Restriction map for
pF1KA1119
(Circular) (Six-base) MAPSORT of: pF1KA1119.seq Check: 1922 from: 1 to: 8660
pF1KA1119 8660 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1284 2543 3489 4777 1284
Size: 1259 946 1288 5167
Fragments arranged by size:
5167 1288 1259 946
AatII G_ACGT'C
Cuts at: 4750 5219 4750
Size: 469 8191
AccI GT'mk_AC
Cuts at: 1175 5698 1175
Size: 4523 4137
Acc65I G'GTAC_C
Cuts at: 800 3308 5676 800
Size: 2508 2368 3784
Fragments arranged by size:
3784 2508 2368
AclI AA'CG_TT
Cuts at: 8500 8500
Size: 8660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1867 2488 2836 3013 3571 3730 3766 4234 4411
Size: 621 348 177 558 159 36 468 177
Cuts at: 4411 4810 6485 6917 7664 7932 1867
Size: 399 1675 432 747 268 2595
Fragments arranged by size:
2595 1675 747 621 558 468 432 399
348 268 177 177 159 36
AfeI AGC'GCT
Cuts at: 5032 5032
Size: 8660
AflII C'TTAA_G
Cuts at: 4855 4855
Size: 8660
AflIII A'CryG_T
Cuts at: 4628 5558 6012 7116 8093 4628
Size: 930 454 1104 977 5195
Fragments arranged by size:
5195 1104 977 930 454
AgeI A'CCGG_T
Cuts at: 2222 7048 2222
Size: 4826 3834
AhdI GACnn_n'nnGTC
Cuts at: 472 472
Size: 8660
AleI CACnn'nnGTG
Cuts at: 5300 5300
Size: 8660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 1349 3097 3194 5254 298
Size: 1051 1748 97 2060 3704
Fragments arranged by size:
3704 2060 1748 1051 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5315 5347 5315
Size: 32 8628
AlwNI CAG_nnn'CTG
Cuts at: 2147 2754 4164 4587 4890 5349 5538 5965 7532
Size: 607 1410 423 303 459 189 427 1567
Cuts at: 7532 2147
Size: 3275
Fragments arranged by size:
3275 1567 1410 607 459 427 423 303
189
ApaI G_GGCC'C
Cuts at: 2592 3093 2592
Size: 501 8159
ApaLI G'TGCA_C
Cuts at: 135 3007 4672 7430 135
Size: 2872 1665 2758 1365
Fragments arranged by size:
2872 2758 1665 1365
ApoI r'AATT_y
Cuts at: 925 3241 4285 4860 5432 5461 5638 5664 8013
Size: 2316 1044 575 572 29 177 26 2349
Cuts at: 8013 8269 925
Size: 256 1316
Fragments arranged by size:
2349 2316 1316 1044 575 572 256 177
29 26
AseI AT'TA_AT
Cuts at: 20 437 3965 20
Size: 417 3528 4715
Fragments arranged by size:
4715 3528 417
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8660
AvaI C'yCGr_G
Cuts at: 2645 3871 4204 5680 2645
Size: 1226 333 1476 5625
Fragments arranged by size:
5625 1476 1226 333
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 402 435 402
Size: 33 8627
BamHI G'GATC_C
Cuts at: 149 5685 149
Size: 5536 3124
BanI G'GyrC_C
Cuts at: 800 3308 5676 6090 6338 6373 800
Size: 2508 2368 414 248 35 3087
Fragments arranged by size:
3087 2508 2368 414 248 35
BanII G_rGCy'C
Cuts at: 125 1683 2255 2592 3025 3093 3872 4469 5674
Size: 1558 572 337 433 68 779 597 1205
Cuts at: 5674 6704 125
Size: 1030 2081
Fragments arranged by size:
2081 1558 1205 1030 779 597 572 433
337 68
BbeI G_GCGC'C
Cuts at: 6094 6342 6094
Size: 248 8412
BbsI GAAGACnn'nnnn_
Cuts at: 619 849 910 1033 3537 619
Size: 230 61 123 2504 5742
Fragments arranged by size:
5742 2504 230 123 61
BbvCI CC'TCA_GC
Cuts at: 1013 3145 3766 5410 5531 1013
Size: 2132 621 1644 121 4142
Fragments arranged by size:
4142 2132 1644 621 121
BciVI GTATCCnnnnn_n'
Cuts at: 6558 7319 8660 6558
Size: 761 1341 6558
Fragments arranged by size:
6558 1341 761
BclI T'GATC_A
Cuts at: 1298 5174 6180 1298
Size: 3876 1006 3778
Fragments arranged by size:
3876 3778 1006
BglI GCCn_nnn'nGGC
Cuts at: 767 2596 4024 767
Size: 1829 1428 5403
Fragments arranged by size:
5403 1829 1428
BglII A'GATC_T
Cuts at: 2342 3107 3690 4910 6175 8212 2342
Size: 765 583 1220 1265 2037 2790
Fragments arranged by size:
2790 2037 1265 1220 765 583
BlpI GC'TnA_GC
Cuts at: 177 1133 2528 2841 4145 4199 5772 177
Size: 956 1395 313 1304 54 1573 3065
Fragments arranged by size:
3065 1573 1395 1304 956 313 54
Bme1580I G_kGCm'C
Cuts at: 139 2592 3011 3093 4597 4604 4676 6285 6378
Size: 2453 419 82 1504 7 72 1609 93
Cuts at: 6378 7434 139
Size: 1056 1365
Fragments arranged by size:
2453 1609 1504 1365 1056 419 93 82
72 7
BmrI ACTGGGnnnn_n'
Cuts at: 1332 1686 5872 5953 6288 8432 1332
Size: 354 4186 81 335 2144 1560
Fragments arranged by size:
4186 2144 1560 354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1803 2856 7027 1803
Size: 1053 4171 3436
Fragments arranged by size:
4171 3436 1053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 409 623 1477 1546 1689 1822 2314 2395
Size: 156 214 854 69 143 133 492 81
Cuts at: 2395 2548 3187 3310 3377 3478 3538 3598 3631
Size: 153 639 123 67 101 60 60 33
Cuts at: 3631 3745 3789 4159 4186 4540 5022 253
Size: 114 44 370 27 354 482 3891
Fragments arranged by size:
3891 854 639 492 482 370 354 214
156 153 143 133 123 114 101 81
69 67 60 60 44 33 27
Bpu10I CC'TnA_GC
Cuts at: 337 1013 1180 3145 3766 5410 5531 337
Size: 676 167 1965 621 1644 121 3466
Fragments arranged by size:
3466 1965 1644 676 621 167 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 2782 3835 4177 4651 5287 5836 6715 7207
Size: 1407 1053 342 474 636 549 879 492
Cuts at: 7207 7505 1375
Size: 298 2530
Fragments arranged by size:
2530 1407 1053 879 636 549 492 474
342 298
BsaI GGTCTCn'nnnn_
Cuts at: 1258 2035 8364 1258
Size: 777 6329 1554
Fragments arranged by size:
6329 1554 777
BsaAI yAC'GTr
Cuts at: 2718 6013 2718
Size: 3295 5365
BsaBI GATnn'nnATC
Cuts at: 6 1840 2170 6174 8211 6
Size: 1834 330 4004 2037 455
Fragments arranged by size:
4004 2037 1834 455 330
BsaHI Gr'CG_yC
Cuts at: 3119 4747 5216 6091 6339 7041 8537 3119
Size: 1628 469 875 248 702 1496 3242
Fragments arranged by size:
3242 1628 1496 875 702 469 248
BsaWI w'CCGG_w
Cuts at: 2222 2399 2789 4553 5024 5849 6370 7048 7322
Size: 177 390 1764 471 825 521 678 274
Cuts at: 7322 7469 7880 2222
Size: 147 411 3002
Fragments arranged by size:
3002 1764 825 678 521 471 411 390
274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1687 1717 1687
Size: 30 8630
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 995 1039 3070 3262 3307 3310 3880 3901 4189
Size: 44 2031 192 45 3 570 21 288
Cuts at: 4189 4192 4234 4537 5025 5284 995
Size: 3 42 303 488 259 4371
Fragments arranged by size:
4371 2031 570 488 303 288 259 192
45 44 42 21 3 3
BseYI C'CCAG_C
Cuts at: 1617 5342 5417 5619 6085 6843 7420 7916 1617
Size: 3725 75 202 466 758 577 496 2361
Fragments arranged by size:
3725 2361 758 577 496 466 202 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 971 1363 2152 2198 3625 971
Size: 392 789 46 1427 6006
Fragments arranged by size:
6006 1427 789 392 46
BsiEI CG_ry'CG
Cuts at: 104 6202 6248 7456 104
Size: 6098 46 1208 1308
Fragments arranged by size:
6098 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 125 139 1214 1229 1364 1991 2255 2534 2564
Size: 14 1075 15 135 627 264 279 30
Cuts at: 2564 2693 3011 3872 3896 4469 4676 5233 5674
Size: 129 318 861 24 573 207 557 441
Cuts at: 5674 6452 6642 7434 125
Size: 778 190 792 1351
Fragments arranged by size:
1351 1075 861 792 778 627 573 557
441 318 279 264 207 190 135 129
30 24 15 14
BsmI GAATG_Cn'
Cuts at: 2055 3690 4988 5100 2055
Size: 1635 1298 112 5615
Fragments arranged by size:
5615 1635 1298 112
BsmBI CGTCTCn'nnnn_
Cuts at: 2155 2866 2155
Size: 711 7949
Bsp1286I G_dGCh'C
Cuts at: 125 139 1214 1229 1364 1683 1991 2255 2534
Size: 14 1075 15 135 319 308 264 279
Cuts at: 2534 2564 2592 2693 3011 3025 3093 3872 3896
Size: 30 28 101 318 14 68 779 24
Cuts at: 3896 4469 4597 4604 4676 5233 5674 6285 6378
Size: 573 128 7 72 557 441 611 93
Cuts at: 6378 6452 6642 6704 7434 125
Size: 74 190 62 730 1351
Fragments arranged by size:
1351 1075 779 730 611 573 557 441
319 318 308 279 264 190 135 128
101 93 74 72 68 62 30 28
24 15 14 14 7
BspEI T'CCGG_A
Cuts at: 2399 2789 4553 7880 2399
Size: 390 1764 3327 3179
Fragments arranged by size:
3327 3179 1764 390
BspHI T'CATG_A
Cuts at: 348 1884 2414 3275 4817 7836 348
Size: 1536 530 861 1542 3019 1172
Fragments arranged by size:
3019 1542 1536 1172 861 530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1284 2273 2543 3489 4467 4777 5139 5207 5710
Size: 989 270 946 978 310 362 68 503
Cuts at: 5710 5924 6226 6607 1284
Size: 214 302 381 3337
Fragments arranged by size:
3337 989 978 946 503 381 362 310
302 270 214 68
BsrBI CCG'CTC
Cuts at: 50 2551 2840 2988 5520 6952 7006 8490 8655
Size: 2501 289 148 2532 1432 54 1484 165
Cuts at: 8655 50
Size: 55
Fragments arranged by size:
2532 2501 1484 1432 289 165 148 55
54
BsrDI GCAATG_nn'
Cuts at: 753 6572 753
Size: 5819 2841
BsrFI r'CCGG_y
Cuts at: 913 1697 2222 3940 5017 6658 6839 7048 913
Size: 784 525 1718 1077 1641 181 209 2525
Fragments arranged by size:
2525 1718 1641 1077 784 525 209 181
BsrGI T'GTAC_A
Cuts at: 267 267
Size: 8660
BssHII G'CGCG_C
Cuts at: 1306 1306
Size: 8660
BssSI C'ACGA_G
Cuts at: 3862 6931 7289 3862
Size: 3069 358 5233
Fragments arranged by size:
5233 3069 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1190 4680 6037 1190
Size: 3490 1357 3813
Fragments arranged by size:
3813 3490 1357
BstBI TT'CG_AA
Cuts at: 1936 2169 7021 1936
Size: 233 4852 3575
Fragments arranged by size:
4852 3575 233
BstEII G'GTnAC_C
Cuts at: 1251 1251
Size: 8660
BstXI CCAn_nnnn'nTGG
Cuts at: 114 2198 4137 114
Size: 2084 1939 4637
Fragments arranged by size:
4637 2084 1939
BstYI r'GATC_y
Cuts at: 1 149 1553 2342 3107 3690 4910 5685 6152
Size: 148 1404 789 765 583 1220 775 467
Cuts at: 6152 6175 6510 6756 7768 7854 7866 8212 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1404 1220 1012 789 775 765 583 467
449 346 335 246 148 86 23 12
BstZ17I GTA'TAC
Cuts at: 1176 1176
Size: 8660
Bsu36I CC'TnA_GG
Cuts at: 2824 3043 3901 2824
Size: 219 858 7583
Fragments arranged by size:
7583 858 219
BtgI C'CryG_G
Cuts at: 2975 3530 3776 3810 5318 5600 2975
Size: 555 246 34 1508 282 6035
Fragments arranged by size:
6035 1508 555 282 246 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1225 6610 6791 6954 1225
Size: 5385 181 163 2931
Fragments arranged by size:
5385 2931 181 163
BtsI GCAGTG_nn'
Cuts at: 2135 4829 5189 5903 2135
Size: 2694 360 714 4892
Fragments arranged by size:
4892 2694 714 360
ClaI AT'CG_AT
Cuts at: 2909 2909
Size: 8660
DraI TTT'AAA
Cuts at: 930 4079 5438 5659 930
Size: 3149 1359 221 3931
Fragments arranged by size:
3931 3149 1359 221
DrdI GACnn_nn'nnGTC
Cuts at: 3541 6366 7224 3541
Size: 2825 858 4977
Fragments arranged by size:
4977 2825 858
EaeI y'GGCC_r
Cuts at: 492 557 665 2004 2331 3779 6245 6419 6810
Size: 65 108 1339 327 1448 2466 174 391
Cuts at: 6810 6837 8182 492
Size: 27 1345 970
Fragments arranged by size:
2466 1448 1345 1339 970 391 327 174
108 65 27
EagI C'GGCC_G
Cuts at: 6245 6245
Size: 8660
EarI CTCTTCn'nnn_
Cuts at: 680 1823 2239 3556 3610 3706 5170 6683 6893
Size: 1143 416 1317 54 96 1464 1513 210
Cuts at: 6893 680
Size: 2447
Fragments arranged by size:
2447 1513 1464 1317 1143 416 210 96
54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3420 3664 5074 6868 7178 7324 8470 3420
Size: 244 1410 1794 310 146 1146 3610
Fragments arranged by size:
3610 1794 1410 1146 310 244 146
EcoICRI GAG'CTC
Cuts at: 123 2253 3870 4467 5672 123
Size: 2130 1617 597 1205 3111
Fragments arranged by size:
3111 2130 1617 1205 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 409 623 1477 1546 1689 1822 1867 2314
Size: 156 214 854 69 143 133 45 447
Cuts at: 2314 2395 2488 2548 2836 3013 3187 3310 3377
Size: 81 93 60 288 177 174 123 67
Cuts at: 3377 3478 3538 3571 3598 3631 3730 3745 3766
Size: 101 60 33 27 33 99 15 21
Cuts at: 3766 3789 4159 4186 4234 4411 4540 4810 5022
Size: 23 370 27 48 177 129 270 212
Cuts at: 5022 6485 6917 7664 7932 253
Size: 1463 432 747 268 981
Fragments arranged by size:
1463 981 854 747 447 432 370 288
270 268 214 212 177 177 174 156
143 133 129 123 101 99 93 81
69 67 60 60 48 45 33 33
27 27 23 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1291 2305 2604 3322 3496 3520 4216 1291
Size: 1014 299 718 174 24 696 5735
Fragments arranged by size:
5735 1014 718 696 299 174 24
EcoO109I rG'GnC_Cy
Cuts at: 1374 1413 1653 1896 2310 2588 2679 2820 3089
Size: 39 240 243 414 278 91 141 269
Cuts at: 3089 3905 4209 4416 4542 5799 1374
Size: 816 304 207 126 1257 4235
Fragments arranged by size:
4235 1257 816 414 304 278 269 243
240 207 141 126 91 39
EcoRI G'AATT_C
Cuts at: 4285 5638 5664 4285
Size: 1353 26 7281
Fragments arranged by size:
7281 1353 26
EcoRV GAT'ATC
Cuts at: 306 306
Size: 8660
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2121 2364 6679 2121
Size: 243 4315 4102
Fragments arranged by size:
4315 4102 243
FspI TGC'GCA
Cuts at: 3073 3880 6441 3073
Size: 807 2561 5292
Fragments arranged by size:
5292 2561 807
HaeII r_GCGC'y
Cuts at: 177 3923 4461 5034 6094 6342 7364 8343 177
Size: 3746 538 573 1060 248 1022 979 494
Fragments arranged by size:
3746 1060 1022 979 573 538 494 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 458 490 1687 1719 2935 2967 3286 3318 3712
Size: 32 1197 32 1216 32 319 32 394
Cuts at: 3712 3744 4732 4764 5206 5238 6502 6532 6534
Size: 32 988 32 442 32 1264 30 2
Cuts at: 6534 6564 458
Size: 30 2554
Fragments arranged by size:
2554 1264 1216 1197 988 442 394 319
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 188 4005 5699 188
Size: 3817 1694 3149
Fragments arranged by size:
3817 3149 1694
HpaI GTT'AAC
Cuts at: 188 188
Size: 8660
KasI G'GCGC_C
Cuts at: 6090 6338 6090
Size: 248 8412
KpnI G_GTAC'C
Cuts at: 804 3312 5680 804
Size: 2508 2368 3784
Fragments arranged by size:
3784 2508 2368
MfeI C'AATT_G
Cuts at: 260 260
Size: 8660
MluI A'CGCG_T
Cuts at: 8093 8093
Size: 8660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 419 1616 1763 2003 2138 3320 4787 4955 7331
Size: 1197 147 240 135 1182 1467 168 2376
Cuts at: 7331 7515 419
Size: 184 1564
Fragments arranged by size:
2376 1564 1467 1197 1182 240 184 168
147 135
MscI TGG'CCA
Cuts at: 494 559 2006 6421 494
Size: 65 1447 4415 2733
Fragments arranged by size:
4415 2733 1447 65
MslI CAynn'nnrTG
Cuts at: 112 1331 2196 2413 2656 3535 4730 5236 5300
Size: 1219 865 217 243 879 1195 506 64
Cuts at: 5300 6649 6776 112
Size: 1349 127 1996
Fragments arranged by size:
1996 1349 1219 1195 879 865 506 243
217 127 64
MspA1I CmG'CkG
Cuts at: 181 282 2075 2182 2751 2895 2901 3609 3930
Size: 101 1793 107 569 144 6 708 321
Cuts at: 3930 4149 4491 4802 5771 6085 6445 7458 7703
Size: 219 342 311 969 314 360 1013 245
Cuts at: 7703 181
Size: 1138
Fragments arranged by size:
1793 1138 1013 969 708 569 360 342
321 314 311 245 219 144 107 101
6
NaeI GCC'GGC
Cuts at: 3942 6841 3942
Size: 2899 5761
NarI GG'CG_CC
Cuts at: 6091 6339 6091
Size: 248 8412
NcoI C'CATG_G
Cuts at: 2975 3530 5318 5600 2975
Size: 555 1788 282 6035
Fragments arranged by size:
6035 1788 555 282
NgoMIV G'CCGG_C
Cuts at: 3940 6839 3940
Size: 2899 5761
NspI r_CATG'y
Cuts at: 1516 1737 2583 4003 4518 4632 5241 5562 5713
Size: 221 846 1420 515 114 609 321 151
Cuts at: 5713 6213 7120 1516
Size: 500 907 3056
Fragments arranged by size:
3056 1420 907 846 609 515 500 321
221 151 114
PasI CC'CwG_GG
Cuts at: 1918 1918
Size: 8660
PciI A'CATG_T
Cuts at: 4628 5558 7116 4628
Size: 930 1558 6172
Fragments arranged by size:
6172 1558 930
PflMI CCAn_nnn'nTGG
Cuts at: 463 501 3501 4164 4437 5127 463
Size: 38 3000 663 273 690 3996
Fragments arranged by size:
3996 3000 690 663 273 38
PfoI T'CCnGG_A
Cuts at: 386 1415 2291 386
Size: 1029 876 6755
Fragments arranged by size:
6755 1029 876
PmeI GTTT'AAAC
Cuts at: 5659 5659
Size: 8660
PmlI CAC'GTG
Cuts at: 2718 2718
Size: 8660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7799 7831 7799
Size: 32 8628
PpuMI rG'GwC_Cy
Cuts at: 1413 1653 1896 2310 2679 3905 4416 1413
Size: 240 243 414 369 1226 511 5657
Fragments arranged by size:
5657 1226 511 414 369 243 240
PshAI GACnn'nnGTC
Cuts at: 1270 1270
Size: 8660
PspOMI G'GGCC_C
Cuts at: 2588 3089 2588
Size: 501 8159
PspXI vC'TCGA_Gb
Cuts at: 3871 4204 3871
Size: 333 8327
PstI C_TGCA'G
Cuts at: 360 1292 2284 2447 2492 2900 3053 3086 3560
Size: 932 992 163 45 408 153 33 474
Cuts at: 3560 4154 5150 5707 6392 360
Size: 594 996 557 685 2628
Fragments arranged by size:
2628 996 992 932 685 594 557 474
408 163 153 45 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8660
PvuII CAG'CTG
Cuts at: 181 282 2182 2895 3609 4149 6085 6445 181
Size: 101 1900 713 714 540 1936 360 2396
Fragments arranged by size:
2396 1936 1900 714 713 540 360 101
RsrII CG'GwC_CG
Cuts at: 6855 6855
Size: 8660
SacI G_AGCT'C
Cuts at: 125 2255 3872 4469 5674 125
Size: 2130 1617 597 1205 3111
Fragments arranged by size:
3111 2130 1617 1205 597
SalI G'TCGA_C
Cuts at: 5697 5697
Size: 8660
SapI GCTCTTCn'nnn_
Cuts at: 1823 5170 6683 6893 1823
Size: 3347 1513 210 3590
Fragments arranged by size:
3590 3347 1513 210
SbfI CC_TGCA'GG
Cuts at: 5150 5707 5150
Size: 557 8103
ScaI AGT'ACT
Cuts at: 8177 8177
Size: 8660
SexAI A'CCwGG_T
Cuts at: 4559 4559
Size: 8660
SfcI C'TryA_G
Cuts at: 33 126 220 356 486 1152 1288 2280 2443
Size: 93 94 136 130 666 136 992 163
Cuts at: 2443 2488 2896 3049 3082 3556 3828 4150 5146
Size: 45 408 153 33 474 272 322 996
Cuts at: 5146 5703 6388 7381 7572 33
Size: 557 685 993 191 1121
Fragments arranged by size:
1121 996 993 992 685 666 557 474
408 322 272 191 163 153 136 136
130 94 93 45 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 2596 2596
Size: 8660
SfoI GGC'GCC
Cuts at: 6092 6340 6092
Size: 248 8412
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8660
SmaI CCC'GGG
Cuts at: 2647 5682 2647
Size: 3035 5625
SmlI C'TyrA_G
Cuts at: 1390 2797 3850 3871 4192 4204 4666 4855 5266
Size: 1407 1053 21 321 12 462 189 411
Cuts at: 5266 5815 6730 7222 7484 1390
Size: 549 915 492 262 2566
Fragments arranged by size:
2566 1407 1053 915 549 492 462 411
321 262 189 21 12
SpeI A'CTAG_T
Cuts at: 67 1866 67
Size: 1799 6861
SphI G_CATG'C
Cuts at: 2583 4518 5241 5713 6213 2583
Size: 1935 723 472 500 5030
Fragments arranged by size:
5030 1935 723 500 472
SspI AAT'ATT
Cuts at: 3331 3331
Size: 8660
StuI AGG'CCT
Cuts at: 2599 2638 4027 4115 2599
Size: 39 1389 88 7144
Fragments arranged by size:
7144 1389 88 39
StyI C'CwwG_G
Cuts at: 191 1110 1558 2975 3530 4110 5318 5600 5794
Size: 919 448 1417 555 580 1208 282 194
Cuts at: 5794 6148 191
Size: 354 2703
Fragments arranged by size:
2703 1417 1208 919 580 555 448 354
282 194
SwaI ATTT'AAAT
Cuts at: 4079 4079
Size: 8660
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5431 6243 6376 6835 7044 5431
Size: 812 133 459 209 7047
Fragments arranged by size:
7047 812 459 209 133
TatI w'GTAC_w
Cuts at: 267 773 4796 8175 267
Size: 506 4023 3379 752
Fragments arranged by size:
4023 3379 752 506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8177 8209 8177
Size: 32 8628
Tth111I GACn'n_nGTC
Cuts at: 1281 6457 1281
Size: 5176 3484
XbaI T'CTAG_A
Cuts at: 5691 5691
Size: 8660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 711 999 4326 6155 711
Size: 288 3327 1829 3216
Fragments arranged by size:
3327 3216 1829 288
XhoI C'TCGA_G
Cuts at: 3871 4204 3871
Size: 333 8327
XmaI C'CCGG_G
Cuts at: 2645 5680 2645
Size: 3035 5625
XmnI GAAnn'nnTTC
Cuts at: 245 4504 245
Size: 4259 4401
ZraI GAC'GTC
Cuts at: 4748 5217 4748
Size: 469 8191
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BcgI BfrBI BmgBI BmtI BsiWI CspCI
DraIII FseI FspAI HindIII NdeI NheI NotI NruI
NsiI PacI PsiI PsrI SacII SanDI SgrAI SnaBI
SrfI