Restriction map for
pF1KA0968
(Circular) (Six-base) MAPSORT of: pF1KA0968.seq Check: 4714 from: 1 to: 4547
pF1KA0968 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 130 130
Size: 4547
AccI GT'mk_AC
Cuts at: 1585 1585
Size: 4547
Acc65I G'GTAC_C
Cuts at: 734 1563 734
Size: 829 3718
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 535 997 1024 2372 2804 3551 3819 328
Size: 207 462 27 1348 432 747 268 1056
Fragments arranged by size:
1348 1056 747 462 432 268 207 27
AflII C'TTAA_G
Cuts at: 904 904
Size: 4547
AflIII A'CryG_T
Cuts at: 1899 3003 3980 1899
Size: 1104 977 2466
Fragments arranged by size:
2466 1104 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AleI CACnn'nnGTG
Cuts at: 1138 1138
Size: 4547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1351 1351
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 276 449 465 1419 1852 3419 276
Size: 173 16 954 433 1567 1404
Fragments arranged by size:
1567 1404 954 433 173 16
ApaLI G'TGCA_C
Cuts at: 502 1336 3317 502
Size: 834 1981 1732
Fragments arranged by size:
1981 1732 834
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 416 1567 416
Size: 1151 3396
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 553 734 790 1472 1521 1563 1977 2225 2260
Size: 181 56 682 49 42 414 248 35
Cuts at: 2260 553
Size: 2840
Fragments arranged by size:
2840 682 414 248 181 56 49 42
35
BanII G_rGCy'C
Cuts at: 1561 2591 1561
Size: 1030 3517
BbeI G_GCGC'C
Cuts at: 794 1525 1981 2229 794
Size: 731 456 248 3112
Fragments arranged by size:
3112 731 456 248
BbsI GAAGACnn'nnnn_
Cuts at: 1136 1136
Size: 4547
BciVI GTATCCnnnnn_n'
Cuts at: 463 2445 3206 4547 463
Size: 1982 761 1341 463
Fragments arranged by size:
1982 1341 761 463
BclI T'GATC_A
Cuts at: 848 2067 848
Size: 1219 3328
BglI GCCn_nnn'nGGC
Cuts at: 1484 1484
Size: 4547
BglII A'GATC_T
Cuts at: 1513 2062 4099 1513
Size: 549 2037 1961
Fragments arranged by size:
2037 1961 549
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 506 1340 2172 2265 3321 506
Size: 834 832 93 1056 1732
Fragments arranged by size:
1732 1056 834 832 93
BmrI ACTGGGnnnn_n'
Cuts at: 1443 1759 1840 2175 4319 1443
Size: 316 81 335 2144 1671
Fragments arranged by size:
2144 1671 335 316 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 2914 1033
Size: 1881 2666
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 487 298
Size: 189 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1723 2602 3094 3392 532
Size: 1191 879 492 298 1687
Fragments arranged by size:
1687 1191 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 257 955 1453 4251 257
Size: 698 498 2798 553
Fragments arranged by size:
2798 698 553 498
BsaAI yAC'GTr
Cuts at: 1900 1900
Size: 4547
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 791 1522 1978 2226 2928 4424 791
Size: 731 456 248 702 1496 914
Fragments arranged by size:
1496 914 731 702 456 248
BsaWI w'CCGG_w
Cuts at: 1048 1736 2257 2935 3209 3356 3767 1048
Size: 688 521 678 274 147 411 1828
Fragments arranged by size:
1828 688 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 1471 364
Size: 1107 3440
BseYI C'CCAG_C
Cuts at: 1972 2730 3307 3803 1972
Size: 758 577 496 2716
Fragments arranged by size:
2716 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 463 108
Size: 355 4192
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 506 1108 1340 1561 2339 2529 3321 506
Size: 602 232 221 778 190 792 1732
Fragments arranged by size:
1732 792 778 602 232 221 190
BsiWI C'GTAC_G
Cuts at: 674 674
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 506 1108 1340 1561 2172 2265 2339 2529 2591
Size: 602 232 221 611 93 74 190 62
Cuts at: 2591 3321 506
Size: 730 1732
Fragments arranged by size:
1732 730 611 602 232 221 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1048 3767 1048
Size: 2719 1828
BspHI T'CATG_A
Cuts at: 3723 3723
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 130 1597 1811 2113 2494 130
Size: 1467 214 302 381 2183
Fragments arranged by size:
2183 1467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 929 2839 2893 4377 4542 50
Size: 879 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 879 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1200 2459 1200
Size: 1259 3288
BsrFI r'CCGG_y
Cuts at: 787 2545 2726 2935 787
Size: 1758 181 209 2399
Fragments arranged by size:
2399 1758 209 181
BsrGI T'GTAC_A
Cuts at: 716 716
Size: 4547
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 1247 2908 1247
Size: 1661 2886
BstXI CCAn_nnnn'nTGG
Cuts at: 594 1029 1516 594
Size: 435 487 3625
Fragments arranged by size:
3625 487 435
BstYI r'GATC_y
Cuts at: 1 461 843 917 1513 1572 2039 2062 2397
Size: 460 382 74 596 59 467 23 335
Cuts at: 2397 2643 3655 3741 3753 4099 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 596 467 460 449 382 346 335
246 86 74 59 23 12
Bsu36I CC'TnA_GG
Cuts at: 1253 1253
Size: 4547
BtgI C'CryG_G
Cuts at: 107 935 965 107
Size: 828 30 3689
Fragments arranged by size:
3689 828 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 791 1094 1377 2497 2678 2841 791
Size: 303 283 1120 181 163 2497
Fragments arranged by size:
2497 1120 303 283 181 163
BtsI GCAGTG_nn'
Cuts at: 480 566 1790 480
Size: 86 1224 3237
Fragments arranged by size:
3237 1224 86
ClaI AT'CG_AT
Cuts at: 1293 1293
Size: 4547
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 110 213 584 1031 2132 2306 2697 2724 4069
Size: 103 371 447 1101 174 391 27 1345
Cuts at: 4069 110
Size: 588
Fragments arranged by size:
1345 1101 588 447 391 371 174 103
27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 134 157 1051 1069 2570 2780 134
Size: 23 894 18 1501 210 1901
Fragments arranged by size:
1901 1501 894 210 23 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 451 2755 3065 3211 4357 451
Size: 2304 310 146 1146 641
Fragments arranged by size:
2304 1146 641 310 146
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 328 487 535 997 1024 2372 2804 3551
Size: 30 159 48 462 27 1348 432 747
Cuts at: 3551 3819 298
Size: 268 1026
Fragments arranged by size:
1348 1026 747 462 432 268 159 48
30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1273 1273
Size: 4547
EcoO109I rG'GnC_Cy
Cuts at: 510 669 1257 1278 1686 510
Size: 159 588 21 408 3371
Fragments arranged by size:
3371 588 408 159 21
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
EcoRV GAT'ATC
Cuts at: 408 643 408
Size: 235 4312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 192 2566 192
Size: 2374 2173
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 794 1525 1981 2229 3251 4230 794
Size: 731 456 248 1022 979 1111
Fragments arranged by size:
1111 1022 979 731 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 790 822 1192 1224 2389 2419 2421
Size: 32 430 32 370 32 1165 30 2
Cuts at: 2421 2451 328
Size: 30 2424
Fragments arranged by size:
2424 1165 430 370 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
KasI G'GCGC_C
Cuts at: 790 1521 1977 2225 790
Size: 731 456 248 3112
Fragments arranged by size:
3112 731 456 248
KpnI G_GTAC'C
Cuts at: 738 1567 738
Size: 829 3718
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 351 1433 3218 3402 351
Size: 1082 1785 184 1496
Fragments arranged by size:
1785 1496 1082 184
MscI TGG'CCA
Cuts at: 112 215 586 1033 2308 112
Size: 103 371 447 1275 2351
Fragments arranged by size:
2351 1275 447 371 103
MslI CAynn'nnrTG
Cuts at: 592 1024 1138 1219 2536 2663 592
Size: 432 114 81 1317 127 2476
Fragments arranged by size:
2476 1317 432 127 114 81
MspA1I CmG'CkG
Cuts at: 892 1176 1658 1972 2332 3345 3590 892
Size: 284 482 314 360 1013 245 1849
Fragments arranged by size:
1849 1013 482 360 314 284 245
NaeI GCC'GGC
Cuts at: 789 2728 789
Size: 1939 2608
NarI GG'CG_CC
Cuts at: 791 1522 1978 2226 791
Size: 731 456 248 3112
Fragments arranged by size:
3112 731 456 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 787 2726 787
Size: 1939 2608
NspI r_CATG'y
Cuts at: 951 1600 2100 3007 951
Size: 649 500 907 2491
Fragments arranged by size:
2491 907 649 500
PasI CC'CwG_GG
Cuts at: 1260 1260
Size: 4547
PciI A'CATG_T
Cuts at: 3003 3003
Size: 4547
PflMI CCAn_nnn'nTGG
Cuts at: 492 1371 492
Size: 879 3668
PfoI T'CCnGG_A
Cuts at: 464 636 1318 464
Size: 172 682 3693
Fragments arranged by size:
3693 682 172
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 510 669 510
Size: 159 4388
PshAI GACnn'nnGTC
Cuts at: 1465 1465
Size: 4547
PsiI TTA'TAA
Cuts at: 1159 1159
Size: 4547
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 727 759 727
Size: 32 4515
PstI C_TGCA'G
Cuts at: 1594 2279 1594
Size: 685 3862
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 892 1176 1972 2332 892
Size: 284 796 360 3107
Fragments arranged by size:
3107 796 360 284
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 157 1069 2570 2780 157
Size: 912 1501 210 1924
Fragments arranged by size:
1924 1501 912 210
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SexAI A'CCwGG_T
Cuts at: 377 1268 377
Size: 891 3656
SfcI C'TryA_G
Cuts at: 33 1590 2275 3268 3459 33
Size: 1557 685 993 191 1121
Fragments arranged by size:
1557 1121 993 685 191
SfoI GGC'GCC
Cuts at: 792 1523 1979 2227 792
Size: 731 456 248 3112
Fragments arranged by size:
3112 731 456 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 418 1569 418
Size: 1151 3396
SmlI C'TyrA_G
Cuts at: 547 904 1702 2617 3109 3371 547
Size: 357 798 915 492 262 1723
Fragments arranged by size:
1723 915 798 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 951 1600 2100 951
Size: 649 500 3398
Fragments arranged by size:
3398 649 500
StyI C'CwwG_G
Cuts at: 107 839 1681 2035 107
Size: 732 842 354 2619
Fragments arranged by size:
2619 842 732 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2130 2263 2722 2931 2130
Size: 133 459 209 3746
Fragments arranged by size:
3746 459 209 133
TatI w'GTAC_w
Cuts at: 716 4062 716
Size: 3346 1201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4515
Tth111I GACn'n_nGTC
Cuts at: 379 823 2344 379
Size: 444 1521 2582
Fragments arranged by size:
2582 1521 444
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 812 2042 812
Size: 1230 3317
XmaI C'CCGG_G
Cuts at: 416 1567 416
Size: 1151 3396
XmnI GAAnn'nnTTC
Cuts at: 982 982
Size: 4547
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsmI
BsmBI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI