Restriction map for
pF1KA1002
(Circular) (Six-base) MAPSORT of: pF1KA1002.seq Check: 2244 from: 1 to: 5633
pF1KA1002 5633 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1674 1674
Size: 5633
AccI GT'mk_AC
Cuts at: 2671 2671
Size: 5633
Acc65I G'GTAC_C
Cuts at: 1638 2649 1638
Size: 1011 4622
AclI AA'CG_TT
Cuts at: 5473 5473
Size: 5633
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 1079 2239 3458 3890 4243 4905 732
Size: 347 1160 1219 432 353 662 1460
Fragments arranged by size:
1460 1219 1160 662 432 353 347
AflIII A'CryG_T
Cuts at: 2985 4785 5066 2985
Size: 1800 281 3552
Fragments arranged by size:
3552 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 727 727
Size: 5633
AleI CACnn'nnGTG
Cuts at: 2494 2494
Size: 5633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 959 959
Size: 5633
AlwNI CAG_nnn'CTG
Cuts at: 697 1347 1452 2042 2415 2938 4376 697
Size: 650 105 590 373 523 1438 1954
Fragments arranged by size:
1954 1438 650 590 523 373 105
ApaLI G'TGCA_C
Cuts at: 2197 4471 2197
Size: 2274 3359
ApoI r'AATT_y
Cuts at: 166 241 448 2637 4986 5242 166
Size: 75 207 2189 2349 256 557
Fragments arranged by size:
2349 2189 557 256 207 75
AseI AT'TA_AT
Cuts at: 20 248 20
Size: 228 5405
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5633
AvaI C'yCGr_G
Cuts at: 770 2653 770
Size: 1883 3750
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1630 1663 1630
Size: 33 5600
BamHI G'GATC_C
Cuts at: 2603 2658 2603
Size: 55 5578
BanI G'GyrC_C
Cuts at: 841 1638 2380 2649 3063 3311 3346 841
Size: 797 742 269 414 248 35 3128
Fragments arranged by size:
3128 797 742 414 269 248 35
BanII G_rGCy'C
Cuts at: 1690 2647 3677 1690
Size: 957 1030 3646
Fragments arranged by size:
3646 1030 957
BbeI G_GCGC'C
Cuts at: 2384 3067 3315 2384
Size: 683 248 4702
Fragments arranged by size:
4702 683 248
BbvCI CC'TCA_GC
Cuts at: 962 1012 1115 1352 2032 962
Size: 50 103 237 680 4563
Fragments arranged by size:
4563 680 237 103 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5599
BciVI GTATCCnnnnn_n'
Cuts at: 3531 4587 5633 3531
Size: 1056 1046 3531
Fragments arranged by size:
3531 1056 1046
BclI T'GATC_A
Cuts at: 351 3153 351
Size: 2802 2831
BglII A'GATC_T
Cuts at: 2316 3148 5185 2316
Size: 832 2037 2764
Fragments arranged by size:
2764 2037 832
BlpI GC'TnA_GC
Cuts at: 1456 1670 2745 1456
Size: 214 1075 4344
Fragments arranged by size:
4344 1075 214
Bme1580I G_kGCm'C
Cuts at: 846 2201 3258 3351 4475 846
Size: 1355 1057 93 1124 2004
Fragments arranged by size:
2004 1355 1124 1057 93
BmrI ACTGGGnnnn_n'
Cuts at: 368 870 1995 2845 2926 3261 5405 368
Size: 502 1125 850 81 335 2144 596
Fragments arranged by size:
2144 1125 850 596 502 335 81
BmtI G_CTAG'C
Cuts at: 743 743
Size: 5633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 688 804 1946 1991 2326 2441 2629 108
Size: 580 116 1142 45 335 115 188 3112
Fragments arranged by size:
3112 1142 580 335 188 116 115 45
Bpu10I CC'TnA_GC
Cuts at: 962 1012 1115 1352 2032 2408 962
Size: 50 103 237 680 376 4187
Fragments arranged by size:
4187 680 376 237 103 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 609 2383 2809 3688 4402 4700 454
Size: 155 1774 426 879 714 298 1387
Fragments arranged by size:
1774 1387 879 714 426 298 155
BsaI GGTCTCn'nnnn_
Cuts at: 1922 5337 1922
Size: 3415 2218
BsaAI yAC'GTr
Cuts at: 2986 2986
Size: 5633
BsaBI GATnn'nnATC
Cuts at: 6 3147 5184 6
Size: 3141 2037 455
Fragments arranged by size:
3141 2037 455
BsaHI Gr'CG_yC
Cuts at: 2381 3064 3312 4014 5510 2381
Size: 683 248 702 1496 2504
Fragments arranged by size:
2504 1496 702 683 248
BsaWI w'CCGG_w
Cuts at: 299 710 2822 3343 4021 4432 4579 4853 299
Size: 411 2112 521 678 411 147 274 1079
Fragments arranged by size:
2112 1079 678 521 411 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1872 1902 1989 2019 1872
Size: 30 87 30 5486
Fragments arranged by size:
5486 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1482 1482
Size: 5633
BseYI C'CCAG_C
Cuts at: 908 1106 1361 1626 1847 2509 3058 3816 4481
Size: 198 255 265 221 662 549 758 665
Cuts at: 4481 4889 908
Size: 408 1652
Fragments arranged by size:
1652 758 665 662 549 408 265 255
221 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2028 2028
Size: 5633
BsiEI CG_ry'CG
Cuts at: 104 3175 3221 4451 104
Size: 3071 46 1230 1286
Fragments arranged by size:
3071 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2201 2647 3425 3615 4475 2201
Size: 446 778 190 860 3359
Fragments arranged by size:
3359 860 778 446 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2548 2548
Size: 5633
Bsp1286I G_dGCh'C
Cuts at: 846 1690 2201 2647 3258 3351 3425 3615 3677
Size: 844 511 446 611 93 74 190 62
Cuts at: 3677 4475 846
Size: 798 2004
Fragments arranged by size:
2004 844 798 611 511 446 190 93
74 62
BspEI T'CCGG_A
Cuts at: 710 710
Size: 5633
BspHI T'CATG_A
Cuts at: 4065 4065
Size: 5633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1674 2683 2897 3199 3580 1674
Size: 1009 214 302 381 3727
Fragments arranged by size:
3727 1009 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3925 3979 5463 5628 50
Size: 3875 54 1484 165 55
Fragments arranged by size:
3875 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3545 3545
Size: 5633
BsrFI r'CCGG_y
Cuts at: 3631 3812 3631
Size: 181 5452
BsrGI T'GTAC_A
Cuts at: 1934 2486 1934
Size: 552 5081
BssSI C'ACGA_G
Cuts at: 3904 4612 3904
Size: 708 4925
BstAPI GCAn_nnn'nTGC
Cuts at: 841 1209 1587 2042 3010 841
Size: 368 378 455 968 3464
Fragments arranged by size:
3464 968 455 378 368
BstBI TT'CG_AA
Cuts at: 2574 3994 2574
Size: 1420 4213
BstEII G'GTnAC_C
Cuts at: 864 864
Size: 5633
BstXI CCAn_nnnn'nTGG
Cuts at: 680 1369 2271 2517 680
Size: 689 902 246 3796
Fragments arranged by size:
3796 902 689 246
BstYI r'GATC_y
Cuts at: 1 815 1244 2316 2387 2603 2658 3125 3148
Size: 814 429 1072 71 216 55 467 23
Cuts at: 3148 3483 3729 4035 4047 4133 5185 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1072 1052 814 467 449 429 335 306
246 216 86 71 55 23 12
Bsu36I CC'TnA_GG
Cuts at: 2324 2599 2324
Size: 275 5358
BtgI C'CryG_G
Cuts at: 1142 1868 2175 1142
Size: 726 307 4600
Fragments arranged by size:
4600 726 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3583 3764 3927 3583
Size: 181 163 5289
Fragments arranged by size:
5289 181 163
BtsI GCAGTG_nn'
Cuts at: 810 1516 2043 2876 810
Size: 706 527 833 3567
Fragments arranged by size:
3567 833 706 527
DraI TTT'AAA
Cuts at: 2632 2632
Size: 5633
DraIII CAC_nnn'GTG
Cuts at: 841 1317 2107 841
Size: 476 790 4367
Fragments arranged by size:
4367 790 476
DrdI GACnn_nn'nnGTC
Cuts at: 3339 4683 3339
Size: 1344 4289
EaeI y'GGCC_r
Cuts at: 513 2178 2460 2579 3218 3392 3783 3810 5155
Size: 1665 282 119 639 174 391 27 1345
Cuts at: 5155 513
Size: 991
Fragments arranged by size:
1665 1345 991 639 391 282 174 119
27
EagI C'GGCC_G
Cuts at: 3218 3218
Size: 5633
EarI CTCTTCn'nnn_
Cuts at: 2362 3656 3866 2362
Size: 1294 210 4129
Fragments arranged by size:
4129 1294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 3841 4583 4729 5443 2362
Size: 1479 742 146 714 2552
Fragments arranged by size:
2552 1479 742 714 146
EcoICRI GAG'CTC
Cuts at: 2645 2645
Size: 5633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 688 732 804 1079 1946 1991 2239 2326
Size: 580 44 72 275 867 45 248 87
Cuts at: 2326 2441 2629 3458 3890 4243 4905 108
Size: 115 188 829 432 353 662 836
Fragments arranged by size:
867 836 829 662 580 432 353 275
248 188 115 87 72 45 44
EcoNI CCTnn'n_nnAGG
Cuts at: 1694 1694
Size: 5633
EcoO109I rG'GnC_Cy
Cuts at: 1449 2772 1449
Size: 1323 4310
EcoRI G'AATT_C
Cuts at: 2637 2637
Size: 5633
EcoRV GAT'ATC
Cuts at: 526 526
Size: 5633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 5633
FspI TGC'GCA
Cuts at: 3414 3414
Size: 5633
HaeII r_GCGC'y
Cuts at: 2118 2384 3067 3315 4545 5316 2118
Size: 266 683 248 1230 771 2435
Fragments arranged by size:
2435 1230 771 683 266 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 713 745 1026 1058 2234 2266 3475
Size: 32 402 32 281 32 1176 32 1209
Cuts at: 3475 3505 3507 3537 279
Size: 30 2 30 2375
Fragments arranged by size:
2375 1209 1176 402 281 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2186 2672 2186
Size: 486 5147
KasI G'GCGC_C
Cuts at: 2380 3063 3311 2380
Size: 683 248 4702
Fragments arranged by size:
4702 683 248
KpnI G_GTAC'C
Cuts at: 1642 2653 1642
Size: 1011 4622
MluI A'CGCG_T
Cuts at: 5066 5066
Size: 5633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1310 4392 4576 1190
Size: 120 3082 184 2247
Fragments arranged by size:
3082 2247 184 120
MscI TGG'CCA
Cuts at: 515 2180 2581 3394 515
Size: 1665 401 813 2754
Fragments arranged by size:
2754 1665 813 401
MslI CAynn'nnrTG
Cuts at: 1969 2494 3622 3749 1969
Size: 525 1128 127 3853
Fragments arranged by size:
3853 1128 525 127
MspA1I CmG'CkG
Cuts at: 1066 1347 1633 1827 1911 2744 3058 3418 4202
Size: 281 286 194 84 833 314 360 784
Cuts at: 4202 4447 1066
Size: 245 2252
Fragments arranged by size:
2252 833 784 360 314 286 281 245
194 84
NaeI GCC'GGC
Cuts at: 3814 3814
Size: 5633
NarI GG'CG_CC
Cuts at: 2381 3064 3312 2381
Size: 683 248 4702
Fragments arranged by size:
4702 683 248
NcoI C'CATG_G
Cuts at: 1868 2175 1868
Size: 307 5326
NgoMIV G'CCGG_C
Cuts at: 3812 3812
Size: 5633
NheI G'CTAG_C
Cuts at: 739 739
Size: 5633
NspI r_CATG'y
Cuts at: 720 1230 2686 3186 4789 720
Size: 510 1456 500 1603 1564
Fragments arranged by size:
1603 1564 1456 510 500
PasI CC'CwG_GG
Cuts at: 1218 1218
Size: 5633
PciI A'CATG_T
Cuts at: 4785 4785
Size: 5633
PfoI T'CCnGG_A
Cuts at: 2606 2606
Size: 5633
PmeI GTTT'AAAC
Cuts at: 2632 2632
Size: 5633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 4079
Size: 32 5601
PstI C_TGCA'G
Cuts at: 1080 1759 2680 3365 1080
Size: 679 921 685 3348
Fragments arranged by size:
3348 921 685 679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5633
PvuII CAG'CTG
Cuts at: 1066 1347 1827 3058 3418 1066
Size: 281 480 1231 360 3281
Fragments arranged by size:
3281 1231 480 360 281
RsrII CG'GwC_CG
Cuts at: 3828 3828
Size: 5633
SacI G_AGCT'C
Cuts at: 2647 2647
Size: 5633
SalI G'TCGA_C
Cuts at: 2670 2670
Size: 5633
SapI GCTCTTCn'nnn_
Cuts at: 3656 3866 3656
Size: 210 5423
SbfI CC_TGCA'GG
Cuts at: 2680 2680
Size: 5633
ScaI AGT'ACT
Cuts at: 1783 5150 1783
Size: 3367 2266
SexAI A'CCwGG_T
Cuts at: 860 1806 860
Size: 946 4687
SfcI C'TryA_G
Cuts at: 33 1076 1323 1650 1694 1755 1896 2676 3361
Size: 1043 247 327 44 61 141 780 685
Cuts at: 3361 4329 4520 33
Size: 968 191 1146
Fragments arranged by size:
1146 1043 968 780 685 327 247 191
141 61 44
SfoI GGC'GCC
Cuts at: 2382 3065 3313 2382
Size: 683 248 4702
Fragments arranged by size:
4702 683 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5633
SmaI CCC'GGG
Cuts at: 2655 2655
Size: 5633
SmlI C'TyrA_G
Cuts at: 469 624 2398 2788 3703 4417 4679 469
Size: 155 1774 390 915 714 262 1423
Fragments arranged by size:
1774 1423 915 714 390 262 155
SpeI A'CTAG_T
Cuts at: 67 385 67
Size: 318 5315
SphI G_CATG'C
Cuts at: 720 2686 3186 720
Size: 1966 500 3167
Fragments arranged by size:
3167 1966 500
StuI AGG'CCT
Cuts at: 1496 1622 1496
Size: 126 5507
StyI C'CwwG_G
Cuts at: 196 679 1047 1413 1868 2175 2767 3121 196
Size: 483 368 366 455 307 592 354 2708
Fragments arranged by size:
2708 592 483 455 368 366 354 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 509 969 3216 3349 3808 4017 509
Size: 460 2247 133 459 209 2125
Fragments arranged by size:
2247 2125 460 459 209 133
TatI w'GTAC_w
Cuts at: 901 1508 1646 1781 1934 2144 2486 5148 901
Size: 607 138 135 153 210 342 2662 1386
Fragments arranged by size:
2662 1386 607 342 210 153 138 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5150 5182 5150
Size: 32 5601
Tth111I GACn'n_nGTC
Cuts at: 2054 3430 2054
Size: 1376 4257
XbaI T'CTAG_A
Cuts at: 2664 2664
Size: 5633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 828 1420 2209 3128 828
Size: 592 789 919 3333
Fragments arranged by size:
3333 919 789 592
XmaI C'CCGG_G
Cuts at: 2653 2653
Size: 5633
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI ApaI AscI AvrII
BbsI BfrBI BglI BmgBI BsiWI BsmI BssHII BstZ17I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI