Restriction map for
pF1KA0909
(Circular) (Six-base) MAPSORT of: pF1KA0909.seq Check: 1817 from: 1 to: 6719
pF1KA0909 6719 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1399 1533 1890 2076 1399
Size: 134 357 186 6042
Fragments arranged by size:
6042 357 186 134
AccI GT'mk_AC
Cuts at: 3757 3757
Size: 6719
Acc65I G'GTAC_C
Cuts at: 3735 3735
Size: 6719
AclI AA'CG_TT
Cuts at: 6559 6559
Size: 6719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 448 649 682 1593 2840 3134 3328 3685
Size: 273 201 33 911 1247 294 194 357
Cuts at: 3685 4544 4976 5723 5991 175
Size: 859 432 747 268 903
Fragments arranged by size:
1247 911 903 859 747 432 357 294
273 268 201 194 33
AfeI AGC'GCT
Cuts at: 3346 3346
Size: 6719
AflII C'TTAA_G
Cuts at: 3385 3385
Size: 6719
AflIII A'CryG_T
Cuts at: 4071 5175 6152 4071
Size: 1104 977 4638
Fragments arranged by size:
4638 1104 977
AgeI A'CCGG_T
Cuts at: 5107 5107
Size: 6719
AhdI GACnn_n'nnGTC
Cuts at: 1995 1995
Size: 6719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2276 2276
Size: 6719
AlwNI CAG_nnn'CTG
Cuts at: 2080 2454 2608 2722 3031 3334 3681 4024 5591
Size: 374 154 114 309 303 347 343 1567
Cuts at: 5591 2080
Size: 3208
Fragments arranged by size:
3208 1567 374 347 343 309 303 154
114
ApaI G_GGCC'C
Cuts at: 1922 2825 1922
Size: 903 5816
ApaLI G'TGCA_C
Cuts at: 538 5489 538
Size: 4951 1768
ApoI r'AATT_y
Cuts at: 3723 6072 6328 3723
Size: 2349 256 4114
Fragments arranged by size:
4114 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6719
AvaI C'yCGr_G
Cuts at: 407 638 875 1049 1309 1382 2504 2995 3263
Size: 231 237 174 260 73 1122 491 268
Cuts at: 3263 3739 407
Size: 476 3387
Fragments arranged by size:
3387 1122 491 476 268 260 237 231
174 73
AvrII C'CTAG_G
Cuts at: 1156 1156
Size: 6719
BamHI G'GATC_C
Cuts at: 3744 3744
Size: 6719
BanI G'GyrC_C
Cuts at: 2088 2650 3735 4149 4397 4432 2088
Size: 562 1085 414 248 35 4375
Fragments arranged by size:
4375 1085 562 414 248 35
BanII G_rGCy'C
Cuts at: 875 956 1019 1370 1466 1607 1922 1956 2570
Size: 81 63 351 96 141 315 34 614
Cuts at: 2570 2825 3041 3733 4763 875
Size: 255 216 692 1030 2831
Fragments arranged by size:
2831 1030 692 614 351 315 255 216
141 96 81 63 34
BbeI G_GCGC'C
Cuts at: 4153 4401 4153
Size: 248 6471
BbsI GAAGACnn'nnnn_
Cuts at: 181 406 181
Size: 225 6494
BciVI GTATCCnnnnn_n'
Cuts at: 4617 5378 6719 4617
Size: 761 1341 4617
Fragments arranged by size:
4617 1341 761
BclI T'GATC_A
Cuts at: 260 1812 4239 260
Size: 1552 2427 2740
Fragments arranged by size:
2740 2427 1552
BglI GCCn_nnn'nGGC
Cuts at: 1131 1131
Size: 6719
BglII A'GATC_T
Cuts at: 158 4234 6271 158
Size: 4076 2037 606
Fragments arranged by size:
4076 2037 606
BlpI GC'TnA_GC
Cuts at: 3831 3831
Size: 6719
Bme1580I G_kGCm'C
Cuts at: 300 542 1922 2403 2685 2825 4344 4437 5493
Size: 242 1380 481 282 140 1519 93 1056
Cuts at: 5493 300
Size: 1526
Fragments arranged by size:
1526 1519 1380 1056 481 282 242 140
93
BmgBI CAC'GTC
Cuts at: 2666 2666
Size: 6719
BmrI ACTGGGnnnn_n'
Cuts at: 1214 3931 4012 4347 6491 1214
Size: 2717 81 335 2144 1442
Fragments arranged by size:
2717 2144 1442 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1815 3192 5086 1815
Size: 1377 1894 3448
Fragments arranged by size:
3448 1894 1377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 202 499 583 1120 1133 1384 1621 2062
Size: 5 297 84 537 13 251 237 441
Cuts at: 2062 2099 2580 2858 3023 3094 197
Size: 37 481 278 165 71 3822
Fragments arranged by size:
3822 537 481 441 297 278 251 237
165 84 71 37 13 5
Bpu10I CC'TnA_GC
Cuts at: 2296 2296
Size: 6719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2554 3895 4774 5266 5564 1450
Size: 1104 1341 879 492 298 2605
Fragments arranged by size:
2605 1341 1104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2284 3040 6423 2284
Size: 756 3383 2580
Fragments arranged by size:
3383 2580 756
BsaAI yAC'GTr
Cuts at: 4072 4072
Size: 6719
BsaBI GATnn'nnATC
Cuts at: 6 4233 6270 6
Size: 4227 2037 455
Fragments arranged by size:
4227 2037 455
BsaHI Gr'CG_yC
Cuts at: 1123 4150 4398 5100 6596 1123
Size: 3027 248 702 1496 1246
Fragments arranged by size:
3027 1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 2018 2958 3353 3545 3908 4429 5107 5381 5528
Size: 940 395 192 363 521 678 274 147
Cuts at: 5528 5939 2018
Size: 411 2798
Fragments arranged by size:
2798 940 678 521 411 395 363 274
192 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 634 664 1568 1598 3499 3529 3565 3595 634
Size: 30 904 30 1901 30 36 30 3758
Fragments arranged by size:
3758 1901 904 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 658 914 987 1075 1082 1205 1474 1574
Size: 402 256 73 88 7 123 269 100
Cuts at: 1574 2102 2583 2616 2779 2820 256
Size: 528 481 33 163 41 4155
Fragments arranged by size:
4155 528 481 402 269 256 163 123
100 88 73 41 33 7
BseYI C'CCAG_C
Cuts at: 555 1685 2080 3094 3109 3695 4144 4902 5479
Size: 1130 395 1014 15 586 449 758 577
Cuts at: 5479 5975 555
Size: 496 1299
Fragments arranged by size:
1299 1130 1014 758 586 577 496 449
395 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 2236 2690 754
Size: 1482 454 4783
Fragments arranged by size:
4783 1482 454
BsiEI CG_ry'CG
Cuts at: 104 615 4261 4307 5515 104
Size: 511 3646 46 1208 1308
Fragments arranged by size:
3646 1308 1208 511 46
BsiHKAI G_wGCw'C
Cuts at: 542 956 1466 1763 1940 2429 3497 3733 4511
Size: 414 510 297 177 489 1068 236 778
Cuts at: 4511 4701 5493 542
Size: 190 792 1768
Fragments arranged by size:
1768 1068 792 778 510 489 414 297
236 190 177
BsmI GAATG_Cn'
Cuts at: 2459 2459
Size: 6719
BsmBI CGTCTCn'nnnn_
Cuts at: 2628 2628
Size: 6719
Bsp1286I G_dGCh'C
Cuts at: 300 542 875 956 1019 1370 1466 1607 1763
Size: 242 333 81 63 351 96 141 156
Cuts at: 1763 1922 1940 1956 2403 2429 2570 2685 2825
Size: 159 18 16 447 26 141 115 140
Cuts at: 2825 3041 3497 3733 4344 4437 4511 4701 4763
Size: 216 456 236 611 93 74 190 62
Cuts at: 4763 5493 300
Size: 730 1526
Fragments arranged by size:
1526 730 611 456 447 351 333 242
236 216 190 159 156 141 141 140
115 96 93 81 74 63 62 26
18 16
BspEI T'CCGG_A
Cuts at: 2018 5939 2018
Size: 3921 2798
BspHI T'CATG_A
Cuts at: 3623 5895 3623
Size: 2272 4447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 1533 1890 2076 3769 3983 4285 4666 1399
Size: 134 357 186 1693 214 302 381 3452
Fragments arranged by size:
3452 1693 381 357 302 214 186 134
BsrBI CCG'CTC
Cuts at: 50 1324 2355 2964 3021 3487 3515 5011 5065
Size: 1274 1031 609 57 466 28 1496 54
Cuts at: 5065 6549 6714 50
Size: 1484 165 55
Fragments arranged by size:
1496 1484 1274 1031 609 466 165 57
55 54 28
BsrDI GCAATG_nn'
Cuts at: 4631 4631
Size: 6719
BsrFI r'CCGG_y
Cuts at: 3299 4717 4898 5107 3299
Size: 1418 181 209 4911
Fragments arranged by size:
4911 1418 209 181
BssSI C'ACGA_G
Cuts at: 3235 4990 5348 3235
Size: 1755 358 4606
Fragments arranged by size:
4606 1755 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4096 4096
Size: 6719
BstBI TT'CG_AA
Cuts at: 2147 5080 2147
Size: 2933 3786
BstEII G'GTnAC_C
Cuts at: 370 370
Size: 6719
BstXI CCAn_nnnn'nTGG
Cuts at: 1137 1173 1276 1599 2140 3072 1137
Size: 36 103 323 541 932 4784
Fragments arranged by size:
4784 932 541 323 103 36
BstYI r'GATC_y
Cuts at: 1 158 3744 4211 4234 4569 4815 5827 5913
Size: 157 3586 467 23 335 246 1012 86
Cuts at: 5913 5925 6271 1
Size: 12 346 449
Fragments arranged by size:
3586 1012 467 449 346 335 246 157
86 23 12
BtgI C'CryG_G
Cuts at: 2220 2220
Size: 6719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1803 4669 4850 5013 1803
Size: 2866 181 163 3509
Fragments arranged by size:
3509 2866 181 163
BtsI GCAGTG_nn'
Cuts at: 801 1073 1184 1829 3962 801
Size: 272 111 645 2133 3558
Fragments arranged by size:
3558 2133 645 272 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 114 149 114
Size: 35 6684
DraI TTT'AAA
Cuts at: 3718 3718
Size: 6719
DraIII CAC_nnn'GTG
Cuts at: 732 732
Size: 6719
DrdI GACnn_nn'nnGTC
Cuts at: 4425 5283 4425
Size: 858 5861
EaeI y'GGCC_r
Cuts at: 3062 3419 3554 3707 4304 4478 4869 4896 6241
Size: 357 135 153 597 174 391 27 1345
Cuts at: 6241 3062
Size: 3540
Fragments arranged by size:
3540 1345 597 391 357 174 153 135
27
EagI C'GGCC_G
Cuts at: 4304 4304
Size: 6719
EarI CTCTTCn'nnn_
Cuts at: 477 704 993 1413 1551 4742 4952 477
Size: 227 289 420 138 3191 210 2244
Fragments arranged by size:
3191 2244 420 289 227 210 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2322 4927 5237 5383 6529 2322
Size: 2605 310 146 1146 2512
Fragments arranged by size:
2605 2512 1146 310 146
EcoICRI GAG'CTC
Cuts at: 954 1464 3731 954
Size: 510 2267 3942
Fragments arranged by size:
3942 2267 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 197 202 448 499 583 649 682 1120
Size: 22 5 246 51 84 66 33 438
Cuts at: 1120 1133 1384 1593 1621 2062 2099 2580 2840
Size: 13 251 209 28 441 37 481 260
Cuts at: 2840 2858 3023 3094 3134 3328 3685 4544 4976
Size: 18 165 71 40 194 357 859 432
Cuts at: 4976 5723 5991 175
Size: 747 268 903
Fragments arranged by size:
903 859 747 481 441 438 432 357
268 260 251 246 209 194 165 84
71 66 51 40 37 33 28 22
18 13 5
EcoO109I rG'GnC_Cy
Cuts at: 1387 1579 1771 1918 1919 2101 2137 2203 2821
Size: 192 192 147 1 182 36 66 618
Cuts at: 2821 2822 3858 1387
Size: 1 1036 4248
Fragments arranged by size:
4248 1036 618 192 192 182 147 66
36 1 1
EcoRI G'AATT_C
Cuts at: 3723 3723
Size: 6719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4738 4738
Size: 6719
FspI TGC'GCA
Cuts at: 4500 4500
Size: 6719
HaeII r_GCGC'y
Cuts at: 501 3348 3642 4153 4401 5423 6402 501
Size: 2847 294 511 248 1022 979 818
Fragments arranged by size:
2847 1022 979 818 511 294 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 274 297 306 329 1631 1663 1981
Size: 32 7 23 9 23 1302 32 318
Cuts at: 1981 2013 2356 2388 2911 2943 3037 3069 4561
Size: 32 343 32 523 32 94 32 1492
Cuts at: 4561 4591 4593 4623 235
Size: 30 2 30 2331
Fragments arranged by size:
2331 1492 1302 523 343 318 94 32
32 32 32 32 32 30 30 23
23 9 7 2
HincII GTy'rAC
Cuts at: 2349 3758 2349
Size: 1409 5310
HindIII A'AGCT_T
Cuts at: 2325 2325
Size: 6719
KasI G'GCGC_C
Cuts at: 4149 4397 4149
Size: 248 6471
KpnI G_GTAC'C
Cuts at: 3739 3739
Size: 6719
MluI A'CGCG_T
Cuts at: 6152 6152
Size: 6719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1702 2419 2624 5390 5574 1702
Size: 717 205 2766 184 2847
Fragments arranged by size:
2847 2766 717 205 184
MscI TGG'CCA
Cuts at: 3064 3709 4480 3064
Size: 645 771 5303
Fragments arranged by size:
5303 771 645
MslI CAynn'nnrTG
Cuts at: 1822 4708 4835 1822
Size: 2886 127 3706
Fragments arranged by size:
3706 2886 127
MspA1I CmG'CkG
Cuts at: 204 660 1107 1378 1689 2080 2528 3243 3334
Size: 456 447 271 311 391 448 715 91
Cuts at: 3334 3369 3830 4144 4504 5517 5762 204
Size: 35 461 314 360 1013 245 1161
Fragments arranged by size:
1161 1013 715 461 456 448 447 391
360 314 311 271 245 91 35
NaeI GCC'GGC
Cuts at: 3301 4900 3301
Size: 1599 5120
NarI GG'CG_CC
Cuts at: 4150 4398 4150
Size: 248 6471
NcoI C'CATG_G
Cuts at: 2220 2220
Size: 6719
NdeI CA'TA_TG
Cuts at: 1275 1275
Size: 6719
NgoMIV G'CCGG_C
Cuts at: 3299 4898 3299
Size: 1599 5120
NspI r_CATG'y
Cuts at: 3772 4272 5179 3772
Size: 500 907 5312
Fragments arranged by size:
5312 907 500
PasI CC'CwG_GG
Cuts at: 2268 2403 2268
Size: 135 6584
PciI A'CATG_T
Cuts at: 5175 5175
Size: 6719
PflMI CCAn_nnn'nTGG
Cuts at: 441 1747 2608 3056 3681 441
Size: 1306 861 448 625 3479
Fragments arranged by size:
3479 1306 861 625 448
PmeI GTTT'AAAC
Cuts at: 3718 3718
Size: 6719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5858 5890 5858
Size: 32 6687
PpuMI rG'GwC_Cy
Cuts at: 1387 1579 1771 2101 2203 1387
Size: 192 192 330 102 5903
Fragments arranged by size:
5903 330 192 192 102
PshAI GACnn'nnGTC
Cuts at: 2609 2609
Size: 6719
PspOMI G'GGCC_C
Cuts at: 1918 2821 1918
Size: 903 5816
PstI C_TGCA'G
Cuts at: 586 796 3766 4451 586
Size: 210 2970 685 2854
Fragments arranged by size:
2970 2854 685 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6719
PvuII CAG'CTG
Cuts at: 660 1378 1689 2080 3334 3369 4144 4504 660
Size: 718 311 391 1254 35 775 360 2875
Fragments arranged by size:
2875 1254 775 718 391 360 311 35
RsrII CG'GwC_CG
Cuts at: 4914 4914
Size: 6719
SacI G_AGCT'C
Cuts at: 956 1466 3733 956
Size: 510 2267 3942
Fragments arranged by size:
3942 2267 510
SalI G'TCGA_C
Cuts at: 3756 3756
Size: 6719
SanDI GG'GwC_CC
Cuts at: 1387 1387
Size: 6719
SapI GCTCTTCn'nnn_
Cuts at: 4742 4952 4742
Size: 210 6509
SbfI CC_TGCA'GG
Cuts at: 3766 3766
Size: 6719
ScaI AGT'ACT
Cuts at: 1980 6236 1980
Size: 4256 2463
SexAI A'CCwGG_T
Cuts at: 728 728
Size: 6719
SfcI C'TryA_G
Cuts at: 33 582 719 792 2542 3762 4447 5440 5631
Size: 549 137 73 1750 1220 685 993 191
Cuts at: 5631 33
Size: 1121
Fragments arranged by size:
1750 1220 1121 993 685 549 191 137
73
SfoI GGC'GCC
Cuts at: 4151 4399 4151
Size: 248 6471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6719
SmaI CCC'GGG
Cuts at: 640 2506 3741 640
Size: 1866 1235 3618
Fragments arranged by size:
3618 1866 1235
SmlI C'TyrA_G
Cuts at: 1049 1465 2533 3385 3874 4789 5281 5543 1049
Size: 416 1068 852 489 915 492 262 2225
Fragments arranged by size:
2225 1068 915 852 492 489 416 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6719
SphI G_CATG'C
Cuts at: 3772 4272 3772
Size: 500 6219
StuI AGG'CCT
Cuts at: 312 1161 1567 312
Size: 849 406 5464
Fragments arranged by size:
5464 849 406
StyI C'CwwG_G
Cuts at: 116 959 1156 1532 1774 2220 2816 3853 4207
Size: 843 197 376 242 446 596 1037 354
Cuts at: 4207 116
Size: 2628
Fragments arranged by size:
2628 1037 843 596 446 376 354 242
197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 766 770 1503 1795 4302 4435 4894 5103 766
Size: 4 733 292 2507 133 459 209 2382
Fragments arranged by size:
2507 2382 733 459 292 209 133 4
TatI w'GTAC_w
Cuts at: 1978 3290 3359 6234 1978
Size: 1312 69 2875 2463
Fragments arranged by size:
2875 2463 1312 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3499 3531 6236 6268 3499
Size: 32 2705 32 3950
Fragments arranged by size:
3950 2705 32 32
Tth111I GACn'n_nGTC
Cuts at: 529 3051 4516 529
Size: 2522 1465 2732
Fragments arranged by size:
2732 2522 1465
XbaI T'CTAG_A
Cuts at: 3750 3750
Size: 6719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 1134 2192 2761 3057 3195 4214 177
Size: 957 1058 569 296 138 1019 2682
Fragments arranged by size:
2682 1058 1019 957 569 296 138
XhoI C'TCGA_G
Cuts at: 1049 1049
Size: 6719
XmaI C'CCGG_G
Cuts at: 638 2504 3739 638
Size: 1866 1235 3618
Fragments arranged by size:
3618 1866 1235
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 6719
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AloI AscI BaeI BbvCI BcgI BfrBI
BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
NsiI PacI PfoI PmlI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI