Restriction map for
pF1KA0966
(Circular) (Six-base) MAPSORT of: pF1KA0966.seq Check: 5834 from: 1 to: 6509
pF1KA0966 6509 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1241 3547 1241
Size: 2306 4203
Acc65I G'GTAC_C
Cuts at: 3525 3525
Size: 6509
AclI AA'CG_TT
Cuts at: 6349 6349
Size: 6509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 628 1303 1693 2260 2273 2648 2846 3269
Size: 108 675 390 567 13 375 198 423
Cuts at: 3269 4334 4766 5513 5781 520
Size: 1065 432 747 268 1248
Fragments arranged by size:
1248 1065 747 675 567 432 423 390
375 268 198 108 13
AflIII A'CryG_T
Cuts at: 2657 3861 4965 5942 2657
Size: 1204 1104 977 3224
Fragments arranged by size:
3224 1204 1104 977
AgeI A'CCGG_T
Cuts at: 4897 4897
Size: 6509
AleI CACnn'nnGTG
Cuts at: 1504 2620 1504
Size: 1116 5393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2203 3224 2203
Size: 1021 5488
AlwNI CAG_nnn'CTG
Cuts at: 1548 2647 3427 3814 5381 1548
Size: 1099 780 387 1567 2676
Fragments arranged by size:
2676 1567 1099 780 387
ApaI G_GGCC'C
Cuts at: 1974 1974
Size: 6509
ApaLI G'TGCA_C
Cuts at: 5279 5279
Size: 6509
ApoI r'AATT_y
Cuts at: 269 2430 2446 2692 2703 3513 5862 6118 269
Size: 2161 16 246 11 810 2349 256 660
Fragments arranged by size:
2349 2161 810 660 256 246 16 11
AseI AT'TA_AT
Cuts at: 20 3437 20
Size: 3417 3092
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
AvaI C'yCGr_G
Cuts at: 3529 3529
Size: 6509
BamHI G'GATC_C
Cuts at: 3534 3534
Size: 6509
BanI G'GyrC_C
Cuts at: 3525 3939 4187 4222 3525
Size: 414 248 35 5812
Fragments arranged by size:
5812 414 248 35
BanII G_rGCy'C
Cuts at: 116 425 1974 3523 4553 116
Size: 309 1549 1549 1030 2072
Fragments arranged by size:
2072 1549 1549 1030 309
BbeI G_GCGC'C
Cuts at: 3943 4191 3943
Size: 248 6261
BbsI GAAGACnn'nnnn_
Cuts at: 508 2384 2654 2726 508
Size: 1876 270 72 4291
Fragments arranged by size:
4291 1876 270 72
BbvCI CC'TCA_GC
Cuts at: 2796 2796
Size: 6509
BciVI GTATCCnnnnn_n'
Cuts at: 4407 5168 6509 4407
Size: 761 1341 4407
Fragments arranged by size:
4407 1341 761
BclI T'GATC_A
Cuts at: 4029 4029
Size: 6509
BglI GCCn_nnn'nGGC
Cuts at: 182 711 182
Size: 529 5980
BglII A'GATC_T
Cuts at: 285 759 1824 3040 4024 6061 285
Size: 474 1065 1216 984 2037 733
Fragments arranged by size:
2037 1216 1065 984 733 474
BlpI GC'TnA_GC
Cuts at: 3621 3621
Size: 6509
Bme1580I G_kGCm'C
Cuts at: 1188 1974 4134 4227 5283 1188
Size: 786 2160 93 1056 2414
Fragments arranged by size:
2414 2160 1056 786 93
BmgBI CAC'GTC
Cuts at: 2019 2019
Size: 6509
BmrI ACTGGGnnnn_n'
Cuts at: 695 2817 3721 3802 4137 6281 695
Size: 2122 904 81 335 2144 923
Fragments arranged by size:
2144 2122 923 904 335 81
BmtI G_CTAG'C
Cuts at: 2734 2734
Size: 6509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4876 4876
Size: 6509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 764 3404 175
Size: 589 2640 3280
Fragments arranged by size:
3280 2640 589
Bpu10I CC'TnA_GC
Cuts at: 2796 2796
Size: 6509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 2371 3274 3685 4564 5056 5354 433
Size: 1938 903 411 879 492 298 1588
Fragments arranged by size:
1938 1588 903 879 492 411 298
BsaI GGTCTCn'nnnn_
Cuts at: 338 880 2402 3028 6213 338
Size: 542 1522 626 3185 634
Fragments arranged by size:
3185 1522 634 626 542
BsaAI yAC'GTr
Cuts at: 2058 3862 2058
Size: 1804 4705
BsaBI GATnn'nnATC
Cuts at: 6 4023 6060 6
Size: 4017 2037 455
Fragments arranged by size:
4017 2037 455
BsaHI Gr'CG_yC
Cuts at: 3940 4188 4890 6386 3940
Size: 248 702 1496 4063
Fragments arranged by size:
4063 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3698 4219 4897 5171 5318 5729 3698
Size: 521 678 274 147 411 4478
Fragments arranged by size:
4478 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3264 3294 3264
Size: 30 6479
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 600 1010 600
Size: 410 6099
BseYI C'CCAG_C
Cuts at: 1438 1657 1967 3934 4692 5269 5765 1438
Size: 219 310 1967 758 577 496 2182
Fragments arranged by size:
2182 1967 758 577 496 310 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 691 844 2173 189
Size: 502 153 1329 4525
Fragments arranged by size:
4525 1329 502 153
BsiEI CG_ry'CG
Cuts at: 104 702 4051 4097 5305 104
Size: 598 3349 46 1208 1308
Fragments arranged by size:
3349 1308 1208 598 46
BsiHKAI G_wGCw'C
Cuts at: 116 383 425 685 1346 3523 4301 4491 5283
Size: 267 42 260 661 2177 778 190 792
Cuts at: 5283 116
Size: 1342
Fragments arranged by size:
2177 1342 792 778 661 267 260 190
42
BsmI GAATG_Cn'
Cuts at: 983 1808 3201 983
Size: 825 1393 4291
Fragments arranged by size:
4291 1393 825
BsmBI CGTCTCn'nnnn_
Cuts at: 2009 2009
Size: 6509
Bsp1286I G_dGCh'C
Cuts at: 116 383 425 685 1188 1346 1974 3523 4134
Size: 267 42 260 503 158 628 1549 611
Cuts at: 4134 4227 4301 4491 4553 5283 116
Size: 93 74 190 62 730 1342
Fragments arranged by size:
1549 1342 730 628 611 503 267 260
190 158 93 74 62 42
BspEI T'CCGG_A
Cuts at: 5729 5729
Size: 6509
BspHI T'CATG_A
Cuts at: 5685 5685
Size: 6509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3559 3773 4075 4456 3559
Size: 214 302 381 5612
Fragments arranged by size:
5612 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4801 4855 6339 6504 50
Size: 4751 54 1484 165 55
Fragments arranged by size:
4751 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2227 2282 4421 2227
Size: 55 2139 4315
Fragments arranged by size:
4315 2139 55
BsrFI r'CCGG_y
Cuts at: 4507 4688 4897 4507
Size: 181 209 6119
Fragments arranged by size:
6119 209 181
BssHII G'CGCG_C
Cuts at: 156 158 156
Size: 2 6507
BssSI C'ACGA_G
Cuts at: 1085 4780 5138 1085
Size: 3695 358 2456
Fragments arranged by size:
3695 2456 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1293 3886 1293
Size: 2593 3916
BstBI TT'CG_AA
Cuts at: 1193 3330 4870 1193
Size: 2137 1540 2832
Fragments arranged by size:
2832 2137 1540
BstXI CCAn_nnnn'nTGG
Cuts at: 240 1029 2973 240
Size: 789 1944 3776
Fragments arranged by size:
3776 1944 789
BstYI r'GATC_y
Cuts at: 1 285 408 759 1824 3040 3534 4001 4024
Size: 284 123 351 1065 1216 494 467 23
Cuts at: 4024 4359 4605 5617 5703 5715 6061 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1216 1065 1012 494 467 449 351 346
335 284 246 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 404 896 404
Size: 492 6017
BtgI C'CryG_G
Cuts at: 107 292 1141 1959 2315 3338 3378 107
Size: 185 849 818 356 1023 40 3238
Fragments arranged by size:
3238 1023 849 818 356 185 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1286 1506 3125 4459 4640 4803 1286
Size: 220 1619 1334 181 163 2992
Fragments arranged by size:
2992 1619 1334 220 181 163
BtsI GCAGTG_nn'
Cuts at: 1344 3401 3752 1344
Size: 2057 351 4101
Fragments arranged by size:
4101 2057 351
ClaI AT'CG_AT
Cuts at: 1125 1125
Size: 6509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2139 2174 3164 3199 2139
Size: 35 990 35 5449
Fragments arranged by size:
5449 990 35 35
DraI TTT'AAA
Cuts at: 3508 3508
Size: 6509
DrdI GACnn_nn'nnGTC
Cuts at: 352 4215 5073 352
Size: 3863 858 1788
Fragments arranged by size:
3863 1788 858
EaeI y'GGCC_r
Cuts at: 3136 4094 4268 4659 4686 6031 3136
Size: 958 174 391 27 1345 3614
Fragments arranged by size:
3614 1345 958 391 174 27
EagI C'GGCC_G
Cuts at: 4094 4094
Size: 6509
EarI CTCTTCn'nnn_
Cuts at: 121 593 987 1248 2249 2353 3406 4532 4742
Size: 472 394 261 1001 104 1053 1126 210
Cuts at: 4742 121
Size: 1888
Fragments arranged by size:
1888 1126 1053 1001 472 394 261 210
104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4717 5027 5173 6319 4717
Size: 310 146 1146 4907
Fragments arranged by size:
4907 1146 310 146
EcoICRI GAG'CTC
Cuts at: 114 423 3521 114
Size: 309 3098 3102
Fragments arranged by size:
3102 3098 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 520 628 764 1303 1693 2260 2273 2648
Size: 345 108 136 539 390 567 13 375
Cuts at: 2648 2846 3269 3404 4334 4766 5513 5781 175
Size: 198 423 135 930 432 747 268 903
Fragments arranged by size:
930 903 747 567 539 432 423 390
375 345 268 198 136 135 108 13
EcoNI CCTnn'n_nnAGG
Cuts at: 145 145
Size: 6509
EcoO109I rG'GnC_Cy
Cuts at: 1971 2140 3648 1971
Size: 169 1508 4832
Fragments arranged by size:
4832 1508 169
EcoRI G'AATT_C
Cuts at: 2692 3513 2692
Size: 821 5688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1312 1698 2290 2593 4528 1312
Size: 386 592 303 1935 3293
Fragments arranged by size:
3293 1935 592 386 303
FspI TGC'GCA
Cuts at: 4290 4290
Size: 6509
HaeII r_GCGC'y
Cuts at: 2803 3943 4191 5213 6192 2803
Size: 1140 248 1022 979 3120
Fragments arranged by size:
3120 1140 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 613 645 684 716 744 762 776 794 879
Size: 32 39 32 28 18 14 18 85
Cuts at: 879 911 1653 1685 1739 1771 1809 1841 2659
Size: 32 742 32 54 32 38 32 818
Cuts at: 2659 2691 3025 3057 3117 3149 4351 4381 4383
Size: 32 334 32 60 32 1202 30 2
Cuts at: 4383 4413 613
Size: 30 2709
Fragments arranged by size:
2709 1202 818 742 334 85 60 54
39 38 32 32 32 32 32 32
32 32 32 30 30 28 18 18
14 2
HincII GTy'rAC
Cuts at: 2547 3548 2547
Size: 1001 5508
HindIII A'AGCT_T
Cuts at: 1862 1862
Size: 6509
HpaI GTT'AAC
Cuts at: 2547 2547
Size: 6509
KasI G'GCGC_C
Cuts at: 3939 4187 3939
Size: 248 6261
KpnI G_GTAC'C
Cuts at: 3529 3529
Size: 6509
MluI A'CGCG_T
Cuts at: 5942 5942
Size: 6509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 222 560 5180 5364 222
Size: 338 4620 184 1367
Fragments arranged by size:
4620 1367 338 184
MscI TGG'CCA
Cuts at: 4270 4270
Size: 6509
MslI CAynn'nnrTG
Cuts at: 1504 1813 2620 2687 2885 4498 4625 1504
Size: 309 807 67 198 1613 127 3388
Fragments arranged by size:
3388 1613 807 309 198 127 67
MspA1I CmG'CkG
Cuts at: 1143 1548 1666 3620 3934 4294 5307 5552 1143
Size: 405 118 1954 314 360 1013 245 2100
Fragments arranged by size:
2100 1954 1013 405 360 314 245 118
NaeI GCC'GGC
Cuts at: 4690 4690
Size: 6509
NarI GG'CG_CC
Cuts at: 3940 4188 3940
Size: 248 6261
NcoI C'CATG_G
Cuts at: 107 292 1959 2315 3338 3378 107
Size: 185 1667 356 1023 40 3238
Fragments arranged by size:
3238 1667 1023 356 185 40
NgoMIV G'CCGG_C
Cuts at: 4688 4688
Size: 6509
NheI G'CTAG_C
Cuts at: 2730 2730
Size: 6509
NruI TCG'CGA
Cuts at: 1525 1525
Size: 6509
NspI r_CATG'y
Cuts at: 2184 2661 3246 3562 4062 4969 2184
Size: 477 585 316 500 907 3724
Fragments arranged by size:
3724 907 585 500 477 316
PasI CC'CwG_GG
Cuts at: 2421 2421
Size: 6509
PciI A'CATG_T
Cuts at: 2657 4965 2657
Size: 2308 4201
PmeI GTTT'AAAC
Cuts at: 3508 3508
Size: 6509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5648 5680 5648
Size: 32 6477
PspOMI G'GGCC_C
Cuts at: 1970 1970
Size: 6509
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1568 1600 1568
Size: 32 6477
PstI C_TGCA'G
Cuts at: 146 2300 3556 4241 146
Size: 2154 1256 685 2414
Fragments arranged by size:
2414 2154 1256 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6509
PvuII CAG'CTG
Cuts at: 1548 1666 3934 4294 1548
Size: 118 2268 360 3763
Fragments arranged by size:
3763 2268 360 118
RsrII CG'GwC_CG
Cuts at: 4704 4704
Size: 6509
SacI G_AGCT'C
Cuts at: 116 425 3523 116
Size: 309 3098 3102
Fragments arranged by size:
3102 3098 309
SacII CC_GC'GG
Cuts at: 1144 1144
Size: 6509
SalI G'TCGA_C
Cuts at: 3546 3546
Size: 6509
SapI GCTCTTCn'nnn_
Cuts at: 121 2353 4532 4742 121
Size: 2232 2179 210 1888
Fragments arranged by size:
2232 2179 1888 210
SbfI CC_TGCA'GG
Cuts at: 3556 3556
Size: 6509
ScaI AGT'ACT
Cuts at: 6026 6026
Size: 6509
SfcI C'TryA_G
Cuts at: 33 142 2193 2296 3552 4237 5230 5421 33
Size: 109 2051 103 1256 685 993 191 1121
Fragments arranged by size:
2051 1256 1121 993 685 191 109 103
SfoI GGC'GCC
Cuts at: 3941 4189 3941
Size: 248 6261
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
SmaI CCC'GGG
Cuts at: 3531 3531
Size: 6509
SmlI C'TyrA_G
Cuts at: 412 2350 3253 3664 4579 5071 5333 412
Size: 1938 903 411 915 492 262 1588
Fragments arranged by size:
1938 1588 915 903 492 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6509
SphI G_CATG'C
Cuts at: 2184 3246 3562 4062 2184
Size: 1062 316 500 4631
Fragments arranged by size:
4631 1062 500 316
SspI AAT'ATT
Cuts at: 2496 2496
Size: 6509
StyI C'CwwG_G
Cuts at: 107 125 292 813 1844 1959 2315 2406 3338
Size: 18 167 521 1031 115 356 91 932
Cuts at: 3338 3378 3643 3997 107
Size: 40 265 354 2619
Fragments arranged by size:
2619 1031 932 521 356 354 265 167
115 91 40 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 743 941 2807 4092 4225 4684 4893 743
Size: 198 1866 1285 133 459 209 2359
Fragments arranged by size:
2359 1866 1285 459 209 198 133
TatI w'GTAC_w
Cuts at: 6024 6024
Size: 6509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1455 1487 6026 6058 1455
Size: 32 4539 32 1906
Fragments arranged by size:
4539 1906 32 32
Tth111I GACn'n_nGTC
Cuts at: 2108 4306 2108
Size: 2198 4311
XbaI T'CTAG_A
Cuts at: 2112 3540 2112
Size: 1428 5081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1527 2313 4004 1527
Size: 786 1691 4032
Fragments arranged by size:
4032 1691 786
XmaI C'CCGG_G
Cuts at: 3529 3529
Size: 6509
XmnI GAAnn'nnTTC
Cuts at: 613 613
Size: 6509
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PmeI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BcgI BfrBI BsiWI BsrGI BstEII BstZ17I DraIII
EcoRV FseI FspAI MfeI NdeI NotI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI