Restriction map for
pF1KA1082
(Circular) (Six-base) MAPSORT of: pF1KA1082.seq Check: 4587 from: 1 to: 8396
pF1KA1082 8396 bp
With 182 enzymes: SgfI *
December 5, 2008 09:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1251 5301 1251
Size: 4050 4346
AccI GT'mk_AC
Cuts at: 1034 1138 3492 4436 5434 1034
Size: 104 2354 944 998 3996
Fragments arranged by size:
3996 2354 998 944 104
Acc65I G'GTAC_C
Cuts at: 2870 5063 5412 2870
Size: 2193 349 5854
Fragments arranged by size:
5854 2193 349
AclI AA'CG_TT
Cuts at: 8236 8236
Size: 8396
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2629 2800 3692 6221 6653 7006 7668 2629
Size: 171 892 2529 432 353 662 3357
Fragments arranged by size:
3357 2529 892 662 432 353 171
AflIII A'CryG_T
Cuts at: 5232 5748 7548 7829 5232
Size: 516 1800 281 5799
Fragments arranged by size:
5799 1800 516 281
AhdI GACnn_n'nnGTC
Cuts at: 3520 3520
Size: 8396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 4973 160
Size: 4813 3583
AlwNI CAG_nnn'CTG
Cuts at: 1423 1441 4457 5701 7139 1423
Size: 18 3016 1244 1438 2680
Fragments arranged by size:
3016 2680 1438 1244 18
ApaI G_GGCC'C
Cuts at: 1076 2251 2647 4720 1076
Size: 1175 396 2073 4752
Fragments arranged by size:
4752 2073 1175 396
ApaLI G'TGCA_C
Cuts at: 7234 7234
Size: 8396
ApoI r'AATT_y
Cuts at: 310 1488 1497 2615 4417 5263 5400 7749 8005
Size: 1178 9 1118 1802 846 137 2349 256
Cuts at: 8005 310
Size: 701
Fragments arranged by size:
2349 1802 1178 1118 846 701 256 137
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 8396
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8396
AvaI C'yCGr_G
Cuts at: 239 1479 1994 2131 3449 5416 239
Size: 1240 515 137 1318 1967 3219
Fragments arranged by size:
3219 1967 1318 1240 515 137
AvrII C'CTAG_G
Cuts at: 5136 5136
Size: 8396
BamHI G'GATC_C
Cuts at: 5421 5421
Size: 8396
BanI G'GyrC_C
Cuts at: 282 402 2118 2829 2870 3609 5063 5353 5412
Size: 120 1716 711 41 739 1454 290 59
Cuts at: 5412 5826 6074 6109 282
Size: 414 248 35 2569
Fragments arranged by size:
2569 1716 1454 739 711 414 290 248
120 59 41 35
BanII G_rGCy'C
Cuts at: 1076 1804 2230 2251 2647 2798 4391 4720 5169
Size: 728 426 21 396 151 1593 329 449
Cuts at: 5169 5410 6440 1076
Size: 241 1030 3032
Fragments arranged by size:
3032 1593 1030 728 449 426 396 329
241 151 21
BbeI G_GCGC'C
Cuts at: 406 5830 6078 406
Size: 5424 248 2724
Fragments arranged by size:
5424 2724 248
BbsI GAAGACnn'nnnn_
Cuts at: 4053 5219 4053
Size: 1166 7230
BbvCI CC'TCA_GC
Cuts at: 174 359 4074 174
Size: 185 3715 4496
Fragments arranged by size:
4496 3715 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2830 2864 4870 4904 2830
Size: 34 2006 34 6322
Fragments arranged by size:
6322 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6294 7350 8396 6294
Size: 1056 1046 6294
Fragments arranged by size:
6294 1056 1046
BclI T'GATC_A
Cuts at: 650 5034 5916 650
Size: 4384 882 3130
Fragments arranged by size:
4384 3130 882
BfrBI ATG'CAT
Cuts at: 1694 2819 1694
Size: 1125 7271
BglI GCCn_nnn'nGGC
Cuts at: 250 250
Size: 8396
BglII A'GATC_T
Cuts at: 3054 3667 4316 4617 5911 7948 3054
Size: 613 649 301 1294 2037 3502
Fragments arranged by size:
3502 2037 1294 649 613 301
BlpI GC'TnA_GC
Cuts at: 2229 2666 5508 2229
Size: 437 2842 5117
Fragments arranged by size:
5117 2842 437
Bme1580I G_kGCm'C
Cuts at: 287 1076 2251 2647 4720 6021 6114 7238 287
Size: 789 1175 396 2073 1301 93 1124 1445
Fragments arranged by size:
2073 1445 1301 1175 1124 789 396 93
BmrI ACTGGGnnnn_n'
Cuts at: 412 502 3243 5608 5689 6024 8168 412
Size: 90 2741 2365 81 335 2144 640
Fragments arranged by size:
2741 2365 2144 640 335 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1549 2157 6763 1549
Size: 608 4606 3182
Fragments arranged by size:
4606 3182 608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1898 2265 2526 2810 2884 3608 5183 5210
Size: 1486 367 261 284 74 724 1575 27
Cuts at: 5210 412
Size: 3598
Fragments arranged by size:
3598 1575 1486 724 367 284 261 74
27
Bpu10I CC'TnA_GC
Cuts at: 174 359 1798 1803 3283 4074 174
Size: 185 1439 5 1480 791 4496
Fragments arranged by size:
4496 1480 1439 791 185 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1810 4363 4546 4852 5344 5572 6451 7165 7463
Size: 2553 183 306 492 228 879 714 298
Cuts at: 7463 1810
Size: 2743
Fragments arranged by size:
2743 2553 879 714 492 306 298 228
183
BsaI GGTCTCn'nnnn_
Cuts at: 395 3055 3507 3909 3983 4418 8100 395
Size: 2660 452 402 74 435 3682 691
Fragments arranged by size:
3682 2660 691 452 435 402 74
BsaAI yAC'GTr
Cuts at: 5749 5749
Size: 8396
BsaBI GATnn'nnATC
Cuts at: 6 4834 5910 7947 6
Size: 4828 1076 2037 455
Fragments arranged by size:
4828 2037 1076 455
BsaHI Gr'CG_yC
Cuts at: 403 5827 6075 6777 8273 403
Size: 5424 248 702 1496 526
Fragments arranged by size:
5424 1496 702 526 248
BsaWI w'CCGG_w
Cuts at: 1877 2873 2933 5585 6106 6784 7195 7342 7616
Size: 996 60 2652 521 678 411 147 274
Cuts at: 7616 1877
Size: 2657
Fragments arranged by size:
2657 2652 996 678 521 411 274 147
60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 459 489 957 987 1896 1916 1926 1946 2524
Size: 30 468 30 909 20 10 20 578
Cuts at: 2524 2554 3422 3452 459
Size: 30 868 30 5403
Fragments arranged by size:
5403 909 868 578 468 30 30 30
30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 978 2076 2133 2190 2322 2362 2380 2596 3614
Size: 1098 57 57 132 40 18 216 1018
Cuts at: 3614 3753 5113 978
Size: 139 1360 4261
Fragments arranged by size:
4261 1360 1098 1018 216 139 132 57
57 40 18
BseYI C'CCAG_C
Cuts at: 2122 2640 2676 2990 3942 4294 5117 5821 6579
Size: 518 36 314 952 352 823 704 758
Cuts at: 6579 7244 7652 2122
Size: 665 408 2866
Fragments arranged by size:
2866 952 823 758 704 665 518 408
352 314 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1234 1625 2824 3757 5149 608
Size: 626 391 1199 933 1392 3855
Fragments arranged by size:
3855 1392 1199 933 626 391
BsiEI CG_ry'CG
Cuts at: 104 1471 5938 5984 7214 104
Size: 1367 4467 46 1230 1286
Fragments arranged by size:
4467 1367 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1530 1804 2627 3848 4391 4556 4851 5410 6188
Size: 274 823 1221 543 165 295 559 778
Cuts at: 6188 6378 7238 1530
Size: 190 860 2688
Fragments arranged by size:
2688 1221 860 823 778 559 543 295
274 190 165
BsmI GAATG_Cn'
Cuts at: 434 1095 2860 434
Size: 661 1765 5970
Fragments arranged by size:
5970 1765 661
Bsp1286I G_dGCh'C
Cuts at: 287 1076 1530 1804 2230 2251 2627 2647 2798
Size: 789 454 274 426 21 376 20 151
Cuts at: 2798 3848 4391 4556 4720 4851 5169 5410 6021
Size: 1050 543 165 164 131 318 241 611
Cuts at: 6021 6114 6188 6378 6440 7238 287
Size: 93 74 190 62 798 1445
Fragments arranged by size:
1445 1050 798 789 611 543 454 426
376 318 274 241 190 165 164 151
131 93 74 62 21 20
BspEI T'CCGG_A
Cuts at: 2933 2933
Size: 8396
BspHI T'CATG_A
Cuts at: 1959 4851 4917 5037 5049 5295 5367 6828 1959
Size: 2892 66 120 12 246 72 1461 3527
Fragments arranged by size:
3527 2892 1461 246 120 72 66 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1251 3525 5166 5301 5446 5660 5962 6343 1251
Size: 2274 1641 135 145 214 302 381 3304
Fragments arranged by size:
3304 2274 1641 381 302 214 145 135
BsrBI CCG'CTC
Cuts at: 50 212 275 2279 2868 6688 6742 8226 8391
Size: 162 63 2004 589 3820 54 1484 165
Cuts at: 8391 50
Size: 55
Fragments arranged by size:
3820 2004 1484 589 165 162 63 55
54
BsrDI GCAATG_nn'
Cuts at: 446 4124 6308 446
Size: 3678 2184 2534
Fragments arranged by size:
3678 2534 2184
BsrFI r'CCGG_y
Cuts at: 253 1466 2711 3278 3293 3606 6394 6575 253
Size: 1213 1245 567 15 313 2788 181 2074
Fragments arranged by size:
2788 2074 1245 1213 567 313 181 15
BsrGI T'GTAC_A
Cuts at: 4727 4727
Size: 8396
BssSI C'ACGA_G
Cuts at: 6667 7375 6667
Size: 708 7688
BstAPI GCAn_nnn'nTGC
Cuts at: 5773 5773
Size: 8396
BstBI TT'CG_AA
Cuts at: 6757 6757
Size: 8396
BstXI CCAn_nnnn'nTGG
Cuts at: 1585 3138 4420 1585
Size: 1553 1282 5561
Fragments arranged by size:
5561 1553 1282
BstYI r'GATC_y
Cuts at: 1 216 834 1412 1822 3054 3667 4236 4316
Size: 215 618 578 410 1232 613 569 80
Cuts at: 4316 4617 4979 5421 5888 5911 6246 6492 6798
Size: 301 362 442 467 23 335 246 306
Cuts at: 6798 6810 6896 7948 1
Size: 12 86 1052 449
Fragments arranged by size:
1232 1052 618 613 578 569 467 449
442 410 362 335 306 301 246 215
86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 3835 3835
Size: 8396
BtgI C'CryG_G
Cuts at: 107 194 1924 2264 107
Size: 87 1730 340 6239
Fragments arranged by size:
6239 1730 340 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1064 1529 6346 6527 6690 1064
Size: 465 4817 181 163 2770
Fragments arranged by size:
4817 2770 465 181 163
BtsI GCAGTG_nn'
Cuts at: 164 2667 3309 3893 3950 5342 5639 164
Size: 2503 642 584 57 1392 297 2921
Fragments arranged by size:
2921 2503 1392 642 584 297 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3220 3255 3220
Size: 35 8361
DraI TTT'AAA
Cuts at: 5395 5395
Size: 8396
DraIII CAC_nnn'GTG
Cuts at: 3304 3304
Size: 8396
DrdI GACnn_nn'nnGTC
Cuts at: 6102 7446 6102
Size: 1344 7052
EaeI y'GGCC_r
Cuts at: 457 957 1347 2274 2739 3080 3295 5981 6155
Size: 500 390 927 465 341 215 2686 174
Cuts at: 6155 6546 6573 7918 457
Size: 391 27 1345 935
Fragments arranged by size:
2686 1345 935 927 500 465 391 390
341 215 174 27
EagI C'GGCC_G
Cuts at: 5981 5981
Size: 8396
EarI CTCTTCn'nnn_
Cuts at: 925 1518 1994 2094 2188 3036 3226 3308 3573
Size: 593 476 100 94 848 190 82 265
Cuts at: 3573 3668 4081 4758 4853 6419 6629 925
Size: 95 413 677 95 1566 210 2692
Fragments arranged by size:
2692 1566 848 677 593 476 413 265
210 190 100 95 95 94 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3611 3756 5239 6604 7346 7492 8206 127
Size: 3484 145 1483 1365 742 146 714 317
Fragments arranged by size:
3484 1483 1365 742 714 317 146 145
EcoICRI GAG'CTC
Cuts at: 1802 4389 5408 1802
Size: 2587 1019 4790
Fragments arranged by size:
4790 2587 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1898 2265 2526 2629 2800 2810 2884 3608
Size: 1486 367 261 103 171 10 74 724
Cuts at: 3608 3692 5183 5210 6221 6653 7006 7668 412
Size: 84 1491 27 1011 432 353 662 1140
Fragments arranged by size:
1491 1486 1140 1011 724 662 432 367
353 261 171 103 84 74 27 10
EcoNI CCTnn'n_nnAGG
Cuts at: 427 5257 427
Size: 4830 3566
EcoO109I rG'GnC_Cy
Cuts at: 223 291 414 688 1072 1073 2247 2365 2644
Size: 68 123 274 384 1 1174 118 279
Cuts at: 2644 2700 3830 3946 4717 5535 223
Size: 56 1130 116 771 818 3084
Fragments arranged by size:
3084 1174 1130 818 771 384 279 274
123 118 116 68 56 1
EcoRI G'AATT_C
Cuts at: 1488 2615 5263 5400 1488
Size: 1127 2648 137 4484
Fragments arranged by size:
4484 2648 1127 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 409 2537 6415 409
Size: 2128 3878 2390
Fragments arranged by size:
3878 2390 2128
FseI GG_CCGG'CC
Cuts at: 2715 2715
Size: 8396
FspI TGC'GCA
Cuts at: 235 6177 235
Size: 5942 2454
HaeII r_GCGC'y
Cuts at: 406 5830 6078 7308 8079 406
Size: 5424 248 1230 771 723
Fragments arranged by size:
5424 1230 771 723 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 201 233 459 491 985 1017 2143 2175 2310
Size: 32 226 32 494 32 1126 32 135
Cuts at: 2310 2342 2524 2556 2934 2966 3245 3277 3506
Size: 32 182 32 378 32 279 32 229
Cuts at: 3506 3538 3652 3684 5206 5238 6238 6268 6270
Size: 32 114 32 1522 32 1000 30 2
Cuts at: 6270 6300 201
Size: 30 2297
Fragments arranged by size:
2297 1522 1126 1000 494 378 279 229
226 182 135 114 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3493 5435 3493
Size: 1942 6454
HindIII A'AGCT_T
Cuts at: 2514 3922 2514
Size: 1408 6988
KasI G'GCGC_C
Cuts at: 402 5826 6074 402
Size: 5424 248 2724
Fragments arranged by size:
5424 2724 248
KpnI G_GTAC'C
Cuts at: 2874 5067 5416 2874
Size: 2193 349 5854
Fragments arranged by size:
5854 2193 349
MfeI C'AATT_G
Cuts at: 4308 4874 4308
Size: 566 7830
MluI A'CGCG_T
Cuts at: 7829 7829
Size: 8396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3983 4312 4983 7155 7339 3983
Size: 329 671 2172 184 5040
Fragments arranged by size:
5040 2172 671 329 184
MscI TGG'CCA
Cuts at: 459 1349 2741 3082 6157 459
Size: 890 1392 341 3075 2698
Fragments arranged by size:
3075 2698 1392 890 341
MslI CAynn'nnrTG
Cuts at: 2317 2824 3136 4790 4955 6385 6512 2317
Size: 507 312 1654 165 1430 127 4201
Fragments arranged by size:
4201 1654 1430 507 312 165 127
MspA1I CmG'CkG
Cuts at: 196 205 1372 2126 2589 3912 5507 5821 6181
Size: 9 1167 754 463 1323 1595 314 360
Cuts at: 6181 6965 7210 196
Size: 784 245 1382
Fragments arranged by size:
1595 1382 1323 1167 784 754 463 360
314 245 9
NaeI GCC'GGC
Cuts at: 255 2713 3295 6577 255
Size: 2458 582 3282 2074
Fragments arranged by size:
3282 2458 2074 582
NarI GG'CG_CC
Cuts at: 403 5827 6075 403
Size: 5424 248 2724
Fragments arranged by size:
5424 2724 248
NcoI C'CATG_G
Cuts at: 107 2264 107
Size: 2157 6239
NdeI CA'TA_TG
Cuts at: 4513 4513
Size: 8396
NgoMIV G'CCGG_C
Cuts at: 253 2711 3293 6575 253
Size: 2458 582 3282 2074
Fragments arranged by size:
3282 2458 2074 582
NsiI A_TGCA'T
Cuts at: 1696 2821 1696
Size: 1125 7271
NspI r_CATG'y
Cuts at: 622 1698 3445 5236 5449 5949 7552 622
Size: 1076 1747 1791 213 500 1603 1466
Fragments arranged by size:
1791 1747 1603 1466 1076 500 213
PciI A'CATG_T
Cuts at: 5232 7548 5232
Size: 2316 6080
PflMI CCAn_nnn'nTGG
Cuts at: 3159 3159
Size: 8396
PfoI T'CCnGG_A
Cuts at: 4322 4982 4322
Size: 660 7736
PmeI GTTT'AAAC
Cuts at: 5395 5395
Size: 8396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 529 561 6842 6874 529
Size: 32 6281 32 2051
Fragments arranged by size:
6281 2051 32 32
PpuMI rG'GwC_Cy
Cuts at: 291 414 688 3830 3946 291
Size: 123 274 3142 116 4741
Fragments arranged by size:
4741 3142 274 123 116
PshAI GACnn'nnGTC
Cuts at: 4527 4527
Size: 8396
PspOMI G'GGCC_C
Cuts at: 1072 2247 2643 4716 1072
Size: 1175 396 2073 4752
Fragments arranged by size:
4752 2073 1175 396
PspXI vC'TCGA_Gb
Cuts at: 239 239
Size: 8396
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2204 2236 5311 5343 2204
Size: 32 3075 32 5257
Fragments arranged by size:
5257 3075 32 32
PstI C_TGCA'G
Cuts at: 1893 2151 2594 2807 4453 5312 5443 6128 1893
Size: 258 443 213 1646 859 131 685 4161
Fragments arranged by size:
4161 1646 859 685 443 258 213 131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8396
PvuII CAG'CTG
Cuts at: 1372 2126 2589 5821 6181 1372
Size: 754 463 3232 360 3587
Fragments arranged by size:
3587 3232 754 463 360
RsrII CG'GwC_CG
Cuts at: 6591 6591
Size: 8396
SacI G_AGCT'C
Cuts at: 1804 4391 5410 1804
Size: 2587 1019 4790
Fragments arranged by size:
4790 2587 1019
SacII CC_GC'GG
Cuts at: 197 197
Size: 8396
SalI G'TCGA_C
Cuts at: 3491 5433 3491
Size: 1942 6454
SanDI GG'GwC_CC
Cuts at: 3830 3946 3830
Size: 116 8280
SapI GCTCTTCn'nnn_
Cuts at: 1994 3668 4081 6419 6629 1994
Size: 1674 413 2338 210 3761
Fragments arranged by size:
3761 2338 1674 413 210
SbfI CC_TGCA'GG
Cuts at: 2807 5443 2807
Size: 2636 5760
ScaI AGT'ACT
Cuts at: 7913 7913
Size: 8396
SexAI A'CCwGG_T
Cuts at: 4606 5174 4606
Size: 568 7828
SfcI C'TryA_G
Cuts at: 33 693 1226 1265 1632 1889 2147 2590 2803
Size: 660 533 39 367 257 258 443 213
Cuts at: 2803 2909 4449 5308 5439 6124 7092 7283 33
Size: 106 1540 859 131 685 968 191 1146
Fragments arranged by size:
1540 1146 968 859 685 660 533 443
367 258 257 213 191 131 106 39
SfoI GGC'GCC
Cuts at: 404 5828 6076 404
Size: 5424 248 2724
Fragments arranged by size:
5424 2724 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8396
SmaI CCC'GGG
Cuts at: 3451 5418 3451
Size: 1967 6429
SmlI C'TyrA_G
Cuts at: 239 1789 4378 4561 4867 5359 5551 6466 7180
Size: 1550 2589 183 306 492 192 915 714
Cuts at: 7180 7442 239
Size: 262 1193
Fragments arranged by size:
2589 1550 1193 915 714 492 306 262
192 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8396
SphI G_CATG'C
Cuts at: 5449 5949 5449
Size: 500 7896
SspI AAT'ATT
Cuts at: 3426 3426
Size: 8396
StuI AGG'CCT
Cuts at: 1477 1808 2606 3735 4707 1477
Size: 331 798 1129 972 5166
Fragments arranged by size:
5166 1129 972 798 331
StyI C'CwwG_G
Cuts at: 107 2111 2264 2705 4031 4964 5136 5530 5884
Size: 2004 153 441 1326 933 172 394 354
Cuts at: 5884 107
Size: 2619
Fragments arranged by size:
2619 2004 1326 933 441 394 354 172
153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 977 1291 2136 3310 5979 6112 6571 6780 977
Size: 314 845 1174 2669 133 459 209 2593
Fragments arranged by size:
2669 2593 1174 845 459 314 209 133
TatI w'GTAC_w
Cuts at: 4727 7911 4727
Size: 3184 5212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 730 762 957 973 989 1005 1080 1112 1896
Size: 32 195 16 16 16 75 32 784
Cuts at: 1896 1916 1928 1948 7913 7945 730
Size: 20 12 20 5965 32 1181
Fragments arranged by size:
5965 1181 784 195 75 32 32 32
20 20 16 16 16 12
Tth111I GACn'n_nGTC
Cuts at: 418 6193 418
Size: 5775 2621
XbaI T'CTAG_A
Cuts at: 3057 5427 3057
Size: 2370 6026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3138 4943 5891 3138
Size: 1805 948 5643
Fragments arranged by size:
5643 1805 948
XhoI C'TCGA_G
Cuts at: 239 239
Size: 8396
XmaI C'CCGG_G
Cuts at: 3449 5416 3449
Size: 1967 6429
XmnI GAAnn'nnTTC
Cuts at: 524 2494 4318 4586 524
Size: 1970 1824 268 4334
Fragments arranged by size:
4334 1970 1824 268
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AloI AscI BaeI
BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
EcoRV FspAI HpaI NheI NotI NruI PacI PasI
PmlI PsiI SfiI SgrAI SnaBI SrfI SwaI ZraI