Restriction map for
pF1KA0987
(Circular) (Six-base) MAPSORT of: pF1KA0987.seq Check: 6436 from: 1 to: 6374
pF1KA0987 6374 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1763 2645 1763
Size: 882 5492
AccI GT'mk_AC
Cuts at: 3412 3412
Size: 6374
Acc65I G'GTAC_C
Cuts at: 3390 3390
Size: 6374
AclI AA'CG_TT
Cuts at: 1416 6214 1416
Size: 4798 1576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 2471 2487 4199 4631 5378 5646 719
Size: 1752 16 1712 432 747 268 1447
Fragments arranged by size:
1752 1712 1447 747 432 268 16
AflIII A'CryG_T
Cuts at: 3305 3726 4830 5807 3305
Size: 421 1104 977 3872
Fragments arranged by size:
3872 1104 977 421
AgeI A'CCGG_T
Cuts at: 4762 4762
Size: 6374
AhdI GACnn_n'nnGTC
Cuts at: 747 747
Size: 6374
AleI CACnn'nnGTG
Cuts at: 277 1507 2705 277
Size: 1230 1198 3946
Fragments arranged by size:
3946 1230 1198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 2438 295
Size: 2143 4231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2429 2461 2429
Size: 32 6342
AlwNI CAG_nnn'CTG
Cuts at: 240 685 2353 2869 3264 3679 5246 240
Size: 445 1668 516 395 415 1567 1368
Fragments arranged by size:
1668 1567 1368 516 445 415 395
ApaLI G'TGCA_C
Cuts at: 2629 2698 3122 5144 2629
Size: 69 424 2022 3859
Fragments arranged by size:
3859 2022 424 69
ApoI r'AATT_y
Cuts at: 667 1300 2536 3014 3378 5727 5983 667
Size: 633 1236 478 364 2349 256 1058
Fragments arranged by size:
2349 1236 1058 633 478 364 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6374
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6374
AvaI C'yCGr_G
Cuts at: 2151 3394 2151
Size: 1243 5131
AvrII C'CTAG_G
Cuts at: 1803 1803
Size: 6374
BamHI G'GATC_C
Cuts at: 3399 3399
Size: 6374
BanI G'GyrC_C
Cuts at: 198 3390 3804 4052 4087 198
Size: 3192 414 248 35 2485
Fragments arranged by size:
3192 2485 414 248 35
BanII G_rGCy'C
Cuts at: 164 796 818 1691 1769 2114 2255 2791 3388
Size: 632 22 873 78 345 141 536 597
Cuts at: 3388 4418 164
Size: 1030 2120
Fragments arranged by size:
2120 1030 873 632 597 536 345 141
78 22
BbeI G_GCGC'C
Cuts at: 202 3808 4056 202
Size: 3606 248 2520
Fragments arranged by size:
3606 2520 248
BbsI GAAGACnn'nnnn_
Cuts at: 362 905 2459 2968 362
Size: 543 1554 509 3768
Fragments arranged by size:
3768 1554 543 509
BbvCI CC'TCA_GC
Cuts at: 1529 1529
Size: 6374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3012 3046 3012
Size: 34 6340
BciVI GTATCCnnnnn_n'
Cuts at: 1912 4272 5033 6374 1912
Size: 2360 761 1341 1912
Fragments arranged by size:
2360 1912 1341 761
BclI T'GATC_A
Cuts at: 1499 3894 1499
Size: 2395 3979
BfrBI ATG'CAT
Cuts at: 960 960
Size: 6374
BglII A'GATC_T
Cuts at: 3096 3889 5926 3096
Size: 793 2037 3544
Fragments arranged by size:
3544 2037 793
BlpI GC'TnA_GC
Cuts at: 1748 2644 3486 1748
Size: 896 842 4636
Fragments arranged by size:
4636 896 842
Bme1580I G_kGCm'C
Cuts at: 1671 2009 2633 2702 3126 3999 4092 5148 1671
Size: 338 624 69 424 873 93 1056 2897
Fragments arranged by size:
2897 1056 873 624 424 338 93 69
BmrI ACTGGGnnnn_n'
Cuts at: 3586 3667 4002 6146 3586
Size: 81 335 2144 3814
Fragments arranged by size:
3814 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 510 4741 510
Size: 4231 2143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 480 1781 2128 3121 262
Size: 218 1301 347 993 3515
Fragments arranged by size:
3515 1301 993 347 218
Bpu10I CC'TnA_GC
Cuts at: 611 1016 1529 3269 611
Size: 405 513 1740 3716
Fragments arranged by size:
3716 1740 513 405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2148 2239 2264 3550 4429 4921 5219 2148
Size: 91 25 1286 879 492 298 3303
Fragments arranged by size:
3303 1286 879 492 298 91 25
BsaI GGTCTCn'nnnn_
Cuts at: 407 2206 2662 6078 407
Size: 1799 456 3416 703
Fragments arranged by size:
3416 1799 703 456
BsaAI yAC'GTr
Cuts at: 569 3727 569
Size: 3158 3216
BsaBI GATnn'nnATC
Cuts at: 6 1007 3888 5925 6
Size: 1001 2881 2037 455
Fragments arranged by size:
2881 2037 1001 455
BsaHI Gr'CG_yC
Cuts at: 199 940 3805 4053 4755 6251 199
Size: 741 2865 248 702 1496 322
Fragments arranged by size:
2865 1496 741 702 322 248
BsaWI w'CCGG_w
Cuts at: 751 1515 3563 4084 4762 5036 5183 5594 751
Size: 764 2048 521 678 274 147 411 1531
Fragments arranged by size:
2048 1531 764 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1497 1527 2659 2689 3353 3383 1497
Size: 30 1132 30 664 30 4488
Fragments arranged by size:
4488 1132 664 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 1546 1549 2698 2842 2905 3014 238
Size: 1308 3 1149 144 63 109 3598
Fragments arranged by size:
3598 1308 1149 144 109 63 3
BseYI C'CCAG_C
Cuts at: 789 1383 2570 3799 4557 5134 5630 789
Size: 594 1187 1229 758 577 496 1533
Fragments arranged by size:
1533 1229 1187 758 594 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 2683 2921 901
Size: 1782 238 4354
Fragments arranged by size:
4354 1782 238
BsiEI CG_ry'CG
Cuts at: 104 116 1083 1165 3916 3962 5170 104
Size: 12 967 82 2751 46 1208 1308
Fragments arranged by size:
2751 1308 1208 967 82 46 12
BsiHKAI G_wGCw'C
Cuts at: 818 1691 1754 2255 2633 2702 3126 3388 4166
Size: 873 63 501 378 69 424 262 778
Cuts at: 4166 4356 5148 818
Size: 190 792 2044
Fragments arranged by size:
2044 873 792 778 501 424 378 262
190 69 63
BsmI GAATG_Cn'
Cuts at: 2761 2761
Size: 6374
BsmBI CGTCTCn'nnnn_
Cuts at: 2608 2608
Size: 6374
Bsp1286I G_dGCh'C
Cuts at: 164 796 818 1671 1691 1754 1769 2009 2114
Size: 632 22 853 20 63 15 240 105
Cuts at: 2114 2255 2633 2702 2791 3126 3388 3999 4092
Size: 141 378 69 89 335 262 611 93
Cuts at: 4092 4166 4356 4418 5148 164
Size: 74 190 62 730 1390
Fragments arranged by size:
1390 853 730 632 611 378 335 262
240 190 141 105 93 89 74 69
63 62 22 20 15
BspEI T'CCGG_A
Cuts at: 751 1515 5594 751
Size: 764 4079 1531
Fragments arranged by size:
4079 1531 764
BspHI T'CATG_A
Cuts at: 5550 5550
Size: 6374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1763 2645 3424 3638 3940 4321 1763
Size: 882 779 214 302 381 3816
Fragments arranged by size:
3816 882 779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1539 1991 2688 4666 4720 6204 6369 50
Size: 1489 452 697 1978 54 1484 165 55
Fragments arranged by size:
1978 1489 1484 697 452 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4286 4286
Size: 6374
BsrFI r'CCGG_y
Cuts at: 1082 4372 4553 4762 1082
Size: 3290 181 209 2694
Fragments arranged by size:
3290 2694 209 181
BssHII G'CGCG_C
Cuts at: 189 189
Size: 6374
BssSI C'ACGA_G
Cuts at: 1603 4645 5003 1603
Size: 3042 358 2974
Fragments arranged by size:
3042 2974 358
BstAPI GCAn_nnn'nTGC
Cuts at: 440 2637 3751 440
Size: 2197 1114 3063
Fragments arranged by size:
3063 2197 1114
BstBI TT'CG_AA
Cuts at: 633 4735 633
Size: 4102 2272
BstXI CCAn_nnnn'nTGG
Cuts at: 3324 3324
Size: 6374
BstYI r'GATC_y
Cuts at: 1 1157 3096 3399 3866 3889 4224 4470 5482
Size: 1156 1939 303 467 23 335 246 1012
Cuts at: 5482 5568 5580 5926 1
Size: 86 12 346 449
Fragments arranged by size:
1939 1156 1012 467 449 346 335 303
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4324 4505 4668 4324
Size: 181 163 6030
Fragments arranged by size:
6030 181 163
BtsI GCAGTG_nn'
Cuts at: 445 2351 2991 3617 445
Size: 1906 640 626 3202
Fragments arranged by size:
3202 1906 640 626
DraI TTT'AAA
Cuts at: 3373 3373
Size: 6374
DrdI GACnn_nn'nnGTC
Cuts at: 2463 4080 4938 2463
Size: 1617 858 3899
Fragments arranged by size:
3899 1617 858
EaeI y'GGCC_r
Cuts at: 1162 1458 1592 2405 3959 4133 4524 4551 5896
Size: 296 134 813 1554 174 391 27 1345
Cuts at: 5896 1162
Size: 1640
Fragments arranged by size:
1640 1554 1345 813 391 296 174 134
27
EagI C'GGCC_G
Cuts at: 1162 3959 1162
Size: 2797 3577
EarI CTCTTCn'nnn_
Cuts at: 1353 1544 2023 2165 2371 2447 2852 4397 4607
Size: 191 479 142 206 76 405 1545 210
Cuts at: 4607 1353
Size: 3120
Fragments arranged by size:
3120 1545 479 405 210 206 191 142
76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4582 4892 5038 6184 4582
Size: 310 146 1146 4772
Fragments arranged by size:
4772 1146 310 146
EcoICRI GAG'CTC
Cuts at: 816 1689 2253 3386 816
Size: 873 564 1133 3804
Fragments arranged by size:
3804 1133 873 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 480 719 1781 2128 2471 2487 3121 4199
Size: 218 239 1062 347 343 16 634 1078
Cuts at: 4199 4631 5378 5646 262
Size: 432 747 268 990
Fragments arranged by size:
1078 1062 990 747 634 432 347 343
268 239 218 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1802 2851 1802
Size: 1049 5325
EcoO109I rG'GnC_Cy
Cuts at: 237 3513 237
Size: 3276 3098
EcoRI G'AATT_C
Cuts at: 2536 3378 2536
Size: 842 5532
EcoRV GAT'ATC
Cuts at: 705 705
Size: 6374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 469 659 4393 469
Size: 190 3734 2450
Fragments arranged by size:
3734 2450 190
FspI TGC'GCA
Cuts at: 4155 4155
Size: 6374
HaeII r_GCGC'y
Cuts at: 202 2135 3265 3808 4056 5078 6057 202
Size: 1933 1130 543 248 1022 979 519
Fragments arranged by size:
1933 1130 1022 979 543 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 728 733 760 765 1597 1629 2283
Size: 32 575 5 27 5 832 32 654
Cuts at: 2283 2315 4216 4246 4248 4278 121
Size: 32 1901 30 2 30 2217
Fragments arranged by size:
2217 1901 832 654 575 32 32 32
30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 3413 3413
Size: 6374
KasI G'GCGC_C
Cuts at: 198 3804 4052 198
Size: 3606 248 2520
Fragments arranged by size:
3606 2520 248
KpnI G_GTAC'C
Cuts at: 3394 3394
Size: 6374
MluI A'CGCG_T
Cuts at: 5807 5807
Size: 6374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 581 2210 5045 5229 581
Size: 1629 2835 184 1726
Fragments arranged by size:
2835 1726 1629 184
MscI TGG'CCA
Cuts at: 1460 4135 1460
Size: 2675 3699
MslI CAynn'nnrTG
Cuts at: 277 1507 2705 3310 4363 4490 277
Size: 1230 1198 605 1053 127 2161
Fragments arranged by size:
2161 1230 1198 1053 605 127
MspA1I CmG'CkG
Cuts at: 259 2738 2869 3485 3799 4159 5172 5417 259
Size: 2479 131 616 314 360 1013 245 1216
Fragments arranged by size:
2479 1216 1013 616 360 314 245 131
NaeI GCC'GGC
Cuts at: 4555 4555
Size: 6374
NarI GG'CG_CC
Cuts at: 199 3805 4053 199
Size: 3606 248 2520
Fragments arranged by size:
3606 2520 248
NdeI CA'TA_TG
Cuts at: 3066 3066
Size: 6374
NgoMIV G'CCGG_C
Cuts at: 4553 4553
Size: 6374
NruI TCG'CGA
Cuts at: 1079 1079
Size: 6374
NsiI A_TGCA'T
Cuts at: 962 962
Size: 6374
NspI r_CATG'y
Cuts at: 438 3309 3427 3927 4834 438
Size: 2871 118 500 907 1978
Fragments arranged by size:
2871 1978 907 500 118
PasI CC'CwG_GG
Cuts at: 1789 1789
Size: 6374
PciI A'CATG_T
Cuts at: 3305 4830 3305
Size: 1525 4849
PflMI CCAn_nnn'nTGG
Cuts at: 3264 3358 3264
Size: 94 6280
PfoI T'CCnGG_A
Cuts at: 2846 2846
Size: 6374
PmeI GTTT'AAAC
Cuts at: 3373 3373
Size: 6374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2145 2177 5513 5545 2145
Size: 32 3336 32 2974
Fragments arranged by size:
3336 2974 32 32
PshAI GACnn'nnGTC
Cuts at: 1637 1637
Size: 6374
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1132 1164 1132
Size: 32 6342
PstI C_TGCA'G
Cuts at: 264 2751 3421 4106 264
Size: 2487 670 685 2532
Fragments arranged by size:
2532 2487 685 670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6374
PvuII CAG'CTG
Cuts at: 3799 4159 3799
Size: 360 6014
RsrII CG'GwC_CG
Cuts at: 4569 4569
Size: 6374
SacI G_AGCT'C
Cuts at: 818 1691 2255 3388 818
Size: 873 564 1133 3804
Fragments arranged by size:
3804 1133 873 564
SalI G'TCGA_C
Cuts at: 3411 3411
Size: 6374
SapI GCTCTTCn'nnn_
Cuts at: 1353 4397 4607 1353
Size: 3044 210 3120
Fragments arranged by size:
3120 3044 210
SbfI CC_TGCA'GG
Cuts at: 3421 3421
Size: 6374
ScaI AGT'ACT
Cuts at: 5891 5891
Size: 6374
SexAI A'CCwGG_T
Cuts at: 709 709
Size: 6374
SfcI C'TryA_G
Cuts at: 33 260 1830 2747 3417 4102 5095 5286 33
Size: 227 1570 917 670 685 993 191 1121
Fragments arranged by size:
1570 1121 993 917 685 670 227 191
SfoI GGC'GCC
Cuts at: 200 3806 4054 200
Size: 3606 248 2520
Fragments arranged by size:
3606 2520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6374
SmaI CCC'GGG
Cuts at: 3396 3396
Size: 6374
SmlI C'TyrA_G
Cuts at: 2127 2254 2279 3529 4444 4936 5198 2127
Size: 127 25 1250 915 492 262 3303
Fragments arranged by size:
3303 1250 915 492 262 127 25
SnaBI TAC'GTA
Cuts at: 569 569
Size: 6374
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6374
SphI G_CATG'C
Cuts at: 438 3427 3927 438
Size: 2989 500 2885
Fragments arranged by size:
2989 2885 500
StuI AGG'CCT
Cuts at: 1797 1797
Size: 6374
StyI C'CwwG_G
Cuts at: 218 1112 1310 1772 1803 2114 3508 3862 218
Size: 894 198 462 31 311 1394 354 2730
Fragments arranged by size:
2730 1394 894 462 354 311 198 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 389 1675 3957 4090 4549 4758 130
Size: 259 1286 2282 133 459 209 1746
Fragments arranged by size:
2282 1746 1286 459 259 209 133
TatI w'GTAC_w
Cuts at: 5889 5889
Size: 6374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1230 1262 1497 1529 5891 5923 1230
Size: 32 235 32 4362 32 1681
Fragments arranged by size:
4362 1681 235 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2598 4171 2598
Size: 1573 4801
XbaI T'CTAG_A
Cuts at: 3405 3405
Size: 6374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3869 3869
Size: 6374
XmaI C'CCGG_G
Cuts at: 3394 3394
Size: 6374
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI BaeI BglI BmgBI
BmtI BsiWI BsrGI BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI PacI PmlI PpuMI PsiI PspOMI PspXI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI