'GG
Cuts at: 2773 2773
Size: 5726
ScaI AGT'ACT
Cuts at: 1917 5243 1917
Size: 3326 2400
SexAI A'CCwGG_T
Cuts at: 466 466
Size: 5726
SfcI C'TryA_G
Cuts at: 33 1473 1566 1824 2463 2769 3454 4447 4638
Size: 1440 93 258 639 306 685 993 191
Cuts at: 4638 33
Size: 1121
Fragments arranged by size:
1440 1121 993 685 639 306 258 191
93
SfoI GGC'GCC
Cuts at: 3158 3406 3158
Size: 248 5478
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5726
SmaI CCC'GGG
Cuts at: 2748 2748
Size: 5726
SmlI C'TyrA_G
Cuts at: 130 1179 2670 2881 3796 4288 4550 130
Size: 1049 1491 211 915 492 262 1306
Fragments arranged by size:
1491 1306 1049 915 492 262 211
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5726
SphI G_CATG'C
Cuts at: 2779 3279 2779
Size: 500 5226
StuI AGG'CCT
Cuts at: 1423 2320 1423
Size: 897 4829
StyI C'CwwG_G
Cuts at: 107 155 416 611 2860 3214 107
Size: 48 261 195 2249 354 2619
Fragments arranged by size:
2619 2249 354 261 195 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 287 1019 1023 2173 2425 2632 3309 3442 3901
Size: 732 4 1150 252 207 677 133 459
Cuts at: 3901 4110 287
Size: 209 1903
Fragments arranged by size:
1903 1150 732 677 459 252 209 207
133 4
TatI w'GTAC_w
Cuts at: 937 1915 2694 5241 937
Size: 978 779 2547 1422
Fragments arranged by size:
2547 1422 978 779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1727 1759 5243 5275 1727
Size: 32 3484 32 2178
Fragments arranged by size:
3484 2178 32 32
Tth111I GACn'n_nGTC
Cuts at: 575 2149 3523 575
Size: 1574 1374 2778
Fragments arranged by size:
2778 1574 1374
XbaI T'CTAG_A
Cuts at: 1948 2757 1948
Size: 809 4917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3221 3221
Size: 5726
XhoI C'TCGA_G
Cuts at: 2670 2670
Size: 5726
XmaI C'CCGG_G
Cuts at: 2746 2746
Size: 5726
XmnI GAAnn'nnTTC
Cuts at: 500 757 1339 1423 2236 2320 2404 500
Size: 257 582 84 813 84 84 3822
Fragments arranged by size:
3822 813 582 257 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI