Restriction map for
pF1KA1006
(Circular) (Six-base) MAPSORT of: pF1KA1006.seq Check: 6389 from: 1 to: 6671
pF1KA1006 6671 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2953 3709 2953
Size: 756 5915
Acc65I G'GTAC_C
Cuts at: 3656 3687 3656
Size: 31 6640
AclI AA'CG_TT
Cuts at: 6511 6511
Size: 6671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 4496 4928 5281 5943 1768
Size: 2728 432 353 662 2496
Fragments arranged by size:
2728 2496 662 432 353
AfeI AGC'GCT
Cuts at: 2335 2335
Size: 6671
AflIII A'CryG_T
Cuts at: 1624 4023 5823 6104 1624
Size: 2399 1800 281 2191
Fragments arranged by size:
2399 2191 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1563 3083 3976 5414 1563
Size: 1520 893 1438 2820
Fragments arranged by size:
2820 1520 1438 893
ApaLI G'TGCA_C
Cuts at: 3521 5509 3521
Size: 1988 4683
ApoI r'AATT_y
Cuts at: 254 562 1183 1219 1528 1732 2538 3248 3675
Size: 308 621 36 309 204 806 710 427
Cuts at: 3675 6024 6280 254
Size: 2349 256 645
Fragments arranged by size:
2349 806 710 645 621 427 309 308
256 204 36
AseI AT'TA_AT
Cuts at: 20 2830 20
Size: 2810 3861
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6671
AvaI C'yCGr_G
Cuts at: 397 3691 397
Size: 3294 3377
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 791 824 791
Size: 33 6638
BamHI G'GATC_C
Cuts at: 2270 2859 3696 2270
Size: 589 837 5245
Fragments arranged by size:
5245 837 589
BanI G'GyrC_C
Cuts at: 478 3656 3687 4101 4349 4384 478
Size: 3178 31 414 248 35 2765
Fragments arranged by size:
3178 2765 414 248 35 31
BanII G_rGCy'C
Cuts at: 3404 3685 4715 3404
Size: 281 1030 5360
Fragments arranged by size:
5360 1030 281
BbeI G_GCGC'C
Cuts at: 4105 4353 4105
Size: 248 6423
BbsI GAAGACnn'nnnn_
Cuts at: 1122 1649 1951 2122 2466 2979 3130 1122
Size: 527 302 171 344 513 151 4663
Fragments arranged by size:
4663 527 513 344 302 171 151
BciVI GTATCCnnnnn_n'
Cuts at: 1420 4569 5625 6671 1420
Size: 3149 1056 1046 1420
Fragments arranged by size:
3149 1420 1056 1046
BclI T'GATC_A
Cuts at: 467 4191 467
Size: 3724 2947
BfrBI ATG'CAT
Cuts at: 1137 1137
Size: 6671
BglI GCCn_nnn'nGGC
Cuts at: 3088 3088
Size: 6671
BglII A'GATC_T
Cuts at: 1260 1842 4186 6223 1260
Size: 582 2344 2037 1708
Fragments arranged by size:
2344 2037 1708 582
BlpI GC'TnA_GC
Cuts at: 2052 3783 2052
Size: 1731 4940
Bme1580I G_kGCm'C
Cuts at: 3525 4296 4389 5513 3525
Size: 771 93 1124 4683
Fragments arranged by size:
4683 1124 771 93
BmrI ACTGGGnnnn_n'
Cuts at: 2475 3883 3964 4299 6443 2475
Size: 1408 81 335 2144 2703
Fragments arranged by size:
2703 2144 1408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3418 5038 3370
Size: 48 1620 5003
Fragments arranged by size:
5003 1620 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1952 2556 3515 1273
Size: 679 604 959 4429
Fragments arranged by size:
4429 959 679 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 3340 3847 4726 5440 5738 2350
Size: 990 507 879 714 298 3283
Fragments arranged by size:
3283 990 879 714 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 396 2192 6375 396
Size: 1796 4183 692
Fragments arranged by size:
4183 1796 692
BsaAI yAC'GTr
Cuts at: 4024 4024
Size: 6671
BsaBI GATnn'nnATC
Cuts at: 6 881 4185 6222 6
Size: 875 3304 2037 455
Fragments arranged by size:
3304 2037 875 455
BsaHI Gr'CG_yC
Cuts at: 4102 4350 5052 6548 4102
Size: 248 702 1496 4225
Fragments arranged by size:
4225 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1374 3860 4381 5059 5470 5617 5891 1374
Size: 2486 521 678 411 147 274 2154
Fragments arranged by size:
2486 2154 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 393 423 1815 1845 2640 2670 393
Size: 30 1392 30 795 30 4394
Fragments arranged by size:
4394 1392 795 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 1472 2180 144
Size: 1328 708 4635
Fragments arranged by size:
4635 1328 708
BseYI C'CCAG_C
Cuts at: 1853 2447 4096 4854 5519 5927 1853
Size: 594 1649 758 665 408 2597
Fragments arranged by size:
2597 1649 758 665 594 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 927 2358 3617 461
Size: 466 1431 1259 3515
Fragments arranged by size:
3515 1431 1259 466
BsiEI CG_ry'CG
Cuts at: 104 4213 4259 5489 104
Size: 4109 46 1230 1286
Fragments arranged by size:
4109 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1479 1888 3404 3525 3685 4463 4653 5513 1479
Size: 409 1516 121 160 778 190 860 2637
Fragments arranged by size:
2637 1516 860 778 409 190 160 121
BsmI GAATG_Cn'
Cuts at: 1967 1967
Size: 6671
BsmBI CGTCTCn'nnnn_
Cuts at: 3240 3240
Size: 6671
Bsp1286I G_dGCh'C
Cuts at: 1479 1888 3404 3525 3685 4296 4389 4463 4653
Size: 409 1516 121 160 611 93 74 190
Cuts at: 4653 4715 5513 1479
Size: 62 798 2637
Fragments arranged by size:
2637 1516 798 611 409 190 160 121
93 74 62
BspEI T'CCGG_A
Cuts at: 1374 1374
Size: 6671
BspHI T'CATG_A
Cuts at: 885 1329 3116 5103 885
Size: 444 1787 1987 2453
Fragments arranged by size:
2453 1987 1787 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 3721 3935 4237 4618 1060
Size: 2661 214 302 381 3113
Fragments arranged by size:
3113 2661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4963 5017 6501 6666 50
Size: 4913 54 1484 165 55
Fragments arranged by size:
4913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2091 2210 4583 2091
Size: 119 2373 4179
Fragments arranged by size:
4179 2373 119
BsrFI r'CCGG_y
Cuts at: 4669 4850 4669
Size: 181 6490
BsrGI T'GTAC_A
Cuts at: 741 1565 741
Size: 824 5847
BssSI C'ACGA_G
Cuts at: 4942 5650 4942
Size: 708 5963
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 3083 3529 4048 1360
Size: 1723 446 519 3983
Fragments arranged by size:
3983 1723 519 446
BstBI TT'CG_AA
Cuts at: 2472 5032 2472
Size: 2560 4111
BstXI CCAn_nnnn'nTGG
Cuts at: 919 2365 919
Size: 1446 5225
BstYI r'GATC_y
Cuts at: 1 1260 1842 2270 2859 3696 4163 4186 4521
Size: 1259 582 428 589 837 467 23 335
Cuts at: 4521 4767 5073 5085 5171 6223 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1259 1052 837 589 582 467 449 428
335 306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2954 2954
Size: 6671
BtgI C'CryG_G
Cuts at: 1319 2182 1319
Size: 863 5808
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4621 4802 4965 4621
Size: 181 163 6327
Fragments arranged by size:
6327 181 163
BtsI GCAGTG_nn'
Cuts at: 171 183 2370 2992 3084 3914 171
Size: 12 2187 622 92 830 2928
Fragments arranged by size:
2928 2187 830 622 92 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1984 2019 3044 3079 1984
Size: 35 1025 35 5576
Fragments arranged by size:
5576 1025 35 35
DraI TTT'AAA
Cuts at: 3670 3670
Size: 6671
DrdI GACnn_nn'nnGTC
Cuts at: 4377 5721 4377
Size: 1344 5327
EaeI y'GGCC_r
Cuts at: 1444 1951 4256 4430 4821 4848 6193 1444
Size: 507 2305 174 391 27 1345 1922
Fragments arranged by size:
2305 1922 1345 507 391 174 27
EagI C'GGCC_G
Cuts at: 4256 4256
Size: 6671
EarI CTCTTCn'nnn_
Cuts at: 3392 3563 4694 4904 3392
Size: 171 1131 210 5159
Fragments arranged by size:
5159 1131 210 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4879 5621 5767 6481 4879
Size: 742 146 714 5069
Fragments arranged by size:
5069 742 714 146
EcoICRI GAG'CTC
Cuts at: 3402 3683 3402
Size: 281 6390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1768 1952 2556 3515 4496 4928 5281 5943
Size: 495 184 604 959 981 432 353 662
Cuts at: 5943 1273
Size: 2001
Fragments arranged by size:
2001 981 959 662 604 495 432 353
184
EcoNI CCTnn'n_nnAGG
Cuts at: 3461 3461
Size: 6671
EcoO109I rG'GnC_Cy
Cuts at: 2452 3810 2452
Size: 1358 5313
EcoRI G'AATT_C
Cuts at: 3675 3675
Size: 6671
EcoRV GAT'ATC
Cuts at: 1975 1975
Size: 6671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 977 2002 2402 4690 977
Size: 1025 400 2288 2958
Fragments arranged by size:
2958 2288 1025 400
FspI TGC'GCA
Cuts at: 3446 4452 3446
Size: 1006 5665
HaeII r_GCGC'y
Cuts at: 2337 3491 4105 4353 5583 6354 2337
Size: 1154 614 248 1230 771 2654
Fragments arranged by size:
2654 1230 1154 771 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 671 703 808 840 2022 2054 4513 4543 4545
Size: 32 105 32 1182 32 2459 30 2
Cuts at: 4545 4575 671
Size: 30 2767
Fragments arranged by size:
2767 2459 1182 105 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3260 3710 3260
Size: 450 6221
HindIII A'AGCT_T
Cuts at: 1106 3413 1106
Size: 2307 4364
HpaI GTT'AAC
Cuts at: 3260 3260
Size: 6671
KasI G'GCGC_C
Cuts at: 4101 4349 4101
Size: 248 6423
KpnI G_GTAC'C
Cuts at: 3660 3691 3660
Size: 31 6640
MfeI C'AATT_G
Cuts at: 704 872 1996 704
Size: 168 1124 5379
Fragments arranged by size:
5379 1124 168
MluI A'CGCG_T
Cuts at: 6104 6104
Size: 6671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 603 2407 2652 3131 5430 5614 603
Size: 1804 245 479 2299 184 1660
Fragments arranged by size:
2299 1804 1660 479 245 184
MscI TGG'CCA
Cuts at: 1446 4432 1446
Size: 2986 3685
MslI CAynn'nnrTG
Cuts at: 434 917 1514 1660 2363 3121 3620 4660 4787
Size: 483 597 146 703 758 499 1040 127
Cuts at: 4787 434
Size: 2318
Fragments arranged by size:
2318 1040 758 703 597 499 483 146
127
MspA1I CmG'CkG
Cuts at: 445 3255 3782 4096 4456 5240 5485 445
Size: 2810 527 314 360 784 245 1631
Fragments arranged by size:
2810 1631 784 527 360 314 245
NaeI GCC'GGC
Cuts at: 4852 4852
Size: 6671
NarI GG'CG_CC
Cuts at: 4102 4350 4102
Size: 248 6423
NcoI C'CATG_G
Cuts at: 1319 1319
Size: 6671
NdeI CA'TA_TG
Cuts at: 1716 2058 2844 1716
Size: 342 786 5543
Fragments arranged by size:
5543 786 342
NgoMIV G'CCGG_C
Cuts at: 4850 4850
Size: 6671
NsiI A_TGCA'T
Cuts at: 1139 1139
Size: 6671
NspI r_CATG'y
Cuts at: 1349 1628 2232 3095 3300 3619 3724 4224 5827
Size: 279 604 863 205 319 105 500 1603
Cuts at: 5827 1349
Size: 2193
Fragments arranged by size:
2193 1603 863 604 500 319 279 205
105
PciI A'CATG_T
Cuts at: 1624 5823 1624
Size: 4199 2472
PflMI CCAn_nnn'nTGG
Cuts at: 1453 1453
Size: 6671
PfoI T'CCnGG_A
Cuts at: 1968 2454 3456 1968
Size: 486 1002 5183
Fragments arranged by size:
5183 1002 486
PmeI GTTT'AAAC
Cuts at: 3670 3670
Size: 6671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2385 2417 5117 5149 2385
Size: 32 2700 32 3907
Fragments arranged by size:
3907 2700 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2452
Size: 6671
PsiI TTA'TAA
Cuts at: 1730 1730
Size: 6671
PstI C_TGCA'G
Cuts at: 237 1559 3582 3718 4403 237
Size: 1322 2023 136 685 2505
Fragments arranged by size:
2505 2023 1322 685 136
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6671
PvuII CAG'CTG
Cuts at: 445 4096 4456 445
Size: 3651 360 2660
Fragments arranged by size:
3651 2660 360
RsrII CG'GwC_CG
Cuts at: 4866 4866
Size: 6671
SacI G_AGCT'C
Cuts at: 3404 3685 3404
Size: 281 6390
SalI G'TCGA_C
Cuts at: 3708 3708
Size: 6671
SapI GCTCTTCn'nnn_
Cuts at: 3392 4694 4904 3392
Size: 1302 210 5159
Fragments arranged by size:
5159 1302 210
SbfI CC_TGCA'GG
Cuts at: 3718 3718
Size: 6671
ScaI AGT'ACT
Cuts at: 1157 1391 2123 6188 1157
Size: 234 732 4065 1640
Fragments arranged by size:
4065 1640 732 234
SexAI A'CCwGG_T
Cuts at: 405 3462 405
Size: 3057 3614
SfcI C'TryA_G
Cuts at: 33 233 451 803 1400 1555 3289 3578 3714
Size: 200 218 352 597 155 1734 289 136
Cuts at: 3714 4399 5367 5558 33
Size: 685 968 191 1146
Fragments arranged by size:
1734 1146 968 685 597 352 289 218
200 191 155 136
SfoI GGC'GCC
Cuts at: 4103 4351 4103
Size: 248 6423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6671
SmaI CCC'GGG
Cuts at: 3693 3693
Size: 6671
SmlI C'TyrA_G
Cuts at: 2329 3355 3826 4741 5455 5717 2329
Size: 1026 471 915 714 262 3283
Fragments arranged by size:
3283 1026 915 714 471 262
SpeI A'CTAG_T
Cuts at: 67 2035 67
Size: 1968 4703
SphI G_CATG'C
Cuts at: 2232 3095 3724 4224 2232
Size: 863 629 500 4679
Fragments arranged by size:
4679 863 629 500
SspI AAT'ATT
Cuts at: 657 1079 1691 657
Size: 422 612 5637
Fragments arranged by size:
5637 612 422
StyI C'CwwG_G
Cuts at: 482 1319 1470 3805 4159 482
Size: 837 151 2335 354 2994
Fragments arranged by size:
2994 2335 837 354 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2812 2831 4254 4387 4846 5055 2812
Size: 19 1423 133 459 209 4428
Fragments arranged by size:
4428 1423 459 209 133 19
TatI w'GTAC_w
Cuts at: 741 1155 1389 1565 2121 6186 741
Size: 414 234 176 556 4065 1226
Fragments arranged by size:
4065 1226 556 414 234 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 393 425 6188 6220 393
Size: 32 5763 32 844
Fragments arranged by size:
5763 844 32 32
Tth111I GACn'n_nGTC
Cuts at: 4468 4468
Size: 6671
XbaI T'CTAG_A
Cuts at: 2554 3702 2554
Size: 1148 5523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 433 2685 2849 4166 433
Size: 2252 164 1317 2938
Fragments arranged by size:
2938 2252 1317 164
XmaI C'CCGG_G
Cuts at: 3691 3691
Size: 6671
XmnI GAAnn'nnTTC
Cuts at: 210 210
Size: 6671
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BmgBI BmtI Bpu10I
BsiWI BssHII BstEII Bsu36I ClaI DraIII FseI FspAI
NheI NotI NruI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI