Restriction map for
pF1KA0816
(Circular) (Six-base) MAPSORT of: pF1KA0816.seq Check: 151 from: 1 to: 8819
pF1KA0816 8819 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1452 1452
Size: 8819
AccI GT'mk_AC
Cuts at: 306 1627 3115 3371 3521 5857 306
Size: 1321 1488 256 150 2336 3268
Fragments arranged by size:
3268 2336 1488 1321 256 150
Acc65I G'GTAC_C
Cuts at: 1804 3327 4547 5061 5835 1804
Size: 1523 1220 514 774 4788
Fragments arranged by size:
4788 1523 1220 774 514
AclI AA'CG_TT
Cuts at: 8659 8659
Size: 8819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 4408 6644 7076 7823 8091 4150
Size: 258 2236 432 747 268 4878
Fragments arranged by size:
4878 2236 747 432 268 258
AfeI AGC'GCT
Cuts at: 2103 3018 2103
Size: 915 7904
AflIII A'CryG_T
Cuts at: 205 1280 2465 2723 6171 7275 8252 205
Size: 1075 1185 258 3448 1104 977 772
Fragments arranged by size:
3448 1185 1104 1075 977 772 258
AgeI A'CCGG_T
Cuts at: 3858 7207 3858
Size: 3349 5470
AhdI GACnn_n'nnGTC
Cuts at: 5640 5640
Size: 8819
AleI CACnn'nnGTG
Cuts at: 2312 4234 4315 2312
Size: 1922 81 6816
Fragments arranged by size:
6816 1922 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3229 3229
Size: 8819
AlwNI CAG_nnn'CTG
Cuts at: 1059 1426 1467 1764 6124 7691 1059
Size: 367 41 297 4360 1567 2187
Fragments arranged by size:
4360 2187 1567 367 297 41
ApaI G_GGCC'C
Cuts at: 1737 1737
Size: 8819
ApaLI G'TGCA_C
Cuts at: 5219 7589 5219
Size: 2370 6449
ApoI r'AATT_y
Cuts at: 2110 2890 5823 8172 8428 2110
Size: 780 2933 2349 256 2501
Fragments arranged by size:
2933 2501 2349 780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8819
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8819
AvaI C'yCGr_G
Cuts at: 175 428 1704 1775 2453 2690 3614 4050 5839
Size: 253 1276 71 678 237 924 436 1789
Cuts at: 5839 175
Size: 3155
Fragments arranged by size:
3155 1789 1276 924 678 436 253 237
71
AvrII C'CTAG_G
Cuts at: 3735 5074 3735
Size: 1339 7480
BamHI G'GATC_C
Cuts at: 1580 5844 1580
Size: 4264 4555
BanI G'GyrC_C
Cuts at: 139 1699 1804 1934 3327 3832 4547 5061 5835
Size: 1560 105 130 1393 505 715 514 774
Cuts at: 5835 6249 6497 6532 139
Size: 414 248 35 2426
Fragments arranged by size:
2426 1560 1393 774 715 514 505 414
248 130 105 35
BanII G_rGCy'C
Cuts at: 1645 1737 2560 2752 3494 4259 5104 5833 6863
Size: 92 823 192 742 765 845 729 1030
Cuts at: 6863 1645
Size: 3601
Fragments arranged by size:
3601 1030 845 823 765 742 729 192
92
BbeI G_GCGC'C
Cuts at: 143 1938 6253 6501 143
Size: 1795 4315 248 2461
Fragments arranged by size:
4315 2461 1795 248
BbsI GAAGACnn'nnnn_
Cuts at: 1545 2063 2887 3081 4150 4396 5635 1545
Size: 518 824 194 1069 246 1239 4729
Fragments arranged by size:
4729 1239 1069 824 518 246 194
BbvCI CC'TCA_GC
Cuts at: 657 4922 657
Size: 4265 4554
BciVI GTATCCnnnnn_n'
Cuts at: 2535 6717 7478 8819 2535
Size: 4182 761 1341 2535
Fragments arranged by size:
4182 2535 1341 761
BclI T'GATC_A
Cuts at: 4502 6339 4502
Size: 1837 6982
BfrBI ATG'CAT
Cuts at: 5624 5624
Size: 8819
BglI GCCn_nnn'nGGC
Cuts at: 464 1253 1395 1649 2285 2564 3203 4118 464
Size: 789 142 254 636 279 639 915 5165
Fragments arranged by size:
5165 915 789 639 636 279 254 142
BglII A'GATC_T
Cuts at: 6334 8371 6334
Size: 2037 6782
BlpI GC'TnA_GC
Cuts at: 593 1958 5931 593
Size: 1365 3973 3481
Fragments arranged by size:
3973 3481 1365
Bme1580I G_kGCm'C
Cuts at: 1206 1280 1737 2319 4023 5223 6444 6537 7593
Size: 74 457 582 1704 1200 1221 93 1056
Cuts at: 7593 1206
Size: 2432
Fragments arranged by size:
2432 1704 1221 1200 1056 582 457 93
74
BmgBI CAC'GTC
Cuts at: 2421 5162 2421
Size: 2741 6078
BmrI ACTGGGnnnn_n'
Cuts at: 243 1640 1833 1956 2246 2454 2748 2871 3103
Size: 1397 193 123 290 208 294 123 232
Cuts at: 3103 3672 4425 4575 4698 5098 6031 6112 6447
Size: 569 753 150 123 400 933 81 335
Cuts at: 6447 8591 243
Size: 2144 471
Fragments arranged by size:
2144 1397 933 753 569 471 400 335
294 290 232 208 193 150 123 123
123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 420 7186 420
Size: 6766 2053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1528 1657 1786 1836 4167 5188 5234 5726
Size: 822 129 129 50 2331 1021 46 492
Cuts at: 5726 706
Size: 3799
Fragments arranged by size:
3799 2331 1021 822 492 129 129 50
46
Bpu10I CC'TnA_GC
Cuts at: 657 4253 4922 657
Size: 3596 669 4554
Fragments arranged by size:
4554 3596 669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5560 5995 6874 7366 7664 5560
Size: 435 879 492 298 6715
Fragments arranged by size:
6715 879 492 435 298
BsaI GGTCTCn'nnnn_
Cuts at: 3297 4159 8523 3297
Size: 862 4364 3593
Fragments arranged by size:
4364 3593 862
BsaAI yAC'GTr
Cuts at: 1283 2466 6172 1283
Size: 1183 3706 3930
Fragments arranged by size:
3930 3706 1183
BsaBI GATnn'nnATC
Cuts at: 6 1892 2359 4700 5521 6333 8370 6
Size: 1886 467 2341 821 812 2037 455
Fragments arranged by size:
2341 2037 1886 821 812 467 455
BsaHI Gr'CG_yC
Cuts at: 140 1872 1935 2870 4468 6250 6498 7200 8696
Size: 1732 63 935 1598 1782 248 702 1496
Cuts at: 8696 140
Size: 263
Fragments arranged by size:
1782 1732 1598 1496 935 702 263 248
63
BsaWI w'CCGG_w
Cuts at: 476 1577 1739 2621 3545 3691 3858 4709 6008
Size: 1101 162 882 924 146 167 851 1299
Cuts at: 6008 6529 7207 7481 7628 8039 476
Size: 521 678 274 147 411 1256
Fragments arranged by size:
1299 1256 1101 924 882 851 678 521
411 274 167 162 147 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 682 712 769 799 802 832 1609
Size: 30 219 30 57 30 3 30 777
Cuts at: 1609 1639 1986 2016 2434 2464 5677 5707 433
Size: 30 347 30 418 30 3213 30 3545
Fragments arranged by size:
3545 3213 777 418 347 219 57 30
30 30 30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 658 703 774 1186 1324 1630 5185 5281
Size: 204 45 71 412 138 306 3555 96
Cuts at: 5281 5596 5787 454
Size: 315 191 3486
Fragments arranged by size:
3555 3486 412 315 306 204 191 138
96 71 45
BseYI C'CCAG_C
Cuts at: 2183 2559 2849 5208 6244 7002 7579 8075 2183
Size: 376 290 2359 1036 758 577 496 2927
Fragments arranged by size:
2927 2359 1036 758 577 496 376 290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 885 1013 1255 1317 2217 3397 3883 4953
Size: 655 128 242 62 900 1180 486 1070
Cuts at: 4953 230
Size: 4096
Fragments arranged by size:
4096 1180 1070 900 655 486 242 128
62
BsiEI CG_ry'CG
Cuts at: 104 1395 6361 6407 7615 104
Size: 1291 4966 46 1208 1308
Fragments arranged by size:
4966 1308 1291 1208 46
BsiHKAI G_wGCw'C
Cuts at: 222 553 3722 4259 5223 5833 6611 6801 7593
Size: 331 3169 537 964 610 778 190 792
Cuts at: 7593 222
Size: 1448
Fragments arranged by size:
3169 1448 964 792 778 610 537 331
190
BsmI GAATG_Cn'
Cuts at: 1518 3678 1518
Size: 2160 6659
BsmBI CGTCTCn'nnnn_
Cuts at: 5168 5674 5168
Size: 506 8313
Bsp1286I G_dGCh'C
Cuts at: 222 553 1206 1280 1645 1737 2319 2560 2752
Size: 331 653 74 365 92 582 241 192
Cuts at: 2752 3494 3722 4023 4259 5104 5223 5833 6444
Size: 742 228 301 236 845 119 610 611
Cuts at: 6444 6537 6611 6801 6863 7593 222
Size: 93 74 190 62 730 1448
Fragments arranged by size:
1448 845 742 730 653 611 610 582
365 331 301 241 236 228 192 190
119 93 92 74 74 62
BspEI T'CCGG_A
Cuts at: 476 3691 8039 476
Size: 3215 4348 1256
Fragments arranged by size:
4348 3215 1256
BspHI T'CATG_A
Cuts at: 7995 7995
Size: 8819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 1252 1452 2202 2602 3202 3221 3241 3992
Size: 1008 200 750 400 600 19 20 751
Cuts at: 3992 4181 4737 5869 6083 6385 6766 244
Size: 189 556 1132 214 302 381 2297
Fragments arranged by size:
2297 1132 1008 751 750 600 556 400
381 302 214 200 189 20 19
BsrBI CCG'CTC
Cuts at: 50 365 803 1478 3687 4614 7111 7165 8649
Size: 315 438 675 2209 927 2497 54 1484
Cuts at: 8649 8814 50
Size: 165 55
Fragments arranged by size:
2497 2209 1484 927 675 438 315 165
55 54
BsrDI GCAATG_nn'
Cuts at: 587 1188 1517 1781 1880 3356 3966 4523 4574
Size: 601 329 264 99 1476 610 557 51
Cuts at: 4574 6731 587
Size: 2157 2675
Fragments arranged by size:
2675 2157 1476 610 601 557 329 264
99 51
BsrFI r'CCGG_y
Cuts at: 1154 1259 1394 2872 3023 3101 3858 6817 6998
Size: 105 135 1478 151 78 757 2959 181
Cuts at: 6998 7207 1154
Size: 209 2766
Fragments arranged by size:
2959 2766 1478 757 209 181 151 135
105 78
BsrGI T'GTAC_A
Cuts at: 5336 5456 5336
Size: 120 8699
BssHII G'CGCG_C
Cuts at: 125 125
Size: 8819
BssSI C'ACGA_G
Cuts at: 3970 7090 7448 3970
Size: 3120 358 5341
Fragments arranged by size:
5341 3120 358
BstAPI GCAn_nnn'nTGC
Cuts at: 217 1467 3233 6196 217
Size: 1250 1766 2963 2840
Fragments arranged by size:
2963 2840 1766 1250
BstBI TT'CG_AA
Cuts at: 7180 7180
Size: 8819
BstEII G'GTnAC_C
Cuts at: 2583 2583
Size: 8819
BstXI CCAn_nnnn'nTGG
Cuts at: 739 2953 739
Size: 2214 6605
BstYI r'GATC_y
Cuts at: 1 1580 4682 4802 5844 6311 6334 6669 6915
Size: 1579 3102 120 1042 467 23 335 246
Cuts at: 6915 7927 8013 8025 8371 1
Size: 1012 86 12 346 449
Fragments arranged by size:
3102 1579 1042 1012 467 449 346 335
246 120 86 23 12
BstZ17I GTA'TAC
Cuts at: 307 3522 307
Size: 3215 5604
Bsu36I CC'TnA_GG
Cuts at: 2781 2781
Size: 8819
BtgI C'CryG_G
Cuts at: 1796 2711 4188 1796
Size: 915 1477 6427
Fragments arranged by size:
6427 1477 915
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 305 509 539 752 872 1019 6769 6950 7113
Size: 204 30 213 120 147 5750 181 163
Cuts at: 7113 305
Size: 2011
Fragments arranged by size:
5750 2011 213 204 181 163 147 120
30
BtsI GCAGTG_nn'
Cuts at: 124 218 262 489 538 668 732 1238 1243
Size: 94 44 227 49 130 64 506 5
Cuts at: 1243 1489 2433 3409 4415 6062 124
Size: 246 944 976 1006 1647 2881
Fragments arranged by size:
2881 1647 1006 976 944 506 246 227
130 94 64 49 44 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 492 527 1098 1133 4780 4815 4929 4964 492
Size: 35 571 35 3647 35 114 35 4347
Fragments arranged by size:
4347 3647 571 114 35 35 35 35
DraI TTT'AAA
Cuts at: 5818 5818
Size: 8819
DraIII CAC_nnn'GTG
Cuts at: 3124 3124
Size: 8819
DrdI GACnn_nn'nnGTC
Cuts at: 3085 5160 6525 7383 3085
Size: 2075 1365 858 4521
Fragments arranged by size:
4521 2075 1365 858
EaeI y'GGCC_r
Cuts at: 164 1718 2256 2570 3510 4421 4758 6404 6578
Size: 1554 538 314 940 911 337 1646 174
Cuts at: 6578 6969 6996 8341 164
Size: 391 27 1345 642
Fragments arranged by size:
1646 1554 1345 940 911 642 538 391
337 314 174 27
EagI C'GGCC_G
Cuts at: 6404 6404
Size: 8819
EarI CTCTTCn'nnn_
Cuts at: 1004 1903 2808 4183 4558 5696 6842 7052 1004
Size: 899 905 1375 375 1138 1146 210 2771
Fragments arranged by size:
2771 1375 1146 1138 905 899 375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 349 946 7027 7337 7483 8629 349
Size: 597 6081 310 146 1146 539
Fragments arranged by size:
6081 1146 597 539 310 146
EcoICRI GAG'CTC
Cuts at: 4257 5831 4257
Size: 1574 7245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1528 1657 1786 1836 4150 4167 4408 5188
Size: 822 129 129 50 2314 17 241 780
Cuts at: 5188 5234 5726 6644 7076 7823 8091 706
Size: 46 492 918 432 747 268 1434
Fragments arranged by size:
2314 1434 918 822 780 747 492 432
268 241 129 129 50 46 17
EcoO109I rG'GnC_Cy
Cuts at: 1650 1733 2022 2388 2846 3164 4620 5488 5601
Size: 83 289 366 458 318 1456 868 113
Cuts at: 5601 5958 1650
Size: 357 4511
Fragments arranged by size:
4511 1456 868 458 366 357 318 289
113 83
EcoRI G'AATT_C
Cuts at: 5823 5823
Size: 8819
EcoRV GAT'ATC
Cuts at: 4905 4905
Size: 8819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6838 6838
Size: 8819
FspI TGC'GCA
Cuts at: 153 544 2425 4700 6600 153
Size: 391 1881 2275 1900 2372
Fragments arranged by size:
2372 2275 1900 1881 391
FspAI rTGC'GCAy
Cuts at: 4700 4700
Size: 8819
HaeII r_GCGC'y
Cuts at: 143 673 855 972 1077 1938 2105 2190 3020
Size: 530 182 117 105 861 167 85 830
Cuts at: 3020 6253 6501 7523 8502 143
Size: 3233 248 1022 979 460
Fragments arranged by size:
3233 1022 979 861 830 530 460 248
182 167 117 105 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 701 733 769 801 868 900 1609 1641 1862
Size: 32 36 32 67 32 709 32 221
Cuts at: 1862 1894 1968 2000 2377 2409 2434 2466 2830
Size: 32 74 32 377 32 25 32 364
Cuts at: 2830 2862 2963 2995 4137 4169 4837 4869 5252
Size: 32 101 32 1142 32 668 32 383
Cuts at: 5252 5284 5626 5658 6661 6691 6693 6723 701
Size: 32 342 32 1003 30 2 30 2797
Fragments arranged by size:
2797 1142 1003 709 668 383 377 364
342 221 101 74 67 36 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 1110 1188 4845 5858 1110
Size: 78 3657 1013 4071
Fragments arranged by size:
4071 3657 1013 78
HpaI GTT'AAC
Cuts at: 1188 1188
Size: 8819
KasI G'GCGC_C
Cuts at: 139 1934 6249 6497 139
Size: 1795 4315 248 2461
Fragments arranged by size:
4315 2461 1795 248
KpnI G_GTAC'C
Cuts at: 1808 3331 4551 5065 5839 1808
Size: 1523 1220 514 774 4788
Fragments arranged by size:
4788 1523 1220 774 514
MfeI C'AATT_G
Cuts at: 2885 2885
Size: 8819
MluI A'CGCG_T
Cuts at: 8252 8252
Size: 8819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 172 578 1045 3345 4004 7490 7674 172
Size: 406 467 2300 659 3486 184 1317
Fragments arranged by size:
3486 2300 1317 659 467 406 184
MscI TGG'CCA
Cuts at: 166 1720 2258 2572 4423 6580 166
Size: 1554 538 314 1851 2157 2405
Fragments arranged by size:
2405 2157 1851 1554 538 314
MslI CAynn'nnrTG
Cuts at: 290 494 737 910 2047 2312 2701 3286 3343
Size: 204 243 173 1137 265 389 585 57
Cuts at: 3343 3872 3970 4000 4232 4234 4315 6808 6935
Size: 529 98 30 232 2 81 2493 127
Cuts at: 6935 290
Size: 2174
Fragments arranged by size:
2493 2174 1137 585 529 389 265 243
232 204 173 127 98 81 57 30
2
MspA1I CmG'CkG
Cuts at: 569 935 950 1403 2210 2563 3188 4128 4926
Size: 366 15 453 807 353 625 940 798
Cuts at: 4926 5466 5691 5930 6244 6604 7617 7862 569
Size: 540 225 239 314 360 1013 245 1526
Fragments arranged by size:
1526 1013 940 807 798 625 540 453
366 360 353 314 245 239 225 15
NaeI GCC'GGC
Cuts at: 1156 2874 3103 7000 1156
Size: 1718 229 3897 2975
Fragments arranged by size:
3897 2975 1718 229
NarI GG'CG_CC
Cuts at: 140 1935 6250 6498 140
Size: 1795 4315 248 2461
Fragments arranged by size:
4315 2461 1795 248
NcoI C'CATG_G
Cuts at: 1796 2711 1796
Size: 915 7904
NdeI CA'TA_TG
Cuts at: 3873 3873
Size: 8819
NgoMIV G'CCGG_C
Cuts at: 1154 2872 3101 6998 1154
Size: 1718 229 3897 2975
Fragments arranged by size:
3897 2975 1718 229
NsiI A_TGCA'T
Cuts at: 5626 5626
Size: 8819
NspI r_CATG'y
Cuts at: 209 543 2727 4005 4243 5872 6372 7279 209
Size: 334 2184 1278 238 1629 500 907 1749
Fragments arranged by size:
2184 1749 1629 1278 907 500 334 238
PasI CC'CwG_GG
Cuts at: 1645 1645
Size: 8819
PciI A'CATG_T
Cuts at: 205 2723 7275 205
Size: 2518 4552 1749
Fragments arranged by size:
4552 2518 1749
PflMI CCAn_nnn'nTGG
Cuts at: 1814 2579 3639 1814
Size: 765 1060 6994
Fragments arranged by size:
6994 1060 765
PfoI T'CCnGG_A
Cuts at: 4322 5367 5609 4322
Size: 1045 242 7532
Fragments arranged by size:
7532 1045 242
PmeI GTTT'AAAC
Cuts at: 5818 5818
Size: 8819
PmlI CAC'GTG
Cuts at: 1283 2466 1283
Size: 1183 7636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 672 5777 5809 7958 7990 640
Size: 32 5105 32 2149 32 1469
Fragments arranged by size:
5105 2149 1469 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2388 2846 3164 4620 2388
Size: 458 318 1456 6587
Fragments arranged by size:
6587 1456 458 318
PshAI GACnn'nnGTC
Cuts at: 2363 2479 4448 2363
Size: 116 1969 6734
Fragments arranged by size:
6734 1969 116
PspOMI G'GGCC_C
Cuts at: 1733 1733
Size: 8819
PspXI vC'TCGA_Gb
Cuts at: 1704 1704
Size: 8819
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5371 5403 5371
Size: 32 8787
PstI C_TGCA'G
Cuts at: 2199 3056 5736 5866 6551 2199
Size: 857 2680 130 685 4467
Fragments arranged by size:
4467 2680 857 685 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8819
PvuII CAG'CTG
Cuts at: 1403 2563 3188 4128 5466 5691 6244 6604 1403
Size: 1160 625 940 1338 225 553 360 3618
Fragments arranged by size:
3618 1338 1160 940 625 553 360 225
RsrII CG'GwC_CG
Cuts at: 3689 7014 3689
Size: 3325 5494
SacI G_AGCT'C
Cuts at: 4259 5833 4259
Size: 1574 7245
SalI G'TCGA_C
Cuts at: 5856 5856
Size: 8819
SanDI GG'GwC_CC
Cuts at: 2846 2846
Size: 8819
SapI GCTCTTCn'nnn_
Cuts at: 4183 5696 6842 7052 4183
Size: 1513 1146 210 5950
Fragments arranged by size:
5950 1513 1146 210
SbfI CC_TGCA'GG
Cuts at: 2199 5866 2199
Size: 3667 5152
ScaI AGT'ACT
Cuts at: 2663 4499 8336 2663
Size: 1836 3837 3146
Fragments arranged by size:
3837 3146 1836
SfcI C'TryA_G
Cuts at: 33 570 2195 3052 3144 3475 5388 5732 5862
Size: 537 1625 857 92 331 1913 344 130
Cuts at: 5862 6547 7540 7731 33
Size: 685 993 191 1121
Fragments arranged by size:
1913 1625 1121 993 857 685 537 344
331 191 130 92
SfoI GGC'GCC
Cuts at: 141 1936 6251 6499 141
Size: 1795 4315 248 2461
Fragments arranged by size:
4315 2461 1795 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8819
SgrAI Cr'CCGG_yG
Cuts at: 3858 3858
Size: 8819
SmaI CCC'GGG
Cuts at: 430 1777 2455 3616 5841 430
Size: 1347 678 1161 2225 3408
Fragments arranged by size:
3408 2225 1347 1161 678
SmlI C'TyrA_G
Cuts at: 1704 4050 5575 5974 6889 7381 7643 1704
Size: 2346 1525 399 915 492 262 2880
Fragments arranged by size:
2880 2346 1525 915 492 399 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8819
SphI G_CATG'C
Cuts at: 5872 6372 5872
Size: 500 8319
StuI AGG'CCT
Cuts at: 1849 3992 1849
Size: 2143 6676
StyI C'CwwG_G
Cuts at: 1046 1796 2711 3735 5074 5507 5953 6307 1046
Size: 750 915 1024 1339 433 446 354 3558
Fragments arranged by size:
3558 1339 1024 915 750 446 433 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 360 1705 1938 2963 3884 4407 4680 4987 6402
Size: 1345 233 1025 921 523 273 307 1415
Cuts at: 6402 6535 6994 7203 360
Size: 133 459 209 1976
Fragments arranged by size:
1976 1415 1345 1025 921 523 459 307
273 233 209 133
TatI w'GTAC_w
Cuts at: 1484 1943 2066 2468 2597 2661 3392 3650 4433
Size: 459 123 402 129 64 731 258 783
Cuts at: 4433 4497 5336 5456 8334 1484
Size: 64 839 120 2878 1969
Fragments arranged by size:
2878 1969 839 783 731 459 402 258
129 123 120 64 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 802 834 1572 1604 2516 2548 3411
Size: 32 88 32 738 32 912 32 863
Cuts at: 3411 3443 3743 3775 4134 4166 4704 4736 5335
Size: 32 300 32 359 32 538 32 599
Cuts at: 5335 5367 8336 8368 682
Size: 32 2969 32 1133
Fragments arranged by size:
2969 1133 912 863 738 599 538 359
300 88 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4827 6616 4827
Size: 1789 7030
XbaI T'CTAG_A
Cuts at: 5850 5850
Size: 8819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 703 4401 5656 5771 6314 703
Size: 3698 1255 115 543 3208
Fragments arranged by size:
3698 3208 1255 543 115
XhoI C'TCGA_G
Cuts at: 1704 4050 1704
Size: 2346 6473
XmaI C'CCGG_G
Cuts at: 428 1775 2453 3614 5839 428
Size: 1347 678 1161 2225 3408
Fragments arranged by size:
3408 2225 1347 1161 678
XmnI GAAnn'nnTTC
Cuts at: 2135 3253 5261 2135
Size: 1118 2008 5693
Fragments arranged by size:
5693 2008 1118
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI AscI BaeI BcgI BmtI BsiWI
ClaI EcoNI FseI HindIII NheI NotI NruI PacI
PsiI SacII SexAI SfiI SnaBI SrfI SspI SwaI
ZraI