Restriction map for
pF1KA0984
(Circular) (Six-base) MAPSORT of: pF1KA0984.seq Check: 972 from: 1 to: 5054
pF1KA0984 5054 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 2092 2092
Size: 5054
Acc65I G'GTAC_C
Cuts at: 2070 2070
Size: 5054
AclI AA'CG_TT
Cuts at: 4894 4894
Size: 5054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 484 1216 1661 1992 2879 3311 3664 4326
Size: 55 732 445 331 887 432 353 662
Cuts at: 4326 429
Size: 1157
Fragments arranged by size:
1157 887 732 662 445 432 353 331
55
AflII C'TTAA_G
Cuts at: 1435 1435
Size: 5054
AflIII A'CryG_T
Cuts at: 2406 4206 4487 2406
Size: 1800 281 2973
Fragments arranged by size:
2973 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1505 1505
Size: 5054
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1672 1704 1672
Size: 32 5022
AlwNI CAG_nnn'CTG
Cuts at: 489 913 1077 2359 3797 489
Size: 424 164 1282 1438 1746
Fragments arranged by size:
1746 1438 1282 424 164
ApaLI G'TGCA_C
Cuts at: 1786 1826 1981 3892 1786
Size: 40 155 1911 2948
Fragments arranged by size:
2948 1911 155 40
ApoI r'AATT_y
Cuts at: 158 727 2058 4407 4663 158
Size: 569 1331 2349 256 549
Fragments arranged by size:
2349 1331 569 549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
AvaI C'yCGr_G
Cuts at: 2074 2074
Size: 5054
BamHI G'GATC_C
Cuts at: 2079 2079
Size: 5054
BanI G'GyrC_C
Cuts at: 746 2070 2484 2732 2767 746
Size: 1324 414 248 35 3033
Fragments arranged by size:
3033 1324 414 248 35
BanII G_rGCy'C
Cuts at: 542 1740 1781 2068 3098 542
Size: 1198 41 287 1030 2498
Fragments arranged by size:
2498 1198 1030 287 41
BbeI G_GCGC'C
Cuts at: 750 2488 2736 750
Size: 1738 248 3068
Fragments arranged by size:
3068 1738 248
BbsI GAAGACnn'nnnn_
Cuts at: 435 549 627 1436 435
Size: 114 78 809 4053
Fragments arranged by size:
4053 809 114 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 952 986 1192 1226 90
Size: 34 828 34 206 34 3918
Fragments arranged by size:
3918 828 206 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 486 2952 4008 5054 486
Size: 2466 1056 1046 486
Fragments arranged by size:
2466 1056 1046 486
BclI T'GATC_A
Cuts at: 2574 2574
Size: 5054
BglI GCCn_nnn'nGGC
Cuts at: 1643 1643
Size: 5054
BglII A'GATC_T
Cuts at: 620 1081 2569 4606 620
Size: 461 1488 2037 1068
Fragments arranged by size:
2037 1488 1068 461
BlpI GC'TnA_GC
Cuts at: 218 2166 218
Size: 1948 3106
Bme1580I G_kGCm'C
Cuts at: 1790 1830 1985 2679 2772 3896 1790
Size: 40 155 694 93 1124 2948
Fragments arranged by size:
2948 1124 694 155 93 40
BmrI ACTGGGnnnn_n'
Cuts at: 2266 2347 2682 4826 2266
Size: 81 335 2144 2494
Fragments arranged by size:
2494 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 5054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1295
Size: 5054
Bpu10I CC'TnA_GC
Cuts at: 1780 1780
Size: 5054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 3109 3823 4121 2230
Size: 879 714 298 3163
Fragments arranged by size:
3163 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 443 4758 443
Size: 4315 739
BsaAI yAC'GTr
Cuts at: 2407 2407
Size: 5054
BsaBI GATnn'nnATC
Cuts at: 6 2568 4605 6
Size: 2562 2037 455
Fragments arranged by size:
2562 2037 455
BsaHI Gr'CG_yC
Cuts at: 747 2485 2733 3435 4931 747
Size: 1738 248 702 1496 870
Fragments arranged by size:
1738 1496 870 702 248
BsaWI w'CCGG_w
Cuts at: 1677 2243 2764 3442 3853 4000 4274 1677
Size: 566 521 678 411 147 274 2457
Fragments arranged by size:
2457 678 566 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1672 1702 1672
Size: 30 5024
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1081 1081
Size: 5054
BseYI C'CCAG_C
Cuts at: 885 1575 1618 1903 2479 3237 3902 4310 885
Size: 690 43 285 576 758 665 408 1629
Fragments arranged by size:
1629 758 690 665 576 408 285 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 296
Size: 5054
BsiEI CG_ry'CG
Cuts at: 104 1664 2596 2642 3872 104
Size: 1560 932 46 1230 1286
Fragments arranged by size:
1560 1286 1230 932 46
BsiHKAI G_wGCw'C
Cuts at: 1781 1790 1830 1985 2068 2846 3036 3896 1781
Size: 9 40 155 83 778 190 860 2939
Fragments arranged by size:
2939 860 778 190 155 83 40 9
BsmI GAATG_Cn'
Cuts at: 1283 1377 1591 1283
Size: 94 214 4746
Fragments arranged by size:
4746 214 94
BsmBI CGTCTCn'nnnn_
Cuts at: 1298 1298
Size: 5054
Bsp1286I G_dGCh'C
Cuts at: 542 1740 1781 1790 1830 1985 2068 2679 2772
Size: 1198 41 9 40 155 83 611 93
Cuts at: 2772 2846 3036 3098 3896 542
Size: 74 190 62 798 1700
Fragments arranged by size:
1700 1198 798 611 190 155 93 83
74 62 41 40 9
BspEI T'CCGG_A
Cuts at: 1677 1677
Size: 5054
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1227 2104 2318 2620 3001 1227
Size: 877 214 302 381 3280
Fragments arranged by size:
3280 877 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3346 3400 4884 5049 50
Size: 3296 54 1484 165 55
Fragments arranged by size:
3296 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 345 1091 2966 345
Size: 746 1875 2433
Fragments arranged by size:
2433 1875 746
BsrFI r'CCGG_y
Cuts at: 1683 3052 3233 1683
Size: 1369 181 3504
Fragments arranged by size:
3504 1369 181
BsrGI T'GTAC_A
Cuts at: 1250 1606 1250
Size: 356 4698
BssSI C'ACGA_G
Cuts at: 3325 4033 3325
Size: 708 4346
BstAPI GCAn_nnn'nTGC
Cuts at: 2431 2431
Size: 5054
BstBI TT'CG_AA
Cuts at: 3415 3415
Size: 5054
BstXI CCAn_nnnn'nTGG
Cuts at: 1390 1575 1944 1390
Size: 185 369 4500
Fragments arranged by size:
4500 369 185
BstYI r'GATC_y
Cuts at: 1 620 1081 2079 2546 2569 2904 3150 3456
Size: 619 461 998 467 23 335 246 306
Cuts at: 3456 3468 3554 4606 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 998 619 467 461 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 661 661
Size: 5054
BtgI C'CryG_G
Cuts at: 152 740 815 1456 152
Size: 588 75 641 3750
Fragments arranged by size:
3750 641 588 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3004 3185 3348 3004
Size: 181 163 4710
Fragments arranged by size:
4710 181 163
BtsI GCAGTG_nn'
Cuts at: 574 992 1806 2297 574
Size: 418 814 491 3331
Fragments arranged by size:
3331 814 491 418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 504 539 504
Size: 35 5019
DraI TTT'AAA
Cuts at: 617 2053 617
Size: 1436 3618
DrdI GACnn_nn'nnGTC
Cuts at: 2760 4104 2760
Size: 1344 3710
EaeI y'GGCC_r
Cuts at: 207 2639 2813 3204 3231 4576 207
Size: 2432 174 391 27 1345 685
Fragments arranged by size:
2432 1345 685 391 174 27
EagI C'GGCC_G
Cuts at: 2639 2639
Size: 5054
EarI CTCTTCn'nnn_
Cuts at: 503 941 3077 3287 503
Size: 438 2136 210 2270
Fragments arranged by size:
2270 2136 438 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3262 4004 4150 4864 3262
Size: 742 146 714 3452
Fragments arranged by size:
3452 742 714 146
EcoICRI GAG'CTC
Cuts at: 1779 2066 1779
Size: 287 4767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 484 1216 1295 1661 1992 2879 3311 3664
Size: 55 732 79 366 331 887 432 353
Cuts at: 3664 4326 429
Size: 662 1157
Fragments arranged by size:
1157 887 732 662 432 366 353 331
79 55
EcoNI CCTnn'n_nnAGG
Cuts at: 754 754
Size: 5054
EcoO109I rG'GnC_Cy
Cuts at: 174 2193 174
Size: 2019 3035
EcoRI G'AATT_C
Cuts at: 158 727 2058 158
Size: 569 1331 3154
Fragments arranged by size:
3154 1331 569
EcoRV GAT'ATC
Cuts at: 1185 1185
Size: 5054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 5054
FspI TGC'GCA
Cuts at: 2835 2835
Size: 5054
HaeII r_GCGC'y
Cuts at: 750 2488 2736 3966 4737 750
Size: 1738 248 1230 771 1067
Fragments arranged by size:
1738 1230 1067 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 1491 1523 1657 1689 2896 2926 2928
Size: 32 1326 32 134 32 1207 30 2
Cuts at: 2928 2958 133
Size: 30 2229
Fragments arranged by size:
2229 1326 1207 134 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2093 2093
Size: 5054
KasI G'GCGC_C
Cuts at: 746 2484 2732 746
Size: 1738 248 3068
Fragments arranged by size:
3068 1738 248
KpnI G_GTAC'C
Cuts at: 2074 2074
Size: 5054
MfeI C'AATT_G
Cuts at: 838 838
Size: 5054
MluI A'CGCG_T
Cuts at: 4487 4487
Size: 5054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3813 3997 3813
Size: 184 4870
MscI TGG'CCA
Cuts at: 209 2815 209
Size: 2606 2448
MslI CAynn'nnrTG
Cuts at: 3043 3170 3043
Size: 127 4927
MspA1I CmG'CkG
Cuts at: 1618 1768 1803 2165 2479 2839 3623 3868 1618
Size: 150 35 362 314 360 784 245 2804
Fragments arranged by size:
2804 784 362 360 314 245 150 35
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 5054
NarI GG'CG_CC
Cuts at: 747 2485 2733 747
Size: 1738 248 3068
Fragments arranged by size:
3068 1738 248
NcoI C'CATG_G
Cuts at: 152 740 815 152
Size: 588 75 4391
Fragments arranged by size:
4391 588 75
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 5054
NspI r_CATG'y
Cuts at: 596 1494 2107 2607 4210 596
Size: 898 613 500 1603 1440
Fragments arranged by size:
1603 1440 898 613 500
PciI A'CATG_T
Cuts at: 4206 4206
Size: 5054
PfoI T'CCnGG_A
Cuts at: 1107 1107
Size: 5054
PmeI GTTT'AAAC
Cuts at: 2053 2053
Size: 5054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1672 1704 3500 3532 1672
Size: 32 1796 32 3194
Fragments arranged by size:
3194 1796 32 32
PpuMI rG'GwC_Cy
Cuts at: 174 174
Size: 5054
PsiI TTA'TAA
Cuts at: 1318 1318
Size: 5054
PstI C_TGCA'G
Cuts at: 1034 1719 1767 2101 2786 1034
Size: 685 48 334 685 3302
Fragments arranged by size:
3302 685 685 334 48
PvuI CG_AT'CG
Cuts at: 104 1664 104
Size: 1560 3494
PvuII CAG'CTG
Cuts at: 1618 1768 2479 2839 1618
Size: 150 711 360 3833
Fragments arranged by size:
3833 711 360 150
RsrII CG'GwC_CG
Cuts at: 3249 3249
Size: 5054
SacI G_AGCT'C
Cuts at: 1781 2068 1781
Size: 287 4767
SalI G'TCGA_C
Cuts at: 2091 2091
Size: 5054
SapI GCTCTTCn'nnn_
Cuts at: 3077 3287 3077
Size: 210 4844
SbfI CC_TGCA'GG
Cuts at: 2101 2101
Size: 5054
ScaI AGT'ACT
Cuts at: 1207 4571 1207
Size: 3364 1690
SfcI C'TryA_G
Cuts at: 33 192 732 1030 1715 1763 1961 2097 2782
Size: 159 540 298 685 48 198 136 685
Cuts at: 2782 3750 3941 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 685 540 298 198 191
159 136 48
SfoI GGC'GCC
Cuts at: 748 2486 2734 748
Size: 1738 248 3068
Fragments arranged by size:
3068 1738 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
SmaI CCC'GGG
Cuts at: 2076 2076
Size: 5054
SmlI C'TyrA_G
Cuts at: 1435 2209 3124 3838 4100 1435
Size: 774 915 714 262 2389
Fragments arranged by size:
2389 915 774 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5054
SphI G_CATG'C
Cuts at: 2107 2607 2107
Size: 500 4554
StyI C'CwwG_G
Cuts at: 152 291 740 815 1495 1863 2188 2542 152
Size: 139 449 75 680 368 325 354 2664
Fragments arranged by size:
2664 680 449 368 354 325 139 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1805 2637 2770 3229 3438 1805
Size: 832 133 459 209 3421
Fragments arranged by size:
3421 832 459 209 133
TatI w'GTAC_w
Cuts at: 1205 1250 1606 4569 1205
Size: 45 356 2963 1690
Fragments arranged by size:
2963 1690 356 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4571 4603 4571
Size: 32 5022
Tth111I GACn'n_nGTC
Cuts at: 2851 2851
Size: 5054
XbaI T'CTAG_A
Cuts at: 2085 2085
Size: 5054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2549 2549
Size: 5054
XmaI C'CCGG_G
Cuts at: 2074 2074
Size: 5054
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI BssHII
BstEII BstZ17I ClaI DraIII FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI