Restriction map for
pF1KA0904
(Circular) (Six-base) MAPSORT of: pF1KA0904.seq Check: 1902 from: 1 to: 7556
pF1KA0904 7556 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4034 4034
Size: 7556
AatII G_ACGT'C
Cuts at: 3255 3255
Size: 7556
AccI GT'mk_AC
Cuts at: 3051 4594 3051
Size: 1543 6013
Acc65I G'GTAC_C
Cuts at: 2807 4572 2807
Size: 1765 5791
AclI AA'CG_TT
Cuts at: 1984 2841 7396 1984
Size: 857 4555 2144
Fragments arranged by size:
4555 2144 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1517 2392 2786 3662 4025 4363 5381 5813
Size: 820 875 394 876 363 338 1018 432
Cuts at: 5813 6166 6828 697
Size: 353 662 1425
Fragments arranged by size:
1425 1018 876 875 820 662 432 394
363 353 338
AflIII A'CryG_T
Cuts at: 2092 4908 6708 6989 2092
Size: 2816 1800 281 2659
Fragments arranged by size:
2816 2659 1800 281
AleI CACnn'nnGTG
Cuts at: 2459 2459
Size: 7556
AlwNI CAG_nnn'CTG
Cuts at: 1333 1486 3555 4188 4861 6299 1333
Size: 153 2069 633 673 1438 2590
Fragments arranged by size:
2590 2069 1438 673 633 153
ApaI G_GGCC'C
Cuts at: 3830 4465 3830
Size: 635 6921
ApaLI G'TGCA_C
Cuts at: 2593 3132 6394 2593
Size: 539 3262 3755
Fragments arranged by size:
3755 3262 539
ApoI r'AATT_y
Cuts at: 1527 1905 2216 2667 3061 4560 6909 7165 1527
Size: 378 311 451 394 1499 2349 256 1918
Fragments arranged by size:
2349 1918 1499 451 394 378 311 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7556
AvaI C'yCGr_G
Cuts at: 464 819 873 915 4576 464
Size: 355 54 42 3661 3444
Fragments arranged by size:
3661 3444 355 54 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1928 1961 1928
Size: 33 7523
BamHI G'GATC_C
Cuts at: 4581 4581
Size: 7556
BanI G'GyrC_C
Cuts at: 2527 2807 4572 4986 5234 5269 2527
Size: 280 1765 414 248 35 4814
Fragments arranged by size:
4814 1765 414 280 248 35
BanII G_rGCy'C
Cuts at: 775 988 2132 3830 3887 4465 4570 5600 775
Size: 213 1144 1698 57 578 105 1030 2731
Fragments arranged by size:
2731 1698 1144 1030 578 213 105 57
BbeI G_GCGC'C
Cuts at: 4990 5238 4990
Size: 248 7308
BbsI GAAGACnn'nnnn_
Cuts at: 661 1942 2250 4466 661
Size: 1281 308 2216 3751
Fragments arranged by size:
3751 2216 1281 308
BbvCI CC'TCA_GC
Cuts at: 2189 3368 4349 2189
Size: 1179 981 5396
Fragments arranged by size:
5396 1179 981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 421 455 90
Size: 34 297 34 7191
Fragments arranged by size:
7191 297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5454 6510 7556 5454
Size: 1056 1046 5454
Fragments arranged by size:
5454 1056 1046
BclI T'GATC_A
Cuts at: 2948 5076 2948
Size: 2128 5428
BglI GCCn_nnn'nGGC
Cuts at: 3825 3825
Size: 7556
BglII A'GATC_T
Cuts at: 1006 2142 5071 7108 1006
Size: 1136 2929 2037 1454
Fragments arranged by size:
2929 2037 1454 1136
BlpI GC'TnA_GC
Cuts at: 2936 4668 2936
Size: 1732 5824
Bme1580I G_kGCm'C
Cuts at: 301 689 2597 3136 3585 3830 4255 4465 5181
Size: 388 1908 539 449 245 425 210 716
Cuts at: 5181 5274 6398 301
Size: 93 1124 1459
Fragments arranged by size:
1908 1459 1124 716 539 449 425 388
245 210 93
BmgBI CAC'GTC
Cuts at: 2095 2095
Size: 7556
BmrI ACTGGGnnnn_n'
Cuts at: 1073 1145 2271 3658 4433 4464 4768 4849 5184
Size: 72 1126 1387 775 31 304 81 335
Cuts at: 5184 7328 1073
Size: 2144 1301
Fragments arranged by size:
2144 1387 1301 1126 775 335 304 81
72 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1318 3518 5923 1318
Size: 2200 2405 2951
Fragments arranged by size:
2951 2405 2200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 2039 2108 2147 2801 3182 3808 501
Size: 1538 69 39 654 381 626 4249
Fragments arranged by size:
4249 1538 654 626 381 69 39
Bpu10I CC'TnA_GC
Cuts at: 1117 1418 2189 3368 3493 3881 4308 4349 4437
Size: 301 771 1179 125 388 427 41 88
Cuts at: 4437 1117
Size: 4236
Fragments arranged by size:
4236 1179 771 427 388 301 125 88
41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1925 3768 4068 4732 5611 6325 6623 1925
Size: 1843 300 664 879 714 298 2858
Fragments arranged by size:
2858 1843 879 714 664 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 1133 1672 2202 3613 4044 4375 7260 1133
Size: 539 530 1411 431 331 2885 1429
Fragments arranged by size:
2885 1429 1411 539 530 431 331
BsaAI yAC'GTr
Cuts at: 4909 4909
Size: 7556
BsaBI GATnn'nnATC
Cuts at: 6 5070 7107 6
Size: 5064 2037 455
Fragments arranged by size:
5064 2037 455
BsaHI Gr'CG_yC
Cuts at: 3048 3252 4987 5235 5937 7433 3048
Size: 204 1735 248 702 1496 3171
Fragments arranged by size:
3171 1735 1496 702 248 204
BsaWI w'CCGG_w
Cuts at: 542 695 4745 5266 5944 6355 6502 6776 542
Size: 153 4050 521 678 411 147 274 1322
Fragments arranged by size:
4050 1322 678 521 411 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 664 690 694 720 1722 1752 1974
Size: 30 294 26 4 26 1002 30 222
Cuts at: 1974 2004 2348 2378 3514 3544 340
Size: 30 344 30 1136 30 4352
Fragments arranged by size:
4352 1136 1002 344 294 222 30 30
30 30 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 167 589 1651 3185 3649 3871 4553 167
Size: 422 1062 1534 464 222 682 3170
Fragments arranged by size:
3170 1534 1062 682 464 422 222
BseYI C'CCAG_C
Cuts at: 3357 3400 3602 3963 3998 4407 4981 5739 6404
Size: 43 202 361 35 409 574 758 665
Cuts at: 6404 6812 3357
Size: 408 4101
Fragments arranged by size:
4101 758 665 574 409 408 361 202
43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 4285 415
Size: 3870 3686
BsiEI CG_ry'CG
Cuts at: 104 426 1036 5098 5144 6374 104
Size: 322 610 4062 46 1230 1286
Fragments arranged by size:
4062 1286 1230 610 322 46
BsiHKAI G_wGCw'C
Cuts at: 2132 2597 3136 4040 4570 5348 5538 6398 2132
Size: 465 539 904 530 778 190 860 3290
Fragments arranged by size:
3290 904 860 778 539 530 465 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1023 1023
Size: 7556
Bsp1286I G_dGCh'C
Cuts at: 301 689 775 988 2132 2597 3136 3585 3830
Size: 388 86 213 1144 465 539 449 245
Cuts at: 3830 3887 4040 4255 4465 4570 5181 5274 5348
Size: 57 153 215 210 105 611 93 74
Cuts at: 5348 5538 5600 6398 301
Size: 190 62 798 1459
Fragments arranged by size:
1459 1144 798 611 539 465 449 388
245 215 213 210 190 153 105 93
86 74 62 57
BspHI T'CATG_A
Cuts at: 2624 5988 2624
Size: 3364 4192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 3479 4034 4606 4820 5122 5503 591
Size: 2888 555 572 214 302 381 2644
Fragments arranged by size:
2888 2644 572 555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5848 5902 7386 7551 50
Size: 5798 54 1484 165 55
Fragments arranged by size:
5798 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5468 5468
Size: 7556
BsrFI r'CCGG_y
Cuts at: 5554 5735 5554
Size: 181 7375
BsrGI T'GTAC_A
Cuts at: 1920 1920
Size: 7556
BssSI C'ACGA_G
Cuts at: 5827 6535 5827
Size: 708 6848
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 3658 4933 1333
Size: 2325 1275 3956
Fragments arranged by size:
3956 2325 1275
BstBI TT'CG_AA
Cuts at: 568 5917 568
Size: 5349 2207
BstEII G'GTnAC_C
Cuts at: 273 977 273
Size: 704 6852
BstXI CCAn_nnnn'nTGG
Cuts at: 264 2984 264
Size: 2720 4836
BstYI r'GATC_y
Cuts at: 1 763 1006 1228 2142 3117 4581 5048 5071
Size: 762 243 222 914 975 1464 467 23
Cuts at: 5071 5406 5652 5958 5970 6056 7108 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1464 1052 975 914 762 467 449 335
306 246 243 222 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3321 3321
Size: 7556
BtgI C'CryG_G
Cuts at: 3623 3623
Size: 7556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5506 5687 5850 5506
Size: 181 163 7212
Fragments arranged by size:
7212 181 163
BtsI GCAGTG_nn'
Cuts at: 3464 4799 3464
Size: 1335 6221
ClaI AT'CG_AT
Cuts at: 532 532
Size: 7556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3795 3830 3795
Size: 35 7521
DraI TTT'AAA
Cuts at: 4555 4555
Size: 7556
DraIII CAC_nnn'GTG
Cuts at: 4323 4323
Size: 7556
DrdI GACnn_nn'nnGTC
Cuts at: 5262 6606 5262
Size: 1344 6212
EaeI y'GGCC_r
Cuts at: 834 2322 4020 5141 5315 5706 5733 7078 834
Size: 1488 1698 1121 174 391 27 1345 1312
Fragments arranged by size:
1698 1488 1345 1312 1121 391 174 27
EagI C'GGCC_G
Cuts at: 5141 5141
Size: 7556
EarI CTCTTCn'nnn_
Cuts at: 1219 1697 1790 2057 2762 3275 3865 4524 5579
Size: 478 93 267 705 513 590 659 1055
Cuts at: 5579 5789 1219
Size: 210 2986
Fragments arranged by size:
2986 1055 705 659 590 513 478 267
210 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5764 6506 6652 7366 5764
Size: 742 146 714 5954
Fragments arranged by size:
5954 742 714 146
EcoICRI GAG'CTC
Cuts at: 2130 4568 2130
Size: 2438 5118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 697 1517 2039 2108 2147 2392 2786 2801
Size: 196 820 522 69 39 245 394 15
Cuts at: 2801 3182 3662 3808 4025 4363 5381 5813 6166
Size: 381 480 146 217 338 1018 432 353
Cuts at: 6166 6828 501
Size: 662 1229
Fragments arranged by size:
1229 1018 820 662 522 480 432 394
381 353 338 245 217 196 146 69
39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 918 993 1023 4115 918
Size: 75 30 3092 4359
Fragments arranged by size:
4359 3092 75 30
EcoO109I rG'GnC_Cy
Cuts at: 3826 3827 3875 4202 4284 4302 4461 4501 4695
Size: 1 48 327 82 18 159 40 194
Cuts at: 4695 3826
Size: 6687
Fragments arranged by size:
6687 327 194 159 82 48 40 18
1
EcoRI G'AATT_C
Cuts at: 3061 4560 3061
Size: 1499 6057
EcoRV GAT'ATC
Cuts at: 333 548 333
Size: 215 7341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1076 2068 3952 5575 1076
Size: 992 1884 1623 3057
Fragments arranged by size:
3057 1884 1623 992
FspI TGC'GCA
Cuts at: 5337 5337
Size: 7556
HaeII r_GCGC'y
Cuts at: 3947 4990 5238 6468 7239 3947
Size: 1043 248 1230 771 4264
Fragments arranged by size:
4264 1230 1043 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 340 354 372 532 564 664 690 696
Size: 18 14 18 160 32 100 26 6
Cuts at: 696 722 802 834 1125 1157 1549 1581 1632
Size: 26 80 32 291 32 392 32 51
Cuts at: 1632 1664 2079 2111 3102 3134 5398 5428 5430
Size: 32 415 32 991 32 2264 30 2
Cuts at: 5430 5460 322
Size: 30 2418
Fragments arranged by size:
2418 2264 991 415 392 291 160 100
80 51 32 32 32 32 32 32
32 30 30 26 26 18 18 14
6 2
HincII GTy'rAC
Cuts at: 2469 4595 2469
Size: 2126 5430
HindIII A'AGCT_T
Cuts at: 3701 3701
Size: 7556
HpaI GTT'AAC
Cuts at: 2469 2469
Size: 7556
KasI G'GCGC_C
Cuts at: 4986 5234 4986
Size: 248 7308
KpnI G_GTAC'C
Cuts at: 2811 4576 2811
Size: 1765 5791
MfeI C'AATT_G
Cuts at: 1700 1700
Size: 7556
MluI A'CGCG_T
Cuts at: 6989 6989
Size: 7556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 1117 1535 3557 6315 6499 371
Size: 746 418 2022 2758 184 1428
Fragments arranged by size:
2758 2022 1428 746 418 184
MscI TGG'CCA
Cuts at: 836 2324 4022 5317 836
Size: 1488 1698 1295 3075
Fragments arranged by size:
3075 1698 1488 1295
MslI CAynn'nnrTG
Cuts at: 2459 5545 5672 2459
Size: 3086 127 4343
Fragments arranged by size:
4343 3086 127
MspA1I CmG'CkG
Cuts at: 1067 1330 1957 2980 3139 3438 3516 3555 3718
Size: 263 627 1023 159 299 78 39 163
Cuts at: 3718 3756 4667 4981 5341 6125 6370 1067
Size: 38 911 314 360 784 245 2253
Fragments arranged by size:
2253 1023 911 784 627 360 314 299
263 245 163 159 78 39 38
NaeI GCC'GGC
Cuts at: 5737 5737
Size: 7556
NarI GG'CG_CC
Cuts at: 4987 5235 4987
Size: 248 7308
NcoI C'CATG_G
Cuts at: 3623 3623
Size: 7556
NgoMIV G'CCGG_C
Cuts at: 5735 5735
Size: 7556
NspI r_CATG'y
Cuts at: 3108 3726 4378 4609 5109 6712 3108
Size: 618 652 231 500 1603 3952
Fragments arranged by size:
3952 1603 652 618 500 231
PasI CC'CwG_GG
Cuts at: 294 294
Size: 7556
PciI A'CATG_T
Cuts at: 6708 6708
Size: 7556
PflMI CCAn_nnn'nTGG
Cuts at: 4188 4188
Size: 7556
PfoI T'CCnGG_A
Cuts at: 766 903 766
Size: 137 7419
PmeI GTTT'AAAC
Cuts at: 4555 4555
Size: 7556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1218 1250 6002 6034 1218
Size: 32 4752 32 2740
Fragments arranged by size:
4752 2740 32 32
PpuMI rG'GwC_Cy
Cuts at: 4202 4284 4302 4202
Size: 82 18 7456
Fragments arranged by size:
7456 82 18
PshAI GACnn'nnGTC
Cuts at: 401 851 1035 401
Size: 450 184 6922
Fragments arranged by size:
6922 450 184
PspOMI G'GGCC_C
Cuts at: 3826 4461 3826
Size: 635 6921
PspXI vC'TCGA_Gb
Cuts at: 873 873
Size: 7556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 7524
PstI C_TGCA'G
Cuts at: 1344 3084 3479 4603 5288 1344
Size: 1740 395 1124 685 3612
Fragments arranged by size:
3612 1740 1124 685 395
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7556
PvuII CAG'CTG
Cuts at: 1330 1957 2980 3139 3438 3516 3555 3718 3756
Size: 627 1023 159 299 78 39 163 38
Cuts at: 3756 4981 5341 1330
Size: 1225 360 3545
Fragments arranged by size:
3545 1225 1023 627 360 299 163 159
78 39 38
RsrII CG'GwC_CG
Cuts at: 5751 5751
Size: 7556
SacI G_AGCT'C
Cuts at: 2132 4570 2132
Size: 2438 5118
SalI G'TCGA_C
Cuts at: 4593 4593
Size: 7556
SanDI GG'GwC_CC
Cuts at: 4302 4302
Size: 7556
SapI GCTCTTCn'nnn_
Cuts at: 1790 3275 5579 5789 1790
Size: 1485 2304 210 3557
Fragments arranged by size:
3557 2304 1485 210
SbfI CC_TGCA'GG
Cuts at: 4603 4603
Size: 7556
ScaI AGT'ACT
Cuts at: 1848 3333 4063 7073 1848
Size: 1485 730 3010 2331
Fragments arranged by size:
3010 2331 1485 730
SfcI C'TryA_G
Cuts at: 33 990 1020 1340 3080 3151 3475 4599 5284
Size: 957 30 320 1740 71 324 1124 685
Cuts at: 5284 6252 6443 33
Size: 968 191 1146
Fragments arranged by size:
1740 1146 1124 968 957 685 324 320
191 71 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3825 3825
Size: 7556
SfoI GGC'GCC
Cuts at: 4988 5236 4988
Size: 248 7308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7556
SmaI CCC'GGG
Cuts at: 466 4578 466
Size: 4112 3444
SmlI C'TyrA_G
Cuts at: 873 915 1940 3747 4047 4711 5626 6340 6602
Size: 42 1025 1807 300 664 915 714 262
Cuts at: 6602 873
Size: 1827
Fragments arranged by size:
1827 1807 1025 915 714 664 300 262
42
SpeI A'CTAG_T
Cuts at: 67 2361 67
Size: 2294 5262
SphI G_CATG'C
Cuts at: 4609 5109 4609
Size: 500 7056
StuI AGG'CCT
Cuts at: 3416 4045 4448 3416
Size: 629 403 6524
Fragments arranged by size:
6524 629 403
StyI C'CwwG_G
Cuts at: 1367 2339 3623 4175 4227 4690 5044 1367
Size: 972 1284 552 52 463 354 3879
Fragments arranged by size:
3879 1284 972 552 463 354 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 398 505 741 918 1092 5139 5272 5731 5940
Size: 107 236 177 174 4047 133 459 209
Cuts at: 5940 398
Size: 2014
Fragments arranged by size:
4047 2014 459 236 209 177 174 133
107
TatI w'GTAC_w
Cuts at: 1846 1920 3331 4061 7071 1846
Size: 74 1411 730 3010 2331
Fragments arranged by size:
3010 2331 1411 730 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 2097 2129 2259 2291 2348 2380 2674
Size: 32 1620 32 130 32 57 32 294
Cuts at: 2674 2706 7073 7105 445
Size: 32 4367 32 896
Fragments arranged by size:
4367 1620 896 294 130 57 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4076 5353 4076
Size: 1277 6279
XbaI T'CTAG_A
Cuts at: 4587 4587
Size: 7556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2928 5051 2928
Size: 2123 5433
XhoI C'TCGA_G
Cuts at: 873 915 873
Size: 42 7514
XmaI C'CCGG_G
Cuts at: 464 4576 464
Size: 4112 3444
XmnI GAAnn'nnTTC
Cuts at: 2051 2051
Size: 7556
ZraI GAC'GTC
Cuts at: 3253 3253
Size: 7556
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI AscI AvrII
BfrBI BmtI BsiWI BsmI BspEI BssHII BstZ17I FseI
FspAI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI SacII SexAI SgrAI SnaBI SrfI SspI SwaI