Restriction map for
pF1KA0800
(Circular) (Six-base) MAPSORT of: pF1KA0800.seq Check: 4205 from: 1 to: 7634
pF1KA0800 7634 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3190 3406 3451 3190
Size: 216 45 7373
Fragments arranged by size:
7373 216 45
AccI GT'mk_AC
Cuts at: 4672 4672
Size: 7634
Acc65I G'GTAC_C
Cuts at: 201 4650 201
Size: 4449 3185
AclI AA'CG_TT
Cuts at: 7474 7474
Size: 7634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1213 1454 1720 1892 2137 3478 5459 5891
Size: 947 241 266 172 245 1341 1981 432
Cuts at: 5891 6244 6906 266
Size: 353 662 994
Fragments arranged by size:
1981 1341 994 947 662 432 353 266
245 241 172
AflIII A'CryG_T
Cuts at: 126 2042 4986 6786 7067 126
Size: 1916 2944 1800 281 693
Fragments arranged by size:
2944 1916 1800 693 281
AgeI A'CCGG_T
Cuts at: 1339 1339
Size: 7634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1693 2332 1693
Size: 639 6995
AlwNI CAG_nnn'CTG
Cuts at: 2456 2472 2779 4287 4404 4939 6377 2456
Size: 16 307 1508 117 535 1438 3713
Fragments arranged by size:
3713 1508 1438 535 307 117 16
ApaI G_GGCC'C
Cuts at: 516 2376 516
Size: 1860 5774
ApaLI G'TGCA_C
Cuts at: 1613 6472 1613
Size: 4859 2775
ApoI r'AATT_y
Cuts at: 609 1264 1276 1596 1825 2135 2209 4638 6987
Size: 655 12 320 229 310 74 2429 2349
Cuts at: 6987 7243 609
Size: 256 1000
Fragments arranged by size:
2429 2349 1000 655 320 310 256 229
74 12
AseI AT'TA_AT
Cuts at: 20 3932 20
Size: 3912 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7634
AvaI C'yCGr_G
Cuts at: 515 4654 515
Size: 4139 3495
BamHI G'GATC_C
Cuts at: 264 4659 264
Size: 4395 3239
BanI G'GyrC_C
Cuts at: 201 1045 2004 2866 3052 3326 3439 4650 5064
Size: 844 959 862 186 274 113 1211 414
Cuts at: 5064 5312 5347 201
Size: 248 35 2488
Fragments arranged by size:
2488 1211 959 862 844 414 274 248
186 113 35
BanII G_rGCy'C
Cuts at: 149 404 516 1535 2376 4648 5678 149
Size: 255 112 1019 841 2272 1030 2105
Fragments arranged by size:
2272 2105 1030 1019 841 255 112
BbeI G_GCGC'C
Cuts at: 5068 5316 5068
Size: 248 7386
BbsI GAAGACnn'nnnn_
Cuts at: 645 1546 1945 4535 645
Size: 901 399 2590 3744
Fragments arranged by size:
3744 2590 901 399
BbvCI CC'TCA_GC
Cuts at: 2105 2900 2105
Size: 795 6839
BciVI GTATCCnnnnn_n'
Cuts at: 5532 6588 7634 5532
Size: 1056 1046 5532
Fragments arranged by size:
5532 1056 1046
BclI T'GATC_A
Cuts at: 1574 3926 5154 1574
Size: 2352 1228 4054
Fragments arranged by size:
4054 2352 1228
BfrBI ATG'CAT
Cuts at: 379 1404 379
Size: 1025 6609
BglI GCCn_nnn'nGGC
Cuts at: 520 520
Size: 7634
BglII A'GATC_T
Cuts at: 821 3759 5149 7186 821
Size: 2938 1390 2037 1269
Fragments arranged by size:
2938 2037 1390 1269
BlpI GC'TnA_GC
Cuts at: 143 4746 143
Size: 4603 3031
Bme1580I G_kGCm'C
Cuts at: 516 1617 2376 5259 5352 6476 516
Size: 1101 759 2883 93 1124 1674
Fragments arranged by size:
2883 1674 1124 1101 759 93
BmgBI CAC'GTC
Cuts at: 2043 2043
Size: 7634
BmrI ACTGGGnnnn_n'
Cuts at: 689 1664 2619 2767 4846 4927 5262 7406 689
Size: 975 955 148 2079 81 335 2144 917
Fragments arranged by size:
2144 2079 975 955 917 335 148 81
BmtI G_CTAG'C
Cuts at: 1646 2929 4492 1646
Size: 1283 1563 4788
Fragments arranged by size:
4788 1563 1283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6001 6001
Size: 7634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 830 2005 3940 4426 181
Size: 649 1175 1935 486 3389
Fragments arranged by size:
3389 1935 1175 649 486
Bpu10I CC'TnA_GC
Cuts at: 2105 2900 3221 2105
Size: 795 321 6518
Fragments arranged by size:
6518 795 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 5689 6403 6701 4810
Size: 879 714 298 5743
Fragments arranged by size:
5743 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 785 7338 785
Size: 6553 1081
BsaAI yAC'GTr
Cuts at: 4987 4987
Size: 7634
BsaBI GATnn'nnATC
Cuts at: 6 287 983 2161 2347 5148 7185 6
Size: 281 696 1178 186 2801 2037 455
Fragments arranged by size:
2801 2037 1178 696 455 281 186
BsaHI Gr'CG_yC
Cuts at: 3830 5065 5313 6015 7511 3830
Size: 1235 248 702 1496 3953
Fragments arranged by size:
3953 1496 1235 702 248
BsaWI w'CCGG_w
Cuts at: 1339 4823 5344 6022 6433 6580 6854 1339
Size: 3484 521 678 411 147 274 2119
Fragments arranged by size:
3484 2119 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1082 1112 4399 4429 1082
Size: 30 3287 30 4287
Fragments arranged by size:
4287 3287 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 2216 3197 3502 4324 4435 4498 4519 4561
Size: 1462 981 305 822 111 63 21 42
Cuts at: 4561 754
Size: 3827
Fragments arranged by size:
3827 1462 981 822 305 111 63 42
21
BseYI C'CCAG_C
Cuts at: 318 1845 3069 5059 5817 6482 6890 318
Size: 1527 1224 1990 758 665 408 1062
Fragments arranged by size:
1990 1527 1224 1062 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3429 3575 3836 3429
Size: 146 261 7227
Fragments arranged by size:
7227 261 146
BsiEI CG_ry'CG
Cuts at: 104 5176 5222 6452 104
Size: 5072 46 1230 1286
Fragments arranged by size:
5072 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 149 1535 1617 2267 2321 4648 5426 5616 6476
Size: 1386 82 650 54 2327 778 190 860
Cuts at: 6476 149
Size: 1307
Fragments arranged by size:
2327 1386 1307 860 778 650 190 82
54
BsmI GAATG_Cn'
Cuts at: 369 381 1754 3406 3627 369
Size: 12 1373 1652 221 4376
Fragments arranged by size:
4376 1652 1373 221 12
Bsp1286I G_dGCh'C
Cuts at: 149 404 516 1535 1617 2267 2321 2376 4648
Size: 255 112 1019 82 650 54 55 2272
Cuts at: 4648 5259 5352 5426 5616 5678 6476 149
Size: 611 93 74 190 62 798 1307
Fragments arranged by size:
2272 1307 1019 798 650 611 255 190
112 93 82 74 62 55 54
BspHI T'CATG_A
Cuts at: 359 1800 6066 359
Size: 1441 4266 1927
Fragments arranged by size:
4266 1927 1441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1675 2741 3190 3406 3451 4684 4898 5200 5581
Size: 1066 449 216 45 1233 214 302 381
Cuts at: 5581 1675
Size: 3728
Fragments arranged by size:
3728 1233 1066 449 381 302 216 214
45
BsrBI CCG'CTC
Cuts at: 50 3423 3848 3985 5926 5980 7464 7629 50
Size: 3373 425 137 1941 54 1484 165 55
Fragments arranged by size:
3373 1941 1484 425 165 137 55 54
BsrDI GCAATG_nn'
Cuts at: 584 2831 3015 3701 5546 584
Size: 2247 184 686 1845 2672
Fragments arranged by size:
2672 2247 1845 686 184
BsrFI r'CCGG_y
Cuts at: 1339 5632 5813 1339
Size: 4293 181 3160
Fragments arranged by size:
4293 3160 181
BsrGI T'GTAC_A
Cuts at: 4180 4180
Size: 7634
BssSI C'ACGA_G
Cuts at: 5905 6613 5905
Size: 708 6926
BstAPI GCAn_nnn'nTGC
Cuts at: 5011 5011
Size: 7634
BstBI TT'CG_AA
Cuts at: 636 3954 5995 636
Size: 3318 2041 2275
Fragments arranged by size:
3318 2275 2041
BstXI CCAn_nnnn'nTGG
Cuts at: 1713 3913 1713
Size: 2200 5434
BstYI r'GATC_y
Cuts at: 1 264 821 2059 2462 2633 3759 4659 5126
Size: 263 557 1238 403 171 1126 900 467
Cuts at: 5126 5149 5484 5730 6036 6048 6134 7186 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1238 1126 1052 900 557 467 449 403
335 306 263 246 171 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 884 884
Size: 7634
BtgI C'CryG_G
Cuts at: 700 1385 2905 3048 700
Size: 685 1520 143 5286
Fragments arranged by size:
5286 1520 685 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5584 5765 5928 5584
Size: 181 163 7290
Fragments arranged by size:
7290 181 163
BtsI GCAGTG_nn'
Cuts at: 172 192 413 2265 2511 2777 4877 172
Size: 20 221 1852 246 266 2100 2929
Fragments arranged by size:
2929 2100 1852 266 246 221 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1260 1295 1260
Size: 35 7599
DraI TTT'AAA
Cuts at: 410 4633 410
Size: 4223 3411
DrdI GACnn_nn'nnGTC
Cuts at: 293 5340 6684 293
Size: 5047 1344 1243
Fragments arranged by size:
5047 1344 1243
EaeI y'GGCC_r
Cuts at: 1703 2273 5219 5393 5784 5811 7156 1703
Size: 570 2946 174 391 27 1345 2181
Fragments arranged by size:
2946 2181 1345 570 391 174 27
EagI C'GGCC_G
Cuts at: 5219 5219
Size: 7634
EarI CTCTTCn'nnn_
Cuts at: 242 346 717 4070 4301 4316 5657 5867 242
Size: 104 371 3353 231 15 1341 210 2009
Fragments arranged by size:
3353 2009 1341 371 231 210 104 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3326 4426 5842 6584 6730 7444 3326
Size: 1100 1416 742 146 714 3516
Fragments arranged by size:
3516 1416 1100 742 714 146
EcoICRI GAG'CTC
Cuts at: 147 1533 4646 147
Size: 1386 3113 3135
Fragments arranged by size:
3135 3113 1386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 266 830 1213 1454 1720 1892 2005 2137
Size: 85 564 383 241 266 172 113 132
Cuts at: 2137 3478 3940 4426 5459 5891 6244 6906 181
Size: 1341 462 486 1033 432 353 662 909
Fragments arranged by size:
1341 1033 909 662 564 486 462 432
383 353 266 241 172 132 113 85
EcoNI CCTnn'n_nnAGG
Cuts at: 4501 4501
Size: 7634
EcoO109I rG'GnC_Cy
Cuts at: 2373 3548 3948 4773 2373
Size: 1175 400 825 5234
Fragments arranged by size:
5234 1175 825 400
EcoRI G'AATT_C
Cuts at: 4638 4638
Size: 7634
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 3648 5653 2674
Size: 974 2005 4655
Fragments arranged by size:
4655 2005 974
FspI TGC'GCA
Cuts at: 1286 1681 5415 1286
Size: 395 3734 3505
Fragments arranged by size:
3734 3505 395
HaeII r_GCGC'y
Cuts at: 3247 5068 5316 6546 7317 3247
Size: 1821 248 1230 771 3564
Fragments arranged by size:
3564 1821 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1082 1114 2044 2051 2076 2083 2983 3015 3832
Size: 32 930 7 25 7 900 32 817
Cuts at: 3832 3864 4516 4548 5476 5506 5508 5538 1082
Size: 32 652 32 928 30 2 30 3178
Fragments arranged by size:
3178 930 928 900 817 652 32 32
32 32 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 3743 4673 3743
Size: 930 6704
HindIII A'AGCT_T
Cuts at: 803 803
Size: 7634
KasI G'GCGC_C
Cuts at: 5064 5312 5064
Size: 248 7386
KpnI G_GTAC'C
Cuts at: 205 4654 205
Size: 4449 3185
MfeI C'AATT_G
Cuts at: 226 1932 2166 226
Size: 1706 234 5694
Fragments arranged by size:
5694 1706 234
MluI A'CGCG_T
Cuts at: 7067 7067
Size: 7634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2324 3023 4397 6393 6577 2027
Size: 297 699 1374 1996 184 3084
Fragments arranged by size:
3084 1996 1374 699 297 184
MscI TGG'CCA
Cuts at: 1705 2275 5395 1705
Size: 570 3120 3944
Fragments arranged by size:
3944 3120 570
MslI CAynn'nnrTG
Cuts at: 185 1147 2314 2341 3713 3911 4153 5623 5750
Size: 962 1167 27 1372 198 242 1470 127
Cuts at: 5750 185
Size: 2069
Fragments arranged by size:
2069 1470 1372 1167 962 242 198 127
27
MspA1I CmG'CkG
Cuts at: 618 1870 2456 2472 3087 3456 4745 5059 5419
Size: 1252 586 16 615 369 1289 314 360
Cuts at: 5419 6203 6448 618
Size: 784 245 1804
Fragments arranged by size:
1804 1289 1252 784 615 586 369 360
314 245 16
NaeI GCC'GGC
Cuts at: 5815 5815
Size: 7634
NarI GG'CG_CC
Cuts at: 5065 5313 5065
Size: 248 7386
NcoI C'CATG_G
Cuts at: 1385 3048 1385
Size: 1663 5971
NdeI CA'TA_TG
Cuts at: 381 381
Size: 7634
NgoMIV G'CCGG_C
Cuts at: 5813 5813
Size: 7634
NheI G'CTAG_C
Cuts at: 1642 2925 4488 1642
Size: 1283 1563 4788
Fragments arranged by size:
4788 1563 1283
NsiI A_TGCA'T
Cuts at: 381 1406 381
Size: 1025 6609
NspI r_CATG'y
Cuts at: 130 993 1404 1790 3157 4687 5187 6790 130
Size: 863 411 386 1367 1530 500 1603 974
Fragments arranged by size:
1603 1530 1367 974 863 500 411 386
PasI CC'CwG_GG
Cuts at: 1034 2916 1034
Size: 1882 5752
PciI A'CATG_T
Cuts at: 126 6786 126
Size: 6660 974
PflMI CCAn_nnn'nTGG
Cuts at: 2282 3295 4404 2282
Size: 1013 1109 5512
Fragments arranged by size:
5512 1109 1013
PfoI T'CCnGG_A
Cuts at: 3365 3365
Size: 7634
PmeI GTTT'AAAC
Cuts at: 4633 4633
Size: 7634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2130 6080 6112 2098
Size: 32 3950 32 3620
Fragments arranged by size:
3950 3620 32 32
PpuMI rG'GwC_Cy
Cuts at: 3548 3948 3548
Size: 400 7234
PspOMI G'GGCC_C
Cuts at: 512 2372 512
Size: 1860 5774
PstI C_TGCA'G
Cuts at: 420 2495 2606 4681 5366 420
Size: 2075 111 2075 685 2688
Fragments arranged by size:
2688 2075 2075 685 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7634
PvuII CAG'CTG
Cuts at: 618 1870 2456 2472 3087 3456 5059 5419 618
Size: 1252 586 16 615 369 1603 360 2833
Fragments arranged by size:
2833 1603 1252 615 586 369 360 16
RsrII CG'GwC_CG
Cuts at: 5829 5829
Size: 7634
SacI G_AGCT'C
Cuts at: 149 1535 4648 149
Size: 1386 3113 3135
Fragments arranged by size:
3135 3113 1386
SalI G'TCGA_C
Cuts at: 4671 4671
Size: 7634
SapI GCTCTTCn'nnn_
Cuts at: 5657 5867 5657
Size: 210 7424
SbfI CC_TGCA'GG
Cuts at: 4681 4681
Size: 7634
ScaI AGT'ACT
Cuts at: 1581 7151 1581
Size: 5570 2064
SfcI C'TryA_G
Cuts at: 33 113 416 597 652 658 773 2063 2075
Size: 80 303 181 55 6 115 1290 12
Cuts at: 2075 2491 2602 3057 3803 4139 4677 5362 6330
Size: 416 111 455 746 336 538 685 968
Cuts at: 6330 6521 33
Size: 191 1146
Fragments arranged by size:
1290 1146 968 746 685 538 455 416
336 303 191 181 115 111 80 55
12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 520 520
Size: 7634
SfoI GGC'GCC
Cuts at: 5066 5314 5066
Size: 248 7386
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7634
SmaI CCC'GGG
Cuts at: 4656 4656
Size: 7634
SmlI C'TyrA_G
Cuts at: 4789 5704 6418 6680 4789
Size: 915 714 262 5743
Fragments arranged by size:
5743 915 714 262
SpeI A'CTAG_T
Cuts at: 67 853 67
Size: 786 6848
SphI G_CATG'C
Cuts at: 1404 1790 4687 5187 1404
Size: 386 2897 500 3851
Fragments arranged by size:
3851 2897 500 386
SspI AAT'ATT
Cuts at: 1105 1637 1105
Size: 532 7102
StyI C'CwwG_G
Cuts at: 1385 3048 4768 5122 1385
Size: 1663 1720 354 3897
Fragments arranged by size:
3897 1720 1663 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1026 1781 2854 5217 5350 5809 6018 1026
Size: 755 1073 2363 133 459 209 2642
Fragments arranged by size:
2642 2363 1073 755 459 209 133
TatI w'GTAC_w
Cuts at: 1296 1579 4180 7149 1296
Size: 283 2601 2969 1781
Fragments arranged by size:
2969 2601 1781 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1734 1766 3783 3815 3845 3877 3908 3940 7151
Size: 32 2017 32 30 32 31 32 3211
Cuts at: 7151 7183 1734
Size: 32 2185
Fragments arranged by size:
3211 2185 2017 32 32 32 32 32
31 30
Tth111I GACn'n_nGTC
Cuts at: 2512 5431 2512
Size: 2919 4715
XbaI T'CTAG_A
Cuts at: 4500 4665 4500
Size: 165 7469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 957 5129 957
Size: 4172 3462
XmaI C'CCGG_G
Cuts at: 4654 4654
Size: 7634
XmnI GAAnn'nnTTC
Cuts at: 502 502
Size: 7634
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BsiWI BsmBI BspEI BssHII BstEII BstZ17I
ClaI DraIII EcoRV FseI FspAI HpaI NotI NruI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI