Restriction map for
pF1KA1079
(Circular) (Six-base) MAPSORT of: pF1KA1079.seq Check: 8331 from: 1 to: 7475
pF1KA1079 7475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 231 3666 4103 4301 231
Size: 3435 437 198 3405
Fragments arranged by size:
3435 3405 437 198
AccI GT'mk_AC
Cuts at: 1367 4199 4513 1367
Size: 2832 314 4329
Fragments arranged by size:
4329 2832 314
Acc65I G'GTAC_C
Cuts at: 2550 2592 4491 2550
Size: 42 1899 5534
Fragments arranged by size:
5534 1899 42
AclI AA'CG_TT
Cuts at: 7315 7315
Size: 7475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 685 793 1328 1414 2326 2393 2404 5300
Size: 18 108 535 86 912 67 11 2896
Cuts at: 5300 5732 6085 6747 667
Size: 432 353 662 1395
Fragments arranged by size:
2896 1395 912 662 535 432 353 108
86 67 18 11
AfeI AGC'GCT
Cuts at: 1817 3131 1817
Size: 1314 6161
AflII C'TTAA_G
Cuts at: 2353 2353
Size: 7475
AflIII A'CryG_T
Cuts at: 1974 2635 4827 6627 6908 1974
Size: 661 2192 1800 281 2541
Fragments arranged by size:
2541 2192 1800 661 281
AhdI GACnn_n'nnGTC
Cuts at: 3054 3054
Size: 7475
AleI CACnn'nnGTG
Cuts at: 3364 3364
Size: 7475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 221 2542 3560 221
Size: 2321 1018 4136
Fragments arranged by size:
4136 2321 1018
AlwNI CAG_nnn'CTG
Cuts at: 1521 1657 2692 3076 3710 4780 6218 1521
Size: 136 1035 384 634 1070 1438 2778
Fragments arranged by size:
2778 1438 1070 1035 634 384 136
ApaI G_GGCC'C
Cuts at: 1664 3868 4241 1664
Size: 2204 373 4898
Fragments arranged by size:
4898 2204 373
ApaLI G'TGCA_C
Cuts at: 1315 6313 1315
Size: 4998 2477
ApoI r'AATT_y
Cuts at: 340 2711 4479 6828 7084 340
Size: 2371 1768 2349 256 731
Fragments arranged by size:
2371 2349 1768 731 256
AseI AT'TA_AT
Cuts at: 20 280 20
Size: 260 7215
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7475
AvaI C'yCGr_G
Cuts at: 1990 3226 3250 3445 3468 3517 3532 3913 4144
Size: 1236 24 195 23 49 15 381 231
Cuts at: 4144 4495 1990
Size: 351 4970
Fragments arranged by size:
4970 1236 381 351 231 195 49 24
23 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2477 2510 2477
Size: 33 7442
BamHI G'GATC_C
Cuts at: 4500 4500
Size: 7475
BanI G'GyrC_C
Cuts at: 216 2550 2592 3256 4091 4491 4905 5153 5188
Size: 2334 42 664 835 400 414 248 35
Cuts at: 5188 216
Size: 2503
Fragments arranged by size:
2503 2334 835 664 414 400 248 42
35
BanII G_rGCy'C
Cuts at: 1664 1999 3446 3868 4241 4489 5519 1664
Size: 335 1447 422 373 248 1030 3620
Fragments arranged by size:
3620 1447 1030 422 373 335 248
BbeI G_GCGC'C
Cuts at: 220 4909 5157 220
Size: 4689 248 2538
Fragments arranged by size:
4689 2538 248
BbsI GAAGACnn'nnnn_
Cuts at: 395 414 2525 2909 3698 395
Size: 19 2111 384 789 4172
Fragments arranged by size:
4172 2111 789 384 19
BbvCI CC'TCA_GC
Cuts at: 4024 4072 4024
Size: 48 7427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1438 1472 3796 3830 90
Size: 34 1314 34 2324 34 3735
Fragments arranged by size:
3735 2324 1314 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 315 5373 6429 7475 315
Size: 5058 1056 1046 315
Fragments arranged by size:
5058 1056 1046 315
BclI T'GATC_A
Cuts at: 4995 4995
Size: 7475
BglII A'GATC_T
Cuts at: 4990 7027 4990
Size: 2037 5438
BlpI GC'TnA_GC
Cuts at: 1550 4417 4587 1550
Size: 2867 170 4438
Fragments arranged by size:
4438 2867 170
Bme1580I G_kGCm'C
Cuts at: 586 1319 1664 3176 3259 3868 4064 4241 5100
Size: 733 345 1512 83 609 196 177 859
Cuts at: 5100 5193 6317 586
Size: 93 1124 1744
Fragments arranged by size:
1744 1512 1124 859 733 609 345 196
177 93 83
BmrI ACTGGGnnnn_n'
Cuts at: 2039 2878 4687 4768 5103 7247 2039
Size: 839 1809 81 335 2144 2267
Fragments arranged by size:
2267 2144 1809 839 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5842 5842
Size: 7475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1252 1730 3064 3127 3603 3732 1022
Size: 230 478 1334 63 476 129 4765
Fragments arranged by size:
4765 1334 478 476 230 129 63
Bpu10I CC'TnA_GC
Cuts at: 1522 1882 4024 4072 4270 4306 1522
Size: 360 2142 48 198 36 4691
Fragments arranged by size:
4691 2142 360 198 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 1918 4651 5530 6244 6542 482
Size: 1436 2733 879 714 298 1415
Fragments arranged by size:
2733 1436 1415 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1112 1290 2776 4039 4210 7179 1112
Size: 178 1486 1263 171 2969 1408
Fragments arranged by size:
2969 1486 1408 1263 178 171
BsaAI yAC'GTr
Cuts at: 1977 2636 4828 1977
Size: 659 2192 4624
Fragments arranged by size:
4624 2192 659
BsaBI GATnn'nnATC
Cuts at: 6 4989 7026 6
Size: 4983 2037 455
Fragments arranged by size:
4983 2037 455
BsaHI Gr'CG_yC
Cuts at: 217 4906 5154 5856 7352 217
Size: 4689 248 702 1496 340
Fragments arranged by size:
4689 1496 702 340 248
BsaWI w'CCGG_w
Cuts at: 1623 4664 5185 5863 6274 6421 6695 1623
Size: 3041 521 678 411 147 274 2403
Fragments arranged by size:
3041 2403 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1410 1915 2794 3003 3475 3979 4262 1410
Size: 505 879 209 472 504 283 4623
Fragments arranged by size:
4623 879 505 504 472 283 209
BseYI C'CCAG_C
Cuts at: 178 411 453 1226 1470 1868 2627 3069 3671
Size: 233 42 773 244 398 759 442 602
Cuts at: 3671 4233 4278 4284 4900 5658 6323 6731 178
Size: 562 45 6 616 758 665 408 922
Fragments arranged by size:
922 773 759 758 665 616 602 562
442 408 398 244 233 45 42 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 285 868 877 2566 3759 227
Size: 58 583 9 1689 1193 3943
Fragments arranged by size:
3943 1689 1193 583 58 9
BsiEI CG_ry'CG
Cuts at: 104 3768 5017 5063 6293 104
Size: 3664 1249 46 1230 1286
Fragments arranged by size:
3664 1286 1249 1230 46
BsiHKAI G_wGCw'C
Cuts at: 803 1319 2521 4055 4489 5267 5457 6317 803
Size: 516 1202 1534 434 778 190 860 1961
Fragments arranged by size:
1961 1534 1202 860 778 516 434 190
BsmI GAATG_Cn'
Cuts at: 1435 2364 3729 1435
Size: 929 1365 5181
Fragments arranged by size:
5181 1365 929
BsmBI CGTCTCn'nnnn_
Cuts at: 3370 3370
Size: 7475
Bsp1286I G_dGCh'C
Cuts at: 586 803 1319 1664 1999 2521 3176 3259 3446
Size: 217 516 345 335 522 655 83 187
Cuts at: 3446 3868 4055 4064 4241 4489 5100 5193 5267
Size: 422 187 9 177 248 611 93 74
Cuts at: 5267 5457 5519 6317 586
Size: 190 62 798 1744
Fragments arranged by size:
1744 798 655 611 522 516 422 345
335 248 217 190 187 187 177 93
83 74 62 9
BspHI T'CATG_A
Cuts at: 5907 5907
Size: 7475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 965 1343 2112 2538 3666 4007 4022 4103
Size: 734 378 769 426 1128 341 15 81
Cuts at: 4103 4301 4421 4525 4739 5041 5422 231
Size: 198 120 104 214 302 381 2284
Fragments arranged by size:
2284 1128 769 734 426 381 378 341
302 214 198 120 104 81 15
BsrBI CCG'CTC
Cuts at: 50 3247 4274 5767 5821 7305 7470 50
Size: 3197 1027 1493 54 1484 165 55
Fragments arranged by size:
3197 1493 1484 1027 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2524 4444 5387 2524
Size: 1920 943 4612
Fragments arranged by size:
4612 1920 943
BsrFI r'CCGG_y
Cuts at: 120 169 1466 2921 3186 3340 5473 5654 120
Size: 49 1297 1455 265 154 2133 181 1941
Fragments arranged by size:
2133 1941 1455 1297 265 181 154 49
BssSI C'ACGA_G
Cuts at: 1608 5746 6454 1608
Size: 4138 708 2629
Fragments arranged by size:
4138 2629 708
BstAPI GCAn_nnn'nTGC
Cuts at: 882 891 1323 4059 4852 882
Size: 9 432 2736 793 3505
Fragments arranged by size:
3505 2736 793 432 9
BstBI TT'CG_AA
Cuts at: 2567 5836 2567
Size: 3269 4206
BstEII G'GTnAC_C
Cuts at: 766 766
Size: 7475
BstXI CCAn_nnnn'nTGG
Cuts at: 3627 4233 3627
Size: 606 6869
BstYI r'GATC_y
Cuts at: 1 1653 3148 4383 4500 4967 4990 5325 5571
Size: 1652 1495 1235 117 467 23 335 246
Cuts at: 5571 5877 5889 5975 7027 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1652 1495 1235 1052 467 449 335 306
246 117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1699 4290 1699
Size: 2591 4884
BtgI C'CryG_G
Cuts at: 857 1301 3281 3686 3788 857
Size: 444 1980 405 102 4544
Fragments arranged by size:
4544 1980 444 405 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1041 2902 3302 3359 3758 5425 5606 5769 1041
Size: 1861 400 57 399 1667 181 163 2747
Fragments arranged by size:
2747 1861 1667 400 399 181 163 57
BtsI GCAGTG_nn'
Cuts at: 180 1387 4138 4718 180
Size: 1207 2751 580 2937
Fragments arranged by size:
2937 2751 1207 580
DraI TTT'AAA
Cuts at: 2100 2957 4474 2100
Size: 857 1517 5101
Fragments arranged by size:
5101 1517 857
DraIII CAC_nnn'GTG
Cuts at: 4059 4059
Size: 7475
DrdI GACnn_nn'nnGTC
Cuts at: 1363 5181 6525 1363
Size: 3818 1344 2313
Fragments arranged by size:
3818 2313 1344
EaeI y'GGCC_r
Cuts at: 866 1683 3294 4121 5060 5234 5625 5652 6997
Size: 817 1611 827 939 174 391 27 1345
Cuts at: 6997 866
Size: 1344
Fragments arranged by size:
1611 1345 1344 939 827 817 391 174
27
EagI C'GGCC_G
Cuts at: 5060 5060
Size: 7475
EarI CTCTTCn'nnn_
Cuts at: 5498 5708 5498
Size: 210 7265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 147 3474 5683 6425 6571 7285 147
Size: 3327 2209 742 146 714 337
Fragments arranged by size:
3327 2209 742 714 337 146
EcoICRI GAG'CTC
Cuts at: 4487 4487
Size: 7475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 685 793 1022 1252 1328 1414 1730 2326
Size: 18 108 229 230 76 86 316 596
Cuts at: 2326 2393 2404 3064 3127 3603 3732 5300 5732
Size: 67 11 660 63 476 129 1568 432
Cuts at: 5732 6085 6747 667
Size: 353 662 1395
Fragments arranged by size:
1568 1395 662 660 596 476 432 353
316 230 229 129 108 86 76 67
63 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3043 3940 3043
Size: 897 6578
EcoO109I rG'GnC_Cy
Cuts at: 318 1518 1704 2753 3465 3864 3996 4237 4238
Size: 1200 186 1049 712 399 132 241 1
Cuts at: 4238 4614 318
Size: 376 3179
Fragments arranged by size:
3179 1200 1049 712 399 376 241 186
132 1
EcoRI G'AATT_C
Cuts at: 4479 4479
Size: 7475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 579 903 5494 579
Size: 324 4591 2560
Fragments arranged by size:
4591 2560 324
FspI TGC'GCA
Cuts at: 787 5256 787
Size: 4469 3006
HaeII r_GCGC'y
Cuts at: 220 1819 1882 3133 4909 5157 6387 7158 220
Size: 1599 63 1251 1776 248 1230 771 537
Fragments arranged by size:
1776 1599 1251 1230 771 537 248 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 382 414 2737 2769 2943 2975 3040 3072 3225
Size: 32 2323 32 174 32 65 32 153
Cuts at: 3225 3257 3421 3439 3453 3471 4360 4392 5317
Size: 32 164 18 14 18 889 32 925
Cuts at: 5317 5347 5349 5379 382
Size: 30 2 30 2478
Fragments arranged by size:
2478 2323 925 889 174 164 153 65
32 32 32 32 32 32 30 30
18 18 14 2
HincII GTy'rAC
Cuts at: 1368 4442 4514 1368
Size: 3074 72 4329
Fragments arranged by size:
4329 3074 72
KasI G'GCGC_C
Cuts at: 216 4905 5153 216
Size: 4689 248 2538
Fragments arranged by size:
4689 2538 248
KpnI G_GTAC'C
Cuts at: 2554 2596 4495 2554
Size: 42 1899 5534
Fragments arranged by size:
5534 1899 42
MluI A'CGCG_T
Cuts at: 6908 6908
Size: 7475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 690 956 1193 1448 1728 4016 6234 6418 690
Size: 266 237 255 280 2288 2218 184 1747
Fragments arranged by size:
2288 2218 1747 280 266 255 237 184
MscI TGG'CCA
Cuts at: 5236 5236
Size: 7475
MslI CAynn'nnrTG
Cuts at: 2333 3364 5464 5591 2333
Size: 1031 2100 127 4217
Fragments arranged by size:
4217 2100 1031 127
MspA1I CmG'CkG
Cuts at: 859 1230 1303 1353 1682 3302 3388 3740 4120
Size: 371 73 50 329 1620 86 352 380
Cuts at: 4120 4586 4900 5260 6044 6289 859
Size: 466 314 360 784 245 2045
Fragments arranged by size:
2045 1620 784 466 380 371 360 352
329 314 245 86 73 50
NaeI GCC'GGC
Cuts at: 122 171 2923 3188 3342 5656 122
Size: 49 2752 265 154 2314 1941
Fragments arranged by size:
2752 2314 1941 265 154 49
NarI GG'CG_CC
Cuts at: 217 4906 5154 217
Size: 4689 248 2538
Fragments arranged by size:
4689 2538 248
NgoMIV G'CCGG_C
Cuts at: 120 169 2921 3186 3340 5654 120
Size: 49 2752 265 154 2314 1941
Fragments arranged by size:
2752 2314 1941 265 154 49
NspI r_CATG'y
Cuts at: 807 2448 4528 5028 6631 807
Size: 1641 2080 500 1603 1651
Fragments arranged by size:
2080 1651 1641 1603 500
PasI CC'CwG_GG
Cuts at: 3396 3396
Size: 7475
PciI A'CATG_T
Cuts at: 6627 6627
Size: 7475
PflMI CCAn_nnn'nTGG
Cuts at: 2742 2742
Size: 7475
PfoI T'CCnGG_A
Cuts at: 2654 2861 3041 2654
Size: 207 180 7088
Fragments arranged by size:
7088 207 180
PmeI GTTT'AAAC
Cuts at: 4474 4474
Size: 7475
PmlI CAC'GTG
Cuts at: 1977 2636 1977
Size: 659 6816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5921 5953 5921
Size: 32 7443
PpuMI rG'GwC_Cy
Cuts at: 318 1704 2753 3465 3996 318
Size: 1386 1049 712 531 3797
Fragments arranged by size:
3797 1386 1049 712 531
PshAI GACnn'nnGTC
Cuts at: 2681 2681
Size: 7475
PspOMI G'GGCC_C
Cuts at: 1660 3864 4237 1660
Size: 2204 373 4898
Fragments arranged by size:
4898 2204 373
PstI C_TGCA'G
Cuts at: 231 836 1080 1346 4522 5207 231
Size: 605 244 266 3176 685 2499
Fragments arranged by size:
3176 2499 685 605 266 244
PvuI CG_AT'CG
Cuts at: 104 3768 104
Size: 3664 3811
PvuII CAG'CTG
Cuts at: 1230 3388 4900 5260 1230
Size: 2158 1512 360 3445
Fragments arranged by size:
3445 2158 1512 360
RsrII CG'GwC_CG
Cuts at: 5670 5670
Size: 7475
SacI G_AGCT'C
Cuts at: 4489 4489
Size: 7475
SacII CC_GC'GG
Cuts at: 860 1304 860
Size: 444 7031
SalI G'TCGA_C
Cuts at: 1366 4512 1366
Size: 3146 4329
SanDI GG'GwC_CC
Cuts at: 1704 1704
Size: 7475
SapI GCTCTTCn'nnn_
Cuts at: 5498 5708 5498
Size: 210 7265
SbfI CC_TGCA'GG
Cuts at: 4522 4522
Size: 7475
ScaI AGT'ACT
Cuts at: 6992 6992
Size: 7475
SfcI C'TryA_G
Cuts at: 33 227 479 832 1076 1342 4518 5203 6171
Size: 194 252 353 244 266 3176 685 968
Cuts at: 6171 6362 33
Size: 191 1146
Fragments arranged by size:
3176 1146 968 685 353 266 252 244
194 191
SfoI GGC'GCC
Cuts at: 218 4907 5155 218
Size: 4689 248 2538
Fragments arranged by size:
4689 2538 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7475
SgrAI Cr'CCGG_yG
Cuts at: 120 120
Size: 7475
SmaI CCC'GGG
Cuts at: 3470 4497 3470
Size: 1027 6448
SmlI C'TyrA_G
Cuts at: 497 1897 2353 4630 5545 6259 6521 497
Size: 1400 456 2277 915 714 262 1451
Fragments arranged by size:
2277 1451 1400 915 714 456 262
SpeI A'CTAG_T
Cuts at: 67 3168 67
Size: 3101 4374
SphI G_CATG'C
Cuts at: 4528 5028 4528
Size: 500 6975
SspI AAT'ATT
Cuts at: 278 444 696 2060 278
Size: 166 252 1364 5693
Fragments arranged by size:
5693 1364 252 166
StyI C'CwwG_G
Cuts at: 3488 4609 4963 3488
Size: 1121 354 6000
Fragments arranged by size:
6000 1121 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 1102 1519 4063 5058 5191 5650 5859 754
Size: 348 417 2544 995 133 459 209 2370
Fragments arranged by size:
2544 2370 995 459 417 348 209 133
TatI w'GTAC_w
Cuts at: 1909 1936 6990 1909
Size: 27 5054 2394
Fragments arranged by size:
5054 2394 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6992 7024 6992
Size: 32 7443
Tth111I GACn'n_nGTC
Cuts at: 5272 5272
Size: 7475
XbaI T'CTAG_A
Cuts at: 4506 4506
Size: 7475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1467 2764 3831 4970 1467
Size: 1297 1067 1139 3972
Fragments arranged by size:
3972 1297 1139 1067
XmaI C'CCGG_G
Cuts at: 3468 4495 3468
Size: 1027 6448
XmnI GAAnn'nnTTC
Cuts at: 728 728
Size: 7475
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AloI AscI AvrII BfrBI BglI BmgBI
BmtI BsaXI BsiWI BspEI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PsiI PspXI
PsrI SexAI SfiI SnaBI SrfI StuI SwaI XhoI
ZraI