Restriction map for
pF1KA0906
(Circular) (Six-base) MAPSORT of: pF1KA0906.seq Check: 7125 from: 1 to: 8774
pF1KA0906 8774 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2421 3579 5136 2421
Size: 1158 1557 6059
Fragments arranged by size:
6059 1557 1158
AatII G_ACGT'C
Cuts at: 860 3764 860
Size: 2904 5870
AccI GT'mk_AC
Cuts at: 661 785 860 2378 5812 661
Size: 124 75 1518 3434 3623
Fragments arranged by size:
3623 3434 1518 124 75
Acc65I G'GTAC_C
Cuts at: 5790 5790
Size: 8774
AclI AA'CG_TT
Cuts at: 8614 8614
Size: 8774
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 538 946 1545 2496 3184 5151 6599 7031
Size: 1 408 599 951 688 1967 1448 432
Cuts at: 7031 7384 8046 537
Size: 353 662 1265
Fragments arranged by size:
1967 1448 1265 951 688 662 599 432
408 353 1
AfeI AGC'GCT
Cuts at: 3477 3477
Size: 8774
AflIII A'CryG_T
Cuts at: 1025 1749 2399 3029 6126 7926 8207 1025
Size: 724 650 630 3097 1800 281 1592
Fragments arranged by size:
3097 1800 1592 724 650 630 281
AleI CACnn'nnGTG
Cuts at: 1169 1387 2000 1169
Size: 218 613 7943
Fragments arranged by size:
7943 613 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2479 3676 3736 2479
Size: 1197 60 7517
Fragments arranged by size:
7517 1197 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3688 3720 3688
Size: 32 8742
AlwNI CAG_nnn'CTG
Cuts at: 426 531 1600 1764 1912 3048 6079 7517 426
Size: 105 1069 164 148 1136 3031 1438 1683
Fragments arranged by size:
3031 1683 1438 1136 1069 164 148 105
ApaI G_GGCC'C
Cuts at: 169 982 1542 4423 5483 169
Size: 813 560 2881 1060 3460
Fragments arranged by size:
3460 2881 1060 813 560
ApaLI G'TGCA_C
Cuts at: 2944 4881 7612 2944
Size: 1937 2731 4106
Fragments arranged by size:
4106 2731 1937
ApoI r'AATT_y
Cuts at: 1454 2137 5778 8127 8383 1454
Size: 683 3641 2349 256 1845
Fragments arranged by size:
3641 2349 1845 683 256
AseI AT'TA_AT
Cuts at: 20 4003 20
Size: 3983 4791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8774
AvaI C'yCGr_G
Cuts at: 975 2125 2958 4497 4688 5609 5642 5794 975
Size: 1150 833 1539 191 921 33 152 3955
Fragments arranged by size:
3955 1539 1150 921 833 191 152 33
AvrII C'CTAG_G
Cuts at: 1149 1149
Size: 8774
BamHI G'GATC_C
Cuts at: 2046 2120 2228 3620 4699 5799 2046
Size: 74 108 1392 1079 1100 5021
Fragments arranged by size:
5021 1392 1100 1079 108 74
BanI G'GyrC_C
Cuts at: 1425 1516 2748 3652 3782 4730 5160 5245 5790
Size: 91 1232 904 130 948 430 85 545
Cuts at: 5790 6204 6452 6487 1425
Size: 414 248 35 3712
Fragments arranged by size:
3712 1232 948 904 545 430 414 248
130 91 85 35
BanII G_rGCy'C
Cuts at: 169 491 754 982 1542 1712 1957 2080 2774
Size: 322 263 228 560 170 245 123 694
Cuts at: 2774 2803 2849 2965 3530 4181 4255 4423 4636
Size: 29 46 116 565 651 74 168 213
Cuts at: 4636 5483 5752 5788 6818 169
Size: 847 269 36 1030 2125
Fragments arranged by size:
2125 1030 847 694 651 565 560 322
269 263 245 228 213 170 168 123
116 74 46 36 29
BbeI G_GCGC'C
Cuts at: 1520 6208 6456 1520
Size: 4688 248 3838
Fragments arranged by size:
4688 3838 248
BbsI GAAGACnn'nnnn_
Cuts at: 567 748 1562 2991 3091 4344 5098 567
Size: 181 814 1429 100 1253 754 4243
Fragments arranged by size:
4243 1429 1253 814 754 181 100
BbvCI CC'TCA_GC
Cuts at: 2159 2710 3865 2159
Size: 551 1155 7068
Fragments arranged by size:
7068 1155 551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 378 412 1614 1648 4150 4184 378
Size: 34 1202 34 2502 34 4968
Fragments arranged by size:
4968 2502 1202 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1138 1487 4230 6672 7728 8774 1138
Size: 349 2743 2442 1056 1046 1138
Fragments arranged by size:
2743 2442 1138 1056 1046 349
BclI T'GATC_A
Cuts at: 5579 6294 5579
Size: 715 8059
BfrBI ATG'CAT
Cuts at: 5704 5704
Size: 8774
BglI GCCn_nnn'nGGC
Cuts at: 112 181 4288 4613 5191 5299 5560 112
Size: 69 4107 325 578 108 261 3326
Fragments arranged by size:
4107 3326 578 325 261 108 69
BglII A'GATC_T
Cuts at: 6289 8326 6289
Size: 2037 6737
BlpI GC'TnA_GC
Cuts at: 2570 5886 2570
Size: 3316 5458
Bme1580I G_kGCm'C
Cuts at: 169 982 1347 1542 2948 3655 4423 4733 4885
Size: 813 365 195 1406 707 768 310 152
Cuts at: 4885 5165 5250 5483 6399 6492 7616 169
Size: 280 85 233 916 93 1124 1327
Fragments arranged by size:
1406 1327 1124 916 813 768 707 365
310 280 233 195 152 93 85
BmgBI CAC'GTC
Cuts at: 1028 1992 2790 4578 1028
Size: 964 798 1788 5224
Fragments arranged by size:
5224 1788 964 798
BmrI ACTGGGnnnn_n'
Cuts at: 2434 4941 5440 5986 6067 6402 8546 2434
Size: 2507 499 546 81 335 2144 2662
Fragments arranged by size:
2662 2507 2144 546 499 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1323 5518 7141 1323
Size: 4195 1623 2956
Fragments arranged by size:
4195 2956 1623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 517 532 1207 1786 4364 4650 5106 5281
Size: 249 15 675 579 2578 286 456 175
Cuts at: 5281 5397 268
Size: 116 3645
Fragments arranged by size:
3645 2578 675 579 456 286 249 175
116 15
Bpu10I CC'TnA_GC
Cuts at: 2159 2710 2865 3607 3865 5080 2159
Size: 551 155 742 258 1215 5853
Fragments arranged by size:
5853 1215 742 551 258 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1845 2020 4964 5950 6829 7543 7841 745
Size: 1100 175 2944 986 879 714 298 1678
Fragments arranged by size:
2944 1678 1100 986 879 714 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 983 1183 3544 4918 5182 8478 983
Size: 200 2361 1374 264 3296 1279
Fragments arranged by size:
3296 2361 1374 1279 264 200
BsaAI yAC'GTr
Cuts at: 665 1131 1750 3030 6127 665
Size: 466 619 1280 3097 3312
Fragments arranged by size:
3312 3097 1280 619 466
BsaBI GATnn'nnATC
Cuts at: 6 3664 6288 8325 6
Size: 3658 2624 2037 455
Fragments arranged by size:
3658 2624 2037 455
BsaHI Gr'CG_yC
Cuts at: 857 1517 1928 3761 3793 5357 6205 6453 7155
Size: 660 411 1833 32 1564 848 248 702
Cuts at: 7155 8651 857
Size: 1496 980
Fragments arranged by size:
1833 1564 1496 980 848 702 660 411
248 32
BsaWI w'CCGG_w
Cuts at: 5963 6484 7162 7573 7720 7994 5963
Size: 521 678 411 147 274 6743
Fragments arranged by size:
6743 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 2080 2110 3688 3718 4990 5020 649
Size: 30 1401 30 1578 30 1272 30 4403
Fragments arranged by size:
4403 1578 1401 1272 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2043 2070 4626 2043
Size: 27 2556 6191
Fragments arranged by size:
6191 2556 27
BseYI C'CCAG_C
Cuts at: 312 368 1050 1480 2290 2390 3009 3419 3481
Size: 56 682 430 810 100 619 410 62
Cuts at: 3481 4726 5332 5563 5751 6199 6957 7622 8030
Size: 1245 606 231 188 448 758 665 408
Cuts at: 8030 312
Size: 1056
Fragments arranged by size:
1245 1056 810 758 682 665 619 606
448 430 410 408 231 188 100 62
56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 916 1025 1684 2608 3553 3607 4070 4246
Size: 543 109 659 924 945 54 463 176
Cuts at: 4246 4426 5586 373
Size: 180 1160 3561
Fragments arranged by size:
3561 1160 945 924 659 543 463 180
176 109 54
BsiEI CG_ry'CG
Cuts at: 104 289 1003 1197 3843 4961 5294 5354 6316
Size: 185 714 194 2646 1118 333 60 962
Cuts at: 6316 6362 7592 104
Size: 46 1230 1286
Fragments arranged by size:
2646 1286 1230 1118 962 714 333 194
185 60 46
BsiHKAI G_wGCw'C
Cuts at: 337 491 754 1307 1919 2774 2849 2948 3839
Size: 154 263 553 612 855 75 99 891
Cuts at: 3839 4496 4583 4636 4885 5788 6566 6756 7616
Size: 657 87 53 249 903 778 190 860
Cuts at: 7616 337
Size: 1495
Fragments arranged by size:
1495 903 891 860 855 778 657 612
553 263 249 190 154 99 87 75
53
BsmI GAATG_Cn'
Cuts at: 5302 5302
Size: 8774
BsmBI CGTCTCn'nnnn_
Cuts at: 477 4388 5364 477
Size: 3911 976 3887
Fragments arranged by size:
3911 3887 976
Bsp1286I G_dGCh'C
Cuts at: 169 337 491 754 982 1307 1347 1542 1712
Size: 168 154 263 228 325 40 195 170
Cuts at: 1712 1919 1957 2080 2774 2803 2849 2948 2965
Size: 207 38 123 694 29 46 99 17
Cuts at: 2965 3530 3655 3839 4181 4255 4423 4496 4583
Size: 565 125 184 342 74 168 73 87
Cuts at: 4583 4636 4733 4885 5165 5250 5483 5752 5788
Size: 53 97 152 280 85 233 269 36
Cuts at: 5788 6399 6492 6566 6756 6818 7616 169
Size: 611 93 74 190 62 798 1327
Fragments arranged by size:
1327 798 694 611 565 342 325 280
269 263 233 228 207 195 190 184
170 168 168 154 152 125 123 99
97 93 87 85 74 74 73 62
53 46 40 38 36 29 17
BspHI T'CATG_A
Cuts at: 2975 5576 7206 2975
Size: 2601 1630 4543
Fragments arranged by size:
4543 2601 1630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2036 2250 2421 3387 3579 5136 5824 6038 6340
Size: 214 171 966 192 1557 688 214 302
Cuts at: 6340 6721 2036
Size: 381 4089
Fragments arranged by size:
4089 1557 966 688 381 302 214 214
192 171
BsrBI CCG'CTC
Cuts at: 50 7066 7120 8604 8769 50
Size: 7016 54 1484 165 55
Fragments arranged by size:
7016 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 3459 3720 5435 5704 6686 2015
Size: 1444 261 1715 269 982 4103
Fragments arranged by size:
4103 1715 1444 982 269 261
BsrFI r'CCGG_y
Cuts at: 1002 1192 2498 3553 3676 4956 6772 6953 1002
Size: 190 1306 1055 123 1280 1816 181 2823
Fragments arranged by size:
2823 1816 1306 1280 1055 190 181 123
BsrGI T'GTAC_A
Cuts at: 3050 3050
Size: 8774
BssSI C'ACGA_G
Cuts at: 2650 3529 3583 3787 7045 7753 2650
Size: 879 54 204 3258 708 3671
Fragments arranged by size:
3671 3258 879 708 204 54
BstAPI GCAn_nnn'nTGC
Cuts at: 2631 2952 3544 6151 2631
Size: 321 592 2607 5254
Fragments arranged by size:
5254 2607 592 321
BstBI TT'CG_AA
Cuts at: 7135 7135
Size: 8774
BstEII G'GTnAC_C
Cuts at: 1109 1818 2067 2811 3399 1109
Size: 709 249 744 588 6484
Fragments arranged by size:
6484 744 709 588 249
BstXI CCAn_nnnn'nTGG
Cuts at: 1815 2586 4569 1815
Size: 771 1983 6020
Fragments arranged by size:
6020 1983 771
BstYI r'GATC_y
Cuts at: 1 521 2046 2120 2228 3620 3938 4699 5613
Size: 520 1525 74 108 1392 318 761 914
Cuts at: 5613 5799 6266 6289 6624 6870 7176 7188 7274
Size: 186 467 23 335 246 306 12 86
Cuts at: 7274 8326 1
Size: 1052 449
Fragments arranged by size:
1525 1392 1052 914 761 520 467 449
335 318 306 246 186 108 86 74
23 12
Bsu36I CC'TnA_GG
Cuts at: 1777 4237 4735 5736 1777
Size: 2460 498 1001 4815
Fragments arranged by size:
4815 2460 1001 498
BtgI C'CryG_G
Cuts at: 107 1498 1542 2327 2939 3246 4562 4694 4847
Size: 1391 44 785 612 307 1316 132 153
Cuts at: 4847 4994 5369 5475 107
Size: 147 375 106 3406
Fragments arranged by size:
3406 1391 1316 785 612 375 307 153
147 132 106 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1318 2250 6724 6905 7068 1318
Size: 932 4474 181 163 3024
Fragments arranged by size:
4474 3024 932 181 163
BtsI GCAGTG_nn'
Cuts at: 337 353 1037 1042 1557 1632 1917 2675 2681
Size: 16 684 5 515 75 285 758 6
Cuts at: 2681 3373 3545 4468 5012 6017 337
Size: 692 172 923 544 1005 3094
Fragments arranged by size:
3094 1005 923 758 692 684 544 515
285 172 75 16 6 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1950 1985 1950
Size: 35 8739
DraI TTT'AAA
Cuts at: 5773 5773
Size: 8774
DrdI GACnn_nn'nnGTC
Cuts at: 1852 6480 7824 1852
Size: 4628 1344 2802
Fragments arranged by size:
4628 2802 1344
EaeI y'GGCC_r
Cuts at: 182 286 296 1000 1074 1194 1364 1986 2555
Size: 104 10 704 74 120 170 622 569
Cuts at: 2555 2610 3254 3261 3840 3917 4289 4589 4640
Size: 55 644 7 579 77 372 300 51
Cuts at: 4640 4958 5022 5291 6359 6533 6924 6951 8296
Size: 318 64 269 1068 174 391 27 1345
Cuts at: 8296 182
Size: 660
Fragments arranged by size:
1345 1068 704 660 644 622 579 569
391 372 318 300 269 174 170 120
104 77 74 64 55 51 27 10
7
EagI C'GGCC_G
Cuts at: 286 1000 1194 3840 4958 5291 6359 286
Size: 714 194 2646 1118 333 1068 2701
Fragments arranged by size:
2701 2646 1118 1068 714 333 194
EarI CTCTTCn'nnn_
Cuts at: 2801 5185 5509 5554 6797 7007 2801
Size: 2384 324 45 1243 210 4568
Fragments arranged by size:
4568 2384 1243 324 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 162 622 6982 7724 7870 8584 162
Size: 460 6360 742 146 714 352
Fragments arranged by size:
6360 742 714 460 352 146
EcoICRI GAG'CTC
Cuts at: 489 752 2772 2847 4634 5786 489
Size: 263 2020 75 1787 1152 3477
Fragments arranged by size:
3477 2020 1787 1152 263 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 517 532 537 538 946 1207 1545 1786
Size: 249 15 5 1 408 261 338 241
Cuts at: 1786 2496 3184 4364 4650 5106 5151 5281 5397
Size: 710 688 1180 286 456 45 130 116
Cuts at: 5397 6599 7031 7384 8046 268
Size: 1202 432 353 662 996
Fragments arranged by size:
1202 1180 996 710 688 662 456 432
408 353 338 286 261 249 241 130
116 45 15 5 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2782 3586 4236 5735 2782
Size: 804 650 1499 5821
Fragments arranged by size:
5821 1499 804 650
EcoO109I rG'GnC_Cy
Cuts at: 166 423 528 979 1538 1761 2107 3495 3579
Size: 257 105 451 559 223 346 1388 84
Cuts at: 3579 4281 4419 4420 4739 5285 5386 5913 166
Size: 702 138 1 319 546 101 527 3027
Fragments arranged by size:
3027 1388 702 559 546 527 451 346
319 257 223 138 105 101 84 1
EcoRI G'AATT_C
Cuts at: 5778 5778
Size: 8774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3147 6793 3147
Size: 3646 5128
FspI TGC'GCA
Cuts at: 6555 6555
Size: 8774
HaeII r_GCGC'y
Cuts at: 1520 3479 6208 6456 7686 8457 1520
Size: 1959 2729 248 1230 771 1837
Fragments arranged by size:
2729 1959 1837 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 2038 2070 2105 2137 3293 3325 3880
Size: 32 1405 32 35 32 1156 32 555
Cuts at: 3880 3912 4846 4878 4960 4992 5263 5295 6616
Size: 32 934 32 82 32 271 32 1321
Cuts at: 6616 6646 6648 6678 601
Size: 30 2 30 2697
Fragments arranged by size:
2697 1405 1321 1156 934 555 271 82
35 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 237 3066 5813 237
Size: 2829 2747 3198
Fragments arranged by size:
3198 2829 2747
HpaI GTT'AAC
Cuts at: 237 237
Size: 8774
KasI G'GCGC_C
Cuts at: 1516 6204 6452 1516
Size: 4688 248 3838
Fragments arranged by size:
4688 3838 248
KpnI G_GTAC'C
Cuts at: 5794 5794
Size: 8774
MluI A'CGCG_T
Cuts at: 8207 8207
Size: 8774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3014 3455 3825 7533 7717 3014
Size: 441 370 3708 184 4071
Fragments arranged by size:
4071 3708 441 370 184
MscI TGG'CCA
Cuts at: 298 1076 2557 2612 3256 3919 4591 6535 298
Size: 778 1481 55 644 663 672 1944 2537
Fragments arranged by size:
2537 1944 1481 778 672 663 644 55
MslI CAynn'nnrTG
Cuts at: 727 1030 1169 1387 1497 1682 2000 2779 2974
Size: 303 139 218 110 185 318 779 195
Cuts at: 2974 3058 3640 3658 3832 4109 4162 4567 5446
Size: 84 582 18 174 277 53 405 879
Cuts at: 5446 6763 6890 727
Size: 1317 127 2611
Fragments arranged by size:
2611 1317 879 779 582 405 318 303
277 218 195 185 174 139 127 110
84 53 18
MspA1I CmG'CkG
Cuts at: 178 272 312 1050 1565 1925 2158 2394 2412
Size: 94 40 738 515 360 233 236 18
Cuts at: 2412 2586 3248 3906 3969 4937 5070 5371 5572
Size: 174 662 658 63 968 133 301 201
Cuts at: 5572 5885 6199 6559 7343 7588 178
Size: 313 314 360 784 245 1364
Fragments arranged by size:
1364 968 784 738 662 658 515 360
360 314 313 301 245 236 233 201
174 133 94 63 40 18
NaeI GCC'GGC
Cuts at: 4958 6955 4958
Size: 1997 6777
NarI GG'CG_CC
Cuts at: 1517 6205 6453 1517
Size: 4688 248 3838
Fragments arranged by size:
4688 3838 248
NcoI C'CATG_G
Cuts at: 107 2939 4562 107
Size: 2832 1623 4319
Fragments arranged by size:
4319 2832 1623
NgoMIV G'CCGG_C
Cuts at: 4956 6953 4956
Size: 1997 6777
NsiI A_TGCA'T
Cuts at: 5706 5706
Size: 8774
NspI r_CATG'y
Cuts at: 1095 2403 4108 5827 6327 7930 1095
Size: 1308 1705 1719 500 1603 1939
Fragments arranged by size:
1939 1719 1705 1603 1308 500
PasI CC'CwG_GG
Cuts at: 1891 1950 2073 1891
Size: 59 123 8592
Fragments arranged by size:
8592 123 59
PciI A'CATG_T
Cuts at: 2399 7926 2399
Size: 5527 3247
PflMI CCAn_nnn'nTGG
Cuts at: 531 1719 1764 2117 531
Size: 1188 45 353 7188
Fragments arranged by size:
7188 1188 353 45
PfoI T'CCnGG_A
Cuts at: 773 2206 3764 4073 4814 5516 773
Size: 1433 1558 309 741 702 4031
Fragments arranged by size:
4031 1558 1433 741 702 309
PmeI GTTT'AAAC
Cuts at: 5773 5773
Size: 8774
PmlI CAC'GTG
Cuts at: 1750 1750
Size: 8774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3688 3720 7220 7252 3688
Size: 32 3500 32 5210
Fragments arranged by size:
5210 3500 32 32
PpuMI rG'GwC_Cy
Cuts at: 423 2107 3579 4739 5285 423
Size: 1684 1472 1160 546 3912
Fragments arranged by size:
3912 1684 1472 1160 546
PshAI GACnn'nnGTC
Cuts at: 3807 5572 3807
Size: 1765 7009
PspOMI G'GGCC_C
Cuts at: 165 978 1538 4419 5479 165
Size: 813 560 2881 1060 3460
Fragments arranged by size:
3460 2881 1060 813 560
PspXI vC'TCGA_Gb
Cuts at: 2125 5642 2125
Size: 3517 5257
PstI C_TGCA'G
Cuts at: 807 1049 1924 2420 3968 4034 5631 5821 6506
Size: 242 875 496 1548 66 1597 190 685
Cuts at: 6506 807
Size: 3075
Fragments arranged by size:
3075 1597 1548 875 685 496 242 190
66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8774
PvuII CAG'CTG
Cuts at: 1050 1565 2394 2586 3906 3969 4937 6199 6559
Size: 515 829 192 1320 63 968 1262 360
Cuts at: 6559 1050
Size: 3265
Fragments arranged by size:
3265 1320 1262 968 829 515 360 192
63
RsrII CG'GwC_CG
Cuts at: 3857 6969 3857
Size: 3112 5662
SacI G_AGCT'C
Cuts at: 491 754 2774 2849 4636 5788 491
Size: 263 2020 75 1787 1152 3477
Fragments arranged by size:
3477 2020 1787 1152 263 75
SacII CC_GC'GG
Cuts at: 3249 5372 3249
Size: 2123 6651
SalI G'TCGA_C
Cuts at: 5811 5811
Size: 8774
SanDI GG'GwC_CC
Cuts at: 5285 5285
Size: 8774
SapI GCTCTTCn'nnn_
Cuts at: 5554 6797 7007 5554
Size: 1243 210 7321
Fragments arranged by size:
7321 1243 210
SbfI CC_TGCA'GG
Cuts at: 5821 5821
Size: 8774
ScaI AGT'ACT
Cuts at: 5029 8291 5029
Size: 3262 5512
SexAI A'CCwGG_T
Cuts at: 1583 5528 1583
Size: 3945 4829
SfcI C'TryA_G
Cuts at: 33 803 1045 1420 1920 1977 2416 3964 4030
Size: 770 242 375 500 57 439 1548 66
Cuts at: 4030 4531 4823 5627 5817 6502 7470 7661 33
Size: 501 292 804 190 685 968 191 1146
Fragments arranged by size:
1548 1146 968 804 770 685 501 500
439 375 292 242 191 190 66 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 4288 4288
Size: 8774
SfoI GGC'GCC
Cuts at: 1518 6206 6454 1518
Size: 4688 248 3838
Fragments arranged by size:
4688 3838 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8774
SmaI CCC'GGG
Cuts at: 977 2960 4499 4690 5611 5796 977
Size: 1983 1539 191 921 185 3955
Fragments arranged by size:
3955 1983 1539 921 191 185
SmlI C'TyrA_G
Cuts at: 760 1824 2035 2125 4979 5642 5929 6844 7558
Size: 1064 211 90 2854 663 287 915 714
Cuts at: 7558 7820 760
Size: 262 1714
Fragments arranged by size:
2854 1714 1064 915 714 663 287 262
211 90
SnaBI TAC'GTA
Cuts at: 665 665
Size: 8774
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8774
SphI G_CATG'C
Cuts at: 1095 5827 6327 1095
Size: 4732 500 3542
Fragments arranged by size:
4732 3542 500
SrfI GCCC'GGGC
Cuts at: 4690 4690
Size: 8774
SspI AAT'ATT
Cuts at: 4001 4001
Size: 8774
StuI AGG'CCT
Cuts at: 253 2248 3728 4616 4912 253
Size: 1995 1480 888 296 4115
Fragments arranged by size:
4115 1995 1480 888 296
StyI C'CwwG_G
Cuts at: 107 1071 1149 2063 2116 2249 2939 3014 4562
Size: 964 78 914 53 133 690 75 1548
Cuts at: 4562 5382 5908 6262 107
Size: 820 526 354 2619
Fragments arranged by size:
2619 1548 964 914 820 690 526 354
133 78 75 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1170 2242 3670 4282 4359 4906 4934 5315 5703
Size: 1072 1428 612 77 547 28 381 388
Cuts at: 5703 6357 6490 6949 7158 1170
Size: 654 133 459 209 2786
Fragments arranged by size:
2786 1428 1072 654 612 547 459 388
381 209 133 77 28
TatI w'GTAC_w
Cuts at: 1526 3050 3815 5027 8289 1526
Size: 1524 765 1212 3262 2011
Fragments arranged by size:
3262 2011 1524 1212 765
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1461 1493 4295 4327 8291 8323 1461
Size: 32 2802 32 3964 32 1912
Fragments arranged by size:
3964 2802 1912 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 144 6571 144
Size: 6427 2347
XbaI T'CTAG_A
Cuts at: 5805 5805
Size: 8774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1549 2391 2550 4339 4674 5145 6269 1549
Size: 842 159 1789 335 471 1124 4054
Fragments arranged by size:
4054 1789 1124 842 471 335 159
XhoI C'TCGA_G
Cuts at: 2125 5642 2125
Size: 3517 5257
XmaI C'CCGG_G
Cuts at: 975 2958 4497 4688 5609 5794 975
Size: 1983 1539 191 921 185 3955
Fragments arranged by size:
3955 1983 1539 921 191 185
XmnI GAAnn'nnTTC
Cuts at: 550 3130 3338 3700 4091 550
Size: 2580 208 362 391 5233
Fragments arranged by size:
5233 2580 391 362 208
ZraI GAC'GTC
Cuts at: 858 3762 858
Size: 2904 5870
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AscI BaeI BmtI BsiWI BspEI
BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI PacI PsiI PsrI
SgrAI SwaI