Restriction map for
pF1KA0950
(Circular) (Six-base) MAPSORT of: pF1KA0950.seq Check: 1616 from: 1 to: 4061
pF1KA0950 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 156 156
Size: 4061
AccI GT'mk_AC
Cuts at: 425 1099 425
Size: 674 3387
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 798 1886 2318 2671 3333 726
Size: 72 1088 432 353 662 1454
Fragments arranged by size:
1454 1088 662 432 353 72
AflIII A'CryG_T
Cuts at: 1413 3213 3494 1413
Size: 1800 281 1980
Fragments arranged by size:
1980 1800 281
AleI CACnn'nnGTG
Cuts at: 280 688 280
Size: 408 3653
AlwNI CAG_nnn'CTG
Cuts at: 450 933 1366 2804 450
Size: 483 433 1438 1707
Fragments arranged by size:
1707 1438 483 433
ApaLI G'TGCA_C
Cuts at: 2899 2899
Size: 4061
ApoI r'AATT_y
Cuts at: 1065 3414 3670 1065
Size: 2349 256 1456
Fragments arranged by size:
2349 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 282 1077 1491 1739 1774 282
Size: 795 414 248 35 2569
Fragments arranged by size:
2569 795 414 248 35
BanII G_rGCy'C
Cuts at: 368 964 987 1075 2105 368
Size: 596 23 88 1030 2324
Fragments arranged by size:
2324 1030 596 88 23
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 732 732
Size: 4061
BbvCI CC'TCA_GC
Cuts at: 204 204
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 3015 4061 1959
Size: 1056 1046 1959
Fragments arranged by size:
1959 1056 1046
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 958 1173 958
Size: 215 3846
Bme1580I G_kGCm'C
Cuts at: 203 860 1686 1779 2903 203
Size: 657 826 93 1124 1361
Fragments arranged by size:
1361 1124 826 657 93
BmrI ACTGGGnnnn_n'
Cuts at: 540 665 895 921 1273 1354 1689 3833 540
Size: 125 230 26 352 81 335 2144 768
Fragments arranged by size:
2144 768 352 335 230 125 81 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 312 176
Size: 136 3925
Bpu10I CC'TnA_GC
Cuts at: 204 204
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2830 3128 1237
Size: 879 714 298 2170
Fragments arranged by size:
2170 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 350 3765 350
Size: 3415 646
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 948 1492 1740 2442 3938 948
Size: 544 248 702 1496 1071
Fragments arranged by size:
1496 1071 702 544 248
BsaWI w'CCGG_w
Cuts at: 352 1250 1771 2449 2860 3007 3281 352
Size: 898 521 678 411 147 274 1132
Fragments arranged by size:
1132 898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 754 784 881 911 670
Size: 30 54 30 97 30 3820
Fragments arranged by size:
3820 97 54 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 982 982
Size: 4061
BseYI C'CCAG_C
Cuts at: 267 299 316 383 1486 2244 2909 3317 267
Size: 32 17 67 1103 758 665 408 1011
Fragments arranged by size:
1103 1011 758 665 408 67 32 17
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2879 104
Size: 1499 46 1230 1286
Fragments arranged by size:
1499 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 368 788 1075 1853 2043 2903 368
Size: 420 287 778 190 860 1526
Fragments arranged by size:
1526 860 778 420 287 190
Bsp1286I G_dGCh'C
Cuts at: 203 368 788 860 964 987 1075 1686 1779
Size: 165 420 72 104 23 88 611 93
Cuts at: 1779 1853 2043 2105 2903 203
Size: 74 190 62 798 1361
Fragments arranged by size:
1361 798 611 420 190 165 104 93
88 74 72 62 23
BspHI T'CATG_A
Cuts at: 800 2493 800
Size: 1693 2368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 1111 1325 1627 2008 156
Size: 955 214 302 381 2209
Fragments arranged by size:
2209 955 381 302 214
BsrBI CCG'CTC
Cuts at: 50 892 2353 2407 3891 4056 50
Size: 842 1461 54 1484 165 55
Fragments arranged by size:
1484 1461 842 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 2059 2240 2059
Size: 181 3880
BssSI C'ACGA_G
Cuts at: 2332 3040 2332
Size: 708 3353
BstAPI GCAn_nnn'nTGC
Cuts at: 168 541 877 1438 168
Size: 373 336 561 2791
Fragments arranged by size:
2791 561 373 336
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstEII G'GTnAC_C
Cuts at: 453 940 453
Size: 487 3574
BstYI r'GATC_y
Cuts at: 1 1086 1553 1576 1911 2157 2463 2475 2561
Size: 1085 467 23 335 246 306 12 86
Cuts at: 2561 3613 1
Size: 1052 449
Fragments arranged by size:
1085 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 965 965
Size: 4061
BtgI C'CryG_G
Cuts at: 128 641 128
Size: 513 3548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1304 203
Size: 1101 2960
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 3111 1767
Size: 1344 2717
EaeI y'GGCC_r
Cuts at: 830 1646 1820 2211 2238 3583 830
Size: 816 174 391 27 1345 1308
Fragments arranged by size:
1345 1308 816 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 373 793 2084 2294 373
Size: 420 1291 210 2140
Fragments arranged by size:
2140 1291 420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 3011 3157 3871 2269
Size: 742 146 714 2459
Fragments arranged by size:
2459 742 714 146
EcoICRI GAG'CTC
Cuts at: 366 1073 366
Size: 707 3354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 312 726 798 1886 2318 2671 3333 176
Size: 136 414 72 1088 432 353 662 904
Fragments arranged by size:
1088 904 662 432 414 353 136 72
EcoO109I rG'GnC_Cy
Cuts at: 141 257 1200 141
Size: 116 943 3002
Fragments arranged by size:
3002 943 116
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 780 2080 780
Size: 1300 2761
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2973 3744 1495
Size: 248 1230 771 1812
Fragments arranged by size:
1812 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 398 430 754 786 1903 1933 1935 1965 398
Size: 32 324 32 1117 30 2 30 2494
Fragments arranged by size:
2494 1117 324 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2820 3004 2820
Size: 184 3877
MscI TGG'CCA
Cuts at: 832 1822 832
Size: 990 3071
MslI CAynn'nnrTG
Cuts at: 280 658 688 2050 2177 280
Size: 378 30 1362 127 2164
Fragments arranged by size:
2164 1362 378 127 30
MspA1I CmG'CkG
Cuts at: 280 383 447 818 1172 1486 1846 2630 2875
Size: 103 64 371 354 314 360 784 245
Cuts at: 2875 280
Size: 1466
Fragments arranged by size:
1466 784 371 360 354 314 245 103
64
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 641 641
Size: 4061
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 3217 1114
Size: 500 1603 1958
Fragments arranged by size:
1958 1603 500
PasI CC'CwG_GG
Cuts at: 114 145 114
Size: 31 4030
PciI A'CATG_T
Cuts at: 3213 3213
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 2507
Size: 32 4029
PstI C_TGCA'G
Cuts at: 1034 1108 1793 1034
Size: 74 685 3302
Fragments arranged by size:
3302 685 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 383 447 1486 1846 383
Size: 64 1039 360 2598
Fragments arranged by size:
2598 1039 360 64
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 368 1075 368
Size: 707 3354
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 373 793 2084 2294 373
Size: 420 1291 210 2140
Fragments arranged by size:
2140 1291 420 210
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 1030 1104 1789 2757 2948 33
Size: 997 74 685 968 191 1146
Fragments arranged by size:
1146 997 968 685 191 74
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 1216 2131 2845 3107 1216
Size: 915 714 262 2170
Fragments arranged by size:
2170 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 458 641 1195 1549 458
Size: 183 554 354 2970
Fragments arranged by size:
2970 554 354 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 940 1644 1777 2236 2445 166
Size: 774 704 133 459 209 1782
Fragments arranged by size:
1782 774 704 459 209 133
TatI w'GTAC_w
Cuts at: 3576 3576
Size: 4061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 493 505 1858 493
Size: 12 1353 2696
Fragments arranged by size:
2696 1353 12
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 527 1556 527
Size: 1029 3032
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI