(Circular) (Six-base) MAPSORT of: pF1KA0926.seq Check: 9623 from: 1 to: 7400 pF1KA0926 7400 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1134 1134 Size: 7400 AccI GT'mk_AC Cuts at: 1761 4438 1761 Size: 2677 4723 Acc65I G'GTAC_C Cuts at: 4416 4416 Size: 7400 AclI AA'CG_TT Cuts at: 2293 7240 2293 Size: 4947 2453 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 175 190 1191 2392 2557 2899 3042 4339 5225 Size: 15 1001 1201 165 342 143 1297 886 Cuts at: 5225 5657 6404 6672 175 Size: 432 747 268 903 Fragments arranged by size: 1297 1201 1001 903 886 747 432 342 268 165 143 15 AflIII A'CryG_T Cuts at: 2119 4752 5856 6833 2119 Size: 2633 1104 977 2686 Fragments arranged by size: 2686 2633 1104 977 AgeI A'CCGG_T Cuts at: 3264 5788 3264 Size: 2524 4876 AhdI GACnn_n'nnGTC Cuts at: 606 606 Size: 7400 AleI CACnn'nnGTG Cuts at: 451 649 3805 451 Size: 198 3156 4046 Fragments arranged by size: 4046 3156 198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1127 1127 Size: 7400 AlwNI CAG_nnn'CTG Cuts at: 186 1302 2235 2706 2772 2802 2847 3018 3513 Size: 1116 933 471 66 30 45 171 495 Cuts at: 3513 4110 4197 4705 6272 186 Size: 597 87 508 1567 1314 Fragments arranged by size: 1567 1314 1116 933 597 508 495 471 171 87 66 45 30 ApaI G_GGCC'C Cuts at: 1653 3027 3352 1653 Size: 1374 325 5701 Fragments arranged by size: 5701 1374 325 ApaLI G'TGCA_C Cuts at: 489 2367 3598 6170 489 Size: 1878 1231 2572 1719 Fragments arranged by size: 2572 1878 1719 1231 ApoI r'AATT_y Cuts at: 746 937 2383 3490 4404 6753 7009 746 Size: 191 1446 1107 914 2349 256 1137 Fragments arranged by size: 2349 1446 1137 1107 914 256 191 AseI AT'TA_AT Cuts at: 20 20 Size: 7400 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7400 AvaI C'yCGr_G Cuts at: 1456 4148 4420 1456 Size: 2692 272 4436 Fragments arranged by size: 4436 2692 272 BamHI G'GATC_C Cuts at: 467 667 4425 467 Size: 200 3758 3442 Fragments arranged by size: 3758 3442 200 BanI G'GyrC_C Cuts at: 4416 4830 5078 5113 4416 Size: 414 248 35 6703 Fragments arranged by size: 6703 414 248 35 BanII G_rGCy'C Cuts at: 217 502 1256 1385 1646 1653 2360 2449 2945 Size: 285 754 129 261 7 707 89 496 Cuts at: 2945 3027 3352 3902 4414 5444 217 Size: 82 325 550 512 1030 2173 Fragments arranged by size: 2173 1030 754 707 550 512 496 325 285 261 129 89 82 7 BbeI G_GCGC'C Cuts at: 4834 5082 4834 Size: 248 7152 BbsI GAAGACnn'nnnn_ Cuts at: 253 1191 3929 253 Size: 938 2738 3724 Fragments arranged by size: 3724 2738 938 BbvCI CC'TCA_GC Cuts at: 343 677 1835 2660 3044 343 Size: 334 1158 825 384 4699 Fragments arranged by size: 4699 1158 825 384 334 BciVI GTATCCnnnnn_n' Cuts at: 1068 3096 5298 6059 7400 1068 Size: 2028 2202 761 1341 1068 Fragments arranged by size: 2202 2028 1341 1068 761 BclI T'GATC_A Cuts at: 1472 4920 1472 Size: 3448 3952 BglI GCCn_nnn'nGGC Cuts at: 1282 1317 1282 Size: 35 7365 BglII A'GATC_T Cuts at: 3215 4035 4915 6952 3215 Size: 820 880 2037 3663 Fragments arranged by size: 3663 2037 880 820 BlpI GC'TnA_GC Cuts at: 236 1250 1790 2566 3575 4198 4512 236 Size: 1014 540 776 1009 623 314 3124 Fragments arranged by size: 3124 1014 1009 776 623 540 314 Bme1580I G_kGCm'C Cuts at: 493 1653 1667 2371 3027 3352 3602 5025 5118 Size: 1160 14 704 656 325 250 1423 93 Cuts at: 5118 6174 493 Size: 1056 1719 Fragments arranged by size: 1719 1423 1160 1056 704 656 325 250 93 14 BmgBI CAC'GTC Cuts at: 2828 3207 2828 Size: 379 7021 BmrI ACTGGGnnnn_n' Cuts at: 1221 2473 4612 4693 5028 7172 1221 Size: 1252 2139 81 335 2144 1449 Fragments arranged by size: 2144 2139 1449 1252 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 285 910 2627 3264 5767 285 Size: 625 1717 637 2503 1918 Fragments arranged by size: 2503 1918 1717 637 625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 192 570 1416 2275 2644 2768 3049 3602 3709 Size: 378 846 859 369 124 281 553 107 Cuts at: 3709 3916 3938 4049 192 Size: 207 22 111 3543 Fragments arranged by size: 3543 859 846 553 378 369 281 207 124 111 107 22 Bpu10I CC'TnA_GC Cuts at: 343 677 1835 2660 2722 2944 3044 3182 343 Size: 334 1158 825 62 222 100 138 4561 Fragments arranged by size: 4561 1158 825 334 222 138 100 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2503 3121 3284 4290 4576 5455 5947 6245 2503 Size: 618 163 1006 286 879 492 298 3658 Fragments arranged by size: 3658 1006 879 618 492 298 286 163 BsaI GGTCTCn'nnnn_ Cuts at: 431 1808 2620 3454 4318 7104 431 Size: 1377 812 834 864 2786 727 Fragments arranged by size: 2786 1377 864 834 812 727 BsaAI yAC'GTr Cuts at: 2397 4753 2397 Size: 2356 5044 BsaBI GATnn'nnATC Cuts at: 6 4914 6951 6 Size: 4908 2037 455 Fragments arranged by size: 4908 2037 455 BsaHI Gr'CG_yC Cuts at: 543 2607 4831 5079 5781 7277 543 Size: 2064 2224 248 702 1496 666 Fragments arranged by size: 2224 2064 1496 702 666 248 BsaWI w'CCGG_w Cuts at: 3264 4286 4589 5110 5788 6062 6209 6620 3264 Size: 1022 303 521 678 274 147 411 4044 Fragments arranged by size: 4044 1022 678 521 411 303 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1263 1269 1293 1299 1263 Size: 6 24 6 7364 Fragments arranged by size: 7364 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 178 562 1036 3688 178 Size: 384 474 2652 3890 Fragments arranged by size: 3890 2652 474 384 BseYI C'CCAG_C Cuts at: 430 654 662 682 1425 1437 1805 2872 3736 Size: 224 8 20 743 12 368 1067 864 Cuts at: 3736 4243 4825 5583 6160 6656 430 Size: 507 582 758 577 496 1174 Fragments arranged by size: 1174 1067 864 758 743 582 577 507 496 368 224 20 12 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1375 1671 4096 1375 Size: 296 2425 4679 Fragments arranged by size: 4679 2425 296 BsiEI CG_ry'CG Cuts at: 104 4942 4988 6196 104 Size: 4838 46 1208 1308 Fragments arranged by size: 4838 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 217 493 1256 1385 1646 1946 2360 2371 2738 Size: 276 763 129 261 300 414 11 367 Cuts at: 2738 3602 3902 4414 5192 5382 6174 217 Size: 864 300 512 778 190 792 1443 Fragments arranged by size: 1443 864 792 778 763 512 414 367 300 300 276 261 190 129 11 BsmBI CGTCTCn'nnnn_ Cuts at: 2598 2598 Size: 7400 Bsp1286I G_dGCh'C Cuts at: 217 493 502 1256 1385 1646 1653 1667 1946 Size: 276 9 754 129 261 7 14 279 Cuts at: 1946 2360 2371 2449 2738 2945 3027 3352 3602 Size: 414 11 78 289 207 82 325 250 Cuts at: 3602 3902 4414 5025 5118 5192 5382 5444 6174 Size: 300 512 611 93 74 190 62 730 Cuts at: 6174 217 Size: 1443 Fragments arranged by size: 1443 754 730 611 512 414 325 300 289 279 276 261 250 207 190 129 93 82 78 74 62 14 11 9 7 BspEI T'CCGG_A Cuts at: 6620 6620 Size: 7400 BspHI T'CATG_A Cuts at: 6576 6576 Size: 7400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1134 2900 3317 4450 4664 4966 5347 1134 Size: 1766 417 1133 214 302 381 3187 Fragments arranged by size: 3187 1766 1133 417 381 302 214 BsrBI CCG'CTC Cuts at: 50 235 1317 5692 5746 7230 7395 50 Size: 185 1082 4375 54 1484 165 55 Fragments arranged by size: 4375 1484 1082 185 165 55 54 BsrDI GCAATG_nn' Cuts at: 2009 5312 2009 Size: 3303 4097 BsrFI r'CCGG_y Cuts at: 480 1410 2183 3264 5398 5579 5788 480 Size: 930 773 1081 2134 181 209 2092 Fragments arranged by size: 2134 2092 1081 930 773 209 181 BssSI C'ACGA_G Cuts at: 4236 5671 6029 4236 Size: 1435 358 5607 Fragments arranged by size: 5607 1435 358 BstAPI GCAn_nnn'nTGC Cuts at: 2235 2375 4110 4777 2235 Size: 140 1735 667 4858 Fragments arranged by size: 4858 1735 667 140 BstBI TT'CG_AA Cuts at: 2321 5761 2321 Size: 3440 3960 BstEII G'GTnAC_C Cuts at: 2523 3474 2523 Size: 951 6449 BstXI CCAn_nnnn'nTGG Cuts at: 1177 1177 Size: 7400 BstYI r'GATC_y Cuts at: 1 467 667 984 1298 3215 4035 4425 4892 Size: 466 200 317 314 1917 820 390 467 Cuts at: 4892 4915 5250 5496 6508 6594 6606 6952 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1917 1012 820 467 466 449 390 346 335 317 314 246 200 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1879 3836 1879 Size: 1957 5443 BtgI C'CryG_G Cuts at: 107 805 868 2453 3353 107 Size: 698 63 1585 900 4154 Fragments arranged by size: 4154 1585 900 698 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5350 5531 5694 5350 Size: 181 163 7056 Fragments arranged by size: 7056 181 163 BtsI GCAGTG_nn' Cuts at: 458 656 1420 2178 2218 2265 2585 4643 458 Size: 198 764 758 40 47 320 2058 3215 Fragments arranged by size: 3215 2058 764 758 320 198 47 40 DraI TTT'AAA Cuts at: 3669 4399 3669 Size: 730 6670 DraIII CAC_nnn'GTG Cuts at: 3615 4119 3615 Size: 504 6896 DrdI GACnn_nn'nnGTC Cuts at: 5106 5964 5106 Size: 858 6542 EaeI y'GGCC_r Cuts at: 1136 3951 4985 5159 5550 5577 6922 1136 Size: 2815 1034 174 391 27 1345 1614 Fragments arranged by size: 2815 1614 1345 1034 391 174 27 EagI C'GGCC_G Cuts at: 4985 4985 Size: 7400 EarI CTCTTCn'nnn_ Cuts at: 222 590 1330 2512 2578 5423 5633 222 Size: 368 740 1182 66 2845 210 1989 Fragments arranged by size: 2845 1989 1182 740 368 210 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 131 1429 5608 5918 6064 7210 131 Size: 1298 4179 310 146 1146 321 Fragments arranged by size: 4179 1298 1146 321 310 146 EcoICRI GAG'CTC Cuts at: 215 1254 1383 1644 2358 3900 4412 215 Size: 1039 129 261 714 1542 512 3203 Fragments arranged by size: 3203 1542 1039 714 512 261 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 175 190 192 570 1191 1416 2275 2392 2557 Size: 15 2 378 621 225 859 117 165 Cuts at: 2557 2644 2768 2899 3042 3049 3602 3709 3916 Size: 87 124 131 143 7 553 107 207 Cuts at: 3916 3938 4049 4339 5225 5657 6404 6672 175 Size: 22 111 290 886 432 747 268 903 Fragments arranged by size: 903 886 859 747 621 553 432 378 290 268 225 207 165 143 131 124 117 111 107 87 22 15 7 2 EcoNI CCTnn'n_nnAGG Cuts at: 157 1774 3031 157 Size: 1617 1257 4526 Fragments arranged by size: 4526 1617 1257 EcoO109I rG'GnC_Cy Cuts at: 309 716 800 1539 1650 1801 2217 2475 2673 Size: 407 84 739 111 151 416 258 198 Cuts at: 2673 2844 3023 3024 3096 3306 3348 3349 3358 Size: 171 179 1 72 210 42 1 9 Cuts at: 3358 3553 4539 309 Size: 195 986 3170 Fragments arranged by size: 3170 986 739 416 407 258 210 198 195 179 171 151 111 84 72 42 9 1 1 EcoRI G'AATT_C Cuts at: 3490 4404 3490 Size: 914 6486 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5419 5419 Size: 7400 FspI TGC'GCA Cuts at: 5181 5181 Size: 7400 HaeII r_GCGC'y Cuts at: 4834 5082 6104 7083 4834 Size: 248 1022 979 5151 Fragments arranged by size: 5151 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 219 251 592 624 724 756 1263 1295 1464 Size: 32 341 32 100 32 507 32 169 Cuts at: 1464 1476 1496 1508 1793 1807 1825 1839 2836 Size: 12 20 12 285 14 18 14 997 Cuts at: 2836 2868 3953 3985 4317 4349 5242 5272 5274 Size: 32 1085 32 332 32 893 30 2 Cuts at: 5274 5304 219 Size: 30 2315 Fragments arranged by size: 2315 1085 997 893 507 341 332 285 169 100 32 32 32 32 32 32 32 30 30 20 18 14 14 12 12 2 HincII GTy'rAC Cuts at: 1130 3381 4439 1130 Size: 2251 1058 4091 Fragments arranged by size: 4091 2251 1058 HindIII A'AGCT_T Cuts at: 2404 2404 Size: 7400 KasI G'GCGC_C Cuts at: 4830 5078 4830 Size: 248 7152 KpnI G_GTAC'C Cuts at: 4420 4420 Size: 7400 MfeI C'AATT_G Cuts at: 3224 3224 Size: 7400 MluI A'CGCG_T Cuts at: 6833 6833 Size: 7400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 6071 6255 6071 Size: 184 7216 MscI TGG'CCA Cuts at: 1138 3953 5161 1138 Size: 2815 1208 3377 Fragments arranged by size: 3377 2815 1208 MslI CAynn'nnrTG Cuts at: 262 451 649 3805 5389 5516 262 Size: 189 198 3156 1584 127 2146 Fragments arranged by size: 3156 2146 1584 198 189 127 MspA1I CmG'CkG Cuts at: 300 525 548 814 948 1173 1211 2232 2412 Size: 225 23 266 134 225 38 1021 180 Cuts at: 2412 2659 2808 2816 3048 3434 3539 3930 4140 Size: 247 149 8 232 386 105 391 210 Cuts at: 4140 4511 4825 5185 6198 6443 300 Size: 371 314 360 1013 245 1257 Fragments arranged by size: 1257 1021 1013 391 386 371 360 314 266 247 245 232 225 225 210 180 149 134 105 38 23 8 NaeI GCC'GGC Cuts at: 482 1412 2185 5581 482 Size: 930 773 3396 2301 Fragments arranged by size: 3396 2301 930 773 NarI GG'CG_CC Cuts at: 4831 5079 4831 Size: 248 7152 NcoI C'CATG_G Cuts at: 107 805 868 2453 107 Size: 698 63 1585 5054 Fragments arranged by size: 5054 1585 698 63 NdeI CA'TA_TG Cuts at: 2085 2085 Size: 7400 NgoMIV G'CCGG_C Cuts at: 480 1410 2183 5579 480 Size: 930 773 3396 2301 Fragments arranged by size: 3396 2301 930 773 NspI r_CATG'y Cuts at: 1198 2337 4453 4953 5860 1198 Size: 1139 2116 500 907 2738 Fragments arranged by size: 2738 2116 1139 907 500 PasI CC'CwG_GG Cuts at: 495 1667 495 Size: 1172 6228 PciI A'CATG_T Cuts at: 5856 5856 Size: 7400 PflMI CCAn_nnn'nTGG Cuts at: 2847 3513 2847 Size: 666 6734 PfoI T'CCnGG_A Cuts at: 208 2621 3200 3686 208 Size: 2413 579 486 3922 Fragments arranged by size: 3922 2413 579 486 PmeI GTTT'AAAC Cuts at: 4399 4399 Size: 7400 PmlI CAC'GTG Cuts at: 2397 2397 Size: 7400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 882 914 3884 3916 6539 6571 882 Size: 32 2970 32 2623 32 1711 Fragments arranged by size: 2970 2623 1711 32 32 32 PpuMI rG'GwC_Cy Cuts at: 309 716 1539 1801 2217 2475 2673 2844 3306 Size: 407 823 262 416 258 198 171 462 Cuts at: 3306 309 Size: 4403 Fragments arranged by size: 4403 823 462 416 407 262 258 198 171 PspOMI G'GGCC_C Cuts at: 1649 3023 3348 1649 Size: 1374 325 5701 Fragments arranged by size: 5701 1374 325 PstI C_TGCA'G Cuts at: 1106 1216 1499 2231 2243 2580 2807 2897 4447 Size: 110 283 732 12 337 227 90 1550 Cuts at: 4447 5132 1106 Size: 685 3374 Fragments arranged by size: 3374 1550 732 685 337 283 227 110 90 12 PvuI CG_AT'CG Cuts at: 104 104 Size: 7400 PvuII CAG'CTG Cuts at: 525 948 1173 1211 2232 2412 2659 2808 2816 Size: 423 225 38 1021 180 247 149 8 Cuts at: 2816 3048 3539 3930 4140 4825 5185 525 Size: 232 491 391 210 685 360 2740 Fragments arranged by size: 2740 1021 685 491 423 391 360 247 232 225 210 180 149 38 8 RsrII CG'GwC_CG Cuts at: 5595 5595 Size: 7400 SacI G_AGCT'C Cuts at: 217 1256 1385 1646 2360 3902 4414 217 Size: 1039 129 261 714 1542 512 3203 Fragments arranged by size: 3203 1542 1039 714 512 261 129 SalI G'TCGA_C Cuts at: 4437 4437 Size: 7400 SanDI GG'GwC_CC Cuts at: 716 1801 2217 2475 716 Size: 1085 416 258 5641 Fragments arranged by size: 5641 1085 416 258 SapI GCTCTTCn'nnn_ Cuts at: 222 590 1330 5423 5633 222 Size: 368 740 4093 210 1989 Fragments arranged by size: 4093 1989 740 368 210 SbfI CC_TGCA'GG Cuts at: 4447 4447 Size: 7400 ScaI AGT'ACT Cuts at: 6917 6917 Size: 7400 SexAI A'CCwGG_T Cuts at: 275 275 Size: 7400 SfcI C'TryA_G Cuts at: 33 399 850 1102 1212 1495 1578 1962 2227 Size: 366 451 252 110 283 83 384 265 Cuts at: 2227 2239 2576 2794 2803 2893 3416 4443 5128 Size: 12 337 218 9 90 523 1027 685 Cuts at: 5128 6121 6312 33 Size: 993 191 1121 Fragments arranged by size: 1121 1027 993 685 523 451 384 366 337 283 265 252 218 191 110 90 83 12 9 SfoI GGC'GCC Cuts at: 4832 5080 4832 Size: 248 7152 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7400 SmaI CCC'GGG Cuts at: 4422 4422 Size: 7400 SmlI C'TyrA_G Cuts at: 2518 3136 3299 4269 4555 5470 5962 6224 2518 Size: 618 163 970 286 915 492 262 3694 Fragments arranged by size: 3694 970 915 618 492 286 262 163 SpeI A'CTAG_T Cuts at: 67 67 Size: 7400 SphI G_CATG'C Cuts at: 4453 4953 4453 Size: 500 6900 SspI AAT'ATT Cuts at: 1573 1573 Size: 7400 StuI AGG'CCT Cuts at: 1070 4018 1070 Size: 2948 4452 StyI C'CwwG_G Cuts at: 107 805 868 1232 2453 3624 3749 4019 4534 Size: 698 63 364 1221 1171 125 270 515 Cuts at: 4534 4888 107 Size: 354 2619 Fragments arranged by size: 2619 1221 1171 698 515 364 354 270 125 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 508 642 708 881 1659 2725 3505 4983 5116 Size: 134 66 173 778 1066 780 1478 133 Cuts at: 5116 5575 5784 508 Size: 459 209 2124 Fragments arranged by size: 2124 1478 1066 780 778 459 209 173 134 133 66 TatI w'GTAC_w Cuts at: 6915 6915 Size: 7400 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1269 1301 3060 3092 3262 3294 4276 4308 6917 Size: 32 1759 32 170 32 982 32 2609 Cuts at: 6917 6949 1269 Size: 32 1720 Fragments arranged by size: 2609 1759 1720 982 170 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5197 5197 Size: 7400 XbaI T'CTAG_A Cuts at: 4431 4431 Size: 7400 XcmI CCAnnnn_n'nnnnTGG Cuts at: 651 3360 3536 4895 651 Size: 2709 176 1359 3156 Fragments arranged by size: 3156 2709 1359 176 XmaI C'CCGG_G Cuts at: 4420 4420 Size: 7400 XmnI GAAnn'nnTTC Cuts at: 175 4261 175 Size: 4086 3314 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AloI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI NruI NsiI PacI PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI