Restriction map for pF1KA0926
 (Circular) (Six-base) MAPSORT of: pF1KA0926.seq  Check: 9623  from: 1  to: 7400



pF1KA0926  7400 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1134    1134 
   Size:       7400
 
AccI GT'mk_AC

Cuts at:   1761    4438    1761 
   Size:       2677    4723
 
Acc65I G'GTAC_C

Cuts at:   4416    4416 
   Size:       7400
 
AclI AA'CG_TT

Cuts at:   2293    7240    2293 
   Size:       4947    2453
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     190    1191    2392    2557    2899    3042    4339    5225 
   Size:         15    1001    1201     165     342     143    1297     886

Cuts at:   5225    5657    6404    6672     175 
   Size:        432     747     268     903

  Fragments arranged by size: 

                1297    1201    1001     903     886     747     432     342
                 268     165     143      15
 
AflIII A'CryG_T

Cuts at:   2119    4752    5856    6833    2119 
   Size:       2633    1104     977    2686

  Fragments arranged by size: 

                2686    2633    1104     977
 
AgeI A'CCGG_T

Cuts at:   3264    5788    3264 
   Size:       2524    4876
 
AhdI GACnn_n'nnGTC

Cuts at:    606     606 
   Size:       7400
 
AleI CACnn'nnGTG

Cuts at:    451     649    3805     451 
   Size:        198    3156    4046

  Fragments arranged by size: 

                4046    3156     198
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1127    1127 
   Size:       7400
 
AlwNI CAG_nnn'CTG

Cuts at:    186    1302    2235    2706    2772    2802    2847    3018    3513 
   Size:       1116     933     471      66      30      45     171     495

Cuts at:   3513    4110    4197    4705    6272     186 
   Size:        597      87     508    1567    1314

  Fragments arranged by size: 

                1567    1314    1116     933     597     508     495     471
                 171      87      66      45      30
 
ApaI G_GGCC'C

Cuts at:   1653    3027    3352    1653 
   Size:       1374     325    5701

  Fragments arranged by size: 

                5701    1374     325
 
ApaLI G'TGCA_C

Cuts at:    489    2367    3598    6170     489 
   Size:       1878    1231    2572    1719

  Fragments arranged by size: 

                2572    1878    1719    1231
 
ApoI r'AATT_y

Cuts at:    746     937    2383    3490    4404    6753    7009     746 
   Size:        191    1446    1107     914    2349     256    1137

  Fragments arranged by size: 

                2349    1446    1137    1107     914     256     191
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7400
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7400
 
AvaI C'yCGr_G

Cuts at:   1456    4148    4420    1456 
   Size:       2692     272    4436

  Fragments arranged by size: 

                4436    2692     272
 
BamHI G'GATC_C

Cuts at:    467     667    4425     467 
   Size:        200    3758    3442

  Fragments arranged by size: 

                3758    3442     200
 
BanI G'GyrC_C

Cuts at:   4416    4830    5078    5113    4416 
   Size:        414     248      35    6703

  Fragments arranged by size: 

                6703     414     248      35
 
BanII G_rGCy'C

Cuts at:    217     502    1256    1385    1646    1653    2360    2449    2945 
   Size:        285     754     129     261       7     707      89     496

Cuts at:   2945    3027    3352    3902    4414    5444     217 
   Size:         82     325     550     512    1030    2173

  Fragments arranged by size: 

                2173    1030     754     707     550     512     496     325
                 285     261     129      89      82       7
 
BbeI G_GCGC'C

Cuts at:   4834    5082    4834 
   Size:        248    7152
 
BbsI GAAGACnn'nnnn_

Cuts at:    253    1191    3929     253 
   Size:        938    2738    3724

  Fragments arranged by size: 

                3724    2738     938
 
BbvCI CC'TCA_GC

Cuts at:    343     677    1835    2660    3044     343 
   Size:        334    1158     825     384    4699

  Fragments arranged by size: 

                4699    1158     825     384     334
 
BciVI GTATCCnnnnn_n'

Cuts at:   1068    3096    5298    6059    7400    1068 
   Size:       2028    2202     761    1341    1068

  Fragments arranged by size: 

                2202    2028    1341    1068     761
 
BclI T'GATC_A

Cuts at:   1472    4920    1472 
   Size:       3448    3952
 
BglI GCCn_nnn'nGGC

Cuts at:   1282    1317    1282 
   Size:         35    7365
 
BglII A'GATC_T

Cuts at:   3215    4035    4915    6952    3215 
   Size:        820     880    2037    3663

  Fragments arranged by size: 

                3663    2037     880     820
 
BlpI GC'TnA_GC

Cuts at:    236    1250    1790    2566    3575    4198    4512     236 
   Size:       1014     540     776    1009     623     314    3124

  Fragments arranged by size: 

                3124    1014    1009     776     623     540     314
 
Bme1580I G_kGCm'C

Cuts at:    493    1653    1667    2371    3027    3352    3602    5025    5118 
   Size:       1160      14     704     656     325     250    1423      93

Cuts at:   5118    6174     493 
   Size:       1056    1719

  Fragments arranged by size: 

                1719    1423    1160    1056     704     656     325     250
                  93      14
 
BmgBI CAC'GTC

Cuts at:   2828    3207    2828 
   Size:        379    7021
 
BmrI ACTGGGnnnn_n'

Cuts at:   1221    2473    4612    4693    5028    7172    1221 
   Size:       1252    2139      81     335    2144    1449

  Fragments arranged by size: 

                2144    2139    1449    1252     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    285     910    2627    3264    5767     285 
   Size:        625    1717     637    2503    1918

  Fragments arranged by size: 

                2503    1918    1717     637     625
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     570    1416    2275    2644    2768    3049    3602    3709 
   Size:        378     846     859     369     124     281     553     107

Cuts at:   3709    3916    3938    4049     192 
   Size:        207      22     111    3543

  Fragments arranged by size: 

                3543     859     846     553     378     369     281     207
                 124     111     107      22
 
Bpu10I CC'TnA_GC

Cuts at:    343     677    1835    2660    2722    2944    3044    3182     343 
   Size:        334    1158     825      62     222     100     138    4561

  Fragments arranged by size: 

                4561    1158     825     334     222     138     100      62
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2503    3121    3284    4290    4576    5455    5947    6245    2503 
   Size:        618     163    1006     286     879     492     298    3658

  Fragments arranged by size: 

                3658    1006     879     618     492     298     286     163
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    431    1808    2620    3454    4318    7104     431 
   Size:       1377     812     834     864    2786     727

  Fragments arranged by size: 

                2786    1377     864     834     812     727
 
BsaAI yAC'GTr

Cuts at:   2397    4753    2397 
   Size:       2356    5044
 
BsaBI GATnn'nnATC

Cuts at:      6    4914    6951       6 
   Size:       4908    2037     455

  Fragments arranged by size: 

                4908    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    543    2607    4831    5079    5781    7277     543 
   Size:       2064    2224     248     702    1496     666

  Fragments arranged by size: 

                2224    2064    1496     702     666     248
 
BsaWI w'CCGG_w

Cuts at:   3264    4286    4589    5110    5788    6062    6209    6620    3264 
   Size:       1022     303     521     678     274     147     411    4044

  Fragments arranged by size: 

                4044    1022     678     521     411     303     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1263    1269    1293    1299    1263 
   Size:          6      24       6    7364

  Fragments arranged by size: 

                7364      24       6       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    178     562    1036    3688     178 
   Size:        384     474    2652    3890

  Fragments arranged by size: 

                3890    2652     474     384
 
BseYI C'CCAG_C

Cuts at:    430     654     662     682    1425    1437    1805    2872    3736 
   Size:        224       8      20     743      12     368    1067     864

Cuts at:   3736    4243    4825    5583    6160    6656     430 
   Size:        507     582     758     577     496    1174

  Fragments arranged by size: 

                1174    1067     864     758     743     582     577     507
                 496     368     224      20      12       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1375    1671    4096    1375 
   Size:        296    2425    4679

  Fragments arranged by size: 

                4679    2425     296
 
BsiEI CG_ry'CG

Cuts at:    104    4942    4988    6196     104 
   Size:       4838      46    1208    1308

  Fragments arranged by size: 

                4838    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    217     493    1256    1385    1646    1946    2360    2371    2738 
   Size:        276     763     129     261     300     414      11     367

Cuts at:   2738    3602    3902    4414    5192    5382    6174     217 
   Size:        864     300     512     778     190     792    1443

  Fragments arranged by size: 

                1443     864     792     778     763     512     414     367
                 300     300     276     261     190     129      11
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2598    2598 
   Size:       7400
 
Bsp1286I G_dGCh'C

Cuts at:    217     493     502    1256    1385    1646    1653    1667    1946 
   Size:        276       9     754     129     261       7      14     279

Cuts at:   1946    2360    2371    2449    2738    2945    3027    3352    3602 
   Size:        414      11      78     289     207      82     325     250

Cuts at:   3602    3902    4414    5025    5118    5192    5382    5444    6174 
   Size:        300     512     611      93      74     190      62     730

Cuts at:   6174     217 
   Size:       1443

  Fragments arranged by size: 

                1443     754     730     611     512     414     325     300
                 289     279     276     261     250     207     190     129
                  93      82      78      74      62      14      11       9
                   7
 
BspEI T'CCGG_A

Cuts at:   6620    6620 
   Size:       7400
 
BspHI T'CATG_A

Cuts at:   6576    6576 
   Size:       7400
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1134    2900    3317    4450    4664    4966    5347    1134 
   Size:       1766     417    1133     214     302     381    3187

  Fragments arranged by size: 

                3187    1766    1133     417     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     235    1317    5692    5746    7230    7395      50 
   Size:        185    1082    4375      54    1484     165      55

  Fragments arranged by size: 

                4375    1484    1082     185     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2009    5312    2009 
   Size:       3303    4097
 
BsrFI r'CCGG_y

Cuts at:    480    1410    2183    3264    5398    5579    5788     480 
   Size:        930     773    1081    2134     181     209    2092

  Fragments arranged by size: 

                2134    2092    1081     930     773     209     181
 
BssSI C'ACGA_G

Cuts at:   4236    5671    6029    4236 
   Size:       1435     358    5607

  Fragments arranged by size: 

                5607    1435     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2235    2375    4110    4777    2235 
   Size:        140    1735     667    4858

  Fragments arranged by size: 

                4858    1735     667     140
 
BstBI TT'CG_AA

Cuts at:   2321    5761    2321 
   Size:       3440    3960
 
BstEII G'GTnAC_C

Cuts at:   2523    3474    2523 
   Size:        951    6449
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1177    1177 
   Size:       7400
 
BstYI r'GATC_y

Cuts at:      1     467     667     984    1298    3215    4035    4425    4892 
   Size:        466     200     317     314    1917     820     390     467

Cuts at:   4892    4915    5250    5496    6508    6594    6606    6952       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1917    1012     820     467     466     449     390     346
                 335     317     314     246     200      86      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:   1879    3836    1879 
   Size:       1957    5443
 
BtgI C'CryG_G

Cuts at:    107     805     868    2453    3353     107 
   Size:        698      63    1585     900    4154

  Fragments arranged by size: 

                4154    1585     900     698      63
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   5350    5531    5694    5350 
   Size:        181     163    7056

  Fragments arranged by size: 

                7056     181     163
 
BtsI GCAGTG_nn'

Cuts at:    458     656    1420    2178    2218    2265    2585    4643     458 
   Size:        198     764     758      40      47     320    2058    3215

  Fragments arranged by size: 

                3215    2058     764     758     320     198      47      40
            
 
DraI TTT'AAA

Cuts at:   3669    4399    3669 
   Size:        730    6670
 
DraIII CAC_nnn'GTG

Cuts at:   3615    4119    3615 
   Size:        504    6896
 
DrdI GACnn_nn'nnGTC

Cuts at:   5106    5964    5106 
   Size:        858    6542
 
EaeI y'GGCC_r

Cuts at:   1136    3951    4985    5159    5550    5577    6922    1136 
   Size:       2815    1034     174     391      27    1345    1614

  Fragments arranged by size: 

                2815    1614    1345    1034     391     174      27
 
EagI C'GGCC_G

Cuts at:   4985    4985 
   Size:       7400
 
EarI CTCTTCn'nnn_

Cuts at:    222     590    1330    2512    2578    5423    5633     222 
   Size:        368     740    1182      66    2845     210    1989

  Fragments arranged by size: 

                2845    1989    1182     740     368     210      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    131    1429    5608    5918    6064    7210     131 
   Size:       1298    4179     310     146    1146     321

  Fragments arranged by size: 

                4179    1298    1146     321     310     146
 
EcoICRI GAG'CTC

Cuts at:    215    1254    1383    1644    2358    3900    4412     215 
   Size:       1039     129     261     714    1542     512    3203

  Fragments arranged by size: 

                3203    1542    1039     714     512     261     129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     190     192     570    1191    1416    2275    2392    2557 
   Size:         15       2     378     621     225     859     117     165

Cuts at:   2557    2644    2768    2899    3042    3049    3602    3709    3916 
   Size:         87     124     131     143       7     553     107     207

Cuts at:   3916    3938    4049    4339    5225    5657    6404    6672     175 
   Size:         22     111     290     886     432     747     268     903

  Fragments arranged by size: 

                 903     886     859     747     621     553     432     378
                 290     268     225     207     165     143     131     124
                 117     111     107      87      22      15       7       2
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    157    1774    3031     157 
   Size:       1617    1257    4526

  Fragments arranged by size: 

                4526    1617    1257
 
EcoO109I rG'GnC_Cy

Cuts at:    309     716     800    1539    1650    1801    2217    2475    2673 
   Size:        407      84     739     111     151     416     258     198

Cuts at:   2673    2844    3023    3024    3096    3306    3348    3349    3358 
   Size:        171     179       1      72     210      42       1       9

Cuts at:   3358    3553    4539     309 
   Size:        195     986    3170

  Fragments arranged by size: 

                3170     986     739     416     407     258     210     198
                 195     179     171     151     111      84      72      42
                   9       1       1
 
EcoRI G'AATT_C

Cuts at:   3490    4404    3490 
   Size:        914    6486
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   5419    5419 
   Size:       7400
 
FspI TGC'GCA

Cuts at:   5181    5181 
   Size:       7400
 
HaeII r_GCGC'y

Cuts at:   4834    5082    6104    7083    4834 
   Size:        248    1022     979    5151

  Fragments arranged by size: 

                5151    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    219     251     592     624     724     756    1263    1295    1464 
   Size:         32     341      32     100      32     507      32     169

Cuts at:   1464    1476    1496    1508    1793    1807    1825    1839    2836 
   Size:         12      20      12     285      14      18      14     997

Cuts at:   2836    2868    3953    3985    4317    4349    5242    5272    5274 
   Size:         32    1085      32     332      32     893      30       2

Cuts at:   5274    5304     219 
   Size:         30    2315

  Fragments arranged by size: 

                2315    1085     997     893     507     341     332     285
                 169     100      32      32      32      32      32      32
                  32      30      30      20      18      14      14      12
                  12       2
 
HincII GTy'rAC

Cuts at:   1130    3381    4439    1130 
   Size:       2251    1058    4091

  Fragments arranged by size: 

                4091    2251    1058
 
HindIII A'AGCT_T

Cuts at:   2404    2404 
   Size:       7400
 
KasI G'GCGC_C

Cuts at:   4830    5078    4830 
   Size:        248    7152
 
KpnI G_GTAC'C

Cuts at:   4420    4420 
   Size:       7400
 
MfeI C'AATT_G

Cuts at:   3224    3224 
   Size:       7400
 
MluI A'CGCG_T

Cuts at:   6833    6833 
   Size:       7400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   6071    6255    6071 
   Size:        184    7216
 
MscI TGG'CCA

Cuts at:   1138    3953    5161    1138 
   Size:       2815    1208    3377

  Fragments arranged by size: 

                3377    2815    1208
 
MslI CAynn'nnrTG

Cuts at:    262     451     649    3805    5389    5516     262 
   Size:        189     198    3156    1584     127    2146

  Fragments arranged by size: 

                3156    2146    1584     198     189     127
 
MspA1I CmG'CkG

Cuts at:    300     525     548     814     948    1173    1211    2232    2412 
   Size:        225      23     266     134     225      38    1021     180

Cuts at:   2412    2659    2808    2816    3048    3434    3539    3930    4140 
   Size:        247     149       8     232     386     105     391     210

Cuts at:   4140    4511    4825    5185    6198    6443     300 
   Size:        371     314     360    1013     245    1257

  Fragments arranged by size: 

                1257    1021    1013     391     386     371     360     314
                 266     247     245     232     225     225     210     180
                 149     134     105      38      23       8
 
NaeI GCC'GGC

Cuts at:    482    1412    2185    5581     482 
   Size:        930     773    3396    2301

  Fragments arranged by size: 

                3396    2301     930     773
 
NarI GG'CG_CC

Cuts at:   4831    5079    4831 
   Size:        248    7152
 
NcoI C'CATG_G

Cuts at:    107     805     868    2453     107 
   Size:        698      63    1585    5054

  Fragments arranged by size: 

                5054    1585     698      63
 
NdeI CA'TA_TG

Cuts at:   2085    2085 
   Size:       7400
 
NgoMIV G'CCGG_C

Cuts at:    480    1410    2183    5579     480 
   Size:        930     773    3396    2301

  Fragments arranged by size: 

                3396    2301     930     773
 
NspI r_CATG'y

Cuts at:   1198    2337    4453    4953    5860    1198 
   Size:       1139    2116     500     907    2738

  Fragments arranged by size: 

                2738    2116    1139     907     500
 
PasI CC'CwG_GG

Cuts at:    495    1667     495 
   Size:       1172    6228
 
PciI A'CATG_T

Cuts at:   5856    5856 
   Size:       7400
 
PflMI CCAn_nnn'nTGG

Cuts at:   2847    3513    2847 
   Size:        666    6734
 
PfoI T'CCnGG_A

Cuts at:    208    2621    3200    3686     208 
   Size:       2413     579     486    3922

  Fragments arranged by size: 

                3922    2413     579     486
 
PmeI GTTT'AAAC

Cuts at:   4399    4399 
   Size:       7400
 
PmlI CAC'GTG

Cuts at:   2397    2397 
   Size:       7400
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    882     914    3884    3916    6539    6571     882 
   Size:         32    2970      32    2623      32    1711

  Fragments arranged by size: 

                2970    2623    1711      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    309     716    1539    1801    2217    2475    2673    2844    3306 
   Size:        407     823     262     416     258     198     171     462

Cuts at:   3306     309 
   Size:       4403

  Fragments arranged by size: 

                4403     823     462     416     407     262     258     198
                 171
 
PspOMI G'GGCC_C

Cuts at:   1649    3023    3348    1649 
   Size:       1374     325    5701

  Fragments arranged by size: 

                5701    1374     325
 
PstI C_TGCA'G

Cuts at:   1106    1216    1499    2231    2243    2580    2807    2897    4447 
   Size:        110     283     732      12     337     227      90    1550

Cuts at:   4447    5132    1106 
   Size:        685    3374

  Fragments arranged by size: 

                3374    1550     732     685     337     283     227     110
                  90      12
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7400
 
PvuII CAG'CTG

Cuts at:    525     948    1173    1211    2232    2412    2659    2808    2816 
   Size:        423     225      38    1021     180     247     149       8

Cuts at:   2816    3048    3539    3930    4140    4825    5185     525 
   Size:        232     491     391     210     685     360    2740

  Fragments arranged by size: 

                2740    1021     685     491     423     391     360     247
                 232     225     210     180     149      38       8
 
RsrII CG'GwC_CG

Cuts at:   5595    5595 
   Size:       7400
 
SacI G_AGCT'C

Cuts at:    217    1256    1385    1646    2360    3902    4414     217 
   Size:       1039     129     261     714    1542     512    3203

  Fragments arranged by size: 

                3203    1542    1039     714     512     261     129
 
SalI G'TCGA_C

Cuts at:   4437    4437 
   Size:       7400
 
SanDI GG'GwC_CC

Cuts at:    716    1801    2217    2475     716 
   Size:       1085     416     258    5641

  Fragments arranged by size: 

                5641    1085     416     258
 
SapI GCTCTTCn'nnn_

Cuts at:    222     590    1330    5423    5633     222 
   Size:        368     740    4093     210    1989

  Fragments arranged by size: 

                4093    1989     740     368     210
 
SbfI CC_TGCA'GG

Cuts at:   4447    4447 
   Size:       7400
 
ScaI AGT'ACT

Cuts at:   6917    6917 
   Size:       7400
 
SexAI A'CCwGG_T

Cuts at:    275     275 
   Size:       7400
 
SfcI C'TryA_G

Cuts at:     33     399     850    1102    1212    1495    1578    1962    2227 
   Size:        366     451     252     110     283      83     384     265

Cuts at:   2227    2239    2576    2794    2803    2893    3416    4443    5128 
   Size:         12     337     218       9      90     523    1027     685

Cuts at:   5128    6121    6312      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121    1027     993     685     523     451     384     366
                 337     283     265     252     218     191     110      90
                  83      12       9
 
SfoI GGC'GCC

Cuts at:   4832    5080    4832 
   Size:        248    7152
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7400
 
SmaI CCC'GGG

Cuts at:   4422    4422 
   Size:       7400
 
SmlI C'TyrA_G

Cuts at:   2518    3136    3299    4269    4555    5470    5962    6224    2518 
   Size:        618     163     970     286     915     492     262    3694

  Fragments arranged by size: 

                3694     970     915     618     492     286     262     163
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7400
 
SphI G_CATG'C

Cuts at:   4453    4953    4453 
   Size:        500    6900
 
SspI AAT'ATT

Cuts at:   1573    1573 
   Size:       7400
 
StuI AGG'CCT

Cuts at:   1070    4018    1070 
   Size:       2948    4452
 
StyI C'CwwG_G

Cuts at:    107     805     868    1232    2453    3624    3749    4019    4534 
   Size:        698      63     364    1221    1171     125     270     515

Cuts at:   4534    4888     107 
   Size:        354    2619

  Fragments arranged by size: 

                2619    1221    1171     698     515     364     354     270
                 125      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    508     642     708     881    1659    2725    3505    4983    5116 
   Size:        134      66     173     778    1066     780    1478     133

Cuts at:   5116    5575    5784     508 
   Size:        459     209    2124

  Fragments arranged by size: 

                2124    1478    1066     780     778     459     209     173
                 134     133      66
 
TatI w'GTAC_w

Cuts at:   6915    6915 
   Size:       7400
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1269    1301    3060    3092    3262    3294    4276    4308    6917 
   Size:         32    1759      32     170      32     982      32    2609

Cuts at:   6917    6949    1269 
   Size:         32    1720

  Fragments arranged by size: 

                2609    1759    1720     982     170      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   5197    5197 
   Size:       7400
 
XbaI T'CTAG_A

Cuts at:   4431    4431 
   Size:       7400
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    651    3360    3536    4895     651 
   Size:       2709     176    1359    3156

  Fragments arranged by size: 

                3156    2709    1359     176
 
XmaI C'CCGG_G

Cuts at:   4420    4420 
   Size:       7400
 
XmnI GAAnn'nnTTC

Cuts at:    175    4261     175 
   Size:       4086    3314
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AloI     AscI    AvrII     BaeI     BcgI
    BfrBI     BmtI    BsiWI     BsmI    BsrGI   BssHII  BstZ17I     ClaI
    CspCI    EcoRV     FseI    FspAI     HpaI     NheI     NotI     NruI
     NsiI     PacI    PshAI     PsiI    PspXI     PsrI    SacII     SfiI
    SgrAI    SnaBI     SrfI     SwaI     XhoI     ZraI
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