Restriction map for
pF1KA0808
(Circular) (Six-base) MAPSORT of: pF1KA0808.seq Check: 1929 from: 1 to: 4691
pF1KA0808 4691 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1729 1729
Size: 4691
Acc65I G'GTAC_C
Cuts at: 1707 1707
Size: 4691
AclI AA'CG_TT
Cuts at: 4531 4531
Size: 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 2516 2948 3695 3963 1093
Size: 1423 432 747 268 1821
Fragments arranged by size:
1821 1423 747 432 268
AflIII A'CryG_T
Cuts at: 224 2043 3147 4124 224
Size: 1819 1104 977 791
Fragments arranged by size:
1819 1104 977 791
AgeI A'CCGG_T
Cuts at: 1617 3079 1617
Size: 1462 3229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 689 721 689
Size: 32 4659
AlwNI CAG_nnn'CTG
Cuts at: 1327 1996 3563 1327
Size: 669 1567 2455
Fragments arranged by size:
2455 1567 669
ApaI G_GGCC'C
Cuts at: 1193 1193
Size: 4691
ApaLI G'TGCA_C
Cuts at: 358 3461 358
Size: 3103 1588
ApoI r'AATT_y
Cuts at: 1695 4044 4300 1695
Size: 2349 256 2086
Fragments arranged by size:
2349 2086 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4691
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
AvaI C'yCGr_G
Cuts at: 1711 1711
Size: 4691
BamHI G'GATC_C
Cuts at: 876 1716 876
Size: 840 3851
BanI G'GyrC_C
Cuts at: 1707 2121 2369 2404 1707
Size: 414 248 35 3994
Fragments arranged by size:
3994 414 248 35
BanII G_rGCy'C
Cuts at: 890 1193 1705 2735 890
Size: 303 512 1030 2846
Fragments arranged by size:
2846 1030 512 303
BbeI G_GCGC'C
Cuts at: 2125 2373 2125
Size: 248 4443
BbsI GAAGACnn'nnnn_
Cuts at: 311 1144 311
Size: 833 3858
BbvCI CC'TCA_GC
Cuts at: 1154 1154
Size: 4691
BciVI GTATCCnnnnn_n'
Cuts at: 1116 2589 3350 4691 1116
Size: 1473 761 1341 1116
Fragments arranged by size:
1473 1341 1116 761
BclI T'GATC_A
Cuts at: 1160 2211 1160
Size: 1051 3640
BfrBI ATG'CAT
Cuts at: 1272 1272
Size: 4691
BglI GCCn_nnn'nGGC
Cuts at: 1207 1207
Size: 4691
BglII A'GATC_T
Cuts at: 2206 4243 2206
Size: 2037 2654
BlpI GC'TnA_GC
Cuts at: 1803 1803
Size: 4691
Bme1580I G_kGCm'C
Cuts at: 362 1193 2316 2409 3465 362
Size: 831 1123 93 1056 1588
Fragments arranged by size:
1588 1123 1056 831 93
BmgBI CAC'GTC
Cuts at: 1134 1134
Size: 4691
BmrI ACTGGGnnnn_n'
Cuts at: 1903 1984 2319 4463 1903
Size: 81 335 2144 2131
Fragments arranged by size:
2144 2131 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 4691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 1354 116
Size: 1238 3453
Bpu10I CC'TnA_GC
Cuts at: 1154 1154
Size: 4691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1867 2746 3238 3536 1867
Size: 879 492 298 3022
Fragments arranged by size:
3022 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 576 4395 576
Size: 3819 872
BsaAI yAC'GTr
Cuts at: 2044 2044
Size: 4691
BsaBI GATnn'nnATC
Cuts at: 6 2205 4242 6
Size: 2199 2037 455
Fragments arranged by size:
2199 2037 455
BsaHI Gr'CG_yC
Cuts at: 2122 2370 3072 4568 2122
Size: 248 702 1496 2245
Fragments arranged by size:
2245 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1617 1880 2401 3079 3353 3500 3911 1617
Size: 263 521 678 274 147 411 2397
Fragments arranged by size:
2397 678 521 411 274 263 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 320 1142 1349 1352 320
Size: 822 207 3 3659
Fragments arranged by size:
3659 822 207 3
BseYI C'CCAG_C
Cuts at: 137 477 1192 2116 2874 3451 3947 137
Size: 340 715 924 758 577 496 881
Fragments arranged by size:
924 881 758 715 577 496 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1617 1538
Size: 79 4612
BsiEI CG_ry'CG
Cuts at: 104 2233 2279 3487 104
Size: 2129 46 1208 1308
Fragments arranged by size:
2129 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 362 675 1705 2483 2673 3465 362
Size: 313 1030 778 190 792 1588
Fragments arranged by size:
1588 1030 792 778 313 190
Bsp1286I G_dGCh'C
Cuts at: 362 675 890 1193 1705 2316 2409 2483 2673
Size: 313 215 303 512 611 93 74 190
Cuts at: 2673 2735 3465 362
Size: 62 730 1588
Fragments arranged by size:
1588 730 611 512 313 303 215 190
93 74 62
BspEI T'CCGG_A
Cuts at: 3911 3911
Size: 4691
BspHI T'CATG_A
Cuts at: 3867 3867
Size: 4691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1452 1741 1955 2257 2638 1452
Size: 289 214 302 381 3505
Fragments arranged by size:
3505 381 302 289 214
BsrBI CCG'CTC
Cuts at: 50 1422 2983 3037 4521 4686 50
Size: 1372 1561 54 1484 165 55
Fragments arranged by size:
1561 1484 1372 165 55 54
BsrDI GCAATG_nn'
Cuts at: 708 1580 2603 708
Size: 872 1023 2796
Fragments arranged by size:
2796 1023 872
BsrFI r'CCGG_y
Cuts at: 1617 2689 2870 3079 1617
Size: 1072 181 209 3229
Fragments arranged by size:
3229 1072 209 181
BssSI C'ACGA_G
Cuts at: 2962 3320 2962
Size: 358 4333
BstAPI GCAn_nnn'nTGC
Cuts at: 2068 2068
Size: 4691
BstBI TT'CG_AA
Cuts at: 1168 3052 1168
Size: 1884 2807
BstEII G'GTnAC_C
Cuts at: 382 382
Size: 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 477 477
Size: 4691
BstYI r'GATC_y
Cuts at: 1 876 1716 2183 2206 2541 2787 3799 3885
Size: 875 840 467 23 335 246 1012 86
Cuts at: 3885 3897 4243 1
Size: 12 346 449
Fragments arranged by size:
1012 875 840 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1185 107
Size: 1078 3613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 800 2641 2822 2985 800
Size: 1841 181 163 2506
Fragments arranged by size:
2506 1841 181 163
BtsI GCAGTG_nn'
Cuts at: 493 1934 493
Size: 1441 3250
DraI TTT'AAA
Cuts at: 1690 1690
Size: 4691
DrdI GACnn_nn'nnGTC
Cuts at: 2397 3255 2397
Size: 858 3833
EaeI y'GGCC_r
Cuts at: 636 2276 2450 2841 2868 4213 636
Size: 1640 174 391 27 1345 1114
Fragments arranged by size:
1640 1345 1114 391 174 27
EagI C'GGCC_G
Cuts at: 2276 2276
Size: 4691
EarI CTCTTCn'nnn_
Cuts at: 1013 1391 1514 2714 2924 1013
Size: 378 123 1200 210 2780
Fragments arranged by size:
2780 1200 378 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2899 3209 3355 4501 2899
Size: 310 146 1146 3089
Fragments arranged by size:
3089 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1703 1703
Size: 4691
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 1093 1354 2516 2948 3695 3963 116
Size: 977 261 1162 432 747 268 844
Fragments arranged by size:
1162 977 844 747 432 268 261
EcoO109I rG'GnC_Cy
Cuts at: 172 1830 172
Size: 1658 3033
EcoRI G'AATT_C
Cuts at: 1695 1695
Size: 4691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4691
FspI TGC'GCA
Cuts at: 2472 2472
Size: 4691
HaeII r_GCGC'y
Cuts at: 2125 2373 3395 4374 2125
Size: 248 1022 979 2442
Fragments arranged by size:
2442 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 778 810 2533 2563 2565 2595 778
Size: 32 1723 30 2 30 2874
Fragments arranged by size:
2874 1723 32 30 30 2
HincII GTy'rAC
Cuts at: 1131 1730 1131
Size: 599 4092
KasI G'GCGC_C
Cuts at: 2121 2369 2121
Size: 248 4443
KpnI G_GTAC'C
Cuts at: 1711 1711
Size: 4691
MluI A'CGCG_T
Cuts at: 4124 4124
Size: 4691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3362 3546 3362
Size: 184 4507
MscI TGG'CCA
Cuts at: 638 2452 638
Size: 1814 2877
MslI CAynn'nnrTG
Cuts at: 229 365 514 776 1348 2680 2807 229
Size: 136 149 262 572 1332 127 2113
Fragments arranged by size:
2113 1332 572 262 149 136 127
MspA1I CmG'CkG
Cuts at: 148 642 1158 1552 1802 2116 2476 3489 3734
Size: 494 516 394 250 314 360 1013 245
Cuts at: 3734 148
Size: 1105
Fragments arranged by size:
1105 1013 516 494 394 360 314 250
245
NaeI GCC'GGC
Cuts at: 2872 2872
Size: 4691
NarI GG'CG_CC
Cuts at: 2122 2370 2122
Size: 248 4443
NcoI C'CATG_G
Cuts at: 107 1185 107
Size: 1078 3613
NgoMIV G'CCGG_C
Cuts at: 2870 2870
Size: 4691
NsiI A_TGCA'T
Cuts at: 1274 1274
Size: 4691
NspI r_CATG'y
Cuts at: 228 1407 1659 1744 2244 3151 228
Size: 1179 252 85 500 907 1768
Fragments arranged by size:
1768 1179 907 500 252 85
PciI A'CATG_T
Cuts at: 224 3147 224
Size: 2923 1768
PflMI CCAn_nnn'nTGG
Cuts at: 354 1575 354
Size: 1221 3470
PmeI GTTT'AAAC
Cuts at: 1690 1690
Size: 4691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4659
PpuMI rG'GwC_Cy
Cuts at: 172 172
Size: 4691
PspOMI G'GGCC_C
Cuts at: 1189 1189
Size: 4691
PstI C_TGCA'G
Cuts at: 603 1645 1738 2423 603
Size: 1042 93 685 2871
Fragments arranged by size:
2871 1042 685 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4691
PvuII CAG'CTG
Cuts at: 642 1158 1552 2116 2476 642
Size: 516 394 564 360 2857
Fragments arranged by size:
2857 564 516 394 360
RsrII CG'GwC_CG
Cuts at: 2886 2886
Size: 4691
SacI G_AGCT'C
Cuts at: 1705 1705
Size: 4691
SalI G'TCGA_C
Cuts at: 1728 1728
Size: 4691
SapI GCTCTTCn'nnn_
Cuts at: 1013 2714 2924 1013
Size: 1701 210 2780
Fragments arranged by size:
2780 1701 210
SbfI CC_TGCA'GG
Cuts at: 1738 1738
Size: 4691
ScaI AGT'ACT
Cuts at: 4208 4208
Size: 4691
SfcI C'TryA_G
Cuts at: 33 599 1116 1334 1547 1641 1734 2419 3412
Size: 566 517 218 213 94 93 685 993
Cuts at: 3412 3603 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 566 517 218 213 191
94 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 1207 1207
Size: 4691
SfoI GGC'GCC
Cuts at: 2123 2371 2123
Size: 248 4443
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
SmaI CCC'GGG
Cuts at: 1713 1713
Size: 4691
SmlI C'TyrA_G
Cuts at: 1846 2761 3253 3515 1846
Size: 915 492 262 3022
Fragments arranged by size:
3022 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4691
SphI G_CATG'C
Cuts at: 1659 1744 2244 1659
Size: 85 500 4106
Fragments arranged by size:
4106 500 85
StyI C'CwwG_G
Cuts at: 107 586 1185 1440 1825 2179 107
Size: 479 599 255 385 354 2619
Fragments arranged by size:
2619 599 479 385 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 1582 2274 2407 2866 3075 1500
Size: 82 692 133 459 209 3116
Fragments arranged by size:
3116 692 459 209 133 82
TatI w'GTAC_w
Cuts at: 551 4206 551
Size: 3655 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 4659
Tth111I GACn'n_nGTC
Cuts at: 2488 2488
Size: 4691
XbaI T'CTAG_A
Cuts at: 1722 1722
Size: 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 280 2186 280
Size: 1906 2785
XmaI C'CCGG_G
Cuts at: 1711 1711
Size: 4691
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BcgI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI