Restriction map for
pF1KA0911
(Circular) (Six-base) MAPSORT of: pF1KA0911.seq Check: 7186 from: 1 to: 6056
pF1KA0911 6056 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 895 1672 2017 2435 895
Size: 777 345 418 4516
Fragments arranged by size:
4516 777 418 345
AccI GT'mk_AC
Cuts at: 1783 3094 1783
Size: 1311 4745
Acc65I G'GTAC_C
Cuts at: 3072 3072
Size: 6056
AclI AA'CG_TT
Cuts at: 5896 5896
Size: 6056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 3881 4313 4666 5328 2210
Size: 1671 432 353 662 2938
Fragments arranged by size:
2938 1671 662 432 353
AflIII A'CryG_T
Cuts at: 979 3408 5208 5489 979
Size: 2429 1800 281 1546
Fragments arranged by size:
2429 1800 1546 281
AleI CACnn'nnGTG
Cuts at: 529 2389 2876 529
Size: 1860 487 3709
Fragments arranged by size:
3709 1860 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1852 1852
Size: 6056
AlwNI CAG_nnn'CTG
Cuts at: 2110 3021 3361 4799 2110
Size: 911 340 1438 3367
Fragments arranged by size:
3367 1438 911 340
ApaLI G'TGCA_C
Cuts at: 2248 2602 4894 2248
Size: 354 2292 3410
Fragments arranged by size:
3410 2292 354
ApoI r'AATT_y
Cuts at: 371 1504 1958 2113 2521 3060 5409 5665 371
Size: 1133 454 155 408 539 2349 256 762
Fragments arranged by size:
2349 1133 762 539 454 408 256 155
AseI AT'TA_AT
Cuts at: 20 20
Size: 6056
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6056
AvaI C'yCGr_G
Cuts at: 1128 1509 1891 2056 2099 3076 1128
Size: 381 382 165 43 977 4108
Fragments arranged by size:
4108 977 382 381 165 43
BamHI G'GATC_C
Cuts at: 1135 1223 1407 2750 3081 1135
Size: 88 184 1343 331 4110
Fragments arranged by size:
4110 1343 331 184 88
BanI G'GyrC_C
Cuts at: 523 937 1207 1352 1379 1481 3072 3486 3734
Size: 414 270 145 27 102 1591 414 248
Cuts at: 3734 3769 523
Size: 35 2810
Fragments arranged by size:
2810 1591 414 414 270 248 145 102
35 27
BanII G_rGCy'C
Cuts at: 227 1550 2066 3070 4100 227
Size: 1323 516 1004 1030 2183
Fragments arranged by size:
2183 1323 1030 1004 516
BbeI G_GCGC'C
Cuts at: 3490 3738 3490
Size: 248 5808
BbsI GAAGACnn'nnnn_
Cuts at: 1826 1892 1826
Size: 66 5990
BbvCI CC'TCA_GC
Cuts at: 2204 3043 2204
Size: 839 5217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6022
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3954 5010 6056 2253
Size: 1701 1056 1046 2253
Fragments arranged by size:
2253 1701 1056 1046
BclI T'GATC_A
Cuts at: 3576 3576
Size: 6056
BglI GCCn_nnn'nGGC
Cuts at: 2081 2081
Size: 6056
BglII A'GATC_T
Cuts at: 3571 5608 3571
Size: 2037 4019
BlpI GC'TnA_GC
Cuts at: 3168 3168
Size: 6056
Bme1580I G_kGCm'C
Cuts at: 737 940 1108 2252 2606 3681 3774 4898 737
Size: 203 168 1144 354 1075 93 1124 1895
Fragments arranged by size:
1895 1144 1124 1075 354 203 168 93
BmgBI CAC'GTC
Cuts at: 576 732 1538 576
Size: 156 806 5094
Fragments arranged by size:
5094 806 156
BmrI ACTGGGnnnn_n'
Cuts at: 1698 2380 2823 3268 3349 3684 5828 1698
Size: 682 443 445 81 335 2144 1926
Fragments arranged by size:
2144 1926 682 445 443 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1535 1569 1906 4423 1535
Size: 34 337 2517 3168
Fragments arranged by size:
3168 2517 337 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 710 994 2335 3043 241
Size: 469 284 1341 708 3254
Fragments arranged by size:
3254 1341 708 469 284
Bpu10I CC'TnA_GC
Cuts at: 2147 2204 3043 2147
Size: 57 839 5160
Fragments arranged by size:
5160 839 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3232 4111 4825 5123 3232
Size: 879 714 298 4165
Fragments arranged by size:
4165 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2848 5760 2848
Size: 2912 3144
BsaAI yAC'GTr
Cuts at: 2040 3409 2040
Size: 1369 4687
BsaBI GATnn'nnATC
Cuts at: 6 1346 3570 5607 6
Size: 1340 2224 2037 455
Fragments arranged by size:
2224 2037 1340 455
BsaHI Gr'CG_yC
Cuts at: 1235 2084 3487 3735 4437 5933 1235
Size: 849 1403 248 702 1496 1358
Fragments arranged by size:
1496 1403 1358 849 702 248
BsaWI w'CCGG_w
Cuts at: 2468 3245 3766 4444 4855 5002 5276 2468
Size: 777 521 678 411 147 274 3248
Fragments arranged by size:
3248 777 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1345 1375 1918 1948 1345
Size: 30 543 30 5453
Fragments arranged by size:
5453 543 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 1432 2074 2254 2302 2428 2896 2899 2902
Size: 1173 642 180 48 126 468 3 3
Cuts at: 2902 2983 2986 2989 259
Size: 81 3 3 3326
Fragments arranged by size:
3326 1173 642 468 180 126 81 48
3 3 3 3
BseYI C'CCAG_C
Cuts at: 1855 2588 2677 3481 4239 4904 5312 1855
Size: 733 89 804 758 665 408 2599
Fragments arranged by size:
2599 804 758 733 665 408 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1678 1858 873
Size: 805 180 5071
Fragments arranged by size:
5071 805 180
BsiEI CG_ry'CG
Cuts at: 104 143 1053 1100 3598 3644 4874 104
Size: 39 910 47 2498 46 1230 1286
Fragments arranged by size:
2498 1286 1230 910 47 46 39
BsiHKAI G_wGCw'C
Cuts at: 269 2252 2606 3070 3848 4038 4898 269
Size: 1983 354 464 778 190 860 1427
Fragments arranged by size:
1983 1427 860 778 464 354 190
Bsp1286I G_dGCh'C
Cuts at: 227 269 737 940 1108 1550 2066 2252 2606
Size: 42 468 203 168 442 516 186 354
Cuts at: 2606 3070 3681 3774 3848 4038 4100 4898 227
Size: 464 611 93 74 190 62 798 1385
Fragments arranged by size:
1385 798 611 516 468 464 442 354
203 190 186 168 93 74 62 42
BspHI T'CATG_A
Cuts at: 2726 4488 2726
Size: 1762 4294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 895 1443 1672 1700 1795 1800 1814 2017 2435
Size: 548 229 28 95 5 14 203 418
Cuts at: 2435 3106 3320 3622 4003 895
Size: 671 214 302 381 2948
Fragments arranged by size:
2948 671 548 418 381 302 229 214
203 95 28 14 5
BsrBI CCG'CTC
Cuts at: 50 124 1575 2618 4348 4402 5886 6051 50
Size: 74 1451 1043 1730 54 1484 165 55
Fragments arranged by size:
1730 1484 1451 1043 165 74 55 54
BsrDI GCAATG_nn'
Cuts at: 3968 3968
Size: 6056
BsrFI r'CCGG_y
Cuts at: 940 1082 1382 4054 4235 940
Size: 142 300 2672 181 2761
Fragments arranged by size:
2761 2672 300 181 142
BssSI C'ACGA_G
Cuts at: 1543 1603 2172 2299 4327 5035 1543
Size: 60 569 127 2028 708 2564
Fragments arranged by size:
2564 2028 708 569 127 60
BstAPI GCAn_nnn'nTGC
Cuts at: 2430 3433 2430
Size: 1003 5053
BstBI TT'CG_AA
Cuts at: 4417 4417
Size: 6056
BstXI CCAn_nnnn'nTGG
Cuts at: 1863 2562 2580 1863
Size: 699 18 5339
Fragments arranged by size:
5339 699 18
BstYI r'GATC_y
Cuts at: 1 1135 1223 1407 1841 2750 3081 3548 3571
Size: 1134 88 184 434 909 331 467 23
Cuts at: 3571 3906 4152 4458 4470 4556 5608 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1134 1052 909 467 449 434 335 331
306 246 184 88 86 23 12
BstZ17I GTA'TAC
Cuts at: 1784 1784
Size: 6056
BtgI C'CryG_G
Cuts at: 234 620 668 1100 1151 1374 2399 2561 2849
Size: 386 48 432 51 223 1025 162 288
Cuts at: 2849 234
Size: 3441
Fragments arranged by size:
3441 1025 432 386 288 223 162 51
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4006 4187 4350 4006
Size: 181 163 5712
Fragments arranged by size:
5712 181 163
BtsI GCAGTG_nn'
Cuts at: 407 818 1106 1223 2681 2883 3299 407
Size: 411 288 117 1458 202 416 3164
Fragments arranged by size:
3164 1458 416 411 288 202 117
DraI TTT'AAA
Cuts at: 369 3055 369
Size: 2686 3370
DrdI GACnn_nn'nnGTC
Cuts at: 991 1810 3762 5106 991
Size: 819 1952 1344 1941
Fragments arranged by size:
1952 1941 1344 819
EaeI y'GGCC_r
Cuts at: 140 158 950 1097 1173 2402 2502 2552 2648
Size: 18 792 147 76 1229 100 50 96
Cuts at: 2648 3641 3815 4206 4233 5578 140
Size: 993 174 391 27 1345 618
Fragments arranged by size:
1345 1229 993 792 618 391 174 147
100 96 76 50 27 18
EagI C'GGCC_G
Cuts at: 140 1097 3641 140
Size: 957 2544 2555
Fragments arranged by size:
2555 2544 957
EarI CTCTTCn'nnn_
Cuts at: 1402 2888 2894 2900 2924 4079 4289 1402
Size: 1486 6 6 24 1155 210 3169
Fragments arranged by size:
3169 1486 1155 210 24 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2784 4264 5006 5152 5866 2784
Size: 1480 742 146 714 2974
Fragments arranged by size:
2974 1480 742 714 146
EcoICRI GAG'CTC
Cuts at: 3068 3068
Size: 6056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 710 994 2210 2335 3043 3881 4313 4666
Size: 469 284 1216 125 708 838 432 353
Cuts at: 4666 5328 241
Size: 662 969
Fragments arranged by size:
1216 969 838 708 662 469 432 353
284 125
EcoO109I rG'GnC_Cy
Cuts at: 514 1812 2208 2456 3195 514
Size: 1298 396 248 739 3375
Fragments arranged by size:
3375 1298 739 396 248
EcoRI G'AATT_C
Cuts at: 1504 1958 3060 1504
Size: 454 1102 4500
Fragments arranged by size:
4500 1102 454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4075 4075
Size: 6056
FspI TGC'GCA
Cuts at: 3837 3837
Size: 6056
HaeII r_GCGC'y
Cuts at: 2951 3490 3738 4968 5739 2951
Size: 539 248 1230 771 3268
Fragments arranged by size:
3268 1230 771 539 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1048 1080 1522 1554 1581 1613 1761 1793 1958
Size: 32 442 32 27 32 148 32 165
Cuts at: 1958 1990 3898 3928 3930 3960 1048
Size: 32 1908 30 2 30 3144
Fragments arranged by size:
3144 1908 442 165 148 32 32 32
32 32 30 30 27 2
HincII GTy'rAC
Cuts at: 201 747 2628 2844 3095 201
Size: 546 1881 216 251 3162
Fragments arranged by size:
3162 1881 546 251 216
HpaI GTT'AAC
Cuts at: 201 201
Size: 6056
KasI G'GCGC_C
Cuts at: 3486 3734 3486
Size: 248 5808
KpnI G_GTAC'C
Cuts at: 3076 3076
Size: 6056
MluI A'CGCG_T
Cuts at: 5489 5489
Size: 6056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2936 4815 4999 2936
Size: 1879 184 3993
Fragments arranged by size:
3993 1879 184
MscI TGG'CCA
Cuts at: 1175 2404 2650 3817 1175
Size: 1229 246 1167 3414
Fragments arranged by size:
3414 1229 1167 246
MslI CAynn'nnrTG
Cuts at: 529 1273 2353 2389 2876 4045 4172 529
Size: 744 1080 36 487 1169 127 2413
Fragments arranged by size:
2413 1169 1080 744 487 127 36
MspA1I CmG'CkG
Cuts at: 1102 3021 3167 3481 3841 4625 4870 1102
Size: 1919 146 314 360 784 245 2288
Fragments arranged by size:
2288 1919 784 360 314 245 146
NaeI GCC'GGC
Cuts at: 1384 4237 1384
Size: 2853 3203
NarI GG'CG_CC
Cuts at: 3487 3735 3487
Size: 248 5808
NcoI C'CATG_G
Cuts at: 1151 2399 2561 2849 1151
Size: 1248 162 288 4358
Fragments arranged by size:
4358 1248 288 162
NgoMIV G'CCGG_C
Cuts at: 1382 4235 1382
Size: 2853 3203
NotI GC'GGCC_GC
Cuts at: 1097 1097
Size: 6056
NspI r_CATG'y
Cuts at: 983 2452 3109 3609 5212 983
Size: 1469 657 500 1603 1827
Fragments arranged by size:
1827 1603 1469 657 500
PasI CC'CwG_GG
Cuts at: 895 895
Size: 6056
PciI A'CATG_T
Cuts at: 979 5208 979
Size: 4229 1827
PflMI CCAn_nnn'nTGG
Cuts at: 2646 2646
Size: 6056
PfoI T'CCnGG_A
Cuts at: 1226 1744 1226
Size: 518 5538
PmeI GTTT'AAAC
Cuts at: 3055 3055
Size: 6056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 4502
Size: 32 6024
PpuMI rG'GwC_Cy
Cuts at: 514 1812 2208 2456 514
Size: 1298 396 248 4114
Fragments arranged by size:
4114 1298 396 248
PstI C_TGCA'G
Cuts at: 470 1440 1683 1811 2339 3103 3788 470
Size: 970 243 128 528 764 685 2738
Fragments arranged by size:
2738 970 764 685 528 243 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6056
PvuII CAG'CTG
Cuts at: 3021 3481 3841 3021
Size: 460 360 5236
Fragments arranged by size:
5236 460 360
RsrII CG'GwC_CG
Cuts at: 4251 4251
Size: 6056
SacI G_AGCT'C
Cuts at: 3070 3070
Size: 6056
SacII CC_GC'GG
Cuts at: 1103 1103
Size: 6056
SalI G'TCGA_C
Cuts at: 3093 3093
Size: 6056
SapI GCTCTTCn'nnn_
Cuts at: 4079 4289 4079
Size: 210 5846
SbfI CC_TGCA'GG
Cuts at: 1811 3103 1811
Size: 1292 4764
ScaI AGT'ACT
Cuts at: 5573 5573
Size: 6056
SexAI A'CCwGG_T
Cuts at: 1187 1187
Size: 6056
SfcI C'TryA_G
Cuts at: 33 466 1436 1679 1807 2335 3099 3784 4752
Size: 433 970 243 128 528 764 685 968
Cuts at: 4752 4943 33
Size: 191 1146
Fragments arranged by size:
1146 970 968 764 685 528 433 243
191 128
SfoI GGC'GCC
Cuts at: 3488 3736 3488
Size: 248 5808
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6056
SgrAI Cr'CCGG_yG
Cuts at: 940 940
Size: 6056
SmaI CCC'GGG
Cuts at: 3078 3078
Size: 6056
SmlI C'TyrA_G
Cuts at: 3211 4126 4840 5102 3211
Size: 915 714 262 4165
Fragments arranged by size:
4165 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6056
SphI G_CATG'C
Cuts at: 2452 3109 3609 2452
Size: 657 500 4899
Fragments arranged by size:
4899 657 500
StuI AGG'CCT
Cuts at: 496 496
Size: 6056
StyI C'CwwG_G
Cuts at: 1151 1874 2399 2561 2849 3190 3544 1151
Size: 723 525 162 288 341 354 3663
Fragments arranged by size:
3663 723 525 354 341 288 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 887 1067 1225 1233 2007 3639 3772 4231 4440
Size: 180 158 8 774 1632 133 459 209
Cuts at: 4440 887
Size: 2503
Fragments arranged by size:
2503 1632 774 459 209 180 158 133
8
TatI w'GTAC_w
Cuts at: 1002 5571 1002
Size: 4569 1487
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1345 1377 5573 5605 1345
Size: 32 4196 32 1796
Fragments arranged by size:
4196 1796 32 32
Tth111I GACn'n_nGTC
Cuts at: 3853 3853
Size: 6056
XbaI T'CTAG_A
Cuts at: 3087 3087
Size: 6056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 1087 3551 450
Size: 637 2464 2955
Fragments arranged by size:
2955 2464 637
XmaI C'CCGG_G
Cuts at: 3076 3076
Size: 6056
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BfrBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI