Restriction map for
pF1KA1000
(Circular) (Six-base) MAPSORT of: pF1KA1000.seq Check: 7427 from: 1 to: 8951
pF1KA1000 8951 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5037 5142 5037
Size: 105 8846
AccI GT'mk_AC
Cuts at: 2190 5989 2190
Size: 3799 5152
Acc65I G'GTAC_C
Cuts at: 1899 5967 1899
Size: 4068 4883
AclI AA'CG_TT
Cuts at: 8791 8791
Size: 8951
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1142 2390 2654 2719 2794 2800 3238 3439
Size: 646 1248 264 65 75 6 438 201
Cuts at: 3439 3460 4973 5005 5065 5164 5170 5387 5470
Size: 21 1513 32 60 99 6 217 83
Cuts at: 5470 5558 5740 6776 7208 7955 8223 496
Size: 88 182 1036 432 747 268 1224
Fragments arranged by size:
1513 1248 1224 1036 747 646 438 432
268 264 217 201 182 99 88 83
75 65 60 32 21 6 6
AfeI AGC'GCT
Cuts at: 3907 3907
Size: 8951
AflIII A'CryG_T
Cuts at: 1619 6303 7407 8384 1619
Size: 4684 1104 977 2186
Fragments arranged by size:
4684 2186 1104 977
AgeI A'CCGG_T
Cuts at: 7339 7339
Size: 8951
AhdI GACnn_n'nnGTC
Cuts at: 4428 4428
Size: 8951
AleI CACnn'nnGTG
Cuts at: 902 2428 902
Size: 1526 7425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 920 2548 920
Size: 1628 7323
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1808 1840 1808
Size: 32 8919
AlwNI CAG_nnn'CTG
Cuts at: 1099 3162 3663 3792 6256 7823 1099
Size: 2063 501 129 2464 1567 2227
Fragments arranged by size:
2464 2227 2063 1567 501 129
ApaI G_GGCC'C
Cuts at: 1482 1482
Size: 8951
ApaLI G'TGCA_C
Cuts at: 2711 7721 2711
Size: 5010 3941
ApoI r'AATT_y
Cuts at: 883 1010 1231 1858 2542 2940 3414 3652 4896
Size: 127 221 627 684 398 474 238 1244
Cuts at: 4896 5955 8304 8560 883
Size: 1059 2349 256 1274
Fragments arranged by size:
2349 1274 1244 1059 684 627 474 398
256 238 221 127
AseI AT'TA_AT
Cuts at: 20 1577 20
Size: 1557 7394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8951
AvaI C'yCGr_G
Cuts at: 3527 3751 4417 4438 5971 3527
Size: 224 666 21 1533 6507
Fragments arranged by size:
6507 1533 666 224 21
BamHI G'GATC_C
Cuts at: 3607 5976 3607
Size: 2369 6582
BanI G'GyrC_C
Cuts at: 904 1417 1899 3250 4397 5967 6381 6629 6664
Size: 513 482 1351 1147 1570 414 248 35
Cuts at: 6664 904
Size: 3191
Fragments arranged by size:
3191 1570 1351 1147 513 482 414 248
35
BanII G_rGCy'C
Cuts at: 1482 3752 4418 4586 4794 5261 5664 5965 6995
Size: 2270 666 168 208 467 403 301 1030
Cuts at: 6995 1482
Size: 3438
Fragments arranged by size:
3438 2270 1030 666 467 403 301 208
168
BbeI G_GCGC'C
Cuts at: 6385 6633 6385
Size: 248 8703
BbsI GAAGACnn'nnnn_
Cuts at: 431 1970 2487 3466 4088 4301 4759 5717 431
Size: 1539 517 979 622 213 458 958 3665
Fragments arranged by size:
3665 1539 979 958 622 517 458 213
BbvCI CC'TCA_GC
Cuts at: 305 3382 5314 305
Size: 3077 1932 3942
Fragments arranged by size:
3942 3077 1932
BciVI GTATCCnnnnn_n'
Cuts at: 2787 6849 7610 8951 2787
Size: 4062 761 1341 2787
Fragments arranged by size:
4062 2787 1341 761
BclI T'GATC_A
Cuts at: 6471 6471
Size: 8951
BfrBI ATG'CAT
Cuts at: 2159 5681 2159
Size: 3522 5429
BglI GCCn_nnn'nGGC
Cuts at: 5413 5425 5413
Size: 12 8939
BglII A'GATC_T
Cuts at: 1707 6466 8503 1707
Size: 4759 2037 2155
Fragments arranged by size:
4759 2155 2037
BlpI GC'TnA_GC
Cuts at: 221 2822 3193 3449 3620 3746 4151 4208 5029
Size: 2601 371 256 171 126 405 57 821
Cuts at: 5029 5165 6063 221
Size: 136 898 3109
Fragments arranged by size:
3109 2601 898 821 405 371 256 171
136 126 57
Bme1580I G_kGCm'C
Cuts at: 1422 1482 2715 3255 4128 6576 6669 7725 1422
Size: 60 1233 540 873 2448 93 1056 2648
Fragments arranged by size:
2648 2448 1233 1056 873 540 93 60
BmrI ACTGGGnnnn_n'
Cuts at: 2400 6163 6244 6579 8723 2400
Size: 3763 81 335 2144 2628
Fragments arranged by size:
3763 2628 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3574 7318 3574
Size: 3744 5207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1001 1223 1651 1816 2908 3328 3613 4432
Size: 612 222 428 165 1092 420 285 819
Cuts at: 4432 5008 5353 5641 389
Size: 576 345 288 3699
Fragments arranged by size:
3699 1092 819 612 576 428 420 345
288 285 222 165
Bpu10I CC'TnA_GC
Cuts at: 305 3202 3382 3866 5314 305
Size: 2897 180 484 1448 3942
Fragments arranged by size:
3942 2897 1448 484 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 1582 3265 3277 4573 4579 5692 5873 6127
Size: 15 1683 12 1296 6 1113 181 254
Cuts at: 6127 7006 7498 7796 1567
Size: 879 492 298 2722
Fragments arranged by size:
2722 1683 1296 1113 879 492 298 254
181 15 12 6
BsaI GGTCTCn'nnnn_
Cuts at: 523 4102 8655 523
Size: 3579 4553 819
Fragments arranged by size:
4553 3579 819
BsaAI yAC'GTr
Cuts at: 6304 6304
Size: 8951
BsaBI GATnn'nnATC
Cuts at: 6 2536 5089 6465 8502 6
Size: 2530 2553 1376 2037 455
Fragments arranged by size:
2553 2530 2037 1376 455
BsaHI Gr'CG_yC
Cuts at: 4424 6382 6630 7332 8828 4424
Size: 1958 248 702 1496 4547
Fragments arranged by size:
4547 1958 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4217 6140 6661 7339 7613 7760 8171 4217
Size: 1923 521 678 274 147 411 4997
Fragments arranged by size:
4997 1923 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1066 1096 2672 2702 3978 4008 1066
Size: 30 1576 30 1276 30 6009
Fragments arranged by size:
6009 1576 1276 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 858 1308 2947 3196 3199 3610 4540 4576 4624
Size: 450 1639 249 3 411 930 36 48
Cuts at: 4624 5302 858
Size: 678 4507
Fragments arranged by size:
4507 1639 930 678 450 411 249 48
36 3
BseYI C'CCAG_C
Cuts at: 2443 6376 7134 7711 8207 2443
Size: 3933 758 577 496 3187
Fragments arranged by size:
3933 3187 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3157 3160 3283 3649 3676 4198 5398 5663 3157
Size: 3 123 366 27 522 1200 265 6445
Fragments arranged by size:
6445 1200 522 366 265 123 27 3
BsiEI CG_ry'CG
Cuts at: 104 6493 6539 7747 104
Size: 6389 46 1208 1308
Fragments arranged by size:
6389 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2520 2715 3062 3752 4418 4586 5261 5965 6743
Size: 195 347 690 666 168 675 704 778
Cuts at: 6743 6933 7725 2520
Size: 190 792 3746
Fragments arranged by size:
3746 792 778 704 690 675 666 347
195 190 168
BsmI GAATG_Cn'
Cuts at: 294 1517 2312 294
Size: 1223 795 6933
Fragments arranged by size:
6933 1223 795
Bsp1286I G_dGCh'C
Cuts at: 1422 1482 2520 2715 3062 3255 3752 4128 4418
Size: 60 1038 195 347 193 497 376 290
Cuts at: 4418 4586 4794 5261 5664 5965 6576 6669 6743
Size: 168 208 467 403 301 611 93 74
Cuts at: 6743 6933 6995 7725 1422
Size: 190 62 730 2648
Fragments arranged by size:
2648 1038 730 611 497 467 403 376
347 301 290 208 195 193 190 168
93 74 62 60
BspEI T'CCGG_A
Cuts at: 8171 8171
Size: 8951
BspHI T'CATG_A
Cuts at: 1035 8127 1035
Size: 7092 1859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1049 3179 3768 5037 5142 6001 6215 6517 6898
Size: 2130 589 1269 105 859 214 302 381
Cuts at: 6898 1049
Size: 3102
Fragments arranged by size:
3102 2130 1269 859 589 381 302 214
105
BsrBI CCG'CTC
Cuts at: 50 4451 5169 7243 7297 8781 8946 50
Size: 4401 718 2074 54 1484 165 55
Fragments arranged by size:
4401 2074 1484 718 165 55 54
BsrDI GCAATG_nn'
Cuts at: 440 4258 5468 6863 440
Size: 3818 1210 1395 2528
Fragments arranged by size:
3818 2528 1395 1210
BsrFI r'CCGG_y
Cuts at: 5042 6949 7130 7339 5042
Size: 1907 181 209 6654
Fragments arranged by size:
6654 1907 209 181
BssSI C'ACGA_G
Cuts at: 4522 7222 7580 4522
Size: 2700 358 5893
Fragments arranged by size:
5893 2700 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4404 6328 4404
Size: 1924 7027
BstBI TT'CG_AA
Cuts at: 7312 7312
Size: 8951
BstEII G'GTnAC_C
Cuts at: 1401 4974 1401
Size: 3573 5378
BstXI CCAn_nnnn'nTGG
Cuts at: 249 979 4359 5070 249
Size: 730 3380 711 4130
Fragments arranged by size:
4130 3380 730 711
BstYI r'GATC_y
Cuts at: 1 171 1707 2879 3607 5202 5976 6443 6466
Size: 170 1536 1172 728 1595 774 467 23
Cuts at: 6466 6801 7047 8059 8145 8157 8503 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1595 1536 1172 1012 774 728 467 449
346 335 246 170 86 23 12
BstZ17I GTA'TAC
Cuts at: 2191 2191
Size: 8951
Bsu36I CC'TnA_GG
Cuts at: 1766 1766
Size: 8951
BtgI C'CryG_G
Cuts at: 107 1148 4352 107
Size: 1041 3204 4706
Fragments arranged by size:
4706 3204 1041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5691 6901 7082 7245 5691
Size: 1210 181 163 7397
Fragments arranged by size:
7397 1210 181 163
BtsI GCAGTG_nn'
Cuts at: 2860 4921 5646 6194 2860
Size: 2061 725 548 5617
Fragments arranged by size:
5617 2061 725 548
ClaI AT'CG_AT
Cuts at: 3005 3005
Size: 8951
DraI TTT'AAA
Cuts at: 1236 2298 2439 3320 5950 1236
Size: 1062 141 881 2630 4237
Fragments arranged by size:
4237 2630 1062 881 141
DrdI GACnn_nn'nnGTC
Cuts at: 6657 7515 6657
Size: 858 8093
EaeI y'GGCC_r
Cuts at: 489 578 1133 4361 5235 6536 6710 7101 7128
Size: 89 555 3228 874 1301 174 391 27
Cuts at: 7128 8473 489
Size: 1345 967
Fragments arranged by size:
3228 1345 1301 967 874 555 391 174
89 27
EagI C'GGCC_G
Cuts at: 6536 6536
Size: 8951
EarI CTCTTCn'nnn_
Cuts at: 526 589 691 1247 1745 2644 2699 2843 2924
Size: 63 102 556 498 899 55 144 81
Cuts at: 2924 3371 3674 3718 3896 4013 4658 4742 4994
Size: 447 303 44 178 117 645 84 252
Cuts at: 4994 5186 5249 5363 5393 6974 7184 526
Size: 192 63 114 30 1581 210 2293
Fragments arranged by size:
2293 1581 899 645 556 498 447 303
252 210 192 178 144 117 114 102
84 81 63 63 55 44 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2979 5812 7159 7469 7615 8761 2979
Size: 2833 1347 310 146 1146 3169
Fragments arranged by size:
3169 2833 1347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3750 4416 4584 5259 5963 3750
Size: 666 168 675 704 6738
Fragments arranged by size:
6738 704 675 666 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 496 1001 1142 1223 1651 1816 2390 2654
Size: 107 505 141 81 428 165 574 264
Cuts at: 2654 2719 2794 2800 2908 3238 3328 3439 3460
Size: 65 75 6 108 330 90 111 21
Cuts at: 3460 3613 4432 4973 5005 5008 5065 5164 5170
Size: 153 819 541 32 3 57 99 6
Cuts at: 5170 5353 5387 5470 5558 5641 5740 6776 7208
Size: 183 34 83 88 83 99 1036 432
Cuts at: 7208 7955 8223 389
Size: 747 268 1117
Fragments arranged by size:
1117 1036 819 747 574 541 505 432
428 330 268 264 183 165 153 141
111 108 107 99 99 90 88 83
83 81 75 65 57 34 32 21
6 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 451 2631 2785 3259 4144 4441 451
Size: 2180 154 474 885 297 4961
Fragments arranged by size:
4961 2180 885 474 297 154
EcoO109I rG'GnC_Cy
Cuts at: 137 1478 1479 2327 4497 5004 5097 5210 6090
Size: 1341 1 848 2170 507 93 113 880
Cuts at: 6090 137
Size: 2998
Fragments arranged by size:
2998 2170 1341 880 848 507 113 93
1
EcoRI G'AATT_C
Cuts at: 3414 5955 3414
Size: 2541 6410
EcoRV GAT'ATC
Cuts at: 939 3108 939
Size: 2169 6782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 2395 3828 4080 4873 6970 1960
Size: 435 1433 252 793 2097 3941
Fragments arranged by size:
3941 2097 1433 793 435 252
FspI TGC'GCA
Cuts at: 6732 6732
Size: 8951
HaeII r_GCGC'y
Cuts at: 486 3909 6385 6633 7655 8634 486
Size: 3423 2476 248 1022 979 803
Fragments arranged by size:
3423 2476 1022 979 803 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 387 419 853 885 2109 2141 2405 2437 2470
Size: 32 434 32 1224 32 264 32 33
Cuts at: 2470 2502 2640 2672 4099 4131 4414 4446 4659
Size: 32 138 32 1427 32 283 32 213
Cuts at: 4659 4691 5194 5226 5724 5756 6793 6823 6825
Size: 32 503 32 498 32 1037 30 2
Cuts at: 6825 6855 387
Size: 30 2483
Fragments arranged by size:
2483 1427 1224 1037 503 498 434 283
264 213 138 33 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 5990 5990
Size: 8951
HindIII A'AGCT_T
Cuts at: 1697 4867 1697
Size: 3170 5781
KasI G'GCGC_C
Cuts at: 6381 6629 6381
Size: 248 8703
KpnI G_GTAC'C
Cuts at: 1903 5971 1903
Size: 4068 4883
MfeI C'AATT_G
Cuts at: 1611 1611
Size: 8951
MluI A'CGCG_T
Cuts at: 8384 8384
Size: 8951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2891 7622 7806 2006
Size: 885 4731 184 3151
Fragments arranged by size:
4731 3151 885 184
MscI TGG'CCA
Cuts at: 491 1135 4363 6712 491
Size: 644 3228 2349 2730
Fragments arranged by size:
3228 2730 2349 644
MslI CAynn'nnrTG
Cuts at: 433 449 892 902 2428 2611 4357 6940 7067
Size: 16 443 10 1526 183 1746 2583 127
Cuts at: 7067 433
Size: 2317
Fragments arranged by size:
2583 2317 1746 1526 443 183 127 16
10
MspA1I CmG'CkG
Cuts at: 2368 2886 3624 4098 4404 4410 4482 5112 6062
Size: 518 738 474 306 6 72 630 950
Cuts at: 6062 6376 6736 7749 7994 2368
Size: 314 360 1013 245 3325
Fragments arranged by size:
3325 1013 950 738 630 518 474 360
314 306 245 72 6
NaeI GCC'GGC
Cuts at: 7132 7132
Size: 8951
NarI GG'CG_CC
Cuts at: 6382 6630 6382
Size: 248 8703
NcoI C'CATG_G
Cuts at: 107 1148 4352 107
Size: 1041 3204 4706
Fragments arranged by size:
4706 3204 1041
NgoMIV G'CCGG_C
Cuts at: 7130 7130
Size: 8951
NsiI A_TGCA'T
Cuts at: 2161 5683 2161
Size: 3522 5429
NspI r_CATG'y
Cuts at: 657 918 1623 3733 6004 6504 7411 657
Size: 261 705 2110 2271 500 907 2197
Fragments arranged by size:
2271 2197 2110 907 705 500 261
PasI CC'CwG_GG
Cuts at: 5070 5070
Size: 8951
PciI A'CATG_T
Cuts at: 1619 7407 1619
Size: 5788 3163
PflMI CCAn_nnn'nTGG
Cuts at: 3570 3570
Size: 8951
PfoI T'CCnGG_A
Cuts at: 772 5261 772
Size: 4489 4462
PmeI GTTT'AAAC
Cuts at: 5950 5950
Size: 8951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8090 8122 8090
Size: 32 8919
PpuMI rG'GwC_Cy
Cuts at: 2327 5004 5097 5210 2327
Size: 2677 93 113 6068
Fragments arranged by size:
6068 2677 113 93
PshAI GACnn'nnGTC
Cuts at: 4759 4759
Size: 8951
PsiI TTA'TAA
Cuts at: 1907 1907
Size: 8951
PspOMI G'GGCC_C
Cuts at: 1478 1478
Size: 8951
PspXI vC'TCGA_Gb
Cuts at: 3527 3751 3527
Size: 224 8727
PstI C_TGCA'G
Cuts at: 2285 4145 4409 4458 4487 4937 5998 6683 2285
Size: 1860 264 49 29 450 1061 685 4553
Fragments arranged by size:
4553 1860 1061 685 450 264 49 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8951
PvuII CAG'CTG
Cuts at: 2368 2886 3624 4098 4404 4410 4482 5112 6376
Size: 518 738 474 306 6 72 630 1264
Cuts at: 6376 6736 2368
Size: 360 4583
Fragments arranged by size:
4583 1264 738 630 518 474 360 306
72 6
RsrII CG'GwC_CG
Cuts at: 7146 7146
Size: 8951
SacI G_AGCT'C
Cuts at: 3752 4418 4586 5261 5965 3752
Size: 666 168 675 704 6738
Fragments arranged by size:
6738 704 675 666 168
SalI G'TCGA_C
Cuts at: 5988 5988
Size: 8951
SapI GCTCTTCn'nnn_
Cuts at: 1247 2644 2843 3371 3896 5249 6974 7184 1247
Size: 1397 199 528 525 1353 1725 210 3014
Fragments arranged by size:
3014 1725 1397 1353 528 525 210 199
SbfI CC_TGCA'GG
Cuts at: 5998 5998
Size: 8951
ScaI AGT'ACT
Cuts at: 2022 8468 2022
Size: 6446 2505
SexAI A'CCwGG_T
Cuts at: 2184 4685 2184
Size: 2501 6450
SfcI C'TryA_G
Cuts at: 33 235 1391 1772 2212 2281 3053 3772 4141
Size: 202 1156 381 440 69 772 719 369
Cuts at: 4141 4405 4454 4483 4933 5994 6679 7672 7863
Size: 264 49 29 450 1061 685 993 191
Cuts at: 7863 33
Size: 1121
Fragments arranged by size:
1156 1121 1061 993 772 719 685 450
440 381 369 264 202 191 69 49
29
SfoI GGC'GCC
Cuts at: 6383 6631 6383
Size: 248 8703
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8951
SmaI CCC'GGG
Cuts at: 5973 5973
Size: 8951
SmlI C'TyrA_G
Cuts at: 1546 1561 3244 3256 3527 3751 4588 4594 5671
Size: 15 1683 12 271 224 837 6 1077
Cuts at: 5671 5888 6106 7021 7513 7775 1546
Size: 217 218 915 492 262 2722
Fragments arranged by size:
2722 1683 1077 915 837 492 271 262
224 218 217 15 12 6
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8951
SphI G_CATG'C
Cuts at: 918 6004 6504 918
Size: 5086 500 3365
Fragments arranged by size:
5086 3365 500
StuI AGG'CCT
Cuts at: 3440 3440
Size: 8951
StyI C'CwwG_G
Cuts at: 107 183 248 1148 2323 2387 2729 3131 4352
Size: 76 65 900 1175 64 342 402 1221
Cuts at: 4352 4382 6085 6439 107
Size: 30 1703 354 2619
Fragments arranged by size:
2619 1703 1221 1175 900 402 354 342
76 65 64 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1656 3577 6534 6667 7126 7335 1656
Size: 1921 2957 133 459 209 3272
Fragments arranged by size:
3272 2957 1921 459 209 133
TatI w'GTAC_w
Cuts at: 2020 3889 4576 8466 2020
Size: 1869 687 3890 2505
Fragments arranged by size:
3890 2505 1869 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 701 733 1066 1098 2244 2276 3978 4010 4513
Size: 32 333 32 1146 32 1702 32 503
Cuts at: 4513 4545 8468 8500 701
Size: 32 3923 32 1152
Fragments arranged by size:
3923 1702 1152 1146 503 333 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6748 6748
Size: 8951
XbaI T'CTAG_A
Cuts at: 5982 5982
Size: 8951
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1155 1989 2413 2883 4317 5475 6446 1155
Size: 834 424 470 1434 1158 971 3660
Fragments arranged by size:
3660 1434 1158 971 834 470 424
XhoI C'TCGA_G
Cuts at: 3527 3751 3527
Size: 224 8727
XmaI C'CCGG_G
Cuts at: 5971 5971
Size: 8951
XmnI GAAnn'nnTTC
Cuts at: 2267 4869 2267
Size: 2602 6349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BcgI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII CspCI DraIII FseI FspAI
HpaI NdeI NheI NotI NruI PacI PmlI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI