Restriction map for
pF1KA0892
(Circular) (Six-base) MAPSORT of: pF1KA0892.seq Check: 445 from: 1 to: 4952
pF1KA0892 4952 bp
With 182 enzymes: SgfI *
June 11, 2014 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 320 831 320
Size: 511 4441
AccI GT'mk_AC
Cuts at: 983 1990 983
Size: 1007 3945
Acc65I G'GTAC_C
Cuts at: 182 1968 182
Size: 1786 3166
AclI AA'CG_TT
Cuts at: 4792 4792
Size: 4952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1585 2777 3209 3956 4224 886
Size: 699 1192 432 747 268 1614
Fragments arranged by size:
1614 1192 747 699 432 268
AflIII A'CryG_T
Cuts at: 968 1709 2304 3408 4385 968
Size: 741 595 1104 977 1535
Fragments arranged by size:
1535 1104 977 741 595
AgeI A'CCGG_T
Cuts at: 3340 3340
Size: 4952
AhdI GACnn_n'nnGTC
Cuts at: 591 1883 591
Size: 1292 3660
AleI CACnn'nnGTG
Cuts at: 971 971
Size: 4952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 4952
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1294 1326 1294
Size: 32 4920
AlwNI CAG_nnn'CTG
Cuts at: 1347 1821 2257 3824 1347
Size: 474 436 1567 2475
Fragments arranged by size:
2475 1567 474 436
ApaLI G'TGCA_C
Cuts at: 250 718 3722 250
Size: 468 3004 1480
Fragments arranged by size:
3004 1480 468
ApoI r'AATT_y
Cuts at: 424 468 1956 4305 4561 424
Size: 44 1488 2349 256 815
Fragments arranged by size:
2349 1488 815 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
AvaI C'yCGr_G
Cuts at: 632 1972 632
Size: 1340 3612
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1708 1741 1708
Size: 33 4919
BamHI G'GATC_C
Cuts at: 1977 1977
Size: 4952
BanI G'GyrC_C
Cuts at: 182 1674 1968 2382 2630 2665 182
Size: 1492 294 414 248 35 2469
Fragments arranged by size:
2469 1492 414 294 248 35
BanII G_rGCy'C
Cuts at: 325 1508 1966 2996 325
Size: 1183 458 1030 2281
Fragments arranged by size:
2281 1183 1030 458
BbeI G_GCGC'C
Cuts at: 2386 2634 2386
Size: 248 4704
BbsI GAAGACnn'nnnn_
Cuts at: 789 789
Size: 4952
BbvCI CC'TCA_GC
Cuts at: 673 1580 673
Size: 907 4045
BciVI GTATCCnnnnn_n'
Cuts at: 2850 3611 4952 2850
Size: 761 1341 2850
Fragments arranged by size:
2850 1341 761
BclI T'GATC_A
Cuts at: 2472 2472
Size: 4952
BglI GCCn_nnn'nGGC
Cuts at: 112 139 169 112
Size: 27 30 4895
Fragments arranged by size:
4895 30 27
BglII A'GATC_T
Cuts at: 512 1184 2467 4504 512
Size: 672 1283 2037 960
Fragments arranged by size:
2037 1283 960 672
BlpI GC'TnA_GC
Cuts at: 206 2064 206
Size: 1858 3094
Bme1580I G_kGCm'C
Cuts at: 254 722 2577 2670 3726 254
Size: 468 1855 93 1056 1480
Fragments arranged by size:
1855 1480 1056 468 93
BmrI ACTGGGnnnn_n'
Cuts at: 1302 2164 2245 2580 4724 1302
Size: 862 81 335 2144 1530
Fragments arranged by size:
2144 1530 862 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 4952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 796 1045 1087 1147 1450 1810 394
Size: 402 249 42 60 303 360 3536
Fragments arranged by size:
3536 402 360 303 249 60 42
Bpu10I CC'TnA_GC
Cuts at: 116 673 1580 116
Size: 557 907 3488
Fragments arranged by size:
3488 907 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1060 2128 3007 3499 3797 598
Size: 462 1068 879 492 298 1753
Fragments arranged by size:
1753 1068 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 1739 4656 1739
Size: 2917 2035
BsaAI yAC'GTr
Cuts at: 1874 2305 1874
Size: 431 4521
BsaBI GATnn'nnATC
Cuts at: 6 511 2466 4503 6
Size: 505 1955 2037 455
Fragments arranged by size:
2037 1955 505 455
BsaHI Gr'CG_yC
Cuts at: 2383 2631 3333 4829 2383
Size: 248 702 1496 2506
Fragments arranged by size:
2506 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2141 2662 3340 3614 3761 4172 2141
Size: 521 678 274 147 411 2921
Fragments arranged by size:
2921 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 955 985 367
Size: 30 558 30 4334
Fragments arranged by size:
4334 558 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 661 661
Size: 4952
BseYI C'CCAG_C
Cuts at: 318 925 1260 1641 2377 3135 3712 4208 318
Size: 607 335 381 736 758 577 496 1062
Fragments arranged by size:
1062 758 736 607 577 496 381 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 889 1234 1918 553
Size: 336 345 684 3587
Fragments arranged by size:
3587 684 345 336
BsiEI CG_ry'CG
Cuts at: 104 173 2494 2540 3748 104
Size: 69 2321 46 1208 1308
Fragments arranged by size:
2321 1308 1208 69 46
BsiHKAI G_wGCw'C
Cuts at: 212 254 722 812 968 1133 1966 2744 2934
Size: 42 468 90 156 165 833 778 190
Cuts at: 2934 3726 212
Size: 792 1438
Fragments arranged by size:
1438 833 792 778 468 190 165 156
90 42
Bsp1286I G_dGCh'C
Cuts at: 212 254 325 722 812 968 1133 1508 1966
Size: 42 71 397 90 156 165 375 458
Cuts at: 1966 2577 2670 2744 2934 2996 3726 212
Size: 611 93 74 190 62 730 1438
Fragments arranged by size:
1438 730 611 458 397 375 190 165
156 93 90 74 71 62 42
BspEI T'CCGG_A
Cuts at: 4172 4172
Size: 4952
BspHI T'CATG_A
Cuts at: 4128 4128
Size: 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 831 2002 2216 2518 2899 320
Size: 511 1171 214 302 381 2373
Fragments arranged by size:
2373 1171 511 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3244 3298 4782 4947 50
Size: 3194 54 1484 165 55
Fragments arranged by size:
3194 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1255 2864 1255
Size: 1609 3343
BsrFI r'CCGG_y
Cuts at: 1015 1592 2950 3131 3340 1015
Size: 577 1358 181 209 2627
Fragments arranged by size:
2627 1358 577 209 181
BssHII G'CGCG_C
Cuts at: 361 361
Size: 4952
BssSI C'ACGA_G
Cuts at: 177 1612 3223 3581 177
Size: 1435 1611 358 1548
Fragments arranged by size:
1611 1548 1435 358
BstAPI GCAn_nnn'nTGC
Cuts at: 258 717 726 1008 1257 1821 2329 258
Size: 459 9 282 249 564 508 2881
Fragments arranged by size:
2881 564 508 459 282 249 9
BstBI TT'CG_AA
Cuts at: 413 3313 413
Size: 2900 2052
BstEII G'GTnAC_C
Cuts at: 816 816
Size: 4952
BstXI CCAn_nnnn'nTGG
Cuts at: 1944 1944
Size: 4952
BstYI r'GATC_y
Cuts at: 1 512 643 917 1184 1700 1977 2444 2467
Size: 511 131 274 267 516 277 467 23
Cuts at: 2467 2802 3048 4060 4146 4158 4504 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 516 511 467 449 346 335 277
274 267 246 131 86 23 12
BtgI C'CryG_G
Cuts at: 107 1169 1316 1772 107
Size: 1062 147 456 3287
Fragments arranged by size:
3287 1062 456 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2902 3083 3246 2902
Size: 181 163 4608
Fragments arranged by size:
4608 181 163
BtsI GCAGTG_nn'
Cuts at: 252 537 886 901 1470 1735 2195 252
Size: 285 349 15 569 265 460 3009
Fragments arranged by size:
3009 569 460 349 285 265 15
DraI TTT'AAA
Cuts at: 1951 1951
Size: 4952
DrdI GACnn_nn'nnGTC
Cuts at: 2658 3516 2658
Size: 858 4094
EaeI y'GGCC_r
Cuts at: 170 1934 2537 2711 3102 3129 4474 170
Size: 1764 603 174 391 27 1345 648
Fragments arranged by size:
1764 1345 648 603 391 174 27
EagI C'GGCC_G
Cuts at: 170 2537 170
Size: 2367 2585
EarI CTCTTCn'nnn_
Cuts at: 556 841 949 1231 1513 2975 3185 556
Size: 285 108 282 282 1462 210 2323
Fragments arranged by size:
2323 1462 285 282 282 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 229 3160 3470 3616 4762 229
Size: 2931 310 146 1146 419
Fragments arranged by size:
2931 1146 419 310 146
EcoICRI GAG'CTC
Cuts at: 1964 1964
Size: 4952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 796 886 1045 1087 1147 1450 1585 1810
Size: 402 90 159 42 60 303 135 225
Cuts at: 1810 2777 3209 3956 4224 394
Size: 967 432 747 268 1122
Fragments arranged by size:
1122 967 747 432 402 303 268 225
159 135 90 60 42
EcoNI CCTnn'n_nnAGG
Cuts at: 376 676 1027 376
Size: 300 351 4301
Fragments arranged by size:
4301 351 300
EcoO109I rG'GnC_Cy
Cuts at: 585 1053 1584 2091 585
Size: 468 531 507 3446
Fragments arranged by size:
3446 531 507 468
EcoRI G'AATT_C
Cuts at: 468 1956 468
Size: 1488 3464
EcoRV GAT'ATC
Cuts at: 392 392
Size: 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4952
FspI TGC'GCA
Cuts at: 158 2733 158
Size: 2575 2377
HaeII r_GCGC'y
Cuts at: 193 664 1177 2386 2634 3656 4635 193
Size: 471 513 1209 248 1022 979 510
Fragments arranged by size:
1209 1022 979 513 510 471 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1869 1901 2794 2824 2826 2856 577
Size: 32 1260 32 893 30 2 30 2673
Fragments arranged by size:
2673 1260 893 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1284 1991 1284
Size: 707 4245
KasI G'GCGC_C
Cuts at: 2382 2630 2382
Size: 248 4704
KpnI G_GTAC'C
Cuts at: 186 1972 186
Size: 1786 3166
MluI A'CGCG_T
Cuts at: 4385 4385
Size: 4952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1256 3623 3807 1256
Size: 2367 184 2401
Fragments arranged by size:
2401 2367 184
MscI TGG'CCA
Cuts at: 1936 2713 1936
Size: 777 4175
MslI CAynn'nnrTG
Cuts at: 911 971 973 1138 1672 2941 3068 911
Size: 60 2 165 534 1269 127 2795
Fragments arranged by size:
2795 1269 534 165 127 60 2
MspA1I CmG'CkG
Cuts at: 130 318 492 726 873 937 1065 1203 1248
Size: 188 174 234 147 64 128 138 45
Cuts at: 1248 1722 1821 2063 2377 2737 3750 3995 130
Size: 474 99 242 314 360 1013 245 1087
Fragments arranged by size:
1087 1013 474 360 314 245 242 234
188 174 147 138 128 99 64 45
NaeI GCC'GGC
Cuts at: 1017 3133 1017
Size: 2116 2836
NarI GG'CG_CC
Cuts at: 2383 2631 2383
Size: 248 4704
NcoI C'CATG_G
Cuts at: 107 1772 107
Size: 1665 3287
NgoMIV G'CCGG_C
Cuts at: 1015 3131 1015
Size: 2116 2836
NspI r_CATG'y
Cuts at: 972 1713 2005 2505 3412 972
Size: 741 292 500 907 2512
Fragments arranged by size:
2512 907 741 500 292
PciI A'CATG_T
Cuts at: 968 1709 3408 968
Size: 741 1699 2512
Fragments arranged by size:
2512 1699 741
PflMI CCAn_nnn'nTGG
Cuts at: 828 828
Size: 4952
PfoI T'CCnGG_A
Cuts at: 1703 1826 1703
Size: 123 4829
PmeI GTTT'AAAC
Cuts at: 1951 1951
Size: 4952
PmlI CAC'GTG
Cuts at: 1874 1874
Size: 4952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1850 1882 4091 4123 1850
Size: 32 2209 32 2679
Fragments arranged by size:
2679 2209 32 32
PpuMI rG'GwC_Cy
Cuts at: 585 1584 585
Size: 999 3953
PstI C_TGCA'G
Cuts at: 263 317 713 878 1093 1181 1849 1999 2684
Size: 54 396 165 215 88 668 150 685
Cuts at: 2684 263
Size: 2531
Fragments arranged by size:
2531 685 668 396 215 165 150 88
54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4952
PvuII CAG'CTG
Cuts at: 318 873 1065 1203 1248 1722 1821 2377 2737
Size: 555 192 138 45 474 99 556 360
Cuts at: 2737 318
Size: 2533
Fragments arranged by size:
2533 556 555 474 360 192 138 99
45
RsrII CG'GwC_CG
Cuts at: 3147 3147
Size: 4952
SacI G_AGCT'C
Cuts at: 1966 1966
Size: 4952
SalI G'TCGA_C
Cuts at: 1989 1989
Size: 4952
SapI GCTCTTCn'nnn_
Cuts at: 556 841 1231 1513 2975 3185 556
Size: 285 390 282 1462 210 2323
Fragments arranged by size:
2323 1462 390 285 282 210
SbfI CC_TGCA'GG
Cuts at: 263 1999 263
Size: 1736 3216
ScaI AGT'ACT
Cuts at: 4469 4469
Size: 4952
SexAI A'CCwGG_T
Cuts at: 587 986 587
Size: 399 4553
SfcI C'TryA_G
Cuts at: 33 259 313 709 874 1089 1177 1419 1845
Size: 226 54 396 165 215 88 242 426
Cuts at: 1845 1995 2680 3673 3864 33
Size: 150 685 993 191 1121
Fragments arranged by size:
1121 993 685 426 396 242 226 215
191 165 150 88 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 169 169
Size: 4952
SfoI GGC'GCC
Cuts at: 2384 2632 2384
Size: 248 4704
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
SmaI CCC'GGG
Cuts at: 634 1974 634
Size: 1340 3612
SmlI C'TyrA_G
Cuts at: 577 1075 2107 3022 3514 3776 577
Size: 498 1032 915 492 262 1753
Fragments arranged by size:
1753 1032 915 498 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4952
SphI G_CATG'C
Cuts at: 2005 2505 2005
Size: 500 4452
StuI AGG'CCT
Cuts at: 1843 1843
Size: 4952
StyI C'CwwG_G
Cuts at: 107 959 1772 2086 2440 107
Size: 852 813 314 354 2619
Fragments arranged by size:
2619 852 813 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 835 2535 2668 3127 3336 601
Size: 234 1700 133 459 209 2217
Fragments arranged by size:
2217 1700 459 234 209 133
TatI w'GTAC_w
Cuts at: 455 1040 1268 4467 455
Size: 585 228 3199 940
Fragments arranged by size:
3199 940 585 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 367 386 399 418 955 987 4469 4501 367
Size: 19 13 19 537 32 3482 32 818
Fragments arranged by size:
3482 818 537 32 32 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 2749 2749
Size: 4952
XbaI T'CTAG_A
Cuts at: 1983 1983
Size: 4952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1912 1929 2447 1912
Size: 17 518 4417
Fragments arranged by size:
4417 518 17
XmaI C'CCGG_G
Cuts at: 632 1972 632
Size: 1340 3612
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI AvrII BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI