Restriction map for
pF1KA1065
(Circular) (Six-base) MAPSORT of: pF1KA1065.seq Check: 7082 from: 1 to: 5036
pF1KA1065 5036 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 1980 2074 1980
Size: 94 4942
Acc65I G'GTAC_C
Cuts at: 1255 2052 1255
Size: 797 4239
AclI AA'CG_TT
Cuts at: 4876 4876
Size: 5036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 925 1217 1431 2861 3293 4040 4308 445
Size: 480 292 214 1430 432 747 268 1173
Fragments arranged by size:
1430 1173 747 480 432 292 268 214
AflIII A'CryG_T
Cuts at: 378 1388 2388 3492 4469 378
Size: 1010 1000 1104 977 945
Fragments arranged by size:
1104 1010 1000 977 945
AgeI A'CCGG_T
Cuts at: 3424 3424
Size: 5036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 5036
AlwNI CAG_nnn'CTG
Cuts at: 423 774 1791 2341 3908 423
Size: 351 1017 550 1567 1551
Fragments arranged by size:
1567 1551 1017 550 351
ApaI G_GGCC'C
Cuts at: 211 543 744 1144 1174 1651 1948 211
Size: 332 201 400 30 477 297 3299
Fragments arranged by size:
3299 477 400 332 297 201 30
ApaLI G'TGCA_C
Cuts at: 3806 3806
Size: 5036
ApoI r'AATT_y
Cuts at: 1061 1486 2040 4389 4645 1061
Size: 425 554 2349 256 1452
Fragments arranged by size:
2349 1452 554 425 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5036
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
AvaI C'yCGr_G
Cuts at: 869 1137 2056 869
Size: 268 919 3849
Fragments arranged by size:
3849 919 268
BamHI G'GATC_C
Cuts at: 2061 2061
Size: 5036
BanI G'GyrC_C
Cuts at: 1255 1290 1728 2052 2466 2714 2749 1255
Size: 35 438 324 414 248 35 3542
Fragments arranged by size:
3542 438 414 324 248 35 35
BanII G_rGCy'C
Cuts at: 211 543 682 744 1144 1166 1174 1445 1651
Size: 332 139 62 400 22 8 271 206
Cuts at: 1651 1819 1948 2050 3080 211
Size: 168 129 102 1030 2167
Fragments arranged by size:
2167 1030 400 332 271 206 168 139
129 102 62 22 8
BbeI G_GCGC'C
Cuts at: 2470 2718 2470
Size: 248 4788
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3695 5036 2934
Size: 761 1341 2934
Fragments arranged by size:
2934 1341 761
BclI T'GATC_A
Cuts at: 2556 2556
Size: 5036
BglI GCCn_nnn'nGGC
Cuts at: 827 1750 827
Size: 923 4113
BglII A'GATC_T
Cuts at: 2551 4588 2551
Size: 2037 2999
BlpI GC'TnA_GC
Cuts at: 980 2148 980
Size: 1168 3868
Bme1580I G_kGCm'C
Cuts at: 211 543 724 744 1144 1174 1295 1623 1651
Size: 332 181 20 400 30 121 328 28
Cuts at: 1651 1948 2661 2754 3810 211
Size: 297 713 93 1056 1437
Fragments arranged by size:
1437 1056 713 400 332 328 297 181
121 93 30 28 20
BmrI ACTGGGnnnn_n'
Cuts at: 1701 1813 1951 2248 2329 2664 4808 1701
Size: 112 138 297 81 335 2144 1929
Fragments arranged by size:
2144 1929 335 297 138 112 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1101 3403 1101
Size: 2302 2734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 922 1319 1347 606
Size: 316 397 28 4295
Fragments arranged by size:
4295 397 316 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1587 2212 3091 3583 3881 493
Size: 1094 625 879 492 298 1648
Fragments arranged by size:
1648 1094 879 625 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4740 4740
Size: 5036
BsaAI yAC'GTr
Cuts at: 2389 2389
Size: 5036
BsaBI GATnn'nnATC
Cuts at: 6 2550 4587 6
Size: 2544 2037 455
Fragments arranged by size:
2544 2037 455
BsaHI Gr'CG_yC
Cuts at: 2467 2715 3417 4913 2467
Size: 248 702 1496 2590
Fragments arranged by size:
2590 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2225 2746 3424 3698 3845 4256 2225
Size: 521 678 274 147 411 3005
Fragments arranged by size:
3005 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 646
Size: 30 5006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 775 949 496
Size: 279 174 4583
Fragments arranged by size:
4583 279 174
BseYI C'CCAG_C
Cuts at: 386 725 747 839 1132 1369 1612 1784 2461
Size: 339 22 92 293 237 243 172 677
Cuts at: 2461 3219 3796 4292 386
Size: 758 577 496 1130
Fragments arranged by size:
1130 758 677 577 496 339 293 243
237 172 92 22
BsiEI CG_ry'CG
Cuts at: 104 2578 2624 3832 104
Size: 2474 46 1208 1308
Fragments arranged by size:
2474 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1445 1740 2050 2828 3018 3810 1445
Size: 295 310 778 190 792 2671
Fragments arranged by size:
2671 792 778 310 295 190
Bsp1286I G_dGCh'C
Cuts at: 211 543 682 724 744 1144 1166 1174 1295
Size: 332 139 42 20 400 22 8 121
Cuts at: 1295 1445 1623 1651 1740 1819 1948 2050 2661
Size: 150 178 28 89 79 129 102 611
Cuts at: 2661 2754 2828 3018 3080 3810 211
Size: 93 74 190 62 730 1437
Fragments arranged by size:
1437 730 611 400 332 190 178 150
139 129 121 102 93 89 79 74
62 42 28 22 20 8
BspEI T'CCGG_A
Cuts at: 4256 4256
Size: 5036
BspHI T'CATG_A
Cuts at: 275 4212 275
Size: 3937 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2086 2300 2602 2983 2086
Size: 214 302 381 4139
Fragments arranged by size:
4139 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3328 3382 4866 5031 50
Size: 3278 54 1484 165 55
Fragments arranged by size:
3278 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 2948 1958
Size: 990 4046
BsrFI r'CCGG_y
Cuts at: 3034 3215 3424 3034
Size: 181 209 4646
Fragments arranged by size:
4646 209 181
BsrGI T'GTAC_A
Cuts at: 329 329
Size: 5036
BssSI C'ACGA_G
Cuts at: 3307 3665 3307
Size: 358 4678
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 2413 1735
Size: 678 4358
BstBI TT'CG_AA
Cuts at: 3397 3397
Size: 5036
BstEII G'GTnAC_C
Cuts at: 1683 1683
Size: 5036
BstXI CCAn_nnnn'nTGG
Cuts at: 588 925 1552 1875 2005 588
Size: 337 627 323 130 3619
Fragments arranged by size:
3619 627 337 323 130
BstYI r'GATC_y
Cuts at: 1 2061 2528 2551 2886 3132 4144 4230 4242
Size: 2060 467 23 335 246 1012 86 12
Cuts at: 4242 4588 1
Size: 346 449
Fragments arranged by size:
2060 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1981 1981
Size: 5036
Bsu36I CC'TnA_GG
Cuts at: 707 997 707
Size: 290 4746
BtgI C'CryG_G
Cuts at: 202 306 696 1145 1282 1868 1892 202
Size: 104 390 449 137 586 24 3346
Fragments arranged by size:
3346 586 449 390 137 104 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2986 3167 3330 2986
Size: 181 163 4692
Fragments arranged by size:
4692 181 163
BtsI GCAGTG_nn'
Cuts at: 397 1623 1977 2279 397
Size: 1226 354 302 3154
Fragments arranged by size:
3154 1226 354 302
DraI TTT'AAA
Cuts at: 2035 2035
Size: 5036
DraIII CAC_nnn'GTG
Cuts at: 1735 1735
Size: 5036
DrdI GACnn_nn'nnGTC
Cuts at: 117 2742 3600 117
Size: 2625 858 1553
Fragments arranged by size:
2625 1553 858
EaeI y'GGCC_r
Cuts at: 1243 2621 2795 3186 3213 4558 1243
Size: 1378 174 391 27 1345 1721
Fragments arranged by size:
1721 1378 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2621 2621
Size: 5036
EarI CTCTTCn'nnn_
Cuts at: 926 1450 3059 3269 926
Size: 524 1609 210 2693
Fragments arranged by size:
2693 1609 524 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3244 3554 3700 4846 3244
Size: 310 146 1146 3434
Fragments arranged by size:
3434 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1443 2048 1443
Size: 605 4431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 606 922 925 1217 1319 1347 1431 2861
Size: 161 316 3 292 102 28 84 1430
Cuts at: 2861 3293 4040 4308 445
Size: 432 747 268 1173
Fragments arranged by size:
1430 1173 747 432 316 292 268 161
102 84 28 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1853 1853
Size: 5036
EcoO109I rG'GnC_Cy
Cuts at: 207 539 740 741 1141 1170 1433 1648 1945
Size: 332 201 1 400 29 263 215 297
Cuts at: 1945 2175 207
Size: 230 3068
Fragments arranged by size:
3068 400 332 297 263 230 215 201
29 1
EcoRI G'AATT_C
Cuts at: 2040 2040
Size: 5036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 955 3055 955
Size: 2100 2936
FspI TGC'GCA
Cuts at: 2817 2817
Size: 5036
HaeII r_GCGC'y
Cuts at: 340 786 2470 2718 3740 4719 340
Size: 446 1684 248 1022 979 657
Fragments arranged by size:
1684 1022 979 657 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2878 2908 2910 2940 2878
Size: 30 2 30 4974
Fragments arranged by size:
4974 30 30 2
HincII GTy'rAC
Cuts at: 1761 2075 1761
Size: 314 4722
HpaI GTT'AAC
Cuts at: 1761 1761
Size: 5036
KasI G'GCGC_C
Cuts at: 2466 2714 2466
Size: 248 4788
KpnI G_GTAC'C
Cuts at: 1259 2056 1259
Size: 797 4239
MluI A'CGCG_T
Cuts at: 1388 4469 1388
Size: 3081 1955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1091 3707 3891 1091
Size: 2616 184 2236
Fragments arranged by size:
2616 2236 184
MscI TGG'CCA
Cuts at: 1245 2797 1245
Size: 1552 3484
MslI CAynn'nnrTG
Cuts at: 280 472 586 1873 3025 3152 280
Size: 192 114 1287 1152 127 2164
Fragments arranged by size:
2164 1287 1152 192 127 114
MspA1I CmG'CkG
Cuts at: 747 945 951 1147 1216 1267 1791 1894 2147
Size: 198 6 196 69 51 524 103 253
Cuts at: 2147 2461 2821 3834 4079 747
Size: 314 360 1013 245 1704
Fragments arranged by size:
1704 1013 524 360 314 253 245 198
196 103 69 51 6
NaeI GCC'GGC
Cuts at: 3217 3217
Size: 5036
NarI GG'CG_CC
Cuts at: 2467 2715 2467
Size: 248 4788
NcoI C'CATG_G
Cuts at: 306 696 1282 1868 306
Size: 390 586 586 3474
Fragments arranged by size:
3474 586 586 390
NgoMIV G'CCGG_C
Cuts at: 3215 3215
Size: 5036
NspI r_CATG'y
Cuts at: 328 2089 2589 3496 328
Size: 1761 500 907 1868
Fragments arranged by size:
1868 1761 907 500
PasI CC'CwG_GG
Cuts at: 1166 1202 1337 1166
Size: 36 135 4865
Fragments arranged by size:
4865 135 36
PciI A'CATG_T
Cuts at: 3492 3492
Size: 5036
PflMI CCAn_nnn'nTGG
Cuts at: 1202 1252 1202
Size: 50 4986
PfoI T'CCnGG_A
Cuts at: 179 179
Size: 5036
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1660 1692 4175 4207 142
Size: 32 1486 32 2483 32 971
Fragments arranged by size:
2483 1486 971 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1433 1433
Size: 5036
PspOMI G'GGCC_C
Cuts at: 207 539 740 1140 1170 1647 1944 207
Size: 332 201 400 30 477 297 3299
Fragments arranged by size:
3299 477 400 332 297 201 30
PstI C_TGCA'G
Cuts at: 770 950 2083 2768 770
Size: 180 1133 685 3038
Fragments arranged by size:
3038 1133 685 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5036
PvuII CAG'CTG
Cuts at: 945 951 1267 2461 2821 945
Size: 6 316 1194 360 3160
Fragments arranged by size:
3160 1194 360 316 6
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5036
SacI G_AGCT'C
Cuts at: 1445 2050 1445
Size: 605 4431
SacII CC_GC'GG
Cuts at: 1148 1895 1148
Size: 747 4289
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5036
SapI GCTCTTCn'nnn_
Cuts at: 1450 3059 3269 1450
Size: 1609 210 3217
Fragments arranged by size:
3217 1609 210
SbfI CC_TGCA'GG
Cuts at: 2083 2083
Size: 5036
ScaI AGT'ACT
Cuts at: 1230 4553 1230
Size: 3323 1713
SexAI A'CCwGG_T
Cuts at: 1731 1731
Size: 5036
SfcI C'TryA_G
Cuts at: 33 766 946 2079 2764 3757 3948 33
Size: 733 180 1133 685 993 191 1121
Fragments arranged by size:
1133 1121 993 733 685 191 180
SfoI GGC'GCC
Cuts at: 2468 2716 2468
Size: 248 4788
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
SmaI CCC'GGG
Cuts at: 2058 2058
Size: 5036
SmlI C'TyrA_G
Cuts at: 508 1602 2191 3106 3598 3860 508
Size: 1094 589 915 492 262 1684
Fragments arranged by size:
1684 1094 915 589 492 262
SpeI A'CTAG_T
Cuts at: 67 922 67
Size: 855 4181
SphI G_CATG'C
Cuts at: 2089 2589 2089
Size: 500 4536
StuI AGG'CCT
Cuts at: 712 1692 712
Size: 980 4056
StyI C'CwwG_G
Cuts at: 306 696 1282 1868 2170 2524 306
Size: 390 586 586 302 354 2818
Fragments arranged by size:
2818 586 586 390 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 244 461 2619 2752 3211 3420 244
Size: 217 2158 133 459 209 1860
Fragments arranged by size:
2158 1860 459 217 209 133
TatI w'GTAC_w
Cuts at: 329 437 1228 4551 329
Size: 108 791 3323 814
Fragments arranged by size:
3323 814 791 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1301 1333 4553 4585 1301
Size: 32 3220 32 1752
Fragments arranged by size:
3220 1752 32 32
Tth111I GACn'n_nGTC
Cuts at: 117 2833 117
Size: 2716 2320
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 580 607 830 1659 2531 564
Size: 16 27 223 829 872 3069
Fragments arranged by size:
3069 872 829 223 27 16
XmaI C'CCGG_G
Cuts at: 2056 2056
Size: 5036
XmnI GAAnn'nnTTC
Cuts at: 406 430 406
Size: 24 5012
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BssHII ClaI CspCI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI