Restriction map for
pF1KA0799
(Circular) (Six-base) MAPSORT of: pF1KA0799.seq Check: 6072 from: 1 to: 9287
pF1KA0799 9287 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2754 2754
Size: 9287
AatII G_ACGT'C
Cuts at: 2204 2204
Size: 9287
AccI GT'mk_AC
Cuts at: 1104 1292 5360 6053 6325 1104
Size: 188 4068 693 272 4066
Fragments arranged by size:
4068 4066 693 272 188
Acc65I G'GTAC_C
Cuts at: 6303 6303
Size: 9287
AclI AA'CG_TT
Cuts at: 9127 9127
Size: 9287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 3042 3179 4369 4786 7112 7544 8291 8559
Size: 1577 137 1190 417 2326 432 747 268
Cuts at: 8559 1465
Size: 2193
Fragments arranged by size:
2326 2193 1577 1190 747 432 417 268
137
AfeI AGC'GCT
Cuts at: 3817 6238 3817
Size: 2421 6866
AflII C'TTAA_G
Cuts at: 5134 5134
Size: 9287
AflIII A'CryG_T
Cuts at: 1875 4034 5327 5837 6639 7743 8720 1875
Size: 2159 1293 510 802 1104 977 2442
Fragments arranged by size:
2442 2159 1293 1104 977 802 510
AgeI A'CCGG_T
Cuts at: 3509 7675 3509
Size: 4166 5121
AhdI GACnn_n'nnGTC
Cuts at: 312 1389 312
Size: 1077 8210
AleI CACnn'nnGTG
Cuts at: 3226 4267 3226
Size: 1041 8246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2239 3277 5041 2239
Size: 1038 1764 6485
Fragments arranged by size:
6485 1764 1038
AlwNI CAG_nnn'CTG
Cuts at: 2307 2832 2862 4448 4632 4842 5001 5619 5832
Size: 525 30 1586 184 210 159 618 213
Cuts at: 5832 6592 8159 2307
Size: 760 1567 3435
Fragments arranged by size:
3435 1586 1567 760 618 525 213 210
184 159 30
ApaI G_GGCC'C
Cuts at: 2361 2361
Size: 9287
ApaLI G'TGCA_C
Cuts at: 2214 8057 2214
Size: 5843 3444
ApoI r'AATT_y
Cuts at: 180 937 1126 3004 4282 5455 5896 6291 8640
Size: 757 189 1878 1278 1173 441 395 2349
Cuts at: 8640 8896 180
Size: 256 571
Fragments arranged by size:
2349 1878 1278 1173 757 571 441 395
256 189
AseI AT'TA_AT
Cuts at: 20 20
Size: 9287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9287
AvaI C'yCGr_G
Cuts at: 871 2932 3498 5168 5867 6307 871
Size: 2061 566 1670 699 440 3851
Fragments arranged by size:
3851 2061 1670 699 566 440
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5599 5632 5599
Size: 33 9254
BamHI G'GATC_C
Cuts at: 5794 6312 5794
Size: 518 8769
BanI G'GyrC_C
Cuts at: 476 3228 3403 3502 3883 4099 6117 6303 6717
Size: 2752 175 99 381 216 2018 186 414
Cuts at: 6717 6965 7000 476
Size: 248 35 2763
Fragments arranged by size:
2763 2752 2018 414 381 248 216 186
175 99 35
BanII G_rGCy'C
Cuts at: 329 2361 2651 2888 3784 6301 7331 329
Size: 2032 290 237 896 2517 1030 2285
Fragments arranged by size:
2517 2285 2032 1030 896 290 237
BbeI G_GCGC'C
Cuts at: 480 6721 6969 480
Size: 6241 248 2798
Fragments arranged by size:
6241 2798 248
BbsI GAAGACnn'nnnn_
Cuts at: 198 679 745 1001 2142 2275 4328 6078 198
Size: 481 66 256 1141 133 2053 1750 3407
Fragments arranged by size:
3407 2053 1750 1141 481 256 133 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2195 2208 2229 2242 2195
Size: 13 21 13 9240
Fragments arranged by size:
9240 21 13 13
BciVI GTATCCnnnnn_n'
Cuts at: 1893 5066 7185 7946 9287 1893
Size: 3173 2119 761 1341 1893
Fragments arranged by size:
3173 2119 1893 1341 761
BclI T'GATC_A
Cuts at: 4169 5945 6807 4169
Size: 1776 862 6649
Fragments arranged by size:
6649 1776 862
BfrBI ATG'CAT
Cuts at: 4065 4065
Size: 9287
BglI GCCn_nnn'nGGC
Cuts at: 485 5231 485
Size: 4746 4541
BglII A'GATC_T
Cuts at: 1177 6802 8839 1177
Size: 5625 2037 1625
Fragments arranged by size:
5625 2037 1625
BlpI GC'TnA_GC
Cuts at: 1984 6399 1984
Size: 4415 4872
Bme1580I G_kGCm'C
Cuts at: 1149 2218 2361 3233 3886 4102 4961 6120 6912
Size: 1069 143 872 653 216 859 1159 792
Cuts at: 6912 7005 8061 1149
Size: 93 1056 2375
Fragments arranged by size:
2375 1159 1069 1056 872 859 792 653
216 143 93
BmgBI CAC'GTC
Cuts at: 1050 5358 1050
Size: 4308 4979
BmrI ACTGGGnnnn_n'
Cuts at: 3236 4432 6499 6580 6915 9059 3236
Size: 1196 2067 81 335 2144 3464
Fragments arranged by size:
3464 2144 2067 1196 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5059 7654 5059
Size: 2595 6692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2116 2329 2761 2806 2917 3291 4864 5572
Size: 338 213 432 45 111 374 1573 708
Cuts at: 5572 5596 5758 6261 1778
Size: 24 162 503 4804
Fragments arranged by size:
4804 1573 708 503 432 374 338 213
162 111 45 24
Bpu10I CC'TnA_GC
Cuts at: 5011 5011
Size: 9287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1631 3688 3733 3862 5029 5038 5671 6463
Size: 1027 2057 45 129 1167 9 633 792
Cuts at: 6463 7342 7834 8132 604
Size: 879 492 298 1759
Fragments arranged by size:
2057 1759 1167 1027 879 792 633 492
298 129 45 9
BsaI GGTCTCn'nnnn_
Cuts at: 8991 8991
Size: 9287
BsaAI yAC'GTr
Cuts at: 6134 6640 6134
Size: 506 8781
BsaBI GATnn'nnATC
Cuts at: 6 3946 5251 6801 8838 6
Size: 3940 1305 1550 2037 455
Fragments arranged by size:
3940 2037 1550 1305 455
BsaHI Gr'CG_yC
Cuts at: 313 477 2201 3113 6041 6718 6966 7668 9164
Size: 164 1724 912 2928 677 248 702 1496
Cuts at: 9164 313
Size: 436
Fragments arranged by size:
2928 1724 1496 912 702 677 436 248
164
BsaWI w'CCGG_w
Cuts at: 209 2969 3176 3509 5786 6476 6997 7675 7949
Size: 2760 207 333 2277 690 521 678 274
Cuts at: 7949 8096 8507 209
Size: 147 411 989
Fragments arranged by size:
2760 2277 989 690 678 521 411 333
274 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 1595 1625 2893 2923 3289 3319 3340
Size: 30 1183 30 1268 30 366 30 21
Cuts at: 3340 3370 4837 4867 6031 6061 382
Size: 30 1467 30 1164 30 3608
Fragments arranged by size:
3608 1467 1268 1183 1164 366 30 30
30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 1980 2466 2469 2914 3052 3091 3094 3214
Size: 1673 486 3 445 138 39 3 120
Cuts at: 3214 3294 3774 3883 4231 5833 5836 5850 307
Size: 80 480 109 348 1602 3 14 3744
Fragments arranged by size:
3744 1673 1602 486 480 445 348 138
120 109 80 39 14 3 3 3
BseYI C'CCAG_C
Cuts at: 2119 2262 2657 3136 3343 4625 5272 6712 7470
Size: 143 395 479 207 1282 647 1440 758
Cuts at: 7470 8047 8543 2119
Size: 577 496 2863
Fragments arranged by size:
2863 1440 1282 758 647 577 496 479
395 207 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 2931 3298 4177 5001 5623 209
Size: 2722 367 879 824 622 3873
Fragments arranged by size:
3873 2722 879 824 622 367
BsiEI CG_ry'CG
Cuts at: 104 4613 6829 6875 8083 104
Size: 4509 2216 46 1208 1308
Fragments arranged by size:
4509 2216 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 250 329 2218 2651 2888 3146 6301 7079 7269
Size: 79 1889 433 237 258 3155 778 190
Cuts at: 7269 8061 250
Size: 792 1476
Fragments arranged by size:
3155 1889 1476 792 778 433 258 237
190 79
BsmI GAATG_Cn'
Cuts at: 1585 1965 2440 4095 1585
Size: 380 475 1655 6777
Fragments arranged by size:
6777 1655 475 380
BsmBI CGTCTCn'nnnn_
Cuts at: 6051 6051
Size: 9287
Bsp1286I G_dGCh'C
Cuts at: 250 329 1149 2218 2361 2651 2888 3146 3233
Size: 79 820 1069 143 290 237 258 87
Cuts at: 3233 3784 3886 4102 4961 6120 6301 6912 7005
Size: 551 102 216 859 1159 181 611 93
Cuts at: 7005 7079 7269 7331 8061 250
Size: 74 190 62 730 1476
Fragments arranged by size:
1476 1159 1069 859 820 730 611 551
290 258 237 216 190 181 143 102
93 87 79 74 62
BspEI T'CCGG_A
Cuts at: 209 2969 3176 5786 8507 209
Size: 2760 207 2610 2721 989
Fragments arranged by size:
2760 2721 2610 989 207
BspHI T'CATG_A
Cuts at: 6203 8463 6203
Size: 2260 7027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 580 2754 2813 4910 5453 6337 6551 6853 7234
Size: 2174 59 2097 543 884 214 302 381
Cuts at: 7234 580
Size: 2633
Fragments arranged by size:
2633 2174 2097 884 543 381 302 214
59
BsrBI CCG'CTC
Cuts at: 50 2871 2982 3731 4737 7579 7633 9117 9282
Size: 2821 111 749 1006 2842 54 1484 165
Cuts at: 9282 50
Size: 55
Fragments arranged by size:
2842 2821 1484 1006 749 165 111 55
54
BsrDI GCAATG_nn'
Cuts at: 738 1049 1383 7199 738
Size: 311 334 5816 2826
Fragments arranged by size:
5816 2826 334 311
BsrFI r'CCGG_y
Cuts at: 473 3509 3599 4532 7285 7466 7675 473
Size: 3036 90 933 2753 181 209 2085
Fragments arranged by size:
3036 2753 2085 933 209 181 90
BsrGI T'GTAC_A
Cuts at: 746 3614 3653 4985 746
Size: 2868 39 1332 5048
Fragments arranged by size:
5048 2868 1332 39
BssSI C'ACGA_G
Cuts at: 2217 7558 7916 2217
Size: 5341 358 3588
Fragments arranged by size:
5341 3588 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2037 2862 4902 6664 2037
Size: 825 2040 1762 4660
Fragments arranged by size:
4660 2040 1762 825
BstBI TT'CG_AA
Cuts at: 3098 7648 3098
Size: 4550 4737
BstXI CCAn_nnnn'nTGG
Cuts at: 3018 3343 5937 6183 3018
Size: 325 2594 246 6122
Fragments arranged by size:
6122 2594 325 246
BstYI r'GATC_y
Cuts at: 1 1177 1259 2774 4052 4997 5794 6312 6779
Size: 1176 82 1515 1278 945 797 518 467
Cuts at: 6779 6802 7137 7383 8395 8481 8493 8839 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1515 1278 1176 1012 945 797 518 467
449 346 335 246 86 82 23 12
BstZ17I GTA'TAC
Cuts at: 1105 1105
Size: 9287
Bsu36I CC'TnA_GG
Cuts at: 1246 2195 5773 6011 1246
Size: 949 3578 238 4522
Fragments arranged by size:
4522 3578 949 238
BtgI C'CryG_G
Cuts at: 107 330 458 1313 2345 4040 5213 5226 5309
Size: 223 128 855 1032 1695 1173 13 83
Cuts at: 5309 5449 5556 5798 5924 107
Size: 140 107 242 126 3470
Fragments arranged by size:
3470 1695 1173 1032 855 242 223 140
128 126 107 83 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 417 498 3200 7237 7418 7581 417
Size: 81 2702 4037 181 163 2123
Fragments arranged by size:
4037 2702 2123 181 163 81
BtsI GCAGTG_nn'
Cuts at: 901 1133 1960 4194 4977 6530 901
Size: 232 827 2234 783 1553 3658
Fragments arranged by size:
3658 2234 1553 827 783 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5982 6017 5982
Size: 35 9252
DraI TTT'AAA
Cuts at: 6286 6286
Size: 9287
DraIII CAC_nnn'GTG
Cuts at: 4568 4568
Size: 9287
DrdI GACnn_nn'nnGTC
Cuts at: 2965 6993 7851 2965
Size: 4028 858 4401
Fragments arranged by size:
4401 4028 858
EaeI y'GGCC_r
Cuts at: 156 1310 2342 3147 3956 4178 5306 5352 5559
Size: 1154 1032 805 809 222 1128 46 207
Cuts at: 5559 5927 6194 6872 7046 7437 7464 8809 156
Size: 368 267 678 174 391 27 1345 634
Fragments arranged by size:
1345 1154 1128 1032 809 805 678 634
391 368 267 222 207 174 46 27
EagI C'GGCC_G
Cuts at: 6872 6872
Size: 9287
EarI CTCTTCn'nnn_
Cuts at: 1250 2318 2576 2631 2701 2765 3198 3548 5122
Size: 1068 258 55 70 64 433 350 1574
Cuts at: 5122 5657 5737 7310 7520 1250
Size: 535 80 1573 210 3017
Fragments arranged by size:
3017 1574 1573 1068 535 433 350 258
210 80 70 64 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3820 5763 5793 6246 7495 7805 7951 9097 3820
Size: 1943 30 453 1249 310 146 1146 4010
Fragments arranged by size:
4010 1943 1249 1146 453 310 146 30
EcoICRI GAG'CTC
Cuts at: 327 2649 2886 6299 327
Size: 2322 237 3413 3315
Fragments arranged by size:
3413 3315 2322 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 1778 2116 2329 2761 2806 2917 3042 3179
Size: 313 338 213 432 45 111 125 137
Cuts at: 3179 3291 4369 4786 4864 5572 5596 5758 6261
Size: 112 1078 417 78 708 24 162 503
Cuts at: 6261 7112 7544 8291 8559 1465
Size: 851 432 747 268 2193
Fragments arranged by size:
2193 1078 851 747 708 503 432 432
417 338 313 268 213 162 137 125
112 111 78 45 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2473 2473
Size: 9287
EcoO109I rG'GnC_Cy
Cuts at: 2400 2802 3204 3485 3744 4427 4605 5442 5768
Size: 402 402 281 259 683 178 837 326
Cuts at: 5768 6426 2400
Size: 658 5261
Fragments arranged by size:
5261 837 683 658 402 402 326 281
259 178
EcoRI G'AATT_C
Cuts at: 4282 6291 4282
Size: 2009 7278
EcoRV GAT'ATC
Cuts at: 4644 4644
Size: 9287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3066 7306 3066
Size: 4240 5047
FspI TGC'GCA
Cuts at: 7068 7068
Size: 9287
HaeII r_GCGC'y
Cuts at: 480 549 3819 6240 6721 6969 7991 8970 480
Size: 69 3270 2421 481 248 1022 979 797
Fragments arranged by size:
3270 2421 1022 979 797 481 248 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 661 693 1003 1035 1244 1276 1375
Size: 32 331 32 310 32 209 32 99
Cuts at: 1375 1407 2166 2198 2766 2798 3340 3372 4837
Size: 32 759 32 568 32 542 32 1465
Cuts at: 4837 4869 5786 5818 5861 5893 6031 6063 7129
Size: 32 917 32 43 32 138 32 1066
Cuts at: 7129 7159 7161 7191 298
Size: 30 2 30 2394
Fragments arranged by size:
2394 1465 1066 917 759 568 542 331
310 209 138 99 43 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 643 1731 5361 5708 6326 643
Size: 1088 3630 347 618 3604
Fragments arranged by size:
3630 3604 1088 618 347
HindIII A'AGCT_T
Cuts at: 722 722
Size: 9287
HpaI GTT'AAC
Cuts at: 1731 1731
Size: 9287
KasI G'GCGC_C
Cuts at: 476 6717 6965 476
Size: 6241 248 2798
Fragments arranged by size:
6241 2798 248
KpnI G_GTAC'C
Cuts at: 6307 6307
Size: 9287
MfeI C'AATT_G
Cuts at: 2536 2536
Size: 9287
MluI A'CGCG_T
Cuts at: 4034 8720 4034
Size: 4686 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2270 3461 7958 8142 2270
Size: 1191 4497 184 3415
Fragments arranged by size:
4497 3415 1191 184
MscI TGG'CCA
Cuts at: 158 1312 5308 5561 5929 6196 7048 158
Size: 1154 3996 253 368 267 852 2397
Fragments arranged by size:
3996 2397 1154 852 368 267 253
MslI CAynn'nnrTG
Cuts at: 1706 3226 3952 4267 6220 6226 7276 7403 1706
Size: 1520 726 315 1953 6 1050 127 3590
Fragments arranged by size:
3590 1953 1520 1050 726 315 127 6
MspA1I CmG'CkG
Cuts at: 363 533 786 2076 2262 3311 3473 3620 3692
Size: 170 253 1290 186 1049 162 147 72
Cuts at: 3692 4632 4991 5272 6398 6712 7072 8085 8330
Size: 940 359 281 1126 314 360 1013 245
Cuts at: 8330 363
Size: 1320
Fragments arranged by size:
1320 1290 1126 1049 1013 940 360 359
314 281 253 245 186 170 162 147
72
NaeI GCC'GGC
Cuts at: 475 7468 475
Size: 6993 2294
NarI GG'CG_CC
Cuts at: 477 6718 6966 477
Size: 6241 248 2798
Fragments arranged by size:
6241 2798 248
NcoI C'CATG_G
Cuts at: 107 330 458 1313 2345 5226 5309 5449 5556
Size: 223 128 855 1032 2881 83 140 107
Cuts at: 5556 5924 107
Size: 368 3470
Fragments arranged by size:
3470 2881 1032 855 368 223 140 128
107 83
NgoMIV G'CCGG_C
Cuts at: 473 7466 473
Size: 6993 2294
NsiI A_TGCA'T
Cuts at: 4067 4067
Size: 9287
NspI r_CATG'y
Cuts at: 1693 1879 2031 3035 3333 5009 5331 5841 6340
Size: 186 152 1004 298 1676 322 510 499
Cuts at: 6340 6840 7747 1693
Size: 500 907 3233
Fragments arranged by size:
3233 1676 1004 907 510 500 499 322
298 186 152
PciI A'CATG_T
Cuts at: 1875 5327 5837 7743 1875
Size: 3452 510 1906 3419
Fragments arranged by size:
3452 3419 1906 510
PflMI CCAn_nnn'nTGG
Cuts at: 2367 4007 4568 2367
Size: 1640 561 7086
Fragments arranged by size:
7086 1640 561
PfoI T'CCnGG_A
Cuts at: 3286 3796 4055 5088 5480 3286
Size: 510 259 1033 392 7093
Fragments arranged by size:
7093 1033 510 392 259
PmeI GTTT'AAAC
Cuts at: 6286 6286
Size: 9287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6089 6121 8426 8458 6089
Size: 32 2305 32 6918
Fragments arranged by size:
6918 2305 32 32
PpuMI rG'GwC_Cy
Cuts at: 2400 2802 3204 3485 4427 5442 5768 2400
Size: 402 402 281 942 1015 326 5919
Fragments arranged by size:
5919 1015 942 402 402 326 281
PshAI GACnn'nnGTC
Cuts at: 221 2275 4432 221
Size: 2054 2157 5076
Fragments arranged by size:
5076 2157 2054
PspOMI G'GGCC_C
Cuts at: 2357 2357
Size: 9287
PspXI vC'TCGA_Gb
Cuts at: 2932 5867 2932
Size: 2935 6352
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5041 5073 5041
Size: 32 9255
PstI C_TGCA'G
Cuts at: 2231 2810 2858 2867 4247 4838 5624 5681 6334
Size: 579 48 9 1380 591 786 57 653
Cuts at: 6334 7019 2231
Size: 685 4499
Fragments arranged by size:
4499 1380 786 685 653 591 579 57
48 9
PvuI CG_AT'CG
Cuts at: 104 4613 104
Size: 4509 4778
PvuII CAG'CTG
Cuts at: 786 2076 2262 3620 4632 4991 6712 7072 786
Size: 1290 186 1358 1012 359 1721 360 3001
Fragments arranged by size:
3001 1721 1358 1290 1012 360 359 186
RsrII CG'GwC_CG
Cuts at: 2130 7482 2130
Size: 5352 3935
SacI G_AGCT'C
Cuts at: 329 2651 2888 6301 329
Size: 2322 237 3413 3315
Fragments arranged by size:
3413 3315 2322 237
SalI G'TCGA_C
Cuts at: 5359 6324 5359
Size: 965 8322
SanDI GG'GwC_CC
Cuts at: 4427 5768 4427
Size: 1341 7946
SapI GCTCTTCn'nnn_
Cuts at: 2701 7310 7520 2701
Size: 4609 210 4468
Fragments arranged by size:
4609 4468 210
SbfI CC_TGCA'GG
Cuts at: 6334 6334
Size: 9287
ScaI AGT'ACT
Cuts at: 174 1489 8804 174
Size: 1315 7315 657
Fragments arranged by size:
7315 1315 657
SfcI C'TryA_G
Cuts at: 33 1934 2227 2806 2854 2863 4243 4834 4885
Size: 1901 293 579 48 9 1380 591 51
Cuts at: 4885 5620 5677 6240 6330 7015 8008 8199 33
Size: 735 57 563 90 685 993 191 1121
Fragments arranged by size:
1901 1380 1121 993 735 685 591 579
563 293 191 90 57 51 48 9
SfoI GGC'GCC
Cuts at: 478 6719 6967 478
Size: 6241 248 2798
Fragments arranged by size:
6241 2798 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9287
SmaI CCC'GGG
Cuts at: 873 6309 873
Size: 5436 3851
SmlI C'TyrA_G
Cuts at: 619 1646 2932 3703 3748 3877 5044 5053 5134
Size: 1027 1286 771 45 129 1167 9 81
Cuts at: 5134 5686 5867 6442 7357 7849 8111 619
Size: 552 181 575 915 492 262 1795
Fragments arranged by size:
1795 1286 1167 1027 915 771 575 552
492 262 181 129 81 45 9
SnaBI TAC'GTA
Cuts at: 6134 6134
Size: 9287
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9287
SphI G_CATG'C
Cuts at: 1693 3035 3333 6340 6840 1693
Size: 1342 298 3007 500 4140
Fragments arranged by size:
4140 3007 1342 500 298
SspI AAT'ATT
Cuts at: 810 810
Size: 9287
StuI AGG'CCT
Cuts at: 5303 5303
Size: 9287
StyI C'CwwG_G
Cuts at: 107 330 458 1313 2273 2294 2345 3929 4103
Size: 223 128 855 960 21 51 1584 174
Cuts at: 4103 4601 5226 5309 5449 5556 5924 6421 6775
Size: 498 625 83 140 107 368 497 354
Cuts at: 6775 107
Size: 2619
Fragments arranged by size:
2619 1584 960 855 625 498 497 368
354 223 174 140 128 107 83 51
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2857 4387 4639 4729 6016 6870 7003 7462 7671
Size: 1530 252 90 1287 854 133 459 209
Cuts at: 7671 2857
Size: 4473
Fragments arranged by size:
4473 1530 1287 854 459 252 209 133
90
TatI w'GTAC_w
Cuts at: 172 467 746 1487 3614 3653 3848 4365 4925
Size: 295 279 741 2127 39 195 517 560
Cuts at: 4925 4985 5933 8802 172
Size: 60 948 2869 657
Fragments arranged by size:
2869 2127 948 741 657 560 517 295
279 195 60 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2893 2925 3289 3321 8804 8836 2893
Size: 32 364 32 5483 32 3344
Fragments arranged by size:
5483 3344 364 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6043 7084 6043
Size: 1041 8246
XbaI T'CTAG_A
Cuts at: 6318 6318
Size: 9287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3166 5269 6183 6782 3166
Size: 2103 914 599 5671
Fragments arranged by size:
5671 2103 914 599
XhoI C'TCGA_G
Cuts at: 2932 5867 2932
Size: 2935 6352
XmaI C'CCGG_G
Cuts at: 871 6307 871
Size: 5436 3851
XmnI GAAnn'nnTTC
Cuts at: 4477 5084 4477
Size: 607 8680
ZraI GAC'GTC
Cuts at: 2202 2202
Size: 9287
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BmtI BsiWI BssHII BstEII
ClaI FseI FspAI NdeI NheI NotI NruI PacI
PasI PmlI PsiI SacII SexAI SfiI SgrAI SrfI
SwaI