Restriction map for
pF1KA1041
(Circular) (Six-base) MAPSORT of: pF1KA1041.seq Check: 2309 from: 1 to: 4979
pF1KA1041 4979 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1541 2017 1541
Size: 476 4503
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 2804 3236 3589 4251 1775
Size: 1029 432 353 662 2503
Fragments arranged by size:
2503 1029 662 432 353
AflIII A'CryG_T
Cuts at: 2331 4131 4412 2331
Size: 1800 281 2898
Fragments arranged by size:
2898 1800 281
AgeI A'CCGG_T
Cuts at: 864 864
Size: 4979
AlwNI CAG_nnn'CTG
Cuts at: 2284 3722 2284
Size: 1438 3541
ApaLI G'TGCA_C
Cuts at: 3817 3817
Size: 4979
ApoI r'AATT_y
Cuts at: 263 416 477 1755 1983 4332 4588 263
Size: 153 61 1278 228 2349 256 654
Fragments arranged by size:
2349 1278 654 256 228 153 61
AseI AT'TA_AT
Cuts at: 20 380 20
Size: 360 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 1999 1999
Size: 4979
BamHI G'GATC_C
Cuts at: 2004 2004
Size: 4979
BanI G'GyrC_C
Cuts at: 1422 1711 1995 2409 2657 2692 1422
Size: 289 284 414 248 35 3709
Fragments arranged by size:
3709 414 289 284 248 35
BanII G_rGCy'C
Cuts at: 1993 3023 1993
Size: 1030 3949
BbeI G_GCGC'C
Cuts at: 2413 2661 2413
Size: 248 4731
BbvCI CC'TCA_GC
Cuts at: 908 908
Size: 4979
BciVI GTATCCnnnnn_n'
Cuts at: 1455 2877 3933 4979 1455
Size: 1422 1056 1046 1455
Fragments arranged by size:
1455 1422 1056 1046
BclI T'GATC_A
Cuts at: 756 2499 756
Size: 1743 3236
BfrBI ATG'CAT
Cuts at: 1719 1719
Size: 4979
BglI GCCn_nnn'nGGC
Cuts at: 603 603
Size: 4979
BglII A'GATC_T
Cuts at: 978 1587 2494 4531 978
Size: 609 907 2037 1426
Fragments arranged by size:
2037 1426 907 609
BlpI GC'TnA_GC
Cuts at: 2091 2091
Size: 4979
Bme1580I G_kGCm'C
Cuts at: 621 2604 2697 3821 621
Size: 1983 93 1124 1779
Fragments arranged by size:
1983 1779 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 570 2191 2272 2607 4751 570
Size: 1621 81 335 2144 798
Fragments arranged by size:
2144 1621 798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 4979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1321 1826 1916 187
Size: 1134 505 90 3250
Fragments arranged by size:
3250 1134 505 90
Bpu10I CC'TnA_GC
Cuts at: 908 908
Size: 4979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1576 2155 3034 3748 4046 1576
Size: 579 879 714 298 2509
Fragments arranged by size:
2509 879 714 579 298
BsaI GGTCTCn'nnnn_
Cuts at: 1215 4683 1215
Size: 3468 1511
BsaAI yAC'GTr
Cuts at: 2332 2332
Size: 4979
BsaBI GATnn'nnATC
Cuts at: 6 1051 2493 4530 6
Size: 1045 1442 2037 455
Fragments arranged by size:
2037 1442 1045 455
BsaHI Gr'CG_yC
Cuts at: 2410 2658 3360 4856 2410
Size: 248 702 1496 2533
Fragments arranged by size:
2533 1496 702 248
BsaWI w'CCGG_w
Cuts at: 864 2168 2689 3367 3778 3925 4199 864
Size: 1304 521 678 411 147 274 1644
Fragments arranged by size:
1644 1304 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 896 896
Size: 4979
BseYI C'CCAG_C
Cuts at: 1070 1108 1305 1352 2404 3162 3827 4235 1070
Size: 38 197 47 1052 758 665 408 1814
Fragments arranged by size:
1814 1052 758 665 408 197 47 38
BsiEI CG_ry'CG
Cuts at: 104 1267 2521 2567 3797 104
Size: 1163 1254 46 1230 1286
Fragments arranged by size:
1286 1254 1230 1163 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1993 2771 2961 3821 1126
Size: 867 778 190 860 2284
Fragments arranged by size:
2284 867 860 778 190
BsmI GAATG_Cn'
Cuts at: 282 282
Size: 4979
Bsp1286I G_dGCh'C
Cuts at: 621 1126 1993 2604 2697 2771 2961 3023 3821
Size: 505 867 611 93 74 190 62 798
Cuts at: 3821 621
Size: 1779
Fragments arranged by size:
1779 867 798 611 505 190 93 74
62
BspHI T'CATG_A
Cuts at: 3411 3411
Size: 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2029 2243 2545 2926 2029
Size: 214 302 381 4082
Fragments arranged by size:
4082 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3271 3325 4809 4974 50
Size: 3221 54 1484 165 55
Fragments arranged by size:
3221 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2891 2891
Size: 4979
BsrFI r'CCGG_y
Cuts at: 864 2977 3158 864
Size: 2113 181 2685
Fragments arranged by size:
2685 2113 181
BsrGI T'GTAC_A
Cuts at: 1760 1760
Size: 4979
BssSI C'ACGA_G
Cuts at: 3250 3958 3250
Size: 708 4271
BstAPI GCAn_nnn'nTGC
Cuts at: 1893 2356 1893
Size: 463 4516
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstEII G'GTnAC_C
Cuts at: 194 194
Size: 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 1744 1744
Size: 4979
BstYI r'GATC_y
Cuts at: 1 978 1587 2004 2471 2494 2829 3075 3381
Size: 977 609 417 467 23 335 246 306
Cuts at: 3381 3393 3479 4531 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 977 609 467 449 417 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4979
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2929 3110 3273 2929
Size: 181 163 4635
Fragments arranged by size:
4635 181 163
BtsI GCAGTG_nn'
Cuts at: 471 1176 1527 2222 471
Size: 705 351 695 3228
Fragments arranged by size:
3228 705 695 351
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 128 2685 4029 128
Size: 2557 1344 1078
Fragments arranged by size:
2557 1344 1078
EaeI y'GGCC_r
Cuts at: 604 2564 2738 3129 3156 4501 604
Size: 1960 174 391 27 1345 1082
Fragments arranged by size:
1960 1345 1082 391 174 27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4979
EarI CTCTTCn'nnn_
Cuts at: 607 3002 3212 607
Size: 2395 210 2374
Fragments arranged by size:
2395 2374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3187 3929 4075 4789 3187
Size: 742 146 714 3377
Fragments arranged by size:
3377 742 714 146
EcoICRI GAG'CTC
Cuts at: 1991 1991
Size: 4979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1321 1775 1826 1916 2804 3236 3589 4251
Size: 1134 454 51 90 888 432 353 662
Cuts at: 4251 187
Size: 915
Fragments arranged by size:
1134 915 888 662 454 432 353 90
51
EcoO109I rG'GnC_Cy
Cuts at: 554 2118 554
Size: 1564 3415
EcoRI G'AATT_C
Cuts at: 477 1983 477
Size: 1506 3473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 4979
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4979
HaeII r_GCGC'y
Cuts at: 2413 2661 3891 4662 2413
Size: 248 1230 771 2730
Fragments arranged by size:
2730 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 861 893 2821 2851 2853 2883 206
Size: 32 623 32 1928 30 2 30 2302
Fragments arranged by size:
2302 1928 623 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1036 1412 2018 1036
Size: 376 606 3997
Fragments arranged by size:
3997 606 376
HpaI GTT'AAC
Cuts at: 1412 1412
Size: 4979
KasI G'GCGC_C
Cuts at: 2409 2657 2409
Size: 248 4731
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MfeI C'AATT_G
Cuts at: 1967 1967
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 451 730 822 1181 1747 3738 3922 345
Size: 106 279 92 359 566 1991 184 1402
Fragments arranged by size:
1991 1402 566 359 279 184 106 92
MscI TGG'CCA
Cuts at: 2740 2740
Size: 4979
MslI CAynn'nnrTG
Cuts at: 332 1169 2968 3095 332
Size: 837 1799 127 2216
Fragments arranged by size:
2216 1799 837 127
MspA1I CmG'CkG
Cuts at: 2090 2404 2764 3548 3793 2090
Size: 314 360 784 245 3276
Fragments arranged by size:
3276 784 360 314 245
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 4979
NarI GG'CG_CC
Cuts at: 2410 2658 2410
Size: 248 4731
NcoI C'CATG_G
Cuts at: 107 107
Size: 4979
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 4979
NsiI A_TGCA'T
Cuts at: 1721 1721
Size: 4979
NspI r_CATG'y
Cuts at: 2032 2532 4135 2032
Size: 500 1603 2876
Fragments arranged by size:
2876 1603 500
PciI A'CATG_T
Cuts at: 4131 4131
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 816 1807 816
Size: 991 3988
PfoI T'CCnGG_A
Cuts at: 1842 1943 1842
Size: 101 4878
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4947
PpuMI rG'GwC_Cy
Cuts at: 554 554
Size: 4979
PsiI TTA'TAA
Cuts at: 1007 1007
Size: 4979
PstI C_TGCA'G
Cuts at: 2026 2711 2026
Size: 685 4294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 2404 2764 2404
Size: 360 4619
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1993 1993
Size: 4979
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SapI GCTCTTCn'nnn_
Cuts at: 3002 3212 3002
Size: 210 4769
SbfI CC_TGCA'GG
Cuts at: 2026 2026
Size: 4979
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SexAI A'CCwGG_T
Cuts at: 1610 1610
Size: 4979
SfcI C'TryA_G
Cuts at: 33 626 2022 2707 3675 3866 33
Size: 593 1396 685 968 191 1146
Fragments arranged by size:
1396 1146 968 685 593 191
SfoI GGC'GCC
Cuts at: 2411 2659 2411
Size: 248 4731
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SgrAI Cr'CCGG_yG
Cuts at: 864 864
Size: 4979
SmaI CCC'GGG
Cuts at: 2001 2001
Size: 4979
SmlI C'TyrA_G
Cuts at: 1591 2134 3049 3763 4025 1591
Size: 543 915 714 262 2545
Fragments arranged by size:
2545 915 714 543 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4479
StyI C'CwwG_G
Cuts at: 107 2113 2467 107
Size: 2006 354 2619
Fragments arranged by size:
2619 2006 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 405 1145 1250 1319 1331 2562 2695 3154 3363
Size: 740 105 69 12 1231 133 459 209
Cuts at: 3363 405
Size: 2021
Fragments arranged by size:
2021 1231 740 459 209 133 105 69
12
TatI w'GTAC_w
Cuts at: 923 1114 1760 4494 923
Size: 191 646 2734 1408
Fragments arranged by size:
2734 1408 646 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 4947
Tth111I GACn'n_nGTC
Cuts at: 2776 2776
Size: 4979
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1267 2474 1267
Size: 1207 3772
XmaI C'CCGG_G
Cuts at: 1999 1999
Size: 4979
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BspEI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI