Restriction map for
pF1KA0900
(Circular) (Six-base) MAPSORT of: pF1KA0900.seq Check: 4374 from: 1 to: 4619
pF1KA0900 4619 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1657 1657
Size: 4619
Acc65I G'GTAC_C
Cuts at: 1635 1635
Size: 4619
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 272 571 2444 2876 3623 3891 185
Size: 87 299 1873 432 747 268 913
Fragments arranged by size:
1873 913 747 432 299 268 87
AflIII A'CryG_T
Cuts at: 1971 3075 4052 1971
Size: 1104 977 2538
Fragments arranged by size:
2538 1104 977
AgeI A'CCGG_T
Cuts at: 3007 3007
Size: 4619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1418 1418
Size: 4619
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1591 1623 1591
Size: 32 4587
AlwNI CAG_nnn'CTG
Cuts at: 1381 1924 3491 1381
Size: 543 1567 2509
Fragments arranged by size:
2509 1567 543
ApaI G_GGCC'C
Cuts at: 1306 1306
Size: 4619
ApaLI G'TGCA_C
Cuts at: 3389 3389
Size: 4619
ApoI r'AATT_y
Cuts at: 306 1460 1623 3972 4228 306
Size: 1154 163 2349 256 697
Fragments arranged by size:
2349 1154 697 256 163
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 1299 1336 1639 1299
Size: 37 303 4279
Fragments arranged by size:
4279 303 37
BamHI G'GATC_C
Cuts at: 802 1644 802
Size: 842 3777
BanI G'GyrC_C
Cuts at: 667 710 1635 2049 2297 2332 667
Size: 43 925 414 248 35 2954
Fragments arranged by size:
2954 925 414 248 43 35
BanII G_rGCy'C
Cuts at: 137 159 1277 1306 1633 2663 137
Size: 22 1118 29 327 1030 2093
Fragments arranged by size:
2093 1118 1030 327 29 22
BbeI G_GCGC'C
Cuts at: 714 2053 2301 714
Size: 1339 248 3032
Fragments arranged by size:
3032 1339 248
BbsI GAAGACnn'nnnn_
Cuts at: 260 623 785 1334 260
Size: 363 162 549 3545
Fragments arranged by size:
3545 549 363 162
BbvCI CC'TCA_GC
Cuts at: 1010 1167 1382 1010
Size: 157 215 4247
Fragments arranged by size:
4247 215 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4585
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3278 4619 2517
Size: 761 1341 2517
Fragments arranged by size:
2517 1341 761
BclI T'GATC_A
Cuts at: 2139 2139
Size: 4619
BfrBI ATG'CAT
Cuts at: 832 1365 832
Size: 533 4086
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 229 1731 229
Size: 1502 3117
Bme1580I G_kGCm'C
Cuts at: 1306 2244 2337 3393 1306
Size: 938 93 1056 2532
Fragments arranged by size:
2532 1056 938 93
BmgBI CAC'GTC
Cuts at: 921 1527 921
Size: 606 4013
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1831 1912 2247 4391 1369
Size: 462 81 335 2144 1597
Fragments arranged by size:
2144 1597 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1180 1061
Size: 119 4500
Bpu10I CC'TnA_GC
Cuts at: 1010 1167 1382 1010
Size: 157 215 4247
Fragments arranged by size:
4247 215 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 2674 3166 3464 1795
Size: 879 492 298 2950
Fragments arranged by size:
2950 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 381 4323 381
Size: 3942 677
BsaAI yAC'GTr
Cuts at: 1972 1972
Size: 4619
BsaBI GATnn'nnATC
Cuts at: 6 2133 4170 6
Size: 2127 2037 455
Fragments arranged by size:
2127 2037 455
BsaHI Gr'CG_yC
Cuts at: 639 711 2050 2298 3000 4496 639
Size: 72 1339 248 702 1496 762
Fragments arranged by size:
1496 1339 762 702 248 72
BsaWI w'CCGG_w
Cuts at: 1129 1808 2329 3007 3281 3428 3839 1129
Size: 679 521 678 274 147 411 1909
Fragments arranged by size:
1909 679 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 946 976 1249 1279 946
Size: 30 273 30 4286
Fragments arranged by size:
4286 273 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1130 1142 1241 1244 1429 1130
Size: 12 99 3 185 4320
Fragments arranged by size:
4320 185 99 12 3
BseYI C'CCAG_C
Cuts at: 350 887 1098 2044 2802 3379 3875 350
Size: 537 211 946 758 577 496 1094
Fragments arranged by size:
1094 946 758 577 537 496 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1049 1388 1498 865
Size: 184 339 110 3986
Fragments arranged by size:
3986 339 184 110
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3415 104
Size: 2057 46 1208 1308
Fragments arranged by size:
2057 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 159 1633 2411 2601 3393 159
Size: 1474 778 190 792 1385
Fragments arranged by size:
1474 1385 792 778 190
BsmI GAATG_Cn'
Cuts at: 455 455
Size: 4619
Bsp1286I G_dGCh'C
Cuts at: 137 159 1277 1306 1633 2244 2337 2411 2601
Size: 22 1118 29 327 611 93 74 190
Cuts at: 2601 2663 3393 137
Size: 62 730 1363
Fragments arranged by size:
1363 1118 730 611 327 190 93 74
62 29 22
BspEI T'CCGG_A
Cuts at: 1129 3839 1129
Size: 2710 1909
BspHI T'CATG_A
Cuts at: 3795 3795
Size: 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1669 1883 2185 2566 1669
Size: 214 302 381 3722
Fragments arranged by size:
3722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 2531 1550
Size: 981 3638
BsrFI r'CCGG_y
Cuts at: 965 2617 2798 3007 965
Size: 1652 181 209 2577
Fragments arranged by size:
2577 1652 209 181
BssSI C'ACGA_G
Cuts at: 2890 3248 2890
Size: 358 4261
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1996 1177
Size: 819 3800
BstBI TT'CG_AA
Cuts at: 1452 2980 1452
Size: 1528 3091
BstYI r'GATC_y
Cuts at: 1 802 1644 2111 2134 2469 2715 3727 3813
Size: 801 842 467 23 335 246 1012 86
Cuts at: 3813 3825 4171 1
Size: 12 346 449
Fragments arranged by size:
1012 842 801 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1037 1037
Size: 4619
BtgI C'CryG_G
Cuts at: 783 1556 783
Size: 773 3846
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 1862 1862
Size: 4619
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3183 2325
Size: 858 3761
EaeI y'GGCC_r
Cuts at: 1529 2204 2378 2769 2796 4141 1529
Size: 675 174 391 27 1345 2007
Fragments arranged by size:
2007 1345 675 391 174 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 115 317 365 2642 2852 115
Size: 202 48 2277 210 1882
Fragments arranged by size:
2277 1882 210 202 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1133 2827 3137 3283 4429 1133
Size: 1694 310 146 1146 1323
Fragments arranged by size:
1694 1323 1146 310 146
EcoICRI GAG'CTC
Cuts at: 157 1631 157
Size: 1474 3145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 272 571 1061 1180 2444 2876 3623 3891
Size: 87 299 490 119 1264 432 747 268
Cuts at: 3891 185
Size: 913
Fragments arranged by size:
1264 913 747 490 432 299 268 119
87
EcoNI CCTnn'n_nnAGG
Cuts at: 984 984
Size: 4619
EcoO109I rG'GnC_Cy
Cuts at: 1041 1052 1324 1758 1041
Size: 11 272 434 3902
Fragments arranged by size:
3902 434 272 11
EcoRI G'AATT_C
Cuts at: 1460 1623 1460
Size: 163 4456
EcoRV GAT'ATC
Cuts at: 390 390
Size: 4619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4619
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 714 2053 2301 3323 4302 714
Size: 1339 248 1022 979 1031
Fragments arranged by size:
1339 1031 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 808 840 1567 1599 2461 2491 2493 2523 808
Size: 32 727 32 862 30 2 30 2904
Fragments arranged by size:
2904 862 727 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1658 1658
Size: 4619
HindIII A'AGCT_T
Cuts at: 404 404
Size: 4619
KasI G'GCGC_C
Cuts at: 710 2049 2297 710
Size: 1339 248 3032
Fragments arranged by size:
3032 1339 248
KpnI G_GTAC'C
Cuts at: 1639 1639
Size: 4619
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1634 3290 3474 1634
Size: 1656 184 2779
Fragments arranged by size:
2779 1656 184
MscI TGG'CCA
Cuts at: 1531 2380 1531
Size: 849 3770
MslI CAynn'nnrTG
Cuts at: 1555 2608 2735 1555
Size: 1053 127 3439
Fragments arranged by size:
3439 1053 127
MspA1I CmG'CkG
Cuts at: 228 354 913 1488 1730 2044 2404 3417 3662
Size: 126 559 575 242 314 360 1013 245
Cuts at: 3662 228
Size: 1185
Fragments arranged by size:
1185 1013 575 559 360 314 245 242
126
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 711 2050 2298 711
Size: 1339 248 3032
Fragments arranged by size:
3032 1339 248
NcoI C'CATG_G
Cuts at: 783 783
Size: 4619
NdeI CA'TA_TG
Cuts at: 735 735
Size: 4619
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NsiI A_TGCA'T
Cuts at: 834 1367 834
Size: 533 4086
NspI r_CATG'y
Cuts at: 250 832 1672 2172 3079 250
Size: 582 840 500 907 1790
Fragments arranged by size:
1790 907 840 582 500
PciI A'CATG_T
Cuts at: 3075 3075
Size: 4619
PflMI CCAn_nnn'nTGG
Cuts at: 438 1366 438
Size: 928 3691
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4587
PpuMI rG'GwC_Cy
Cuts at: 1052 1052
Size: 4619
PspOMI G'GGCC_C
Cuts at: 1302 1302
Size: 4619
PstI C_TGCA'G
Cuts at: 1666 2351 1666
Size: 685 3934
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 228 354 913 2044 2404 228
Size: 126 559 1131 360 2443
Fragments arranged by size:
2443 1131 559 360 126
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 159 1633 159
Size: 1474 3145
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SanDI GG'GwC_CC
Cuts at: 1052 1052
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 2642 2852 2642
Size: 210 4409
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 204 4136 204
Size: 3932 687
SfcI C'TryA_G
Cuts at: 33 382 604 1662 2347 3340 3531 33
Size: 349 222 1058 685 993 191 1121
Fragments arranged by size:
1121 1058 993 685 349 222 191
SfoI GGC'GCC
Cuts at: 712 2051 2299 712
Size: 1339 248 3032
Fragments arranged by size:
3032 1339 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1301 1338 1641 1301
Size: 37 303 4279
Fragments arranged by size:
4279 303 37
SmlI C'TyrA_G
Cuts at: 1774 2689 3181 3443 1774
Size: 915 492 262 2950
Fragments arranged by size:
2950 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
StuI AGG'CCT
Cuts at: 1060 1060
Size: 4619
StyI C'CwwG_G
Cuts at: 783 1753 2107 783
Size: 970 354 3295
Fragments arranged by size:
3295 970 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 1274 2202 2335 2794 3003 364
Size: 910 928 133 459 209 1980
Fragments arranged by size:
1980 928 910 459 209 133
TatI w'GTAC_w
Cuts at: 202 1562 4134 202
Size: 1360 2572 687
Fragments arranged by size:
2572 1360 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1249 1281 4136 4168 1249
Size: 32 2855 32 1700
Fragments arranged by size:
2855 1700 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1275 2114 1275
Size: 839 3780
XmaI C'CCGG_G
Cuts at: 1299 1336 1639 1299
Size: 37 303 4279
Fragments arranged by size:
4279 303 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI