Restriction map for
pF1KA0837
(Circular) (Six-base) MAPSORT of: pF1KA0837.seq Check: 551 from: 1 to: 5279
pF1KA0837 5279 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2317 2317
Size: 5279
Acc65I G'GTAC_C
Cuts at: 2295 2295
Size: 5279
AclI AA'CG_TT
Cuts at: 5119 5119
Size: 5279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1299 2018 2092 3104 3536 4283 4551 1299
Size: 719 74 1012 432 747 268 2027
Fragments arranged by size:
2027 1012 747 719 432 268 74
AfeI AGC'GCT
Cuts at: 1342 1342
Size: 5279
AflIII A'CryG_T
Cuts at: 1154 2165 2631 3735 4712 1154
Size: 1011 466 1104 977 1721
Fragments arranged by size:
1721 1104 1011 977 466
AgeI A'CCGG_T
Cuts at: 3667 3667
Size: 5279
AleI CACnn'nnGTG
Cuts at: 271 271
Size: 5279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2002 2070 2002
Size: 68 5211
AlwNI CAG_nnn'CTG
Cuts at: 543 2584 4151 543
Size: 2041 1567 1671
Fragments arranged by size:
2041 1671 1567
ApaI G_GGCC'C
Cuts at: 503 730 2019 503
Size: 227 1289 3763
Fragments arranged by size:
3763 1289 227
ApaLI G'TGCA_C
Cuts at: 1569 4049 1569
Size: 2480 2799
ApoI r'AATT_y
Cuts at: 574 2283 4632 4888 574
Size: 1709 2349 256 965
Fragments arranged by size:
2349 1709 965 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5279
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5279
AvaI C'yCGr_G
Cuts at: 1891 2299 1891
Size: 408 4871
BamHI G'GATC_C
Cuts at: 2304 2304
Size: 5279
BanI G'GyrC_C
Cuts at: 283 1535 1626 2295 2709 2957 2992 283
Size: 1252 91 669 414 248 35 2570
Fragments arranged by size:
2570 1252 669 414 248 91 35
BanII G_rGCy'C
Cuts at: 139 503 730 2019 2293 3323 139
Size: 364 227 1289 274 1030 2095
Fragments arranged by size:
2095 1289 1030 364 274 227
BbeI G_GCGC'C
Cuts at: 1630 2713 2961 1630
Size: 1083 248 3948
Fragments arranged by size:
3948 1083 248
BbvCI CC'TCA_GC
Cuts at: 419 419
Size: 5279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5245
BciVI GTATCCnnnnn_n'
Cuts at: 200 3177 3938 5279 200
Size: 2977 761 1341 200
Fragments arranged by size:
2977 1341 761 200
BclI T'GATC_A
Cuts at: 621 851 2799 621
Size: 230 1948 3101
Fragments arranged by size:
3101 1948 230
BglI GCCn_nnn'nGGC
Cuts at: 568 568
Size: 5279
BglII A'GATC_T
Cuts at: 1310 2049 2794 4831 1310
Size: 739 745 2037 1758
Fragments arranged by size:
2037 1758 745 739
BlpI GC'TnA_GC
Cuts at: 481 2391 481
Size: 1910 3369
Bme1580I G_kGCm'C
Cuts at: 288 393 503 730 974 1573 1619 2019 2904
Size: 105 110 227 244 599 46 400 885
Cuts at: 2904 2997 4053 288
Size: 93 1056 1514
Fragments arranged by size:
1514 1056 885 599 400 244 227 110
105 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 1689 2491 2572 2907 5051 1689
Size: 802 81 335 2144 1917
Fragments arranged by size:
2144 1917 802 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2277 3646 2277
Size: 1369 3910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 580 821 1372 1502 1817 196
Size: 384 241 551 130 315 3658
Fragments arranged by size:
3658 551 384 315 241 130
Bpu10I CC'TnA_GC
Cuts at: 221 419 524 1871 2219 221
Size: 198 105 1347 348 3281
Fragments arranged by size:
3281 1347 348 198 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 2455 3334 3826 4124 829
Size: 1626 879 492 298 1984
Fragments arranged by size:
1984 1626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2208 4983 2208
Size: 2775 2504
BsaAI yAC'GTr
Cuts at: 1620 2632 1620
Size: 1012 4267
BsaBI GATnn'nnATC
Cuts at: 6 2793 4830 6
Size: 2787 2037 455
Fragments arranged by size:
2787 2037 455
BsaHI Gr'CG_yC
Cuts at: 1627 2710 2958 3660 5156 1627
Size: 1083 248 702 1496 1750
Fragments arranged by size:
1750 1496 1083 702 248
BsaWI w'CCGG_w
Cuts at: 586 1295 1385 1916 2468 2989 3667 3941 4088
Size: 709 90 531 552 521 678 274 147
Cuts at: 4088 4499 586
Size: 411 1366
Fragments arranged by size:
1366 709 678 552 531 521 411 274
147 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1187 1217 1868 1898 2251 2281 1187
Size: 30 651 30 353 30 4185
Fragments arranged by size:
4185 651 353 30 30 30
BseYI C'CCAG_C
Cuts at: 1491 2704 3462 4039 4535 1491
Size: 1213 758 577 496 2235
Fragments arranged by size:
2235 1213 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2040 2040
Size: 5279
BsiEI CG_ry'CG
Cuts at: 104 2821 2867 4075 104
Size: 2717 46 1208 1308
Fragments arranged by size:
2717 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1130 1573 2293 3071 3261 4053 1130
Size: 443 720 778 190 792 2356
Fragments arranged by size:
2356 792 778 720 443 190
Bsp1286I G_dGCh'C
Cuts at: 139 288 393 503 730 974 1130 1573 1619
Size: 149 105 110 227 244 156 443 46
Cuts at: 1619 2019 2293 2904 2997 3071 3261 3323 4053
Size: 400 274 611 93 74 190 62 730
Cuts at: 4053 139
Size: 1365
Fragments arranged by size:
1365 730 611 443 400 274 244 227
190 156 149 110 105 93 74 62
46
BspEI T'CCGG_A
Cuts at: 586 1385 1916 4499 586
Size: 799 531 2583 1366
Fragments arranged by size:
2583 1366 799 531
BspHI T'CATG_A
Cuts at: 4455 4455
Size: 5279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2127 2329 2543 2845 3226 2127
Size: 202 214 302 381 4180
Fragments arranged by size:
4180 381 302 214 202
BsrBI CCG'CTC
Cuts at: 50 711 3571 3625 5109 5274 50
Size: 661 2860 54 1484 165 55
Fragments arranged by size:
2860 1484 661 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3191 3191
Size: 5279
BsrFI r'CCGG_y
Cuts at: 320 1818 3277 3458 3667 320
Size: 1498 1459 181 209 1932
Fragments arranged by size:
1932 1498 1459 209 181
BssSI C'ACGA_G
Cuts at: 124 271 3550 3908 124
Size: 147 3279 358 1495
Fragments arranged by size:
3279 1495 358 147
BstAPI GCAn_nnn'nTGC
Cuts at: 2656 2656
Size: 5279
BstBI TT'CG_AA
Cuts at: 872 3640 872
Size: 2768 2511
BstXI CCAn_nnnn'nTGG
Cuts at: 702 702
Size: 5279
BstYI r'GATC_y
Cuts at: 1 197 1310 1746 2049 2067 2304 2771 2794
Size: 196 1113 436 303 18 237 467 23
Cuts at: 2794 3129 3375 4387 4473 4485 4831 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1113 1012 467 449 436 346 335 303
246 237 196 86 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 202 1611 202
Size: 1409 3870
BtgI C'CryG_G
Cuts at: 474 695 932 1050 1191 1947 2270 474
Size: 221 237 118 141 756 323 3483
Fragments arranged by size:
3483 756 323 237 221 141 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1054 1793 3229 3410 3573 1054
Size: 739 1436 181 163 2760
Fragments arranged by size:
2760 1436 739 181 163
BtsI GCAGTG_nn'
Cuts at: 2522 2522
Size: 5279
DraI TTT'AAA
Cuts at: 1863 2278 1863
Size: 415 4864
DrdI GACnn_nn'nnGTC
Cuts at: 2985 3843 2985
Size: 858 4421
EaeI y'GGCC_r
Cuts at: 260 322 560 652 945 1557 2864 3038 3429
Size: 62 238 92 293 612 1307 174 391
Cuts at: 3429 3456 4801 260
Size: 27 1345 738
Fragments arranged by size:
1345 1307 738 612 391 293 238 174
92 62 27
EagI C'GGCC_G
Cuts at: 2864 2864
Size: 5279
EarI CTCTTCn'nnn_
Cuts at: 1432 2017 2246 3302 3512 1432
Size: 585 229 1056 210 3199
Fragments arranged by size:
3199 1056 585 229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 460 1216 3487 3797 3943 5089 460
Size: 756 2271 310 146 1146 650
Fragments arranged by size:
2271 1146 756 650 310 146
EcoICRI GAG'CTC
Cuts at: 2291 2291
Size: 5279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 580 821 1299 1372 1502 1817 2018 2092
Size: 384 241 478 73 130 315 201 74
Cuts at: 2092 3104 3536 4283 4551 196
Size: 1012 432 747 268 924
Fragments arranged by size:
1012 924 747 478 432 384 315 268
241 201 130 74 73
EcoNI CCTnn'n_nnAGG
Cuts at: 224 224
Size: 5279
EcoO109I rG'GnC_Cy
Cuts at: 500 727 1767 2418 500
Size: 227 1040 651 3361
Fragments arranged by size:
3361 1040 651 227
EcoRI G'AATT_C
Cuts at: 2283 2283
Size: 5279
EcoRV GAT'ATC
Cuts at: 1224 1224
Size: 5279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3298 3298
Size: 5279
FspI TGC'GCA
Cuts at: 3060 3060
Size: 5279
HaeII r_GCGC'y
Cuts at: 1344 1630 2713 2961 3983 4962 1344
Size: 286 1083 248 1022 979 1661
Fragments arranged by size:
1661 1083 1022 979 286 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 976 1008 1594 1626 2155 2187 3121 3151 3153
Size: 32 586 32 529 32 934 30 2
Cuts at: 3153 3183 976
Size: 30 3072
Fragments arranged by size:
3072 934 586 529 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2318 2318
Size: 5279
KasI G'GCGC_C
Cuts at: 1626 2709 2957 1626
Size: 1083 248 3948
Fragments arranged by size:
3948 1083 248
KpnI G_GTAC'C
Cuts at: 2299 2299
Size: 5279
MfeI C'AATT_G
Cuts at: 603 603
Size: 5279
MluI A'CGCG_T
Cuts at: 4712 4712
Size: 5279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3950 4134 3950
Size: 184 5095
MscI TGG'CCA
Cuts at: 947 1559 3040 947
Size: 612 1481 3186
Fragments arranged by size:
3186 1481 612
MslI CAynn'nnrTG
Cuts at: 271 437 700 859 1984 3268 3395 271
Size: 166 263 159 1125 1284 127 2155
Fragments arranged by size:
2155 1284 1125 263 166 159 127
MspA1I CmG'CkG
Cuts at: 760 1576 2390 2704 3064 4077 4322 760
Size: 816 814 314 360 1013 245 1717
Fragments arranged by size:
1717 1013 816 814 360 314 245
NaeI GCC'GGC
Cuts at: 1820 3460 1820
Size: 1640 3639
NarI GG'CG_CC
Cuts at: 1627 2710 2958 1627
Size: 1083 248 3948
Fragments arranged by size:
3948 1083 248
NcoI C'CATG_G
Cuts at: 695 1050 1947 2270 695
Size: 355 897 323 3704
Fragments arranged by size:
3704 897 355 323
NdeI CA'TA_TG
Cuts at: 2043 2043
Size: 5279
NgoMIV G'CCGG_C
Cuts at: 1818 3458 1818
Size: 1640 3639
NspI r_CATG'y
Cuts at: 617 820 1158 2169 2332 2832 3739 617
Size: 203 338 1011 163 500 907 2157
Fragments arranged by size:
2157 1011 907 500 338 203 163
PasI CC'CwG_GG
Cuts at: 723 730 723
Size: 7 5272
PciI A'CATG_T
Cuts at: 1154 2165 3735 1154
Size: 1011 1570 2698
Fragments arranged by size:
2698 1570 1011
PflMI CCAn_nnn'nTGG
Cuts at: 558 1929 558
Size: 1371 3908
PfoI T'CCnGG_A
Cuts at: 1349 1349
Size: 5279
PmeI GTTT'AAAC
Cuts at: 2278 2278
Size: 5279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 5247
PspOMI G'GGCC_C
Cuts at: 499 726 2015 499
Size: 227 1289 3763
Fragments arranged by size:
3763 1289 227
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 451 483 1285 1317 451
Size: 32 802 32 4413
Fragments arranged by size:
4413 802 32 32
PstI C_TGCA'G
Cuts at: 2326 3011 2326
Size: 685 4594
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5279
PvuII CAG'CTG
Cuts at: 1576 2704 3064 1576
Size: 1128 360 3791
Fragments arranged by size:
3791 1128 360
RsrII CG'GwC_CG
Cuts at: 3474 3474
Size: 5279
SacI G_AGCT'C
Cuts at: 2293 2293
Size: 5279
SalI G'TCGA_C
Cuts at: 2316 2316
Size: 5279
SapI GCTCTTCn'nnn_
Cuts at: 2246 3302 3512 2246
Size: 1056 210 4013
Fragments arranged by size:
4013 1056 210
SbfI CC_TGCA'GG
Cuts at: 2326 2326
Size: 5279
ScaI AGT'ACT
Cuts at: 2233 4796 2233
Size: 2563 2716
SexAI A'CCwGG_T
Cuts at: 461 461
Size: 5279
SfcI C'TryA_G
Cuts at: 33 2322 3007 4000 4191 33
Size: 2289 685 993 191 1121
Fragments arranged by size:
2289 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1628 2711 2959 1628
Size: 1083 248 3948
Fragments arranged by size:
3948 1083 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5279
SmaI CCC'GGG
Cuts at: 2301 2301
Size: 5279
SmlI C'TyrA_G
Cuts at: 844 2434 3349 3841 4103 844
Size: 1590 915 492 262 2020
Fragments arranged by size:
2020 1590 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5279
SphI G_CATG'C
Cuts at: 617 2332 2832 617
Size: 1715 500 3064
Fragments arranged by size:
3064 1715 500
StuI AGG'CCT
Cuts at: 331 842 1966 331
Size: 511 1124 3644
Fragments arranged by size:
3644 1124 511
StyI C'CwwG_G
Cuts at: 695 1050 1947 2270 2413 2767 695
Size: 355 897 323 143 354 3207
Fragments arranged by size:
3207 897 355 354 323 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 1063 1448 2862 2995 3454 3663 271
Size: 792 385 1414 133 459 209 1887
Fragments arranged by size:
1887 1414 792 459 385 209 133
TatI w'GTAC_w
Cuts at: 2231 4794 2231
Size: 2563 2716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 182 214 1709 1741 4796 4828 182
Size: 32 1495 32 3055 32 633
Fragments arranged by size:
3055 1495 633 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3076 3076
Size: 5279
XbaI T'CTAG_A
Cuts at: 2310 2310
Size: 5279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 930 1272 1340 2774 930
Size: 342 68 1434 3435
Fragments arranged by size:
3435 1434 342 68
XmaI C'CCGG_G
Cuts at: 2299 2299
Size: 5279
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI
FspAI HindIII HpaI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI