Restriction map for
pF1KA1033
(Circular) (Six-base) MAPSORT of: pF1KA1033.seq Check: 2959 from: 1 to: 6632
pF1KA1033 6632 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 220 3092 3670 220
Size: 2872 578 3182
Fragments arranged by size:
3182 2872 578
Acc65I G'GTAC_C
Cuts at: 1146 3648 1146
Size: 2502 4130
AclI AA'CG_TT
Cuts at: 6472 6472
Size: 6632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1418 3370 3492 3551 4457 4889 5636 5904
Size: 544 1952 122 59 906 432 747 268
Cuts at: 5904 874
Size: 1602
Fragments arranged by size:
1952 1602 906 747 544 432 268 122
59
AflII C'TTAA_G
Cuts at: 190 190
Size: 6632
AflIII A'CryG_T
Cuts at: 1490 2003 3984 5088 6065 1490
Size: 513 1981 1104 977 2057
Fragments arranged by size:
2057 1981 1104 977 513
AgeI A'CCGG_T
Cuts at: 5020 5020
Size: 6632
AhdI GACnn_n'nnGTC
Cuts at: 3448 3448
Size: 6632
AlwNI CAG_nnn'CTG
Cuts at: 546 1219 3937 5504 546
Size: 673 2718 1567 1674
Fragments arranged by size:
2718 1674 1567 673
ApaLI G'TGCA_C
Cuts at: 5402 5402
Size: 6632
ApoI r'AATT_y
Cuts at: 170 205 439 509 805 812 967 1058 1390
Size: 35 234 70 296 7 155 91 332
Cuts at: 1390 2105 2779 2961 3054 3109 3141 3463 3500
Size: 715 674 182 93 55 32 322 37
Cuts at: 3500 3636 5985 6241 170
Size: 136 2349 256 561
Fragments arranged by size:
2349 715 674 561 332 322 296 256
234 182 155 136 93 91 70 55
37 35 32 7
AseI AT'TA_AT
Cuts at: 20 2561 20
Size: 2541 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6632
AvaI C'yCGr_G
Cuts at: 3652 3652
Size: 6632
BamHI G'GATC_C
Cuts at: 3657 3657
Size: 6632
BanI G'GyrC_C
Cuts at: 1146 3648 4062 4310 4345 1146
Size: 2502 414 248 35 3433
Fragments arranged by size:
3433 2502 414 248 35
BanII G_rGCy'C
Cuts at: 1339 3646 4676 1339
Size: 2307 1030 3295
Fragments arranged by size:
3295 2307 1030
BbeI G_GCGC'C
Cuts at: 4066 4314 4066
Size: 248 6384
BbsI GAAGACnn'nnnn_
Cuts at: 1237 1935 2899 1237
Size: 698 964 4970
Fragments arranged by size:
4970 964 698
BbvCI CC'TCA_GC
Cuts at: 962 962
Size: 6632
BciVI GTATCCnnnnn_n'
Cuts at: 1263 2768 4530 5291 6632 1263
Size: 1505 1762 761 1341 1263
Fragments arranged by size:
1762 1505 1341 1263 761
BclI T'GATC_A
Cuts at: 2748 2835 3042 4152 2748
Size: 87 207 1110 5228
Fragments arranged by size:
5228 1110 207 87
BfrBI ATG'CAT
Cuts at: 1447 2043 1447
Size: 596 6036
BglII A'GATC_T
Cuts at: 1401 2154 2896 4147 6184 1401
Size: 753 742 1251 2037 1849
Fragments arranged by size:
2037 1849 1251 753 742
BlpI GC'TnA_GC
Cuts at: 3371 3744 3371
Size: 373 6259
Bme1580I G_kGCm'C
Cuts at: 4257 4350 5406 4257
Size: 93 1056 5483
Fragments arranged by size:
5483 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 144 2358 2417 3844 3925 4260 6404 144
Size: 2214 59 1427 81 335 2144 372
Fragments arranged by size:
2214 2144 1427 372 335 81 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 240 4999 240
Size: 4759 1873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 3089 674
Size: 2415 4217
Bpu10I CC'TnA_GC
Cuts at: 962 962
Size: 6632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1540 3808 4687 5179 5477 232
Size: 1308 2268 879 492 298 1387
Fragments arranged by size:
2268 1387 1308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 457 3578 6336 457
Size: 3121 2758 753
Fragments arranged by size:
3121 2758 753
BsaAI yAC'GTr
Cuts at: 2292 3985 2292
Size: 1693 4939
BsaBI GATnn'nnATC
Cuts at: 6 284 3296 3452 4146 6183 6
Size: 278 3012 156 694 2037 455
Fragments arranged by size:
3012 2037 694 455 278 156
BsaHI Gr'CG_yC
Cuts at: 4063 4311 5013 6509 4063
Size: 248 702 1496 4186
Fragments arranged by size:
4186 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3821 4342 5020 5294 5441 5852 3821
Size: 521 678 274 147 411 4601
Fragments arranged by size:
4601 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 923 953 109
Size: 30 784 30 5788
Fragments arranged by size:
5788 784 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 944 229
Size: 715 5917
BseYI C'CCAG_C
Cuts at: 4057 4815 5392 5888 4057
Size: 758 577 496 4801
Fragments arranged by size:
4801 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1557 377
Size: 1180 5452
BsiEI CG_ry'CG
Cuts at: 104 4174 4220 5428 104
Size: 4070 46 1208 1308
Fragments arranged by size:
4070 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 683 1339 1718 3646 4424 4614 5406 683
Size: 656 379 1928 778 190 792 1909
Fragments arranged by size:
1928 1909 792 778 656 379 190
BsmI GAATG_Cn'
Cuts at: 3186 3186
Size: 6632
Bsp1286I G_dGCh'C
Cuts at: 683 1339 1718 3646 4257 4350 4424 4614 4676
Size: 656 379 1928 611 93 74 190 62
Cuts at: 4676 5406 683
Size: 730 1909
Fragments arranged by size:
1928 1909 730 656 611 379 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5852 5852
Size: 6632
BspHI T'CATG_A
Cuts at: 1856 2343 5808 1856
Size: 487 3465 2680
Fragments arranged by size:
3465 2680 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2040 3682 3896 4198 4579 2040
Size: 1642 214 302 381 4093
Fragments arranged by size:
4093 1642 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4924 4978 6462 6627 50
Size: 4874 54 1484 165 55
Fragments arranged by size:
4874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2928 3585 4544 2928
Size: 657 959 5016
Fragments arranged by size:
5016 959 657
BsrFI r'CCGG_y
Cuts at: 4630 4811 5020 4630
Size: 181 209 6242
Fragments arranged by size:
6242 209 181
BsrGI T'GTAC_A
Cuts at: 764 1311 3384 764
Size: 547 2073 4012
Fragments arranged by size:
4012 2073 547
BssSI C'ACGA_G
Cuts at: 4903 5261 4903
Size: 358 6274
BstAPI GCAn_nnn'nTGC
Cuts at: 4009 4009
Size: 6632
BstBI TT'CG_AA
Cuts at: 1095 2589 3114 4993 1095
Size: 1494 525 1879 2734
Fragments arranged by size:
2734 1879 1494 525
BstXI CCAn_nnnn'nTGG
Cuts at: 114 492 3267 114
Size: 378 2775 3479
Fragments arranged by size:
3479 2775 378
BstYI r'GATC_y
Cuts at: 1 1401 1869 2154 2896 3657 4124 4147 4482
Size: 1400 468 285 742 761 467 23 335
Cuts at: 4482 4728 5740 5826 5838 6184 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1400 1012 761 742 468 467 449 346
335 285 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 221 221
Size: 6632
Bsu36I CC'TnA_GG
Cuts at: 2816 2816
Size: 6632
BtgI C'CryG_G
Cuts at: 107 3260 107
Size: 3153 3479
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4582 4763 4926 4582
Size: 181 163 6288
Fragments arranged by size:
6288 181 163
BtsI GCAGTG_nn'
Cuts at: 3498 3875 3498
Size: 377 6255
DraI TTT'AAA
Cuts at: 1824 2111 2183 3631 1824
Size: 287 72 1448 4825
Fragments arranged by size:
4825 1448 287 72
DraIII CAC_nnn'GTG
Cuts at: 1798 1909 1798
Size: 111 6521
DrdI GACnn_nn'nnGTC
Cuts at: 4338 5196 4338
Size: 858 5774
EaeI y'GGCC_r
Cuts at: 1661 4217 4391 4782 4809 6154 1661
Size: 2556 174 391 27 1345 2139
Fragments arranged by size:
2556 2139 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4217 4217
Size: 6632
EarI CTCTTCn'nnn_
Cuts at: 1649 4655 4865 1649
Size: 3006 210 3416
Fragments arranged by size:
3416 3006 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4840 5150 5296 6442 4840
Size: 310 146 1146 5030
Fragments arranged by size:
5030 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1337 3644 1337
Size: 2307 4325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 874 1418 3089 3370 3492 3551 4457 4889
Size: 200 544 1671 281 122 59 906 432
Cuts at: 4889 5636 5904 674
Size: 747 268 1402
Fragments arranged by size:
1671 1402 906 747 544 432 281 268
200 122 59
EcoO109I rG'GnC_Cy
Cuts at: 2442 3771 2442
Size: 1329 5303
EcoRI G'AATT_C
Cuts at: 812 3636 812
Size: 2824 3808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 205 1030 3007 4651 205
Size: 825 1977 1644 2186
Fragments arranged by size:
2186 1977 1644 825
FspI TGC'GCA
Cuts at: 4413 4413
Size: 6632
HaeII r_GCGC'y
Cuts at: 4066 4314 5336 6315 4066
Size: 248 1022 979 4383
Fragments arranged by size:
4383 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 145 177 1300 1332 1702 1734 1880
Size: 32 4 32 1123 32 370 32 146
Cuts at: 1880 1912 3434 3466 4474 4504 4506 4536 109
Size: 32 1522 32 1008 30 2 30 2205
Fragments arranged by size:
2205 1522 1123 1008 370 146 32 32
32 32 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 153 3671 153
Size: 3518 3114
HindIII A'AGCT_T
Cuts at: 307 3283 307
Size: 2976 3656
KasI G'GCGC_C
Cuts at: 4062 4310 4062
Size: 248 6384
KpnI G_GTAC'C
Cuts at: 1150 3652 1150
Size: 2502 4130
MfeI C'AATT_G
Cuts at: 263 263
Size: 6632
MluI A'CGCG_T
Cuts at: 6065 6065
Size: 6632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 210 1035 5303 5487 210
Size: 825 4268 184 1355
Fragments arranged by size:
4268 1355 825 184
MscI TGG'CCA
Cuts at: 1663 4393 1663
Size: 2730 3902
MslI CAynn'nnrTG
Cuts at: 112 1495 1594 1810 3265 4621 4748 112
Size: 1383 99 216 1455 1356 127 1996
Fragments arranged by size:
1996 1455 1383 1356 216 127 99
MspA1I CmG'CkG
Cuts at: 1219 3743 4057 4417 5430 5675 1219
Size: 2524 314 360 1013 245 2176
Fragments arranged by size:
2524 2176 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4813 4813
Size: 6632
NarI GG'CG_CC
Cuts at: 4063 4311 4063
Size: 248 6384
NcoI C'CATG_G
Cuts at: 107 3260 107
Size: 3153 3479
NdeI CA'TA_TG
Cuts at: 1571 1571
Size: 6632
NgoMIV G'CCGG_C
Cuts at: 4811 4811
Size: 6632
NsiI A_TGCA'T
Cuts at: 1449 2045 1449
Size: 596 6036
NspI r_CATG'y
Cuts at: 1494 2007 2047 3685 4185 5092 1494
Size: 513 40 1638 500 907 3034
Fragments arranged by size:
3034 1638 907 513 500 40
PciI A'CATG_T
Cuts at: 1490 2003 5088 1490
Size: 513 3085 3034
Fragments arranged by size:
3085 3034 513
PflMI CCAn_nnn'nTGG
Cuts at: 600 600
Size: 6632
PmeI GTTT'AAAC
Cuts at: 3631 3631
Size: 6632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1558 1590 5771 5803 1558
Size: 32 4181 32 2387
Fragments arranged by size:
4181 2387 32 32
PsiI TTA'TAA
Cuts at: 1118 1118
Size: 6632
PstI C_TGCA'G
Cuts at: 3679 4364 3679
Size: 685 5947
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6632
PvuII CAG'CTG
Cuts at: 1219 4057 4417 1219
Size: 2838 360 3434
Fragments arranged by size:
3434 2838 360
RsrII CG'GwC_CG
Cuts at: 4827 4827
Size: 6632
SacI G_AGCT'C
Cuts at: 1339 3646 1339
Size: 2307 4325
SalI G'TCGA_C
Cuts at: 3669 3669
Size: 6632
SapI GCTCTTCn'nnn_
Cuts at: 1649 4655 4865 1649
Size: 3006 210 3416
Fragments arranged by size:
3416 3006 210
SbfI CC_TGCA'GG
Cuts at: 3679 3679
Size: 6632
ScaI AGT'ACT
Cuts at: 954 6149 954
Size: 5195 1437
SfcI C'TryA_G
Cuts at: 33 296 476 1578 2333 3457 3675 4360 5353
Size: 263 180 1102 755 1124 218 685 993
Cuts at: 5353 5544 33
Size: 191 1121
Fragments arranged by size:
1124 1121 1102 993 755 685 263 218
191 180
SfoI GGC'GCC
Cuts at: 4064 4312 4064
Size: 248 6384
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6632
SmaI CCC'GGG
Cuts at: 3654 3654
Size: 6632
SmlI C'TyrA_G
Cuts at: 190 211 1555 3787 4702 5194 5456 190
Size: 21 1344 2232 915 492 262 1366
Fragments arranged by size:
2232 1366 1344 915 492 262 21
SnaBI TAC'GTA
Cuts at: 2292 2292
Size: 6632
SpeI A'CTAG_T
Cuts at: 67 2973 67
Size: 2906 3726
SphI G_CATG'C
Cuts at: 2047 3685 4185 2047
Size: 1638 500 4494
Fragments arranged by size:
4494 1638 500
SspI AAT'ATT
Cuts at: 1173 2565 3076 1173
Size: 1392 511 4729
Fragments arranged by size:
4729 1392 511
StuI AGG'CCT
Cuts at: 895 895
Size: 6632
StyI C'CwwG_G
Cuts at: 107 3260 3766 4120 107
Size: 3153 506 354 2619
Fragments arranged by size:
3153 2619 506 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 648 974 2210 3253 4215 4348 4807 5016 648
Size: 326 1236 1043 962 133 459 209 2264
Fragments arranged by size:
2264 1236 1043 962 459 326 209 133
TatI w'GTAC_w
Cuts at: 764 952 1311 3384 6147 764
Size: 188 359 2073 2763 1249
Fragments arranged by size:
2763 2073 1249 359 188
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 923 955 2680 2712 6149 6181 923
Size: 32 1725 32 3437 32 1374
Fragments arranged by size:
3437 1725 1374 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 123 4429 123
Size: 4306 2326
XbaI T'CTAG_A
Cuts at: 3663 3663
Size: 6632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2430 4127 2430
Size: 1697 4935
XmaI C'CCGG_G
Cuts at: 3652 3652
Size: 6632
XmnI GAAnn'nnTTC
Cuts at: 1838 1838
Size: 6632
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstEII ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI ZraI