Restriction map for
pF1KA0983
(Circular) (Six-base) MAPSORT of: pF1KA0983.seq Check: 6331 from: 1 to: 5162
pF1KA0983 5162 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 289 905 1270 289
Size: 616 365 4181
Fragments arranged by size:
4181 616 365
AccI GT'mk_AC
Cuts at: 2200 2200
Size: 5162
Acc65I G'GTAC_C
Cuts at: 2178 2178
Size: 5162
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 343 1150 1183 1564 2076 2130 2987 3419
Size: 152 807 33 381 512 54 857 432
Cuts at: 3419 3772 4434 191
Size: 353 662 919
Fragments arranged by size:
919 857 807 662 512 432 381 353
152 54 33
AflIII A'CryG_T
Cuts at: 2514 4314 4595 2514
Size: 1800 281 3081
Fragments arranged by size:
3081 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1290 1290
Size: 5162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1141 1141
Size: 5162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 356 388 356
Size: 32 5130
AlwNI CAG_nnn'CTG
Cuts at: 300 504 1446 1992 2467 3905 300
Size: 204 942 546 475 1438 1557
Fragments arranged by size:
1557 1438 942 546 475 204
ApaI G_GGCC'C
Cuts at: 2054 2054
Size: 5162
ApaLI G'TGCA_C
Cuts at: 4000 4000
Size: 5162
ApoI r'AATT_y
Cuts at: 2059 2166 4515 4771 2059
Size: 107 2349 256 2450
Fragments arranged by size:
2450 2349 256 107
AseI AT'TA_AT
Cuts at: 20 20
Size: 5162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 2182 2182
Size: 5162
BamHI G'GATC_C
Cuts at: 371 2187 371
Size: 1816 3346
BanI G'GyrC_C
Cuts at: 1136 2178 2592 2840 2875 1136
Size: 1042 414 248 35 3423
Fragments arranged by size:
3423 1042 414 248 35
BanII G_rGCy'C
Cuts at: 165 2054 2176 3206 165
Size: 1889 122 1030 2121
Fragments arranged by size:
2121 1889 1030 122
BbeI G_GCGC'C
Cuts at: 2596 2844 2596
Size: 248 4914
BbsI GAAGACnn'nnnn_
Cuts at: 1552 1552
Size: 5162
BciVI GTATCCnnnnn_n'
Cuts at: 1331 3060 4116 5162 1331
Size: 1729 1056 1046 1331
Fragments arranged by size:
1729 1331 1056 1046
BclI T'GATC_A
Cuts at: 1262 2682 1262
Size: 1420 3742
BglII A'GATC_T
Cuts at: 2677 4714 2677
Size: 2037 3125
BlpI GC'TnA_GC
Cuts at: 464 1507 2274 464
Size: 1043 767 3352
Fragments arranged by size:
3352 1043 767
Bme1580I G_kGCm'C
Cuts at: 1786 2054 2787 2880 4004 1786
Size: 268 733 93 1124 2944
Fragments arranged by size:
2944 1124 733 268 93
BmgBI CAC'GTC
Cuts at: 676 676
Size: 5162
BmrI ACTGGGnnnn_n'
Cuts at: 693 1102 2374 2455 2790 4934 693
Size: 409 1272 81 335 2144 921
Fragments arranged by size:
2144 1272 921 409 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1062 3529 1062
Size: 2467 2695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 712 778 1001 2038 481
Size: 231 66 223 1037 3605
Fragments arranged by size:
3605 1037 231 223 66
Bpu10I CC'TnA_GC
Cuts at: 784 784
Size: 5162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1432 2338 3217 3931 4229 739
Size: 693 906 879 714 298 1672
Fragments arranged by size:
1672 906 879 714 693 298
BsaI GGTCTCn'nnnn_
Cuts at: 644 1077 1519 2014 4866 644
Size: 433 442 495 2852 940
Fragments arranged by size:
2852 940 495 442 433
BsaAI yAC'GTr
Cuts at: 2515 2515
Size: 5162
BsaBI GATnn'nnATC
Cuts at: 6 482 2676 4713 6
Size: 476 2194 2037 455
Fragments arranged by size:
2194 2037 476 455
BsaHI Gr'CG_yC
Cuts at: 2593 2841 3543 5039 2593
Size: 248 702 1496 2716
Fragments arranged by size:
2716 1496 702 248
BsaWI w'CCGG_w
Cuts at: 831 2351 2872 3550 3961 4108 4382 831
Size: 1520 521 678 411 147 274 1611
Fragments arranged by size:
1611 1520 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 832 862 2062 2092 544
Size: 30 258 30 1200 30 3614
Fragments arranged by size:
3614 1200 258 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 610 1072 1075 1818 238
Size: 372 462 3 743 3582
Fragments arranged by size:
3582 743 462 372 3
BseYI C'CCAG_C
Cuts at: 165 1446 1478 1741 1957 2587 3345 4010 4418
Size: 1281 32 263 216 630 758 665 408
Cuts at: 4418 165
Size: 909
Fragments arranged by size:
1281 909 758 665 630 408 263 216
32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1298 1459 1576 1690 856
Size: 442 161 117 114 4328
Fragments arranged by size:
4328 442 161 117 114
BsiEI CG_ry'CG
Cuts at: 104 2704 2750 3980 104
Size: 2600 46 1230 1286
Fragments arranged by size:
2600 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2176 2954 3144 4004 2176
Size: 778 190 860 3334
Fragments arranged by size:
3334 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 165 1786 2054 2176 2787 2880 2954 3144 3206
Size: 1621 268 122 611 93 74 190 62
Cuts at: 3206 4004 165
Size: 798 1323
Fragments arranged by size:
1621 1323 798 611 268 190 122 93
74 62
BspHI T'CATG_A
Cuts at: 392 1208 1223 3594 392
Size: 816 15 2371 1960
Fragments arranged by size:
2371 1960 816 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 289 905 1270 2212 2426 2728 3109 289
Size: 616 365 942 214 302 381 2342
Fragments arranged by size:
2342 942 616 381 365 302 214
BsrBI CCG'CTC
Cuts at: 50 3454 3508 4992 5157 50
Size: 3404 54 1484 165 55
Fragments arranged by size:
3404 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3074 3074
Size: 5162
BsrFI r'CCGG_y
Cuts at: 3160 3341 3160
Size: 181 4981
BssSI C'ACGA_G
Cuts at: 457 3433 4141 457
Size: 2976 708 1478
Fragments arranged by size:
2976 1478 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2539 2539
Size: 5162
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstEII G'GTnAC_C
Cuts at: 1420 1420
Size: 5162
BstXI CCAn_nnnn'nTGG
Cuts at: 549 1521 549
Size: 972 4190
BstYI r'GATC_y
Cuts at: 1 237 311 371 2187 2654 2677 3012 3258
Size: 236 74 60 1816 467 23 335 246
Cuts at: 3258 3564 3576 3662 4714 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1816 1052 467 449 335 306 246 236
86 74 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 2135 2135
Size: 5162
BtgI C'CryG_G
Cuts at: 644 1253 1907 644
Size: 609 654 3899
Fragments arranged by size:
3899 654 609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 170 2137 3112 3293 3456 170
Size: 1967 975 181 163 1876
Fragments arranged by size:
1967 1876 975 181 163
BtsI GCAGTG_nn'
Cuts at: 932 990 2405 932
Size: 58 1415 3689
Fragments arranged by size:
3689 1415 58
ClaI AT'CG_AT
Cuts at: 186 186
Size: 5162
DraI TTT'AAA
Cuts at: 2121 2161 2121
Size: 40 5122
DrdI GACnn_nn'nnGTC
Cuts at: 2868 4212 2868
Size: 1344 3818
EaeI y'GGCC_r
Cuts at: 2747 2921 3312 3339 4684 2747
Size: 174 391 27 1345 3225
Fragments arranged by size:
3225 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2747 2747
Size: 5162
EarI CTCTTCn'nnn_
Cuts at: 319 438 1126 1642 2095 3185 3395 319
Size: 119 688 516 453 1090 210 2086
Fragments arranged by size:
2086 1090 688 516 453 210 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3370 4112 4258 4972 3370
Size: 742 146 714 3560
Fragments arranged by size:
3560 742 714 146
EcoICRI GAG'CTC
Cuts at: 2174 2174
Size: 5162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 343 481 712 778 1001 1150 1183 1564
Size: 152 138 231 66 223 149 33 381
Cuts at: 1564 2038 2076 2130 2987 3419 3772 4434 191
Size: 474 38 54 857 432 353 662 919
Fragments arranged by size:
919 857 662 474 432 381 353 231
223 152 149 138 66 54 38 33
EcoNI CCTnn'n_nnAGG
Cuts at: 529 529
Size: 5162
EcoO109I rG'GnC_Cy
Cuts at: 246 2050 2098 2301 246
Size: 1804 48 203 3107
Fragments arranged by size:
3107 1804 203 48
EcoRI G'AATT_C
Cuts at: 2166 2166
Size: 5162
EcoRV GAT'ATC
Cuts at: 624 624
Size: 5162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 5162
FspI TGC'GCA
Cuts at: 2943 2943
Size: 5162
HaeII r_GCGC'y
Cuts at: 1080 1514 1771 2596 2844 4074 4845 1080
Size: 434 257 825 248 1230 771 1397
Fragments arranged by size:
1397 1230 825 771 434 257 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 718 750 1276 1308 3004 3034 3036
Size: 32 142 32 526 32 1696 30 2
Cuts at: 3036 3066 544
Size: 30 2640
Fragments arranged by size:
2640 1696 526 142 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1973 2201 1973
Size: 228 4934
HindIII A'AGCT_T
Cuts at: 406 406
Size: 5162
KasI G'GCGC_C
Cuts at: 2592 2840 2592
Size: 248 4914
KpnI G_GTAC'C
Cuts at: 2182 2182
Size: 5162
MfeI C'AATT_G
Cuts at: 1679 1679
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1085 3921 4105 1085
Size: 2836 184 2142
Fragments arranged by size:
2836 2142 184
MscI TGG'CCA
Cuts at: 2923 2923
Size: 5162
MslI CAynn'nnrTG
Cuts at: 3151 3278 3151
Size: 127 5035
MspA1I CmG'CkG
Cuts at: 169 699 1804 1968 1983 1989 2273 2587 2947
Size: 530 1105 164 15 6 284 314 360
Cuts at: 2947 3731 3976 169
Size: 784 245 1355
Fragments arranged by size:
1355 1105 784 530 360 314 284 245
164 15 6
NaeI GCC'GGC
Cuts at: 3343 3343
Size: 5162
NarI GG'CG_CC
Cuts at: 2593 2841 2593
Size: 248 4914
NcoI C'CATG_G
Cuts at: 644 1253 1907 644
Size: 609 654 3899
Fragments arranged by size:
3899 654 609
NgoMIV G'CCGG_C
Cuts at: 3341 3341
Size: 5162
NspI r_CATG'y
Cuts at: 2215 2715 4318 2215
Size: 500 1603 3059
Fragments arranged by size:
3059 1603 500
PciI A'CATG_T
Cuts at: 4314 4314
Size: 5162
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 5130
PpuMI rG'GwC_Cy
Cuts at: 246 2098 246
Size: 1852 3310
PsiI TTA'TAA
Cuts at: 605 605
Size: 5162
PspOMI G'GGCC_C
Cuts at: 2050 2050
Size: 5162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1807 1839 1807
Size: 32 5130
PstI C_TGCA'G
Cuts at: 494 1799 1988 2209 2894 494
Size: 1305 189 221 685 2762
Fragments arranged by size:
2762 1305 685 221 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5162
PvuII CAG'CTG
Cuts at: 169 1968 1983 2587 2947 169
Size: 1799 15 604 360 2384
Fragments arranged by size:
2384 1799 604 360 15
RsrII CG'GwC_CG
Cuts at: 3357 3357
Size: 5162
SacI G_AGCT'C
Cuts at: 2176 2176
Size: 5162
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SanDI GG'GwC_CC
Cuts at: 246 246
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 319 2095 3185 3395 319
Size: 1776 1090 210 2086
Fragments arranged by size:
2086 1776 1090 210
SbfI CC_TGCA'GG
Cuts at: 2209 2209
Size: 5162
ScaI AGT'ACT
Cuts at: 4679 4679
Size: 5162
SfcI C'TryA_G
Cuts at: 33 216 292 490 526 1795 1984 2205 2890
Size: 183 76 198 36 1269 189 221 685
Cuts at: 2890 3858 4049 33
Size: 968 191 1146
Fragments arranged by size:
1269 1146 968 685 221 198 191 189
183 76 36
SfoI GGC'GCC
Cuts at: 2594 2842 2594
Size: 248 4914
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 2184 2184
Size: 5162
SmlI C'TyrA_G
Cuts at: 754 1411 2317 3232 3946 4208 754
Size: 657 906 915 714 262 1708
Fragments arranged by size:
1708 915 906 714 657 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5162
SphI G_CATG'C
Cuts at: 2215 2715 2215
Size: 500 4662
StuI AGG'CCT
Cuts at: 949 949
Size: 5162
StyI C'CwwG_G
Cuts at: 644 998 1253 1646 1907 1951 2054 2296 2650
Size: 354 255 393 261 44 103 242 354
Cuts at: 2650 644
Size: 3156
Fragments arranged by size:
3156 393 354 354 261 255 242 103
44
SwaI ATTT'AAAT
Cuts at: 2121 2121
Size: 5162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1154 1425 1438 1662 2038 2745 2878 3337 3546
Size: 271 13 224 376 707 133 459 209
Cuts at: 3546 1154
Size: 2770
Fragments arranged by size:
2770 707 459 376 271 224 209 133
13
TatI w'GTAC_w
Cuts at: 275 1407 4677 275
Size: 1132 3270 760
Fragments arranged by size:
3270 1132 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1829 1861 2062 2094 4679 4711 1829
Size: 32 201 32 2585 32 2280
Fragments arranged by size:
2585 2280 201 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1303 2959 1303
Size: 1656 3506
XbaI T'CTAG_A
Cuts at: 2193 2193
Size: 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1722 2657 1722
Size: 935 4227
XmaI C'CCGG_G
Cuts at: 2182 2182
Size: 5162
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstZ17I CspCI DraIII FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI XhoI XmnI ZraI