Restriction map for
pF1KA0852
(Circular) (Six-base) MAPSORT of: pF1KA0852.seq Check: 5054 from: 1 to: 6023
pF1KA0852 6023 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1244 1244
Size: 6023
AatII G_ACGT'C
Cuts at: 607 607
Size: 6023
AccI GT'mk_AC
Cuts at: 202 1746 3061 202
Size: 1544 1315 3164
Fragments arranged by size:
3164 1544 1315
Acc65I G'GTAC_C
Cuts at: 3039 3039
Size: 6023
AclI AA'CG_TT
Cuts at: 5863 5863
Size: 6023
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 1639 1711 2782 3848 4280 5027 5295 848
Size: 791 72 1071 1066 432 747 268 1576
Fragments arranged by size:
1576 1071 1066 791 747 432 268 72
AflIII A'CryG_T
Cuts at: 758 3375 4479 5456 758
Size: 2617 1104 977 1325
Fragments arranged by size:
2617 1325 1104 977
AgeI A'CCGG_T
Cuts at: 1523 4411 1523
Size: 2888 3135
AleI CACnn'nnGTG
Cuts at: 2362 2362
Size: 6023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2741 2741
Size: 6023
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1051 1083 1051
Size: 32 5991
AlwNI CAG_nnn'CTG
Cuts at: 2004 2149 2910 3328 4895 2004
Size: 145 761 418 1567 3132
Fragments arranged by size:
3132 1567 761 418 145
ApaI G_GGCC'C
Cuts at: 1117 2545 1117
Size: 1428 4595
ApaLI G'TGCA_C
Cuts at: 4793 4793
Size: 6023
ApoI r'AATT_y
Cuts at: 412 1020 1622 2733 3027 5376 5632 412
Size: 608 602 1111 294 2349 256 803
Fragments arranged by size:
2349 1111 803 608 602 294 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6023
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6023
AvaI C'yCGr_G
Cuts at: 383 1895 2039 2834 3043 383
Size: 1512 144 795 209 3363
Fragments arranged by size:
3363 1512 795 209 144
AvrII C'CTAG_G
Cuts at: 879 879
Size: 6023
BamHI G'GATC_C
Cuts at: 141 3048 141
Size: 2907 3116
BanI G'GyrC_C
Cuts at: 1232 1938 3039 3453 3701 3736 1232
Size: 706 1101 414 248 35 3519
Fragments arranged by size:
3519 1101 706 414 248 35
BanII G_rGCy'C
Cuts at: 244 1117 2038 2249 2295 2545 2579 2795 3037
Size: 873 921 211 46 250 34 216 242
Cuts at: 3037 4067 244
Size: 1030 2200
Fragments arranged by size:
2200 1030 921 873 250 242 216 211
46 34
BbeI G_GCGC'C
Cuts at: 3457 3705 3457
Size: 248 5775
BbsI GAAGACnn'nnnn_
Cuts at: 293 1591 2092 293
Size: 1298 501 4224
Fragments arranged by size:
4224 1298 501
BbvCI CC'TCA_GC
Cuts at: 1751 2911 1751
Size: 1160 4863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5989
BciVI GTATCCnnnnn_n'
Cuts at: 3921 4682 6023 3921
Size: 761 1341 3921
Fragments arranged by size:
3921 1341 761
BclI T'GATC_A
Cuts at: 518 1091 1784 3543 518
Size: 573 693 1759 2998
Fragments arranged by size:
2998 1759 693 573
BglI GCCn_nnn'nGGC
Cuts at: 630 2557 630
Size: 1927 4096
BglII A'GATC_T
Cuts at: 3538 5575 3538
Size: 2037 3986
BlpI GC'TnA_GC
Cuts at: 3135 3135
Size: 6023
Bme1580I G_kGCm'C
Cuts at: 619 701 1117 1172 2417 2545 3648 3741 4797
Size: 82 416 55 1245 128 1103 93 1056
Cuts at: 4797 619
Size: 1845
Fragments arranged by size:
1845 1245 1103 1056 416 128 93 82
55
BmgBI CAC'GTC
Cuts at: 299 761 299
Size: 462 5561
BmrI ACTGGGnnnn_n'
Cuts at: 474 3235 3316 3651 5795 474
Size: 2761 81 335 2144 702
Fragments arranged by size:
2761 2144 702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 651 875 4390 651
Size: 224 3515 2284
Fragments arranged by size:
3515 2284 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 1204 1666 1909 2057 888
Size: 316 462 243 148 4854
Fragments arranged by size:
4854 462 316 243 148
Bpu10I CC'TnA_GC
Cuts at: 1751 2623 2911 1751
Size: 872 288 4863
Fragments arranged by size:
4863 872 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3199 4078 4570 4868 2269
Size: 930 879 492 298 3424
Fragments arranged by size:
3424 930 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 881 2429 2632 5727 881
Size: 1548 203 3095 1177
Fragments arranged by size:
3095 1548 1177 203
BsaAI yAC'GTr
Cuts at: 813 2316 2412 3376 813
Size: 1503 96 964 3460
Fragments arranged by size:
3460 1503 964 96
BsaBI GATnn'nnATC
Cuts at: 6 682 688 3537 5574 6
Size: 676 6 2849 2037 455
Fragments arranged by size:
2849 2037 676 455 6
BsaHI Gr'CG_yC
Cuts at: 604 2984 3454 3702 4404 5900 604
Size: 2380 470 248 702 1496 727
Fragments arranged by size:
2380 1496 727 702 470 248
BsaWI w'CCGG_w
Cuts at: 1523 2490 2666 3212 3733 4411 4685 4832 5243
Size: 967 176 546 521 678 274 147 411
Cuts at: 5243 1523
Size: 2303
Fragments arranged by size:
2303 967 678 546 521 411 274 176
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 1915 2182 2196 2545 2548 2908 478
Size: 1437 267 14 349 3 360 3593
Fragments arranged by size:
3593 1437 360 349 267 14 3
BseYI C'CCAG_C
Cuts at: 1798 2260 2441 2552 3448 4206 4783 5279 1798
Size: 462 181 111 896 758 577 496 2542
Fragments arranged by size:
2542 896 758 577 496 462 181 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 2017 2296 2965 727
Size: 1290 279 669 3785
Fragments arranged by size:
3785 1290 669 279
BsiEI CG_ry'CG
Cuts at: 104 1765 3565 3611 4819 104
Size: 1661 1800 46 1208 1308
Fragments arranged by size:
1800 1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 662 812 999 1259 2249 2295 2629 3037 3815
Size: 150 187 260 990 46 334 408 778
Cuts at: 3815 4005 4797 662
Size: 190 792 1888
Fragments arranged by size:
1888 990 792 778 408 334 260 190
187 150 46
BsmI GAATG_Cn'
Cuts at: 378 2596 378
Size: 2218 3805
BsmBI CGTCTCn'nnnn_
Cuts at: 595 2881 595
Size: 2286 3737
Bsp1286I G_dGCh'C
Cuts at: 244 619 662 701 812 999 1117 1172 1259
Size: 375 43 39 111 187 118 55 87
Cuts at: 1259 2038 2249 2295 2417 2545 2579 2629 2795
Size: 779 211 46 122 128 34 50 166
Cuts at: 2795 3037 3648 3741 3815 4005 4067 4797 244
Size: 242 611 93 74 190 62 730 1470
Fragments arranged by size:
1470 779 730 611 375 242 211 190
187 166 128 122 118 111 93 87
74 62 55 50 46 43 39 34
BspEI T'CCGG_A
Cuts at: 2490 2666 5243 2490
Size: 176 2577 3270
Fragments arranged by size:
3270 2577 176
BspHI T'CATG_A
Cuts at: 324 5199 324
Size: 4875 1148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1244 1785 1992 2657 3073 3287 3589 3970 1244
Size: 541 207 665 416 214 302 381 3297
Fragments arranged by size:
3297 665 541 416 381 302 214 207
BsrBI CCG'CTC
Cuts at: 50 630 1676 4315 4369 5853 6018 50
Size: 580 1046 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 1046 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1253 3935 1253
Size: 2682 3341
BsrFI r'CCGG_y
Cuts at: 1229 1523 4021 4202 4411 1229
Size: 294 2498 181 209 2841
Fragments arranged by size:
2841 2498 294 209 181
BsrGI T'GTAC_A
Cuts at: 734 749 734
Size: 15 6008
BssSI C'ACGA_G
Cuts at: 2635 4294 4652 2635
Size: 1659 358 4006
Fragments arranged by size:
4006 1659 358
BstAPI GCAn_nnn'nTGC
Cuts at: 705 823 2385 2943 3400 705
Size: 118 1562 558 457 3328
Fragments arranged by size:
3328 1562 558 457 118
BstBI TT'CG_AA
Cuts at: 4384 4384
Size: 6023
BstXI CCAn_nnnn'nTGG
Cuts at: 2925 2925
Size: 6023
BstYI r'GATC_y
Cuts at: 1 141 683 1053 2660 3048 3515 3538 3873
Size: 140 542 370 1607 388 467 23 335
Cuts at: 3873 4119 5131 5217 5229 5575 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1607 1012 542 467 449 388 370 346
335 246 140 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1010 1934 1010
Size: 924 5099
BtgI C'CryG_G
Cuts at: 693 2363 693
Size: 1670 4353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1272 3973 4154 4317 1272
Size: 2701 181 163 2978
Fragments arranged by size:
2978 2701 181 163
BtsI GCAGTG_nn'
Cuts at: 358 2463 2615 3266 358
Size: 2105 152 651 3115
Fragments arranged by size:
3115 2105 651 152
DraI TTT'AAA
Cuts at: 3022 3022
Size: 6023
DraIII CAC_nnn'GTG
Cuts at: 705 705
Size: 6023
DrdI GACnn_nn'nnGTC
Cuts at: 1754 2653 3729 4587 1754
Size: 899 1076 858 3190
Fragments arranged by size:
3190 1076 899 858
EaeI y'GGCC_r
Cuts at: 182 3608 3782 4173 4200 5545 182
Size: 3426 174 391 27 1345 660
Fragments arranged by size:
3426 1345 660 391 174 27
EagI C'GGCC_G
Cuts at: 3608 3608
Size: 6023
EarI CTCTTCn'nnn_
Cuts at: 310 824 964 1589 2261 4046 4256 310
Size: 514 140 625 672 1785 210 2077
Fragments arranged by size:
2077 1785 672 625 514 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4231 4541 4687 5833 4231
Size: 310 146 1146 4421
Fragments arranged by size:
4421 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2247 2293 3035 2247
Size: 46 742 5235
Fragments arranged by size:
5235 742 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 888 1204 1639 1666 1711 1909 2057 2782
Size: 40 316 435 27 45 198 148 725
Cuts at: 2782 3848 4280 5027 5295 848
Size: 1066 432 747 268 1576
Fragments arranged by size:
1576 1066 747 725 435 432 316 268
198 148 45 40 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2871 2938 2871
Size: 67 5956
EcoO109I rG'GnC_Cy
Cuts at: 1650 2542 2997 3162 1650
Size: 892 455 165 4511
Fragments arranged by size:
4511 892 455 165
EcoRI G'AATT_C
Cuts at: 2733 3027 2733
Size: 294 5729
EcoRV GAT'ATC
Cuts at: 588 588
Size: 6023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2787 4042 2787
Size: 1255 4768
FspI TGC'GCA
Cuts at: 250 949 2863 3804 250
Size: 699 1914 941 2469
Fragments arranged by size:
2469 1914 941 699
FspAI rTGC'GCAy
Cuts at: 250 250
Size: 6023
HaeII r_GCGC'y
Cuts at: 636 3457 3705 4727 5706 636
Size: 2821 248 1022 979 953
Fragments arranged by size:
2821 1022 979 953 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 2616 2648 2734 2766 3865 3895 3897
Size: 32 2028 32 86 32 1099 30 2
Cuts at: 3897 3927 556
Size: 30 2652
Fragments arranged by size:
2652 2028 1099 86 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1038 3062 1038
Size: 2024 3999
KasI G'GCGC_C
Cuts at: 3453 3701 3453
Size: 248 5775
KpnI G_GTAC'C
Cuts at: 3043 3043
Size: 6023
MluI A'CGCG_T
Cuts at: 5456 5456
Size: 6023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2107 4694 4878 2107
Size: 2587 184 3252
Fragments arranged by size:
3252 2587 184
MscI TGG'CCA
Cuts at: 3784 3784
Size: 6023
MslI CAynn'nnrTG
Cuts at: 2362 4012 4139 2362
Size: 1650 127 4246
Fragments arranged by size:
4246 1650 127
MspA1I CmG'CkG
Cuts at: 506 655 1122 1455 2441 2721 3134 3448 3808
Size: 149 467 333 986 280 413 314 360
Cuts at: 3808 4821 5066 506
Size: 1013 245 1463
Fragments arranged by size:
1463 1013 986 467 413 360 333 314
280 245 149
NaeI GCC'GGC
Cuts at: 4204 4204
Size: 6023
NarI GG'CG_CC
Cuts at: 3454 3702 3454
Size: 248 5775
NcoI C'CATG_G
Cuts at: 693 693
Size: 6023
NgoMIV G'CCGG_C
Cuts at: 4202 4202
Size: 6023
NspI r_CATG'y
Cuts at: 1068 1142 2383 3076 3576 4483 1068
Size: 74 1241 693 500 907 2608
Fragments arranged by size:
2608 1241 907 693 500 74
PasI CC'CwG_GG
Cuts at: 1560 1560
Size: 6023
PciI A'CATG_T
Cuts at: 4479 4479
Size: 6023
PfoI T'CCnGG_A
Cuts at: 1181 1181
Size: 6023
PmeI GTTT'AAAC
Cuts at: 3022 3022
Size: 6023
PmlI CAC'GTG
Cuts at: 2316 2316
Size: 6023
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5162 5194 5162
Size: 32 5991
PpuMI rG'GwC_Cy
Cuts at: 2997 2997
Size: 6023
PspOMI G'GGCC_C
Cuts at: 1113 2541 1113
Size: 1428 4595
PstI C_TGCA'G
Cuts at: 2906 3070 3755 2906
Size: 164 685 5174
Fragments arranged by size:
5174 685 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6023
PvuII CAG'CTG
Cuts at: 1122 1455 2721 3448 3808 1122
Size: 333 1266 727 360 3337
Fragments arranged by size:
3337 1266 727 360 333
RsrII CG'GwC_CG
Cuts at: 4218 4218
Size: 6023
SacI G_AGCT'C
Cuts at: 2249 2295 3037 2249
Size: 46 742 5235
Fragments arranged by size:
5235 742 46
SalI G'TCGA_C
Cuts at: 3060 3060
Size: 6023
SapI GCTCTTCn'nnn_
Cuts at: 4046 4256 4046
Size: 210 5813
SbfI CC_TGCA'GG
Cuts at: 3070 3070
Size: 6023
ScaI AGT'ACT
Cuts at: 445 2770 5540 445
Size: 2325 2770 928
Fragments arranged by size:
2770 2325 928
SexAI A'CCwGG_T
Cuts at: 1175 1175
Size: 6023
SfcI C'TryA_G
Cuts at: 33 1080 2902 3066 3751 4744 4935 33
Size: 1047 1822 164 685 993 191 1121
Fragments arranged by size:
1822 1121 1047 993 685 191 164
SfiI GGCCn_nnn'nGGCC
Cuts at: 2557 2557
Size: 6023
SfoI GGC'GCC
Cuts at: 3455 3703 3455
Size: 248 5775
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6023
SmaI CCC'GGG
Cuts at: 385 1897 2836 3045 385
Size: 1512 939 209 3363
Fragments arranged by size:
3363 1512 939 209
SmlI C'TyrA_G
Cuts at: 2284 3178 4093 4585 4847 2284
Size: 894 915 492 262 3460
Fragments arranged by size:
3460 915 894 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6023
SphI G_CATG'C
Cuts at: 3076 3576 3076
Size: 500 5523
StuI AGG'CCT
Cuts at: 2845 2845
Size: 6023
StyI C'CwwG_G
Cuts at: 693 879 1220 1703 2840 3008 3157 3511 693
Size: 186 341 483 1137 168 149 354 3205
Fragments arranged by size:
3205 1137 483 354 341 186 168 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1683 1751 2112 2433 3606 3739 4198 4407 1683
Size: 68 361 321 1173 133 459 209 3299
Fragments arranged by size:
3299 1173 459 361 321 209 133 68
TatI w'GTAC_w
Cuts at: 443 734 749 755 2768 5538 443
Size: 291 15 6 2013 2770 928
Fragments arranged by size:
2770 2013 928 291 15 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 2121 2153 5540 5572 500
Size: 32 1589 32 3387 32 951
Fragments arranged by size:
3387 1589 951 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2091 3820 2091
Size: 1729 4294
XbaI T'CTAG_A
Cuts at: 3054 3054
Size: 6023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 365 2438 3518 365
Size: 2073 1080 2870
Fragments arranged by size:
2870 2073 1080
XmaI C'CCGG_G
Cuts at: 383 1895 2834 3043 383
Size: 1512 939 209 3363
Fragments arranged by size:
3363 1512 939 209
ZraI GAC'GTC
Cuts at: 605 605
Size: 6023
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI BaeI BfrBI BmtI BsaXI
BsiWI BssHII BstEII BstZ17I ClaI CspCI FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PshAI PsiI PspXI PsrI SacII SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI