Restriction map for
pF1KA0834
(Circular) (Six-base) MAPSORT of: pF1KA0834.seq Check: 6318 from: 1 to: 4466
pF1KA0834 4466 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 994 994
Size: 4466
AccI GT'mk_AC
Cuts at: 196 500 1504 196
Size: 304 1004 3158
Fragments arranged by size:
3158 1004 304
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 2291 2723 3470 3738 562
Size: 1729 432 747 268 1290
Fragments arranged by size:
1729 1290 747 432 268
AflIII A'CryG_T
Cuts at: 995 1346 1818 2922 3899 995
Size: 351 472 1104 977 1562
Fragments arranged by size:
1562 1104 977 472 351
AgeI A'CCGG_T
Cuts at: 2854 2854
Size: 4466
AlwNI CAG_nnn'CTG
Cuts at: 774 1393 1771 3338 774
Size: 619 378 1567 1902
Fragments arranged by size:
1902 1567 619 378
ApaLI G'TGCA_C
Cuts at: 691 3236 691
Size: 2545 1921
ApoI r'AATT_y
Cuts at: 436 1470 3819 4075 436
Size: 1034 2349 256 827
Fragments arranged by size:
2349 1034 827 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 1486 1486
Size: 4466
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 1482 1896 2144 2179 1482
Size: 414 248 35 3769
Fragments arranged by size:
3769 414 248 35
BanII G_rGCy'C
Cuts at: 146 1480 2510 146
Size: 1334 1030 2102
Fragments arranged by size:
2102 1334 1030
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 1343 1343
Size: 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4432
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3125 4466 2364
Size: 761 1341 2364
Fragments arranged by size:
2364 1341 761
BclI T'GATC_A
Cuts at: 227 548 842 1986 227
Size: 321 294 1144 2707
Fragments arranged by size:
2707 1144 321 294
BglI GCCn_nnn'nGGC
Cuts at: 1326 1326
Size: 4466
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 1210 1578 1210
Size: 368 4098
Bme1580I G_kGCm'C
Cuts at: 695 2091 2184 3240 695
Size: 1396 93 1056 1921
Fragments arranged by size:
1921 1396 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1678 1759 2094 4238 1678
Size: 81 335 2144 1906
Fragments arranged by size:
2144 1906 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 935 1148 1415 393
Size: 542 213 267 3444
Fragments arranged by size:
3444 542 267 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1314 1642 2521 3013 3311 1314
Size: 328 879 492 298 2469
Fragments arranged by size:
2469 879 492 328 298
BsaI GGTCTCn'nnnn_
Cuts at: 812 4170 812
Size: 3358 1108
BsaAI yAC'GTr
Cuts at: 1819 1819
Size: 4466
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 1897 2145 2847 4343 1897
Size: 248 702 1496 2020
Fragments arranged by size:
2020 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3128 3275 3686 1655
Size: 521 678 274 147 411 2435
Fragments arranged by size:
2435 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 901 931 1225 1255 901
Size: 30 294 30 4112
Fragments arranged by size:
4112 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 360 360
Size: 4466
BseYI C'CCAG_C
Cuts at: 415 1891 2649 3226 3722 415
Size: 1476 758 577 496 1159
Fragments arranged by size:
1476 1159 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3262 104
Size: 1904 46 1208 1308
Fragments arranged by size:
1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 289 695 1480 2258 2448 3240 289
Size: 406 785 778 190 792 1515
Fragments arranged by size:
1515 792 785 778 406 190
BsmI GAATG_Cn'
Cuts at: 333 333
Size: 4466
BsmBI CGTCTCn'nnnn_
Cuts at: 658 658
Size: 4466
Bsp1286I G_dGCh'C
Cuts at: 146 289 695 1480 2091 2184 2258 2448 2510
Size: 143 406 785 611 93 74 190 62
Cuts at: 2510 3240 146
Size: 730 1372
Fragments arranged by size:
1372 785 730 611 406 190 143 93
74 62
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 642 3642 642
Size: 3000 1466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 994 1325 1516 1730 2032 2413 994
Size: 331 191 214 302 381 3047
Fragments arranged by size:
3047 381 331 302 214 191
BsrBI CCG'CTC
Cuts at: 50 2758 2812 4296 4461 50
Size: 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 135 2378 135
Size: 2243 2223
BsrFI r'CCGG_y
Cuts at: 2464 2645 2854 2464
Size: 181 209 4076
Fragments arranged by size:
4076 209 181
BsrGI T'GTAC_A
Cuts at: 1172 1172
Size: 4466
BssSI C'ACGA_G
Cuts at: 1300 2737 3095 1300
Size: 1437 358 2671
Fragments arranged by size:
2671 1437 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1288 1843 1288
Size: 555 3911
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstEII G'GTnAC_C
Cuts at: 930 930
Size: 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 927 1331 927
Size: 404 4062
BstYI r'GATC_y
Cuts at: 1 484 1491 1958 1981 2316 2562 3574 3660
Size: 483 1007 467 23 335 246 1012 86
Cuts at: 3660 3672 4018 1
Size: 12 346 449
Fragments arranged by size:
1012 1007 483 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 501 501
Size: 4466
Bsu36I CC'TnA_GG
Cuts at: 251 251
Size: 4466
BtgI C'CryG_G
Cuts at: 1324 1324
Size: 4466
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 1709 1709
Size: 4466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 972 1007 972
Size: 35 4431
DraI TTT'AAA
Cuts at: 608 1465 608
Size: 857 3609
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3030 2172
Size: 858 3608
EaeI y'GGCC_r
Cuts at: 2051 2225 2616 2643 3988 2051
Size: 174 391 27 1345 2529
Fragments arranged by size:
2529 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 2984 3130 4276 2674
Size: 310 146 1146 2864
Fragments arranged by size:
2864 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1478 1478
Size: 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 562 935 1148 1415 2291 2723 3470 3738
Size: 169 373 213 267 876 432 747 268
Cuts at: 3738 393
Size: 1121
Fragments arranged by size:
1121 876 747 432 373 268 267 213
169
EcoO109I rG'GnC_Cy
Cuts at: 1233 1605 1233
Size: 372 4094
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 619 2485 619
Size: 1866 2600
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 1900 2148 3170 4149 1900
Size: 248 1022 979 2217
Fragments arranged by size:
2217 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1225 1257 2308 2338 2340 2370 1225
Size: 32 1051 30 2 30 3321
Fragments arranged by size:
3321 1051 32 30 30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
HindIII A'AGCT_T
Cuts at: 596 596
Size: 4466
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 944 3137 3321 944
Size: 2193 184 2089
Fragments arranged by size:
2193 2089 184
MscI TGG'CCA
Cuts at: 2227 2227
Size: 4466
MslI CAynn'nnrTG
Cuts at: 177 190 1329 1345 2455 2582 177
Size: 13 1139 16 1110 127 2061
Fragments arranged by size:
2061 1139 1110 127 16 13
MspA1I CmG'CkG
Cuts at: 1577 1891 2251 3264 3509 1577
Size: 314 360 1013 245 2534
Fragments arranged by size:
2534 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NdeI CA'TA_TG
Cuts at: 456 456
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NspI r_CATG'y
Cuts at: 625 999 1350 1407 1519 2019 2926 625
Size: 374 351 57 112 500 907 2165
Fragments arranged by size:
2165 907 500 374 351 112 57
PasI CC'CwG_GG
Cuts at: 727 727
Size: 4466
PciI A'CATG_T
Cuts at: 995 1346 2922 995
Size: 351 1576 2539
Fragments arranged by size:
2539 1576 351
PflMI CCAn_nnn'nTGG
Cuts at: 216 1393 216
Size: 1177 3289
PfoI T'CCnGG_A
Cuts at: 1050 1050
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4434
PpuMI rG'GwC_Cy
Cuts at: 1233 1233
Size: 4466
PstI C_TGCA'G
Cuts at: 141 560 1513 2198 141
Size: 419 953 685 2409
Fragments arranged by size:
2409 953 685 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 1891 2251 1891
Size: 360 4106
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1480 1480
Size: 4466
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 137 494 556 1509 2194 3187 3378 33
Size: 104 357 62 953 685 993 191 1121
Fragments arranged by size:
1121 993 953 685 357 191 104 62
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 1488 1488
Size: 4466
SmlI C'TyrA_G
Cuts at: 1293 1621 2536 3028 3290 1293
Size: 328 915 492 262 2469
Fragments arranged by size:
2469 915 492 328 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1407 1519 2019 1407
Size: 112 500 3854
Fragments arranged by size:
3854 500 112
SspI AAT'ATT
Cuts at: 979 1088 979
Size: 109 4357
StyI C'CwwG_G
Cuts at: 659 1032 1600 1954 659
Size: 373 568 354 3171
Fragments arranged by size:
3171 568 373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 868 2049 2182 2641 2850 868
Size: 1181 133 459 209 2484
Fragments arranged by size:
2484 1181 459 209 133
TatI w'GTAC_w
Cuts at: 710 1172 3981 710
Size: 462 2809 1195
Fragments arranged by size:
2809 1195 462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 901 933 3983 4015 901
Size: 32 3050 32 1352
Fragments arranged by size:
3050 1352 32 32
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1961 1961
Size: 4466
XmaI C'CCGG_G
Cuts at: 1486 1486
Size: 4466
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BssHII ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI