Restriction map for
pF1KA1018
(Circular) (Six-base) MAPSORT of: pF1KA1018.seq Check: 2077 from: 1 to: 6164
pF1KA1018 6164 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 231 231
Size: 6164
AccI GT'mk_AC
Cuts at: 937 3202 937
Size: 2265 3899
Acc65I G'GTAC_C
Cuts at: 3180 3180
Size: 6164
AclI AA'CG_TT
Cuts at: 6004 6004
Size: 6164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 926 1019 1526 2604 2667 2866 3125 3989
Size: 246 93 507 1078 63 199 259 864
Cuts at: 3989 4421 5168 5436 680
Size: 432 747 268 1408
Fragments arranged by size:
1408 1078 864 747 507 432 268 259
246 199 93 63
AflIII A'CryG_T
Cuts at: 749 2150 2442 3516 4620 5597 749
Size: 1401 292 1074 1104 977 1316
Fragments arranged by size:
1401 1316 1104 1074 977 292
AgeI A'CCGG_T
Cuts at: 1216 4552 1216
Size: 3336 2828
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2966 2966
Size: 6164
AlwNI CAG_nnn'CTG
Cuts at: 1345 1486 1593 3096 3469 5036 1345
Size: 141 107 1503 373 1567 2473
Fragments arranged by size:
2473 1567 1503 373 141 107
ApaI G_GGCC'C
Cuts at: 2920 2920
Size: 6164
ApaLI G'TGCA_C
Cuts at: 2718 4934 2718
Size: 2216 3948
ApoI r'AATT_y
Cuts at: 348 429 631 719 1324 2198 3168 5517 5773
Size: 81 202 88 605 874 970 2349 256
Cuts at: 5773 348
Size: 739
Fragments arranged by size:
2349 970 874 739 605 256 202 88
81
AseI AT'TA_AT
Cuts at: 20 20
Size: 6164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
AvaI C'yCGr_G
Cuts at: 842 2788 3184 842
Size: 1946 396 3822
Fragments arranged by size:
3822 1946 396
BamHI G'GATC_C
Cuts at: 324 2310 3189 324
Size: 1986 879 3299
Fragments arranged by size:
3299 1986 879
BanI G'GyrC_C
Cuts at: 258 281 2942 3180 3594 3842 3877 258
Size: 23 2661 238 414 248 35 2545
Fragments arranged by size:
2661 2545 414 248 238 35 23
BanII G_rGCy'C
Cuts at: 1997 2920 3178 4208 1997
Size: 923 258 1030 3953
Fragments arranged by size:
3953 1030 923 258
BbeI G_GCGC'C
Cuts at: 3598 3846 3598
Size: 248 5916
BbsI GAAGACnn'nnnn_
Cuts at: 953 1796 2673 953
Size: 843 877 4444
Fragments arranged by size:
4444 877 843
BciVI GTATCCnnnnn_n'
Cuts at: 4062 4823 6164 4062
Size: 761 1341 4062
Fragments arranged by size:
4062 1341 761
BclI T'GATC_A
Cuts at: 3684 3684
Size: 6164
BglI GCCn_nnn'nGGC
Cuts at: 2515 2698 2758 2812 2915 2515
Size: 183 60 54 103 5764
Fragments arranged by size:
5764 183 103 60 54
BglII A'GATC_T
Cuts at: 3679 5716 3679
Size: 2037 4127
BlpI GC'TnA_GC
Cuts at: 3276 3276
Size: 6164
Bme1580I G_kGCm'C
Cuts at: 2470 2722 2920 3789 3882 4938 2470
Size: 252 198 869 93 1056 3696
Fragments arranged by size:
3696 1056 869 252 198 93
BmgBI CAC'GTC
Cuts at: 2876 2876
Size: 6164
BmrI ACTGGGnnnn_n'
Cuts at: 1986 3376 3457 3792 5936 1986
Size: 1390 81 335 2144 2214
Fragments arranged by size:
2214 2144 1390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 4531 2995
Size: 1536 4628
Bpu10I CC'TnA_GC
Cuts at: 1988 2891 3154 1988
Size: 903 263 4998
Fragments arranged by size:
4998 903 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2776 3340 4219 4711 5009 1288
Size: 1488 564 879 492 298 2443
Fragments arranged by size:
2443 1488 879 564 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5868 5868
Size: 6164
BsaAI yAC'GTr
Cuts at: 597 2445 2822 3517 597
Size: 1848 377 695 3244
Fragments arranged by size:
3244 1848 695 377
BsaBI GATnn'nnATC
Cuts at: 6 3678 5715 6
Size: 3672 2037 455
Fragments arranged by size:
3672 2037 455
BsaHI Gr'CG_yC
Cuts at: 1601 1793 2745 3595 3843 4545 6041 1601
Size: 192 952 850 248 702 1496 1724
Fragments arranged by size:
1724 1496 952 850 702 248 192
BsaWI w'CCGG_w
Cuts at: 1216 2313 2673 3353 3874 4552 4826 4973 5384
Size: 1097 360 680 521 678 274 147 411
Cuts at: 5384 1216
Size: 1996
Fragments arranged by size:
1996 1097 680 678 521 411 360 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1171 1201 1171
Size: 30 6134
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1115 2566 2629 2806 1115
Size: 1451 63 177 4473
Fragments arranged by size:
4473 1451 177 63
BseYI C'CCAG_C
Cuts at: 2748 2861 3096 3589 4347 4924 5420 2748
Size: 113 235 493 758 577 496 3492
Fragments arranged by size:
3492 758 577 496 493 235 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 1688 2958 1620
Size: 68 1270 4826
Fragments arranged by size:
4826 1270 68
BsiEI CG_ry'CG
Cuts at: 104 1848 3706 3752 4960 104
Size: 1744 1858 46 1208 1308
Fragments arranged by size:
1858 1744 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1997 2545 2722 2927 3178 3956 4146 4938 1997
Size: 548 177 205 251 778 190 792 3223
Fragments arranged by size:
3223 792 778 548 251 205 190 177
BsmI GAATG_Cn'
Cuts at: 782 1169 1467 2701 782
Size: 387 298 1234 4245
Fragments arranged by size:
4245 1234 387 298
BsmBI CGTCTCn'nnnn_
Cuts at: 2882 2882
Size: 6164
Bsp1286I G_dGCh'C
Cuts at: 1997 2470 2545 2722 2920 2927 3178 3789 3882
Size: 473 75 177 198 7 251 611 93
Cuts at: 3882 3956 4146 4208 4938 1997
Size: 74 190 62 730 3223
Fragments arranged by size:
3223 730 611 473 251 198 190 177
93 75 74 62 7
BspEI T'CCGG_A
Cuts at: 2313 2673 5384 2313
Size: 360 2711 3093
Fragments arranged by size:
3093 2711 360
BspHI T'CATG_A
Cuts at: 2778 5340 2778
Size: 2562 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 324 3214 3428 3730 4111 231
Size: 93 2890 214 302 381 2284
Fragments arranged by size:
2890 2284 381 302 214 93
BsrBI CCG'CTC
Cuts at: 50 4456 4510 5994 6159 50
Size: 4406 54 1484 165 55
Fragments arranged by size:
4406 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1203 1967 4076 1203
Size: 764 2109 3291
Fragments arranged by size:
3291 2109 764
BsrFI r'CCGG_y
Cuts at: 278 637 1216 4162 4343 4552 278
Size: 359 579 2946 181 209 1890
Fragments arranged by size:
2946 1890 579 359 209 181
BssSI C'ACGA_G
Cuts at: 4435 4793 4435
Size: 358 5806
BstAPI GCAn_nnn'nTGC
Cuts at: 223 1093 2465 3541 223
Size: 870 1372 1076 2846
Fragments arranged by size:
2846 1372 1076 870
BstBI TT'CG_AA
Cuts at: 4525 4525
Size: 6164
BstXI CCAn_nnnn'nTGG
Cuts at: 1695 1695
Size: 6164
BstYI r'GATC_y
Cuts at: 1 324 2310 2895 3189 3656 3679 4014 4260
Size: 323 1986 585 294 467 23 335 246
Cuts at: 4260 5272 5358 5370 5716 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1986 1012 585 467 449 346 335 323
294 246 86 23 12
BtgI C'CryG_G
Cuts at: 2531 2531
Size: 6164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4114 4295 4458 4114
Size: 181 163 5820
Fragments arranged by size:
5820 181 163
BtsI GCAGTG_nn'
Cuts at: 1676 3407 1676
Size: 1731 4433
DraI TTT'AAA
Cuts at: 495 756 1679 3163 495
Size: 261 923 1484 3496
Fragments arranged by size:
3496 1484 923 261
DraIII CAC_nnn'GTG
Cuts at: 2476 2476
Size: 6164
DrdI GACnn_nn'nnGTC
Cuts at: 3870 4728 3870
Size: 858 5306
EaeI y'GGCC_r
Cuts at: 441 1616 1845 1964 3749 3923 4314 4341 5686
Size: 1175 229 119 1785 174 391 27 1345
Cuts at: 5686 441
Size: 919
Fragments arranged by size:
1785 1345 1175 919 391 229 174 119
27
EagI C'GGCC_G
Cuts at: 1845 3749 1845
Size: 1904 4260
EarI CTCTTCn'nnn_
Cuts at: 699 770 1412 2077 2407 2885 4187 4397 699
Size: 71 642 665 330 478 1302 210 2466
Fragments arranged by size:
2466 1302 665 642 478 330 210 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 4372 4682 4828 5974 2323
Size: 2049 310 146 1146 2513
Fragments arranged by size:
2513 2049 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1995 3176 1995
Size: 1181 4983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 926 1019 1526 2604 2667 2866 3125 3989
Size: 246 93 507 1078 63 199 259 864
Cuts at: 3989 4421 5168 5436 680
Size: 432 747 268 1408
Fragments arranged by size:
1408 1078 864 747 507 432 268 259
246 199 93 63
EcoO109I rG'GnC_Cy
Cuts at: 1726 2916 2917 2976 3040 3303 1726
Size: 1190 1 59 64 263 4587
Fragments arranged by size:
4587 1190 263 64 59 1
EcoRI G'AATT_C
Cuts at: 3168 3168
Size: 6164
EcoRV GAT'ATC
Cuts at: 1158 1158
Size: 6164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 970 2418 4183 970
Size: 1448 1765 2951
Fragments arranged by size:
2951 1765 1448
FspI TGC'GCA
Cuts at: 3945 3945
Size: 6164
HaeII r_GCGC'y
Cuts at: 2271 3598 3846 4868 5847 2271
Size: 1327 248 1022 979 2588
Fragments arranged by size:
2588 1327 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1057 1089 1171 1203 2103 2135 2887 2919 4006
Size: 32 82 32 900 32 752 32 1087
Cuts at: 4006 4036 4038 4068 1057
Size: 30 2 30 3153
Fragments arranged by size:
3153 1087 900 752 82 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1769 1839 3203 1769
Size: 70 1364 4730
Fragments arranged by size:
4730 1364 70
HindIII A'AGCT_T
Cuts at: 153 153
Size: 6164
KasI G'GCGC_C
Cuts at: 3594 3842 3594
Size: 248 5916
KpnI G_GTAC'C
Cuts at: 3184 3184
Size: 6164
MluI A'CGCG_T
Cuts at: 5597 5597
Size: 6164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3114 4835 5019 3114
Size: 1721 184 4259
Fragments arranged by size:
4259 1721 184
MscI TGG'CCA
Cuts at: 443 1618 1966 3925 443
Size: 1175 348 1959 2682
Fragments arranged by size:
2682 1959 1175 348
MslI CAynn'nnrTG
Cuts at: 287 1936 2153 2659 4153 4280 287
Size: 1649 217 506 1494 127 2171
Fragments arranged by size:
2171 1649 1494 506 217 127
MspA1I CmG'CkG
Cuts at: 1053 1593 2521 2582 2772 2861 3275 3589 3949
Size: 540 928 61 190 89 414 314 360
Cuts at: 3949 4962 5207 1053
Size: 1013 245 2010
Fragments arranged by size:
2010 1013 928 540 414 360 314 245
190 89 61
NaeI GCC'GGC
Cuts at: 4345 4345
Size: 6164
NarI GG'CG_CC
Cuts at: 3595 3843 3595
Size: 248 5916
NdeI CA'TA_TG
Cuts at: 794 794
Size: 6164
NgoMIV G'CCGG_C
Cuts at: 4343 4343
Size: 6164
NspI r_CATG'y
Cuts at: 1004 1941 2154 3217 3717 4624 1004
Size: 937 213 1063 500 907 2544
Fragments arranged by size:
2544 1063 937 907 500 213
PasI CC'CwG_GG
Cuts at: 1730 1730
Size: 6164
PciI A'CATG_T
Cuts at: 2150 4620 2150
Size: 2470 3694
PflMI CCAn_nnn'nTGG
Cuts at: 2476 2476
Size: 6164
PmeI GTTT'AAAC
Cuts at: 3163 3163
Size: 6164
PmlI CAC'GTG
Cuts at: 2445 2822 2445
Size: 377 5787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 5303
Size: 32 6132
PspOMI G'GGCC_C
Cuts at: 2916 2916
Size: 6164
PstI C_TGCA'G
Cuts at: 1089 1133 2141 2771 3092 3211 3896 1089
Size: 44 1008 630 321 119 685 3357
Fragments arranged by size:
3357 1008 685 630 321 119 44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6164
PvuII CAG'CTG
Cuts at: 1053 1593 2772 2861 3589 3949 1053
Size: 540 1179 89 728 360 3268
Fragments arranged by size:
3268 1179 728 540 360 89
RsrII CG'GwC_CG
Cuts at: 4359 4359
Size: 6164
SacI G_AGCT'C
Cuts at: 1997 3178 1997
Size: 1181 4983
SalI G'TCGA_C
Cuts at: 3201 3201
Size: 6164
SapI GCTCTTCn'nnn_
Cuts at: 4187 4397 4187
Size: 210 5954
SbfI CC_TGCA'GG
Cuts at: 3211 3211
Size: 6164
ScaI AGT'ACT
Cuts at: 5681 5681
Size: 6164
SexAI A'CCwGG_T
Cuts at: 2987 2987
Size: 6164
SfcI C'TryA_G
Cuts at: 33 1085 1129 1474 2137 2767 3088 3207 3892
Size: 1052 44 345 663 630 321 119 685
Cuts at: 3892 4885 5076 33
Size: 993 191 1121
Fragments arranged by size:
1121 1052 993 685 663 630 345 321
191 119 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 2515 2515
Size: 6164
SfoI GGC'GCC
Cuts at: 3596 3844 3596
Size: 248 5916
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
SmaI CCC'GGG
Cuts at: 844 3186 844
Size: 2342 3822
SmlI C'TyrA_G
Cuts at: 1267 2755 3319 4234 4726 4988 1267
Size: 1488 564 915 492 262 2443
Fragments arranged by size:
2443 1488 915 564 492 262
SnaBI TAC'GTA
Cuts at: 597 597
Size: 6164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6164
SphI G_CATG'C
Cuts at: 3217 3717 3217
Size: 500 5664
StuI AGG'CCT
Cuts at: 144 144
Size: 6164
StyI C'CwwG_G
Cuts at: 1694 3298 3652 1694
Size: 1604 354 4206
Fragments arranged by size:
4206 1604 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1786 2084 2799 3747 3880 4339 4548 1786
Size: 298 715 948 133 459 209 3402
Fragments arranged by size:
3402 948 715 459 298 209 133
TatI w'GTAC_w
Cuts at: 5679 5679
Size: 6164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5681 5713 5681
Size: 32 6132
Tth111I GACn'n_nGTC
Cuts at: 2966 3961 2966
Size: 995 5169
XbaI T'CTAG_A
Cuts at: 3195 3195
Size: 6164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 3659 2599
Size: 1060 5104
XmaI C'CCGG_G
Cuts at: 842 3184 842
Size: 2342 3822
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BpmI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PfoI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SgrAI SrfI SspI SwaI XhoI XmnI ZraI