Restriction map for
pF1KA0838
(Circular) (Six-base) MAPSORT of: pF1KA0838.seq Check: 4018 from: 1 to: 5120
pF1KA0838 5120 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2158 2158
Size: 5120
Acc65I G'GTAC_C
Cuts at: 2136 2136
Size: 5120
AclI AA'CG_TT
Cuts at: 1237 4960 1237
Size: 3723 1397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 943 1478 2945 3377 3730 4392 572
Size: 371 535 1467 432 353 662 1300
Fragments arranged by size:
1467 1300 662 535 432 371 353
AflIII A'CryG_T
Cuts at: 446 2472 4272 4553 446
Size: 2026 1800 281 1013
Fragments arranged by size:
2026 1800 1013 281
AhdI GACnn_n'nnGTC
Cuts at: 780 780
Size: 5120
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 606 638 606
Size: 32 5088
AlwNI CAG_nnn'CTG
Cuts at: 354 510 2425 3863 354
Size: 156 1915 1438 1611
Fragments arranged by size:
1915 1611 1438 156
ApaI G_GGCC'C
Cuts at: 250 250
Size: 5120
ApaLI G'TGCA_C
Cuts at: 3958 3958
Size: 5120
ApoI r'AATT_y
Cuts at: 580 871 1168 1269 1649 1915 2018 2124 4473
Size: 291 297 101 380 266 103 106 2349
Cuts at: 4473 4729 580
Size: 256 971
Fragments arranged by size:
2349 971 380 297 291 266 256 106
103 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 126 221 382 433 2140 126
Size: 95 161 51 1707 3106
Fragments arranged by size:
3106 1707 161 95 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 929 962 929
Size: 33 5087
BamHI G'GATC_C
Cuts at: 2145 2145
Size: 5120
BanI G'GyrC_C
Cuts at: 411 2136 2550 2798 2833 411
Size: 1725 414 248 35 2698
Fragments arranged by size:
2698 1725 414 248 35
BanII G_rGCy'C
Cuts at: 250 308 1456 2134 3164 250
Size: 58 1148 678 1030 2206
Fragments arranged by size:
2206 1148 1030 678 58
BbeI G_GCGC'C
Cuts at: 415 2554 2802 415
Size: 2139 248 2733
Fragments arranged by size:
2733 2139 248
BbsI GAAGACnn'nnnn_
Cuts at: 949 1531 949
Size: 582 4538
BciVI GTATCCnnnnn_n'
Cuts at: 3018 4074 5120 3018
Size: 1056 1046 3018
Fragments arranged by size:
3018 1056 1046
BclI T'GATC_A
Cuts at: 1749 2640 1749
Size: 891 4229
BfrBI ATG'CAT
Cuts at: 1488 1488
Size: 5120
BglI GCCn_nnn'nGGC
Cuts at: 409 409
Size: 5120
BglII A'GATC_T
Cuts at: 341 699 2635 4672 341
Size: 358 1936 2037 789
Fragments arranged by size:
2037 1936 789 358
BlpI GC'TnA_GC
Cuts at: 2232 2232
Size: 5120
Bme1580I G_kGCm'C
Cuts at: 192 250 2745 2838 3962 192
Size: 58 2495 93 1124 1350
Fragments arranged by size:
2495 1350 1124 93 58
BmrI ACTGGGnnnn_n'
Cuts at: 2332 2413 2748 4892 2332
Size: 81 335 2144 2560
Fragments arranged by size:
2560 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 194 3487 194
Size: 3293 1827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 953 1136 1574 1817 854
Size: 99 183 438 243 4157
Fragments arranged by size:
4157 438 243 183 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2296 3175 3889 4187 2036
Size: 260 879 714 298 2969
Fragments arranged by size:
2969 879 714 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 1616 4824 1616
Size: 3208 1912
BsaAI yAC'GTr
Cuts at: 2473 2473
Size: 5120
BsaBI GATnn'nnATC
Cuts at: 6 2634 4671 6
Size: 2628 2037 455
Fragments arranged by size:
2628 2037 455
BsaHI Gr'CG_yC
Cuts at: 216 412 2551 2799 3501 4997 216
Size: 196 2139 248 702 1496 339
Fragments arranged by size:
2139 1496 702 339 248 196
BsaWI w'CCGG_w
Cuts at: 2309 2830 3508 3919 4066 4340 2309
Size: 521 678 411 147 274 3089
Fragments arranged by size:
3089 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 827 857 1109 1139 827
Size: 30 252 30 4808
Fragments arranged by size:
4808 252 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1604 1607 1604
Size: 3 5117
BseYI C'CCAG_C
Cuts at: 354 1561 2545 3303 3968 4376 354
Size: 1207 984 758 665 408 1098
Fragments arranged by size:
1207 1098 984 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1793 1975 1793
Size: 182 4938
BsiEI CG_ry'CG
Cuts at: 104 157 238 242 2662 2708 3938 104
Size: 53 81 4 2420 46 1230 1286
Fragments arranged by size:
2420 1286 1230 81 53 46 4
BsiHKAI G_wGCw'C
Cuts at: 370 1363 2134 2912 3102 3962 370
Size: 993 771 778 190 860 1528
Fragments arranged by size:
1528 993 860 778 771 190
BsmI GAATG_Cn'
Cuts at: 924 1488 1568 924
Size: 564 80 4476
Fragments arranged by size:
4476 564 80
BsmBI CGTCTCn'nnnn_
Cuts at: 433 433
Size: 5120
Bsp1286I G_dGCh'C
Cuts at: 192 250 308 370 1363 1456 2134 2745 2838
Size: 58 58 62 993 93 678 611 93
Cuts at: 2838 2912 3102 3164 3962 192
Size: 74 190 62 798 1350
Fragments arranged by size:
1350 993 798 678 611 190 93 93
74 62 62 58 58
BspHI T'CATG_A
Cuts at: 3552 3552
Size: 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 2170 2384 2686 3067 152
Size: 2018 214 302 381 2205
Fragments arranged by size:
2205 2018 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3412 3466 4950 5115 50
Size: 3362 54 1484 165 55
Fragments arranged by size:
3362 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 989 3032 989
Size: 2043 3077
BsrFI r'CCGG_y
Cuts at: 163 212 237 297 3118 3299 163
Size: 49 25 60 2821 181 1984
Fragments arranged by size:
2821 1984 181 60 49 25
BsrGI T'GTAC_A
Cuts at: 904 904
Size: 5120
BssSI C'ACGA_G
Cuts at: 3391 4099 3391
Size: 708 4412
BstAPI GCAn_nnn'nTGC
Cuts at: 2497 2497
Size: 5120
BstBI TT'CG_AA
Cuts at: 1467 1815 3481 1467
Size: 348 1666 3106
Fragments arranged by size:
3106 1666 348
BstEII G'GTnAC_C
Cuts at: 201 201
Size: 5120
BstXI CCAn_nnnn'nTGG
Cuts at: 1395 1593 1395
Size: 198 4922
BstYI r'GATC_y
Cuts at: 1 341 699 2145 2612 2635 2970 3216 3522
Size: 340 358 1446 467 23 335 246 306
Cuts at: 3522 3534 3620 4672 1
Size: 12 86 1052 449
Fragments arranged by size:
1446 1052 467 449 358 340 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 483 483
Size: 5120
BtgI C'CryG_G
Cuts at: 242 274 1976 242
Size: 32 1702 3386
Fragments arranged by size:
3386 1702 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3070 3251 3414 3070
Size: 181 163 4776
Fragments arranged by size:
4776 181 163
BtsI GCAGTG_nn'
Cuts at: 1987 2363 1987
Size: 376 4744
ClaI AT'CG_AT
Cuts at: 1026 1026
Size: 5120
DraI TTT'AAA
Cuts at: 708 2016 2119 708
Size: 1308 103 3709
Fragments arranged by size:
3709 1308 103
DraIII CAC_nnn'GTG
Cuts at: 209 209
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 2826 4170 2826
Size: 1344 3776
EaeI y'GGCC_r
Cuts at: 235 239 295 866 2705 2879 3270 3297 4642
Size: 4 56 571 1839 174 391 27 1345
Cuts at: 4642 235
Size: 713
Fragments arranged by size:
1839 1345 713 571 391 174 56 27
4
EagI C'GGCC_G
Cuts at: 235 239 2705 235
Size: 4 2466 2650
Fragments arranged by size:
2650 2466 4
EarI CTCTTCn'nnn_
Cuts at: 668 1731 3143 3353 668
Size: 1063 1412 210 2435
Fragments arranged by size:
2435 1412 1063 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 316 3328 4070 4216 4930 316
Size: 3012 742 146 714 506
Fragments arranged by size:
3012 742 714 506 146
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 854 943 953 1136 1478 1574 1817 2945
Size: 282 89 10 183 342 96 243 1128
Cuts at: 2945 3377 3730 4392 572
Size: 432 353 662 1300
Fragments arranged by size:
1300 1128 662 432 353 342 282 243
183 96 89 10
EcoO109I rG'GnC_Cy
Cuts at: 141 318 2259 141
Size: 177 1941 3002
Fragments arranged by size:
3002 1941 177
EcoRI G'AATT_C
Cuts at: 1649 2124 1649
Size: 475 4645
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1841 3139 1841
Size: 1298 3822
FseI GG_CCGG'CC
Cuts at: 241 241
Size: 5120
FspI TGC'GCA
Cuts at: 2901 2901
Size: 5120
HaeII r_GCGC'y
Cuts at: 415 2554 2802 4032 4803 415
Size: 2139 248 1230 771 732
Fragments arranged by size:
2139 1230 771 732 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 766 798 2962 2992 2994 3024 357
Size: 32 377 32 2164 30 2 30 2453
Fragments arranged by size:
2453 2164 377 32 32 30 30 2
HincII GTy'rAC
Cuts at: 740 2159 740
Size: 1419 3701
KasI G'GCGC_C
Cuts at: 411 2550 2798 411
Size: 2139 248 2733
Fragments arranged by size:
2733 2139 248
KpnI G_GTAC'C
Cuts at: 2140 2140
Size: 5120
MfeI C'AATT_G
Cuts at: 545 1121 545
Size: 576 4544
MluI A'CGCG_T
Cuts at: 446 4553 446
Size: 4107 1013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1014 1200 3879 4063 1014
Size: 186 2679 184 2071
Fragments arranged by size:
2679 2071 186 184
MscI TGG'CCA
Cuts at: 868 2881 868
Size: 2013 3107
MslI CAynn'nnrTG
Cuts at: 800 1028 1324 1369 1493 1694 1975 2006 3109
Size: 228 296 45 124 201 281 31 1103
Cuts at: 3109 3236 800
Size: 127 2684
Fragments arranged by size:
2684 1103 296 281 228 201 127 124
45 31
MspA1I CmG'CkG
Cuts at: 244 400 1356 1851 2231 2545 2905 3689 3934
Size: 156 956 495 380 314 360 784 245
Cuts at: 3934 244
Size: 1430
Fragments arranged by size:
1430 956 784 495 380 360 314 245
156
NaeI GCC'GGC
Cuts at: 165 214 239 299 3301 165
Size: 49 25 60 3002 1984
Fragments arranged by size:
3002 1984 60 49 25
NarI GG'CG_CC
Cuts at: 412 2551 2799 412
Size: 2139 248 2733
Fragments arranged by size:
2733 2139 248
NcoI C'CATG_G
Cuts at: 1976 1976
Size: 5120
NgoMIV G'CCGG_C
Cuts at: 163 212 237 297 3299 163
Size: 49 25 60 3002 1984
Fragments arranged by size:
3002 1984 60 49 25
NsiI A_TGCA'T
Cuts at: 1490 1490
Size: 5120
NspI r_CATG'y
Cuts at: 1503 2173 2673 4276 1503
Size: 670 500 1603 2347
Fragments arranged by size:
2347 1603 670 500
PciI A'CATG_T
Cuts at: 4272 4272
Size: 5120
PflMI CCAn_nnn'nTGG
Cuts at: 1435 1435
Size: 5120
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 5088
PpuMI rG'GwC_Cy
Cuts at: 141 141
Size: 5120
PspOMI G'GGCC_C
Cuts at: 246 246
Size: 5120
PstI C_TGCA'G
Cuts at: 1893 2167 2852 1893
Size: 274 685 4161
Fragments arranged by size:
4161 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 1356 2545 2905 1356
Size: 1189 360 3571
Fragments arranged by size:
3571 1189 360
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5120
SacII CC_GC'GG
Cuts at: 245 245
Size: 5120
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5120
SapI GCTCTTCn'nnn_
Cuts at: 3143 3353 3143
Size: 210 4910
SbfI CC_TGCA'GG
Cuts at: 2167 2167
Size: 5120
ScaI AGT'ACT
Cuts at: 1448 4637 1448
Size: 3189 1931
SfcI C'TryA_G
Cuts at: 33 602 1889 2163 2848 3816 4007 33
Size: 569 1287 274 685 968 191 1146
Fragments arranged by size:
1287 1146 968 685 569 274 191
SfoI GGC'GCC
Cuts at: 413 2552 2800 413
Size: 2139 248 2733
Fragments arranged by size:
2733 2139 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SgrAI Cr'CCGG_yG
Cuts at: 163 163
Size: 5120
SmaI CCC'GGG
Cuts at: 384 435 2142 384
Size: 51 1707 3362
Fragments arranged by size:
3362 1707 51
SmlI C'TyrA_G
Cuts at: 2051 2275 3190 3904 4166 2051
Size: 224 915 714 262 3005
Fragments arranged by size:
3005 915 714 262 224
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 2173 2673 2173
Size: 500 4620
StyI C'CwwG_G
Cuts at: 197 422 1952 1976 2254 2608 197
Size: 225 1530 24 278 354 2709
Fragments arranged by size:
2709 1530 354 278 225 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2703 2836 3295 3504 2703
Size: 133 459 209 4319
Fragments arranged by size:
4319 459 209 133
TatI w'GTAC_w
Cuts at: 904 1446 2042 4635 904
Size: 542 596 2593 1389
Fragments arranged by size:
2593 1389 596 542
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4637 4669 4637
Size: 32 5088
Tth111I GACn'n_nGTC
Cuts at: 1177 2917 1177
Size: 1740 3380
XbaI T'CTAG_A
Cuts at: 1864 2151 1864
Size: 287 4833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 2615
Size: 5120
XmaI C'CCGG_G
Cuts at: 382 433 2140 382
Size: 51 1707 3362
Fragments arranged by size:
3362 1707 51
XmnI GAAnn'nnTTC
Cuts at: 1463 1463
Size: 5120
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
AvrII BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BspEI
BssHII BstZ17I CspCI EcoNI EcoRV FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI ZraI