Restriction map for
pF1KA1009
(Circular) (Six-base) MAPSORT of: pF1KA1009.seq Check: 2620 from: 1 to: 7094
pF1KA1009 7094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 3062 3062
Size: 7094
AccI GT'mk_AC
Cuts at: 1618 2330 4132 1618
Size: 712 1802 4580
Fragments arranged by size:
4580 1802 712
Acc65I G'GTAC_C
Cuts at: 1335 4110 1335
Size: 2775 4319
AclI AA'CG_TT
Cuts at: 6934 6934
Size: 7094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1193 1325 1460 2704 3196 3478 3491 3740
Size: 130 132 135 1244 492 282 13 249
Cuts at: 3740 4919 5351 5704 6366 1063
Size: 1179 432 353 662 1791
Fragments arranged by size:
1791 1244 1179 662 492 432 353 282
249 135 132 130 13
AflII C'TTAA_G
Cuts at: 148 171 148
Size: 23 7071
AflIII A'CryG_T
Cuts at: 1080 1194 4446 6246 6527 1080
Size: 114 3252 1800 281 1647
Fragments arranged by size:
3252 1800 1647 281 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2930 2930
Size: 7094
AlwNI CAG_nnn'CTG
Cuts at: 1651 2649 2737 4399 5837 1651
Size: 998 88 1662 1438 2908
Fragments arranged by size:
2908 1662 1438 998 88
ApaLI G'TGCA_C
Cuts at: 488 1358 5932 488
Size: 870 4574 1650
Fragments arranged by size:
4574 1650 870
ApoI r'AATT_y
Cuts at: 531 876 1021 2226 2414 2639 2767 3103 3600
Size: 345 145 1205 188 225 128 336 497
Cuts at: 3600 3734 4098 6447 6703 531
Size: 134 364 2349 256 922
Fragments arranged by size:
2349 1205 922 497 364 345 336 256
225 188 145 134 128
AseI AT'TA_AT
Cuts at: 20 383 1274 3436 20
Size: 363 891 2162 3678
Fragments arranged by size:
3678 2162 891 363
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7094
AvaI C'yCGr_G
Cuts at: 4114 4114
Size: 7094
AvrII C'CTAG_G
Cuts at: 1531 1531
Size: 7094
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1680 1713 1680
Size: 33 7061
BamHI G'GATC_C
Cuts at: 4119 4119
Size: 7094
BanI G'GyrC_C
Cuts at: 674 1335 4110 4524 4772 4807 674
Size: 661 2775 414 248 35 2961
Fragments arranged by size:
2961 2775 661 414 248 35
BanII G_rGCy'C
Cuts at: 218 844 1417 2213 4108 5138 218
Size: 626 573 796 1895 1030 2174
Fragments arranged by size:
2174 1895 1030 796 626 573
BbeI G_GCGC'C
Cuts at: 4528 4776 4528
Size: 248 6846
BbsI GAAGACnn'nnnn_
Cuts at: 803 2294 803
Size: 1491 5603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2158 2192 2158
Size: 34 7060
BciVI GTATCCnnnnn_n'
Cuts at: 1681 4992 6048 7094 1681
Size: 3311 1056 1046 1681
Fragments arranged by size:
3311 1681 1056 1046
BclI T'GATC_A
Cuts at: 4614 4614
Size: 7094
BglI GCCn_nnn'nGGC
Cuts at: 3280 3280
Size: 7094
BglII A'GATC_T
Cuts at: 1288 2076 2235 4609 6646 1288
Size: 788 159 2374 2037 1736
Fragments arranged by size:
2374 2037 1736 788 159
BlpI GC'TnA_GC
Cuts at: 4206 4206
Size: 7094
Bme1580I G_kGCm'C
Cuts at: 492 560 1362 4719 4812 5936 492
Size: 68 802 3357 93 1124 1650
Fragments arranged by size:
3357 1650 1124 802 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 1427 4306 4387 4722 6866 1427
Size: 2879 81 335 2144 1655
Fragments arranged by size:
2879 2144 1655 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5461 5461
Size: 7094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1997 2671 2827 3997 1997
Size: 674 156 1170 5094
Fragments arranged by size:
5094 1170 674 156
Bpu10I CC'TnA_GC
Cuts at: 665 1416 665
Size: 751 6343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 845 1052 1429 1524 2174 2572 3288 3629
Size: 532 207 377 95 650 398 716 341
Cuts at: 3629 4270 5149 5863 6161 313
Size: 641 879 714 298 1246
Fragments arranged by size:
1246 879 716 714 650 641 532 398
377 341 298 207 95
BsaI GGTCTCn'nnnn_
Cuts at: 6798 6798
Size: 7094
BsaAI yAC'GTr
Cuts at: 4447 4447
Size: 7094
BsaBI GATnn'nnATC
Cuts at: 6 4608 6645 6
Size: 4602 2037 455
Fragments arranged by size:
4602 2037 455
BsaHI Gr'CG_yC
Cuts at: 3059 4525 4773 5475 6971 3059
Size: 1466 248 702 1496 3182
Fragments arranged by size:
3182 1496 1466 702 248
BsaWI w'CCGG_w
Cuts at: 4283 4804 5482 5893 6040 6314 4283
Size: 521 678 411 147 274 5063
Fragments arranged by size:
5063 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2098 2128 4031 4061 2098
Size: 30 1903 30 5131
Fragments arranged by size:
5131 1903 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 280 937 2119 136
Size: 144 657 1182 5111
Fragments arranged by size:
5111 1182 657 144
BseYI C'CCAG_C
Cuts at: 4519 5277 5942 6350 4519
Size: 758 665 408 5263
Fragments arranged by size:
5263 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4012
Size: 7094
BsiEI CG_ry'CG
Cuts at: 104 4636 4682 5912 104
Size: 4532 46 1230 1286
Fragments arranged by size:
4532 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 218 492 844 1362 1417 4108 4886 5076 5936
Size: 274 352 518 55 2691 778 190 860
Cuts at: 5936 218
Size: 1376
Fragments arranged by size:
2691 1376 860 778 518 352 274 190
55
BsmI GAATG_Cn'
Cuts at: 3051 4080 3051
Size: 1029 6065
Bsp1286I G_dGCh'C
Cuts at: 218 492 560 844 1362 1417 2213 4108 4719
Size: 274 68 284 518 55 796 1895 611
Cuts at: 4719 4812 4886 5076 5138 5936 218
Size: 93 74 190 62 798 1376
Fragments arranged by size:
1895 1376 798 796 611 518 284 274
190 93 74 68 62 55
BspHI T'CATG_A
Cuts at: 5526 5526
Size: 7094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2718 4144 4358 4660 5041 2718
Size: 1426 214 302 381 4771
Fragments arranged by size:
4771 1426 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5386 5440 6924 7089 50
Size: 5336 54 1484 165 55
Fragments arranged by size:
5336 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3533 5006 3533
Size: 1473 5621
BsrFI r'CCGG_y
Cuts at: 4031 5092 5273 4031
Size: 1061 181 5852
Fragments arranged by size:
5852 1061 181
BssSI C'ACGA_G
Cuts at: 5365 6073 5365
Size: 708 6386
BstAPI GCAn_nnn'nTGC
Cuts at: 2629 3535 4471 2629
Size: 906 936 5252
Fragments arranged by size:
5252 936 906
BstBI TT'CG_AA
Cuts at: 2336 5455 2336
Size: 3119 3975
BstYI r'GATC_y
Cuts at: 1 1288 2076 2235 2646 4119 4586 4609 4944
Size: 1287 788 159 411 1473 467 23 335
Cuts at: 4944 5190 5496 5508 5594 6646 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1473 1287 1052 788 467 449 411 335
306 246 159 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1683 1683
Size: 7094
BtgI C'CryG_G
Cuts at: 107 2846 107
Size: 2739 4355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5044 5225 5388 5044
Size: 181 163 6750
Fragments arranged by size:
6750 181 163
BtsI GCAGTG_nn'
Cuts at: 162 3482 4337 162
Size: 3320 855 2919
Fragments arranged by size:
3320 2919 855
ClaI AT'CG_AT
Cuts at: 1510 1510
Size: 7094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3182 3217 3182
Size: 35 7059
DraI TTT'AAA
Cuts at: 369 1728 4093 369
Size: 1359 2365 3370
Fragments arranged by size:
3370 2365 1359
DrdI GACnn_nn'nnGTC
Cuts at: 4800 6144 4800
Size: 1344 5750
EaeI y'GGCC_r
Cuts at: 1001 4679 4853 5244 5271 6616 1001
Size: 3678 174 391 27 1345 1479
Fragments arranged by size:
3678 1479 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4679 4679
Size: 7094
EarI CTCTTCn'nnn_
Cuts at: 113 506 866 1870 2680 5117 5327 113
Size: 393 360 1004 810 2437 210 1880
Fragments arranged by size:
2437 1880 1004 810 393 360 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5302 6044 6190 6904 5302
Size: 742 146 714 5492
Fragments arranged by size:
5492 742 714 146
EcoICRI GAG'CTC
Cuts at: 216 842 1415 4106 216
Size: 626 573 2691 3204
Fragments arranged by size:
3204 2691 626 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1193 1325 1460 1997 2671 2704 2827 3196
Size: 130 132 135 537 674 33 123 369
Cuts at: 3196 3478 3491 3740 3997 4919 5351 5704 6366
Size: 282 13 249 257 922 432 353 662
Cuts at: 6366 1063
Size: 1791
Fragments arranged by size:
1791 922 674 662 537 432 369 353
282 257 249 135 132 130 123 33
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2962 2962
Size: 7094
EcoO109I rG'GnC_Cy
Cuts at: 4233 4233
Size: 7094
EcoRI G'AATT_C
Cuts at: 531 876 3103 4098 531
Size: 345 2227 995 3527
Fragments arranged by size:
3527 2227 995 345
EcoRV GAT'ATC
Cuts at: 834 2038 2695 834
Size: 1204 657 5233
Fragments arranged by size:
5233 1204 657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2524 2625 3670 5113 2041
Size: 483 101 1045 1443 4022
Fragments arranged by size:
4022 1443 1045 483 101
FspI TGC'GCA
Cuts at: 1766 4875 1766
Size: 3109 3985
HaeII r_GCGC'y
Cuts at: 4528 4776 6006 6777 4528
Size: 248 1230 771 4845
Fragments arranged by size:
4845 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 412 444 2679 2711 3079 3111 3253 3285 4936
Size: 32 2235 32 368 32 142 32 1651
Cuts at: 4936 4966 4968 4998 412
Size: 30 2 30 2508
Fragments arranged by size:
2508 2235 1651 368 142 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 497 4133 497
Size: 3636 3458
HindIII A'AGCT_T
Cuts at: 371 761 371
Size: 390 6704
KasI G'GCGC_C
Cuts at: 4524 4772 4524
Size: 248 6846
KpnI G_GTAC'C
Cuts at: 1339 4114 1339
Size: 2775 4319
MluI A'CGCG_T
Cuts at: 6527 6527
Size: 7094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1811 5853 6037 1811
Size: 4042 184 2868
Fragments arranged by size:
4042 2868 184
MscI TGG'CCA
Cuts at: 4855 4855
Size: 7094
MslI CAynn'nnrTG
Cuts at: 422 3044 5083 5210 422
Size: 2622 2039 127 2306
Fragments arranged by size:
2622 2306 2039 127
MspA1I CmG'CkG
Cuts at: 1157 1963 2737 4205 4519 4879 5663 5908 1157
Size: 806 774 1468 314 360 784 245 2343
Fragments arranged by size:
2343 1468 806 784 774 360 314 245
NaeI GCC'GGC
Cuts at: 5275 5275
Size: 7094
NarI GG'CG_CC
Cuts at: 4525 4773 4525
Size: 248 6846
NcoI C'CATG_G
Cuts at: 107 2846 107
Size: 2739 4355
NgoMIV G'CCGG_C
Cuts at: 5273 5273
Size: 7094
NspI r_CATG'y
Cuts at: 588 1084 1198 3859 4147 4647 6250 588
Size: 496 114 2661 288 500 1603 1432
Fragments arranged by size:
2661 1603 1432 500 496 288 114
PciI A'CATG_T
Cuts at: 1080 1194 6246 1080
Size: 114 5052 1928
Fragments arranged by size:
5052 1928 114
PflMI CCAn_nnn'nTGG
Cuts at: 786 786
Size: 7094
PmeI GTTT'AAAC
Cuts at: 4093 4093
Size: 7094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5540 5572 5540
Size: 32 7062
PsiI TTA'TAA
Cuts at: 1489 3658 1489
Size: 2169 4925
PstI C_TGCA'G
Cuts at: 1578 3528 3692 4141 4826 1578
Size: 1950 164 449 685 3846
Fragments arranged by size:
3846 1950 685 449 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7094
PvuII CAG'CTG
Cuts at: 1157 1963 2737 4519 4879 1157
Size: 806 774 1782 360 3372
Fragments arranged by size:
3372 1782 806 774 360
RsrII CG'GwC_CG
Cuts at: 5289 5289
Size: 7094
SacI G_AGCT'C
Cuts at: 218 844 1417 4108 218
Size: 626 573 2691 3204
Fragments arranged by size:
3204 2691 626 573
SalI G'TCGA_C
Cuts at: 4131 4131
Size: 7094
SapI GCTCTTCn'nnn_
Cuts at: 5117 5327 5117
Size: 210 6884
SbfI CC_TGCA'GG
Cuts at: 4141 4141
Size: 7094
ScaI AGT'ACT
Cuts at: 177 6611 177
Size: 6434 660
SfcI C'TryA_G
Cuts at: 33 1574 3098 3408 3524 3688 3739 4137 4822
Size: 1541 1524 310 116 164 51 398 685
Cuts at: 4822 5790 5981 33
Size: 968 191 1146
Fragments arranged by size:
1541 1524 1146 968 685 398 310 191
164 116 51
SfoI GGC'GCC
Cuts at: 4526 4774 4526
Size: 248 6846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7094
SmaI CCC'GGG
Cuts at: 4116 4116
Size: 7094
SmlI C'TyrA_G
Cuts at: 148 171 292 860 1067 1444 1503 2189 2551
Size: 23 121 568 207 377 59 686 362
Cuts at: 2551 3303 3644 4249 5164 5878 6140 148
Size: 752 341 605 915 714 262 1102
Fragments arranged by size:
1102 915 752 714 686 605 568 377
362 341 262 207 121 59 23
SpeI A'CTAG_T
Cuts at: 67 201 67
Size: 134 6960
SphI G_CATG'C
Cuts at: 588 4147 4647 588
Size: 3559 500 3035
Fragments arranged by size:
3559 3035 500
SspI AAT'ATT
Cuts at: 1059 1218 2720 4007 1059
Size: 159 1502 1287 4146
Fragments arranged by size:
4146 1502 1287 159
StyI C'CwwG_G
Cuts at: 107 670 1531 2846 4228 4582 107
Size: 563 861 1315 1382 354 2619
Fragments arranged by size:
2619 1382 1315 861 563 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4677 4810 5269 5478 4677
Size: 133 459 209 6293
Fragments arranged by size:
6293 459 209 133
TatI w'GTAC_w
Cuts at: 175 6609 175
Size: 6434 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3523 3555 4031 4063 6611 6643 3523
Size: 32 476 32 2548 32 3974
Fragments arranged by size:
3974 2548 476 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4891 4891
Size: 7094
XbaI T'CTAG_A
Cuts at: 4125 4125
Size: 7094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4589 4589
Size: 7094
XmaI C'CCGG_G
Cuts at: 4114 4114
Size: 7094
XmnI GAAnn'nnTTC
Cuts at: 137 948 2678 3089 3734 3811 137
Size: 811 1730 411 645 77 3420
Fragments arranged by size:
3420 1730 811 645 411 77
ZraI GAC'GTC
Cuts at: 3060 3060
Size: 7094
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AleI AloI ApaI AscI
BbvCI BfrBI BmgBI BmtI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI