Restriction map for
pF1KA0919
(Circular) (Six-base) MAPSORT of: pF1KA0919.seq Check: 5935 from: 1 to: 5129
pF1KA0919 5129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 468 1002 2036 2167 468
Size: 534 1034 131 3430
Fragments arranged by size:
3430 1034 534 131
Acc65I G'GTAC_C
Cuts at: 954 2145 954
Size: 1191 3938
AclI AA'CG_TT
Cuts at: 4969 4969
Size: 5129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 1327 1654 1688 1946 2954 3386 3739 4401
Size: 849 327 34 258 1008 432 353 662
Cuts at: 4401 478
Size: 1206
Fragments arranged by size:
1206 1008 849 662 432 353 327 258
34
AflIII A'CryG_T
Cuts at: 2481 4281 4562 2481
Size: 1800 281 3048
Fragments arranged by size:
3048 1800 281
AleI CACnn'nnGTG
Cuts at: 1852 1852
Size: 5129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 596 1747 596
Size: 1151 3978
AlwNI CAG_nnn'CTG
Cuts at: 2434 3872 2434
Size: 1438 3691
ApaI G_GGCC'C
Cuts at: 212 966 212
Size: 754 4375
ApaLI G'TGCA_C
Cuts at: 3967 3967
Size: 5129
ApoI r'AATT_y
Cuts at: 917 1582 1615 1831 2133 4482 4738 917
Size: 665 33 216 302 2349 256 1308
Fragments arranged by size:
2349 1308 665 302 256 216 33
AseI AT'TA_AT
Cuts at: 20 508 20
Size: 488 4641
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 1028 2149 1028
Size: 1121 4008
BamHI G'GATC_C
Cuts at: 167 2154 167
Size: 1987 3142
BanI G'GyrC_C
Cuts at: 954 1024 1891 2145 2559 2807 2842 954
Size: 70 867 254 414 248 35 3241
Fragments arranged by size:
3241 867 414 254 248 70 35
BanII G_rGCy'C
Cuts at: 212 966 2143 3173 212
Size: 754 1177 1030 2168
Fragments arranged by size:
2168 1177 1030 754
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 511 938 511
Size: 427 4702
BciVI GTATCCnnnnn_n'
Cuts at: 286 393 3027 4083 5129 286
Size: 107 2634 1056 1046 286
Fragments arranged by size:
2634 1056 1046 286 107
BclI T'GATC_A
Cuts at: 2649 2649
Size: 5129
BfrBI ATG'CAT
Cuts at: 856 1107 856
Size: 251 4878
BglII A'GATC_T
Cuts at: 1647 2644 4681 1647
Size: 997 2037 2095
Fragments arranged by size:
2095 2037 997
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 212 966 1029 2754 2847 3971 212
Size: 754 63 1725 93 1124 1370
Fragments arranged by size:
1725 1370 1124 754 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 2341 2422 2757 4901 2341
Size: 81 335 2144 2569
Fragments arranged by size:
2569 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1745 3496 1745
Size: 1751 3378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 745 971 163
Size: 582 226 4321
Fragments arranged by size:
4321 582 226
Bpu10I CC'TnA_GC
Cuts at: 217 217
Size: 5129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 2305 3184 3898 4196 608
Size: 1697 879 714 298 1541
Fragments arranged by size:
1697 1541 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1391 4833 1391
Size: 3442 1687
BsaAI yAC'GTr
Cuts at: 2482 2482
Size: 5129
BsaBI GATnn'nnATC
Cuts at: 6 1652 1786 2643 4680 6
Size: 1646 134 857 2037 455
Fragments arranged by size:
2037 1646 857 455 134
BsaHI Gr'CG_yC
Cuts at: 2560 2808 3510 5006 2560
Size: 248 702 1496 2683
Fragments arranged by size:
2683 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1999 2318 2839 3517 3928 4075 4349 1999
Size: 319 521 678 411 147 274 2779
Fragments arranged by size:
2779 678 521 411 319 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 380 380
Size: 5129
BseYI C'CCAG_C
Cuts at: 779 2554 3312 3977 4385 779
Size: 1775 758 665 408 1523
Fragments arranged by size:
1775 1523 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 5129
BsiEI CG_ry'CG
Cuts at: 104 291 2671 2717 3947 104
Size: 187 2380 46 1230 1286
Fragments arranged by size:
2380 1286 1230 187 46
BsiHKAI G_wGCw'C
Cuts at: 161 190 2143 2921 3111 3971 161
Size: 29 1953 778 190 860 1319
Fragments arranged by size:
1953 1319 860 778 190 29
Bsp1286I G_dGCh'C
Cuts at: 161 190 212 966 1029 2143 2754 2847 2921
Size: 29 22 754 63 1114 611 93 74
Cuts at: 2921 3111 3173 3971 161
Size: 190 62 798 1319
Fragments arranged by size:
1319 1114 798 754 611 190 93 74
63 62 29 22
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 5129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 626 1423 2179 2393 2695 3076 626
Size: 797 756 214 302 381 2679
Fragments arranged by size:
2679 797 756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 114 165 3421 3475 4959 5124 50
Size: 64 51 3256 54 1484 165 55
Fragments arranged by size:
3256 1484 165 64 55 54 51
BsrDI GCAATG_nn'
Cuts at: 452 2042 3041 452
Size: 1590 999 2540
Fragments arranged by size:
2540 1590 999
BsrFI r'CCGG_y
Cuts at: 3127 3308 3127
Size: 181 4948
BsrGI T'GTAC_A
Cuts at: 536 1499 536
Size: 963 4166
BssSI C'ACGA_G
Cuts at: 160 3400 4108 160
Size: 3240 708 1181
Fragments arranged by size:
3240 1181 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2506 2506
Size: 5129
BstBI TT'CG_AA
Cuts at: 3490 3490
Size: 5129
BstYI r'GATC_y
Cuts at: 1 167 1647 2154 2621 2644 2979 3225 3531
Size: 166 1480 507 467 23 335 246 306
Cuts at: 3531 3543 3629 4681 1
Size: 12 86 1052 449
Fragments arranged by size:
1480 1052 507 467 449 335 306 246
166 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1236 3079 3260 3423 1236
Size: 1843 181 163 2942
Fragments arranged by size:
2942 1843 181 163
BtsI GCAGTG_nn'
Cuts at: 469 2372 469
Size: 1903 3226
ClaI AT'CG_AT
Cuts at: 639 996 639
Size: 357 4772
DraI TTT'AAA
Cuts at: 2128 2128
Size: 5129
DrdI GACnn_nn'nnGTC
Cuts at: 2835 4179 2835
Size: 1344 3785
EaeI y'GGCC_r
Cuts at: 2714 2888 3279 3306 4651 2714
Size: 174 391 27 1345 3192
Fragments arranged by size:
3192 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2714 2714
Size: 5129
EarI CTCTTCn'nnn_
Cuts at: 446 944 1487 1925 3152 3362 446
Size: 498 543 438 1227 210 2213
Fragments arranged by size:
2213 1227 543 498 438 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 148 3337 4079 4225 4939 142
Size: 6 3189 742 146 714 332
Fragments arranged by size:
3189 742 714 332 146 6
EcoICRI GAG'CTC
Cuts at: 2141 2141
Size: 5129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 478 745 971 1327 1654 1688 1946 2954
Size: 315 267 226 356 327 34 258 1008
Cuts at: 2954 3386 3739 4401 163
Size: 432 353 662 891
Fragments arranged by size:
1008 891 662 432 356 353 327 315
267 258 226 34
EcoO109I rG'GnC_Cy
Cuts at: 962 2268 962
Size: 1306 3823
EcoRI G'AATT_C
Cuts at: 917 2133 917
Size: 1216 3913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 5129
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 2563 2811 4041 4812 2563
Size: 248 1230 771 2880
Fragments arranged by size:
2880 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2971 3001 3003 3033 2971
Size: 30 2 30 5067
Fragments arranged by size:
5067 30 30 2
HincII GTy'rAC
Cuts at: 469 2168 469
Size: 1699 3430
HindIII A'AGCT_T
Cuts at: 262 673 262
Size: 411 4718
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 958 2149 958
Size: 1191 3938
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3888 4072 3888
Size: 184 4945
MscI TGG'CCA
Cuts at: 2890 2890
Size: 5129
MslI CAynn'nnrTG
Cuts at: 1852 3118 3245 1852
Size: 1266 127 3736
Fragments arranged by size:
3736 1266 127
MspA1I CmG'CkG
Cuts at: 151 1924 1977 2240 2554 2914 3698 3943 151
Size: 1773 53 263 314 360 784 245 1337
Fragments arranged by size:
1773 1337 784 360 314 263 245 53
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5129
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NcoI C'CATG_G
Cuts at: 107 107
Size: 5129
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5129
NsiI A_TGCA'T
Cuts at: 858 1109 858
Size: 251 4878
NspI r_CATG'y
Cuts at: 1107 1383 1921 2182 2682 4285 1107
Size: 276 538 261 500 1603 1951
Fragments arranged by size:
1951 1603 538 500 276 261
PasI CC'CwG_GG
Cuts at: 1191 1191
Size: 5129
PciI A'CATG_T
Cuts at: 4281 4281
Size: 5129
PflMI CCAn_nnn'nTGG
Cuts at: 972 972
Size: 5129
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 5097
PspOMI G'GGCC_C
Cuts at: 208 962 208
Size: 754 4375
PstI C_TGCA'G
Cuts at: 1736 2176 2861 1736
Size: 440 685 4004
Fragments arranged by size:
4004 685 440
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 1924 1977 2554 2914 1924
Size: 53 577 360 4139
Fragments arranged by size:
4139 577 360 53
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 2143 2143
Size: 5129
SalI G'TCGA_C
Cuts at: 467 2166 467
Size: 1699 3430
SapI GCTCTTCn'nnn_
Cuts at: 446 3152 3362 446
Size: 2706 210 2213
Fragments arranged by size:
2706 2213 210
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 888 1822 4646 888
Size: 934 2824 1371
Fragments arranged by size:
2824 1371 934
SexAI A'CCwGG_T
Cuts at: 2030 2030
Size: 5129
SfcI C'TryA_G
Cuts at: 33 1125 1467 1732 1972 2172 2857 3825 4016
Size: 1092 342 265 240 200 685 968 191
Cuts at: 4016 33
Size: 1146
Fragments arranged by size:
1146 1092 968 685 342 265 240 200
191
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 1030 2151 1030
Size: 1121 4008
SmlI C'TyrA_G
Cuts at: 623 2284 3199 3913 4175 623
Size: 1661 915 714 262 1577
Fragments arranged by size:
1661 1577 915 714 262
SpeI A'CTAG_T
Cuts at: 67 889 67
Size: 822 4307
SphI G_CATG'C
Cuts at: 2182 2682 2182
Size: 500 4629
SspI AAT'ATT
Cuts at: 486 1758 486
Size: 1272 3857
StyI C'CwwG_G
Cuts at: 107 2263 2617 107
Size: 2156 354 2619
Fragments arranged by size:
2619 2156 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 305 1012 2712 2845 3304 3513 305
Size: 707 1700 133 459 209 1921
Fragments arranged by size:
1921 1700 707 459 209 133
TatI w'GTAC_w
Cuts at: 536 886 1499 1820 4644 536
Size: 350 613 321 2824 1021
Fragments arranged by size:
2824 1021 613 350 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 4646 4678 2067
Size: 32 2547 32 2518
Fragments arranged by size:
2547 2518 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 603 2160 603
Size: 1557 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2624 2624
Size: 5129
XmaI C'CCGG_G
Cuts at: 1028 2149 1028
Size: 1121 4008
XmnI GAAnn'nnTTC
Cuts at: 486 1649 486
Size: 1163 3966
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI